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bull spermatozoa selected by thermotaxis exhibit high DNA integrity,specific head morphometry,and improve ICSI outcome

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Journal of Animal Science and biotechnology 2023年第3期14卷 1001-1011页

作者： sara Ruiz-Diaz Rosane Mazzarella Paula Navarrete-Lopez Raul Fernandez-Gonzalez Celia de Frutos Maria Maroto Claudia Cucala Paula beltran-brena Marta Lombo Dimitrios Rizos Alfonso Gutierrez-Adan Department of Animal Reproduction INIA-CSIC28040 MadridSpain Mistral Fertility Clinics S.L Clinica TambreCalle Tambre828002 MadridSpain

background Sperm migration by thermotaxis is a guidance mechanism that operates along the oviduct and it has proved to be a valid method for selecting spermatozoa with low DNA fragmentation(SDF)in mice,humans,and *** study aimed to analyse if bull spermatozoa could be selected by thermotaxis and to assess their quality in terms of SDF as well as determine the presence of a specific sperm subpopulation based on sperm morphometry and assess their fertilizing capacity by *** We used frozen-thawed sperm from 6 bulls and sperm selection by thermotaxis was performed with TALP medium supplemented with 25 mmol/L of HEPES and 5 mmol/L of *** these conditions,sperm selection was achieved,obtaining a net thermotaxis of 3.6%.Subsequently,we analysed the SDF of the migrated and not-migrated spermatozoa using the neutral COMET assay,and we evaluated the size of the sperm head using Hemacolor■ staining with Motic Images Plus 3 ***,migrated and not-migrated spermatozoa by thermotaxis were used to fertilize bovine in vitro matured(IVM)oocytes by ICSI,a very inefficient procedure in cattle that is only successful when the oocyte is artificially *** The results showed lower SDF(χ^(2),Pbution of sperm subpopulations structure varied between groups,highlighting cluster 2,characterized by spermatozoa with small head size,and high ellipticity and elongated heads,as the most abundant in the thermotaxis migrated *** performed ICSI(without oocyte artificial activation)with the thermotactic sperm,the blastocyst rate was 32.2%±9.3%in the group microinjected with the thermotactic spermatozoa vs.8.3%±7.8%in the group of not-migrated sperm(χ^(2),Pbull sperm selection by thermotaxis has a much higher D

关键词： ART bovine Sperm morphometry Sperm selection Thermotaxis

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CRISPR/Cas9 editing of the polygalacturonase FaPG1 gene improves strawberry fruit firmness

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Horticulture Research 2023年第3期10卷 201-210页

作者： Gloria López-Casado Cristina Sánchez-Raya Pablo D.Ric-Varas Candelas Paniagua Rosario blanco-Portales Juan Muñoz-blanco sara Pose Antonio J.Matas Jose A.Mercado Departamento de botánica y Fisiología Vegetal Instituto de Hortofruticultura Subtropical y Mediterránea‘La Mayora’(IHSM-UMA-CSIC)Universidad de Málaga29071 MálagaSpain Departamento de bioquímica y biología Molecular Universidad de Córdoba14071CórdobaSpain

Firmness is one of the most important fruit quality traits in *** postharvest shelf life of this soft fruit is highly limited by the loss of firmness,where cell wall disassembly plays an important *** studies demonstrated that the polygalacturonase FaPG1 has a key role in remodelling pectins during strawberry *** this study,FaPG1 knockout strawberry plants have been generated using the CRISPR/Cas9 system delivered via Agrobacterium *** independent lines,cv.“Chandler”,were obtained,and all of them were successfully edited as determined by PCR amplification and T7 endonuclease *** targeted mutagenesis insertion and deletion rates were analyzed using targeted deep *** percentage of edited sequences varied from 47%up to almost 100%,being higher than 95%for seven of the selected *** analyses showed that 7 out of the eight lines analyzed produced fruits significantly firmer than the control,ranging from 33 to 70%increase in *** was a positive relationship between the degree of FaPG1 editing and the rise in fruit *** changes were observed in other fruit quality traits,such as colour,soluble solids,titratable acidity or anthocyanin *** fruits showed a reduced softening rate during postharvest,displayed a reduced transpirational water loss,and were less damaged by botrytis cinerea *** analysis of four potential off-target sites revealed no mutation *** conclusion,editing the FaPG1 gene using the CRISPR/Cas9 system is an efficient method for improving strawberry fruit firmness and shelf life.

