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Countrywide Survey for MERS-Coronavirus Antibodies in Dromedaries and Humans in Pakistan

Virologica Sinica 2018年第5期33卷 410-417页

作者： Ali Zohaib Muhammad saqib Muhammad Ammar Athar Jing Chen Awais-ur-Rahman Sial Saeed Khan Zeeshan Taj Halima Sadia Usman Tahir Muhammad Haleem Tayyab Muhammad Asif Qureshi Muhammad Khalid Mansoor Muhammad Ahsan Naeem Bing-Jie Hu Bilal Ahmed Khan Ikram Din Ujjan Bei Li Wei Zhang Yun Luo Yan Zhu Cecilia Waruhiu Iahtasham Khan Xing-Lou Yang Muhammad Sohail Sajid Victor Max Corman Bing Yan Zheng-Li Shi CAS Key Laboratory of Special Pathogens and Biosafety Wuhan Institute of VirologyChinese Academy of SciencesWuhan 430071China University of Ctunese Academy of Sciences Beijing 100049China Faculty of Veterinary Medicine University of AgricultureFaisalabad 38040Pakistan Laboratory of Cancer Biomarkers and Liquid Biopsy College of PharmacyHenan UniversityHenan 475004China Department of Clinical Studies Faculty of Veterinary and Animal SciencesPMAS-Arid Agriculture UniversityRawalpindi 46000Pakistan Dow University of Health Science Pakistan Karachi 74200 Department of Microbiology Government College University FaisalabadFaisalabad 38000Pakistan Livestock and Dairy Development Department(L&DD) Lahore 54000Pakistan College of Veterinary and Animal Sciences The Islamia University of BahawalpurBahawalpur 63100Pakistan State Key Laboratory of Agricultural Mlcroblology Huazhong Agricultural UniversityWuhan 430070China Liaqat University of Medical and Health Science Pakistan Jamshoro 76090 Section of Epidemiology and Public Health Department of Chnical SciencesUniversity of Veterinary and Animal SciencesLahore subcampusJhang 35200Pakistan Institute of Virology Chadte-Universitatsmedizin BerlinCorporate member of Free UniversityHumboldt-University and Berlin Institute of HealthBerlin 10117Germany German Center for Infection Research(DZIF) Berlin 10117Germany

Middle East Respiratory Syndrome Coronavirus (MERS-CoV) is a zoonotic pathogen capable of causing severe respiratory disease in humans. Although dromedary camels are considered as a major reservoir host, the MERS-CoV infection dynamics in camels are not fully understood. Through surveillance in Pakistan, nasal (n = 776) and serum (n = 1050)samples were collected from camels between November 2015 and February 2018. Samples were collected from animal markets, free-roaming herds and abattoirs. An in-house ELISA was developed to detect IgG against MERS-CoV. A total of 794 camels were found seropositive for MERS-CoV. Prevalence increased with the age and the highest seroprevalence was recorded in camels aged [ 10 years (81.37%) followed by those aged 3.1–10 years (78.65%) and B 3 years (58.19%).Higher prevalence was observed in female (78.13%) as compared to male (70.70%). Of the camel nasal swabs, 22 were found to be positive by RT-qPCR though with high Ct values. Moreover, 2,409 human serum samples were also collected from four provinces of Pakistan during 2016–2017. Among the sampled population, 840 humans were camel *** we found a high rate of MERS-CoV antibody positive dromedaries (75.62%) in Pakistan, no neutralizing antibodies were detected in humans with and without contact to camels.

关键词： Middle East Respiratory Syndrome Coronavirus (MERS-CoV) Camel -Human Pakistan

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Geochemistry and Tectonic Settings of Felsic Dykes in the Neoproterozoic Nagar Parkar Igneous Complex, SE Sindh, Pakistan

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Acta Geologica Sinica(English Edition) 2016年第S1期90卷 124-页