关键词： fir straw PG1

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Hemophagocytic lymphohistiocytosis caused by primary Epstein-barr virus in patient with Crohn's disease

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World Journal of Gastrointestinal Surgery 2013年第11期5卷 306-308页

作者： Francesco Virdis sara Tacci Federico Messina Massimo Varcada Department of Emergency General Surgery Royal Free HospitalLondonGreater London NW3 2QGUnited Kingdom

We present a case of a 19-year-old man with a 6-year history of Crohn's disease(CD), previously treated with 6-mercaptopurine, who was admitted to our department for Epstein-barr virus(EbV) infection and subsequently developed a hemophagocytic lymphohistiocytosis(HLH). HLH is a rare disease which causes phagocytosis of all bone marrow derived cells. It can be a primary form as a autosomic recessive disease, or a secondary form associated with a variety of infections; EbV is the most common, the one with poorer prognosis. The incidence of lymphoproliferative disorders was increased in patients with inflammatory bowel disease(IbD) treated with thiopurines. Specific EbV-related clinical and virological management should be considered when treating a patient with IbD with immunosuppressive therapy. Moreover EbV infection in immunosuppressed patient can occur with more aggressive forms such as encephalitis and diffuse large b cell lymphoma. Our case confirms what is described in the literature; patients with IbD, particularly patients with CD receiving thiopurine therapy, who present 5 d of fever and cervical lymphadenopathy or previous evidence of lymphopenia should be screened for HLH.

关键词： Crohn’s disease Hemophagocytic lymphohistiocytosis Epstein-barr virus infection Immunosupressive therapy Thiopurines

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Effect of Different Calcination Process and Gd<sub>2</sub>O<sub>3</sub>as Impurities on the Different Phases of bi-based Superconductor

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World Journal of Condensed Matter Physics 2012年第3期2卷 148-152页

作者： Ahmad Amirabadizadeh sara Memarzadeh Naser Tajabor Hadi Arabi Department of Physics Faculty of Science Fer-dowsi University of Mashhad Mashhad Iran Department of Physics Faculty of Science University of Birjand Birjand Iran

In this research two samples of a nominal composition bi1.6Pb0.4Sr2Ca2Cu3Ox were prepared by the solid state reaction method, using two different routes. Codes A and b are used to refer to the two samples. In preparing sample A, the standard method for calcination was used, while for sample b, the SrCO3 and CaCO3 were calcinated at 1100℃ for 3 h. Calcination was done separately on SrCO3 and CaCO3 in order to eliminate CO2. Then after mixing and grinding, the powder mixtures of bi2O3, CuO, PbO, SrO and CaO were calcinated at 840℃ for 70 h. Also, the samples (samples C) of nominal compositions bi1.6Pb0.4Sr2Ca2Cu3Ox+ % x Gd2O3 (% x = 0, 3, 5, 7 and 9) have been synthesized by this method. The XRD and SEM results show that addition of Gd2O3 helps to increase the amount of bi-2212 phase, while alternative calcination process improves the formation of the bi-2223 phase. In addition, the AC susceptibility measurements confirm the XRD and SEM results.

关键词： bi-Pb-Sr-Ca-Cu-O System X-Ray Diffraction Scanning Electron Microscopy AC Susceptibility

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Retrospective Cohort Study on Acute Care in Obstetrics and Gynecology: Analogies and Differences When Compared to Emergency Medicine

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Open Journal of Obstetrics and Gynecology 2017年第4期7卷 473-485页

作者： Giuseppe Chiossi Stefano Palomba sara b.lduzzi Maged M. Costantine Angela I. Falbo Anna M. Ferrari Giovanni b. La Sala Department of Obstetrics and Gynecology University of Texas Medical Branch Galveston TX USA Department of Obstetrics and Gynecology Arcispedale Santa Maria Nuova IRCCS Reggio Emilia Italy Statistics Unit Department of Oncology Hematology and Respiratory Diseases University of Modena and Reggio Emilia Modena Italy Department of Emergency Medicine Arcispedale Santa Maria Nuova IRCCS Reggio Emilia Italy University of Modena and Reggio Emilia via Università 4 Modena Italy

Introduction: the demand for urgent obstetric and gynecology care has progressively increased: in the United States approximately 1.4 million gynecologic visits are made to the emergency department (ED) annually, while almost 75% of women make at least 1 unscheduled visit during pregnancy. Moreover, research has recently focused on setting standards in unscheduled care, and developing quality indicators to improve patients’ health. Therefore, we investigated the characteristics of women with acute gynecological or pregnancy complaints using quality indicators developed for emergency medicine, to better define the needs of this population and improve care. Methods: Retrospective cohort study on ED, and Obstetrics and Gynecology (ObGyn) triage visits, at a tertiary care hospital in Italy, during 2012. Data were analyzed with population-averaged logistic regression and Poisson regression. Results: When compared to the 33,557 ED visits, the 9245 ObGyntriage referrals were more frequently associated with pregnancy (≤12 weeks’ gestation, OR: 30.7, 95%CI;24.5 - 38.4;>12 weeks’ gestation, OR 81.2, 95%CI;64.8 - 101.4), vaginal bleeding (OR 156.6, 95%CI;82.7 - 294.4), diurnal (night access OR 0.87, 95% CI;0.78 - 0.96) and weekday access (holiday access OR 0.87, 95%CI;0.78 - 0.95), frequent users (recurrent ED visits IRR 0.87, 95%CI;0.83 - 0.9) and lower hospital admissions (ED admission OR 1.6, 95%CI;1.4 - 1.8). Conclusion: ObGyn triage patients differed from ED users, and were at higher risk of “crowding”. Such diversities should be considered to improve female healthcare services and allocate resources more efficiently.

关键词： Acute Care Obstetric Urgencies Gynecologic Urgencies Obstetrics and Gynecology Triage Emergency Department

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Evaluating indicators of human well-being for ecosystem-based management

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Ecosystem Health and Sustainability 2017年第12期3卷 2-19页

作者： sara Jo breslow Margaret Allen Danielle Holstein brit Sojka Raz barnea Xavier basurto Courtney Carothers Susan Charnley arah Coulthard Nives Dolsak Jamie Donatuto Carlos Garcia-Quijano Christina C.Hicks Arielle Levine Michael b.Masci Karma Norman Melissa Poe Terre Satterfield Kevin St.Martin Phillip S.Levin Center for Creative Conservation University of WashingtonSeattleWAUSA School of Marine and Environmental Affairs University of WashingtonSeattleWAUSA Nicholas School of the Environment Duke UniversityBeaufortNCUSA College of Fisheries and Ocean Sciences University of Alaska FairbanksAnchorageAKUSA Pacific Northwest Research Station USDA Forest ServicePortlandORUSA Department of Social Sciences and Languages Northumbria UniversityNewcastle upon TyneUK Community Environmental Health Program Swinomish Indian Tribal CommunityLa ConnerWAUSA Department of Sociology and Anthropology The University of Rhode IslandKingstonRI USA Center for Ocean Solutions Stanford UniversityMontereyCAUSA Lancaster Environment Center Lancaster UniversityLancasterUK Department of Geography San Diego State UniversitySan DiegoCAUSA Moore Center for Science Conservation InternationalArlingtonVAUS Northwest Fisheries Science Center National Oceanic and Atmospheric AdministrationSeattleWAUSA Washington Sea Grant University of WashingtonSeattleWAUSA Institute for Resources Environment and SustainabilityUniversity of British ColumbiaVancouverBCCanada Department of Geography RutgersThe State University of New JerseyPiscatawayNJUSA School of Environmental and Forest Sciences University of WashingtonSeattleWAUSA