作者： Tahseenullah Khan Mamoru Murata M.Qasim Jan Hafiz Ur Rehman Muhammad Zafar Hiroaki Ozawa Anwar Qadir saqib Mehmood Department of Earth and Environmental Sciences Bahria University Department of Geosciences Faculty of Science Naruto University of Education National University Corporation National Centre of Excellence in Geology University of Peshawar and COMSTECH Islamabad Department of Earth and Environmental Science Faculty of Science Kagoshima University International Cooperation Center for the Teacher Education and Training Naruto University of Education Department of Geology Haripur University

The Nagar Parkar complex consists of Neoproterozoic igneous and metamorphic rocks dissected by episodic mafic and felsic *** latter can be classified broadly into porphyritic felsic dykes and aplitic felsic dykes(minor)

关键词： SE Sindh Pakistan Geochemistry and Tectonic Settings of Felsic Dykes in the Neoproterozoic Nagar Parkar Igneous Complex

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Development of Gene Expression and Gene Silencing Vectors Based onCotton Leaf Curl Virus for Functional Genomics in Cotton

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棉花学报 2002年第S1期14卷 45-45页

作者： Shahid MANSOOR Muhammad saqib Mazhar HUSSAIN Rob BRIDDON Kauser A.MALIK Yusuf ZAFAR National Institule for Biotechnology and Genetic Engineering(NIBGE) P O B 577FaisalabadPakistan Department of Disease and Stress Biology John Innes CentreNorweichNR47UHUnited Kingdom

Cotton leaf curl virus is the first example of acotton-infecting virus where infectious clones *** viruses are valuable tools inunderstanding plant biology as they can beengineered for expression of foreign genes orsi... 详细信息

关键词：抗卷叶病毒棉花基因组研究基因表达基因沉默载体构建 Functional Genomics Gene Silencing

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CZTSSe薄膜太阳能电池:Zn/Sn比例的优化

CZTSSe薄膜太阳能电池:Zn/Sn比例的优化

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第七届新型太阳能电池材料科学与技术学术研讨会

作者：葛思洁徐菡杨文韬黄宇翔 saqib Nawaz Khan 林显忠中山大学聚合物复合材料及功能材料教育部重点实验室材料科学与工程学院

理论计算表明CZTSSe薄膜中与Sn相关的缺陷多为深能级缺陷,不利于载流子的传输,同时该类缺陷的形成能可通过薄膜成分进行调控。在本文中,我们通过调控基于DMF和DMSO混合溶剂的前驱体溶液中Zn/Sn比例(1.0到1.9),研究Zn/Sn比对所得CZTSSe... 详细信息

理论计算表明CZTSSe薄膜中与Sn相关的缺陷多为深能级缺陷,不利于载流子的传输,同时该类缺陷的形成能可通过薄膜成分进行调控。在本文中,我们通过调控基于DMF和DMSO混合溶剂的前驱体溶液中Zn/Sn比例(1.0到1.9),研究Zn/Sn比对所得CZTSSe薄膜的结晶性、电流传输及电池性能的影响。通过SEM观察得到,薄膜结晶性在一定Zn/Sn比例范围内随之增加而提高,晶粒尺寸随Zn/Sn比例的增加而增大,但与此同时薄膜的表面粗糙度也逐渐增大,Zn/Sn比例为1.7时,薄膜表面晶粒开始出现不连续状态;ICP和XPS测试结果表明,即使前驱体薄膜的Zn/Sn高达1.9,退火后薄膜表面Zn/Sn比可自调节到1.05至1.27的范围内;随着Zn/Sn增加,薄膜中会形成ZnSe二次相,存在于薄膜的不同的位置,当其主要覆于薄膜内部晶界时,可促进载流子传输,但当其过多堆积于薄膜表面或底部时,将提高电池串阻,恶化界面接触,阻碍载流子传输。此外,Zn/Sn增加可有效抑制MoSe2层的厚度,进一步优化了背界面接触。更重要的是,当Zn/Sn比例从1.0提高至1.5时,开路电压损失由730mV显著地降至580m,电池的效率也从4.5%提高到了10.0%,并且在较大的Zn/Sn比例范围内仍能保持9%左右的效率,然而Zn/Sn比例增至1.9时,效率下降到4.3%。该工作对今后进一步提高电池性能具有重要的参考意义。

关键词： Zn/Sn CZTSSe薄膜

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