Introduction:Interrelated social and ecological challenges demand an understanding of how environmental change and management decisions affect human *** paper out-lines a framework for measuring human well-being for ecosystem-based management(EbM).We present a prototype that can be adapted and developed for various scales and *** and managers use indicators to assess status and trends in integrated ecosystem assessments(IEAs).To improve the social science rigor and success of EbM,we developed a systematic and transparent approach for evaluating indicators of human well-being for an ***:Our process is based on a comprehensive conceptualization of human well-being,a scalable analysis of management priorities,and a set of indicator screening criteria tailored to the needs of *** tested our approach by evaluating more than 2000 existing social indicators related to ocean and coastal management of the US West *** focused on two foundational attributes of human well-being:resource access and *** and Discussion:Our results suggest that existing indicators and data are limited in their ability to reflect linkages between environmental change and human well-being,and extremely limited in their ability to assess social equity and *** reveal a critical need for new social indicators tailored to answer environmental questions and new data that are disaggregated by social variables to measure *** both,we stress the importance of collaborating with the people whose well-being is to be ***:Our framework is designed to encourage governments and communities to carefully assess the complex tradeoffs inherent in environmental decision-making.

关键词： Human well-being indicators ecosystem-based management integrated ecosystem assessment resource access self-determination

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High resolution colonoscopy in a bowel cancer screening program improves polyp detection

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World Journal of Gastroenterology 2011年第38期17卷 4308-4313页

作者： Matthew R banks Rehan Haidry M Adil butt Lisa Whitley Judith Stein Louise Langmead Stuart L bloom Austin O' bichere sara McCartney Kalpesh basherdas Manuel Rodriguez-Justo Laurence b Lovat Department of Gastrointestinal Services University College London Hospitals NHS Foundation Trust Department of Histopathology University College London Hospitals NHS Foundation Trust

AIM： To compare high resolution colonoscopy （Olympus Lucera） with a megapixel high resolution system （Pentax HiLine） as an in-service evaluation. METHODS： Polyp detection rates and measures of performance were collected for 269 colonoscopy procedures. Five colonoscopists conducted the study over a three month period, as part of the United Kingdom bowel cancer screening program. RESULTS：There were no differences in procedure duration （x^2 p = 0.98）, caecal intubation rates （x^2 P = 0.67）, or depth of sedation （x^2 P = 0.64）. Mild discomfort was more common in the Pentax group （x^2 p = 0.036）. Adenoma detection rate was significantly higher in the Pentax group （x^2 test for trend P = 0.01）. Most of the extra polyps detected were flat or sessile adenomas. CONCLUSION： Megapixel definition colonoscopes improve adenoma detection without compromising other measures of endoscope performance. Increased polyp detection rates may improve future outcomes in bowel cancer screening programs.

关键词： High resolution colonoscopy bowel cancerscreening Polyp detection

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Electron Micrographic Representations of Mechanisms of Action of Murine Norovirus on ATCC TIb-71 Cells and Level of Gene Expression

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Advances in Microbiology 2023年第1期13卷 32-47页

作者： Uchenna b. Iloghalu sara E. Miller Akamu J. Ewunkem Janak R. Khatiwada Shurrita S. Davis Leonard L. Williams Department of biology North Carolina A&T State University Greensboro USA Department of biology Guilford College Greensboro USA Department of Pathology Duke University Durham USA Department of biological Sciences Winston-Salem State University Winston-Salem USA Center for Excellence in Post-Harvest Technologies Kannapolis North Carolina A&T State University Greensboro USA

Human noroviruses (HuNoV) are the number one cause of viral gastroenteritis worldwide resulting in a significant cause of morbidity and mortality in all age groups. However, despite the medical relevance of HuNoV, effective treatment against norovirus infection is yet to be developed. In this study, we investigated the anti-Noroviral activity of Hibiscus sabdariffa (HS) calyces and Zanthoxylum armatum (ZA) seeds using murine norovirus, a surrogate of human norovirus. The antiviral mechanisms of action were also examined using a gene expression studies and transmission electron microscopy. Our results showed that virus-infected cells were left potentially void of all the cell machineries whereas uninfected cells represent healthy normal and dividing cells. The infected treated cells with extracts showed restoration of the dense cytoplasm, cytoplasmic membrane, and the nucleus. These cells were also associated with the expression of ORF genes. This study demonstrates the antiviral properties of Hibiscus sabdariffa (HS) calyces and Zanthoxylum armatum (ZA) and thus indicates their potential as natural remedies to treat noroviruses.

关键词： Norovirus Plant Extracts Transmission Electron Microscope Prevention/Control

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MicroRNAs as a potential prognostic factor in gastric cancer

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World Journal of Gastroenterology 2011年第35期17卷 3976-3985页

作者： baruch brenner Moshe b Hoshen Ofer Purim Miriam ben David Karin Ashkenazi Gideon Marshak Yulia Kundel Ronen brenner sara Morgenstern Marisa Halpern Nitzan Rosenfeld Ayelet Chajut Yaron Niv Michal Kushnir Institute of Oncology Davidoff CenterRabin Medical CenterBeilinson HospitalPetach Tikva 49100Israel Sackler Faculty of Medicine Tel Aviv UniversityTel Aviv 69978Israel Rosetta Genomics Ltd. Rehovot 76706Israel Clalit Research Institute Clalit Medical ServicesTel Aviv 69978Israel Institute of Oncology Rabin Medical CenterGolda-Hasharon HospitalPetach Tikva 49100Israel Institute of Oncology Rabin Medical CenterBeilinson HospitalPetach Tikva 49100Israel Institute of Pathology Rabin Medical CenterBeilinson HospitalPetach Tikva 49100Israel Institute of Pathology Rabin Medical CenterGolda-Hasharon HospitalPetach Tikva 49100Israel Cancer Research UK Cambridge Research InstituteCambridge CB2 OREUnited Kingdom Department of Gastroenterology Rabin Medical CenterBeilinson HospitalPetach Tikva 49100Israel Sackler Faculty of Medicine Tel Aviv UniversityTel Aviv 69978 Israel

AIM:To compare the microRNA (miR) profiles in the primary tumor of patients with recurrent and non-recurrent gastric ***:The study group included 45 patients who underwent curative gastrectomies from 1995 to 2005 without adjuvant or neoadjuvant therapy and for whom adequate tumor content was *** RNA was extracted from formalin-fixed paraffin-embedded tumor samples,preserving the small RNA *** profiling using miR microarrays was performed to identify potential biomarkers of recurrence after *** expression of the differential miRs was later verified by quantitative real-time polymerase chain reaction (qRT-PCR).Findings were compared between patients who had a recurrence within 36 mo of surgery (bad-prognosis group,n=14,31%) and those who did not (good-prognosis group,n=31,69%).RESULTS:Three miRs,miR-451,miR-199a-3p and miR-195 were found to be differentially expressed in tumors from patients with good prognosis vs patients with bad prognosis (Pboth recurrence and *** miR-451,the positive predictive value for non-recurrence was 100% (13/13).The expression of the differential miRs was verified by qRT-PCR,showing high correlation to the microarray data and similar separation into prognosis ***:This study identified three miRs,miR-451,miR-199a-3p and miR-195 to be predictive of recurrence of gastric *** these,miR-451 had the strongest prognostic impact.

关键词： MicroRNA Prognosis Recurrence Gastric cancer

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基于模糊逻辑的智能驾驶关键技术研究

基于模糊逻辑的智能驾驶关键技术研究

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作者： sara Saved M.Abdelkader 湖南大学

学位级别：硕士

在过去的30年,用于城市地面交通工具中自动驾驶技术的开发已经得到了飞速的发展。目前,现代自主驾驶车辆已具备一定感知车辆周围环境的能力,比如根椐分类所分析对象的类型并进行检测;观测周身环境的变化并评估对象的移动可能性;在遵循... 详细信息

在过去的30年,用于城市地面交通工具中自动驾驶技术的开发已经得到了飞速的发展。目前,现代自主驾驶车辆已具备一定感知车辆周围环境的能力,比如根椐分类所分析对象的类型并进行检测;观测周身环境的变化并评估对象的移动可能性;在遵循交通法规的基础之上对复杂的交通现状进行车辆路径规划并分析障碍物的移动方向等。在这些复杂的情况下,这种自主导航能力是建立在很多学科（例如：计算机学、电子工程学、机器人技术和控制学等）的基础之上跨越并结合之后研发的。本学位论文对自主车辆系统所包含很多重叠和整合系统及技术知识进行了探讨,并对从摄像机镜头捕捉到的图像以及对于车辆位置、车辆性质种类、不同物体的速度及来自从各种传感器如GPS、雷达、相机、和其他人对车辆周围的危险指数分析并对相关数据资料加以分析和总结。值得说明的是对所研究对象来判断并得出详细描述他们的内容研究（比如根椐他们的外部形象特征来将其归类）不在本论文的范围内。首先我们设计或规划得出描述每个对象的N个特征向量等数据,每个特征向量包含的数据描述如下：（1）对象属性（比如汽车、人群、树木、障碍物等等）（2）测试车辆和物体之间的距离以[以（x,y）坐标轴的方式描述与计算]。（3）运动客体的相对移动方向[以（x,y）坐标轴的方式描述与计算]。（4）行驶车辆的当前速度。行驶环境范围内,车辆与每个物体之间计算得出的时间值可能会有冲突,这是证明风险的关键点。基于可能存在的碰撞时间标准,每个对象都是通过使用不同的分类技术风险和危险水平来排序。目前这些技术具体包含：K最近邻群集算法（KNN）、神经网络（NN）、支持向量机（SVM）等。相比较之下,K-means算法经研究被验证为最适用于本文所描述数据集的最好方法。然后,我们研究如何处理那些仅使用单项输入模式可能无法实现的、并从多个特征向量中为了提高准确性和更具体推论规则和推理的数据融合系统相结合的数据,以及为了给予一个正确精准的决策而需要对n向量操控和整合等方面的问题。为了计算给出一个正确的决策信息,我们针对一个由F个自定义的特征组成向量,并结合N个采集对象特征向量,然后融合集成所有必需的数据集。在此研究背景的框架下,我们提出了两种不同的融合算法： A)基于车辆相对位置的决策级数据融合[最高风险从（左,右和前面,后面）]。 b)基于对象特征属性的数据融合[风险最高的(汽车、人力、交通信号灯、和障碍]。并且,在此基础上我们详细比较了这两个方法。同时在决策阶段时依据输入的特性选用其中之一方法,以便得到较好的决策结果。最后,我们介绍了提出的模糊逻辑控制系统,用于解决不确定性的考虑多个选择的问题,一种合适的模糊逻辑决策算法。该算法的思想是依据不同行为特征选择导致的有关车辆位置等不同距离特征的影响,并以计算得出的汽车碰撞时间进行测试。不同距离特征会影响所需计算的汽车碰撞时间,这个运算法则始于那些行为被选择,而后执行“并”的法则完成和遵循。系统描述了汽车自主行驶决策的各种工况,包括每个“仅在前”,“仅在后”,“仅在左”,“仅在右”等方向的工况下车辆对象可能产生事故的原因。由于本文方法具备了提示正确的方向转向和适当的速度来避免碰撞的能力,这项研究结果将进一步提高影响车辆驾驶人和自主驾驶代理未来的自主驾驶决策和辨识能力。

关键词：自主驾驶技术模糊逻辑数据融 K最近邻算法神经元网络支持向量机

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