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基于辣椒基因组测序探究辣味在种群中的进化

辣椒杂志 2014年第2期12卷 40-50页

作者： Seungill Kim Minkyu park Seon-In Yeom Yong-Min Kim Je Min Lee Hyun-Ah Lee Eunyoung Seo Jaeyoung Choi Kyeongchae Cheong Ki-Tae Kim Kyongyong Jung Gir-Won Lee sang-Keun Oh Chungyun Bae Saet-Byul Kim Hye-Young Lee Shin-Young Kim Myung-Shin Kim Byoung-Cheorl Kang Yeong Deuk Jo Hee-Bum Yang Hee-Jin Jeong Won-Hee Kang Jin-Kyung Kwon Chanseok Shin Jae Yun Lim June Hyun park Jin Hoe Huh June-Sik Kim Byung-Dong Kim Oded Cohen Ilan Paran Mi Chung Suh Saet Buyl Lee Yeon-Ki Kim Younhee Shin Seung-Jae Noh Junhyung park Young Sam Seo Suk-Yoon Kwon Hyun A Kim Jeong Mee park Hyun-Jin Kim sang-Bong Choi Paul W Bosland Gregory Reeves Sung-Hwan Jo Bong-Woo Lee Hyung-Taeg Cho Hee-Seung Choi Min-Soo Lee Yeisoo Yu Yang Do Choi Beom-Seok park Allen van Deynze Hamid Ashrafi Theresa Hill Woo Taek Kim Hyun-Sook Pai Hee Kyung Ahn Inhwa Yeam James J Giovannoni Jocelyn K C Rose Iben Sφrensen sang-Jik Lee Ryan W Kim Ik-Young Choi Beom-soon Choi Jong-Sung Lim Yong-Hwan Lee Doil Choi 刘峰张西露 Department of Plant Science Seoul National University Plant Genomics and Breeding Institute Seoul National University Department of Agricultural Biotechnology Seoul National University Department of Bioinformatics and Life Science Soongsil University Vegetable Breeding Research Center Seoul National University Agricultural Research Organization Institute of Plant Science Volcani Center Bet DaganIsrael. Agricultural Research Organization Institute of Plant Science Volcani Center Bet DaganIsrael Department of Bioenergy Science and Technology Chonnam National University Genomics Genetics Institute GreenGene BioTech Inc. Codes Division Insilicogen Inc. Ginseng Resources Research Laboratory Korea Ginseng Corporation Korea Research Institute of Bioscience and Biotechnology Division of Bioscience and Bioinformatics Myongji University Department of Plant and Environmental Sciences New Mexico State University Chile Pepper Institute New Mexico State University Seeders Inc. Department of Biological Sciences Seoul National University Arizona Genomics Institute University of Arizona Agricultural Genome Center National Academy of Agricultural Science Rural Development Administration Seed Biotechnology Center University of California Davis Davis California USA Department of Systems Biology Yonsei University Department of Horticulture and Breeding Andong National University US Department of Agriculture-Agricultural Research Service Robert W. Holley Center Ithaca New York USA Boyce Thompson Institute for Plant Research Cornell University Ithaca New York USA Department of Plant Biology Cornell University Ithaca New York USA Biotechnology Institute Nongwoo Bio Yeoju Korea Genome Center University of California Davis Davis California USA National Instrumentation Center for Environmental Management Seoul National University Seoul Korea 湖南省蔬菜研究所

辣椒(Capsicum annuum)是美洲最古老的驯化作物之一,在全世界被广泛种植。本研究对CM334(墨西哥地方品种)进行了全基因组测序和组装(覆盖度为186.6×),同时还对另外两个栽培种和一个野生种分别进行了重测序和从头(de novo)测序。结果表... 详细信息

辣椒(Capsicum annuum)是美洲最古老的驯化作物之一,在全世界被广泛种植。本研究对CM334(墨西哥地方品种)进行了全基因组测序和组装(覆盖度为186.6×),同时还对另外两个栽培种和一个野生种分别进行了重测序和从头(de novo)测序。结果表明辣椒基因组大小约为番茄的4倍,辣椒基因组上存在大量gypsy转座子和花椰菜病毒家族元件的积累。结合转录组综合分析认为,辣椒素合成酶基因表达模式的改变及新功能化是导致辣椒素生物合成的原因,研究还发现辣椒与番茄在乙烯合成及果实成熟调控方面存在明显不同的分子模式。辣椒参考基因组的发布为辣椒营养品质和药用价值的改善提供了重要平台。

关键词：基因组测序辣椒素参考基因 Capsicum 转座子辣椒果实分子模式生物合成基因表达模式基因组大小

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大米饮料制备工艺研究

食品与机械 2005年第6期21卷 68-70,78页

作者：金海珠叶向库付学军杨建荣赵玉平郑舜泽朴亮均烟台大学食品科学与工程研究所山东烟台264005 木浦大学校食品产业技术研究中心国家重点实验室

利用糙米和精米经烘炒、生物酶解、过滤、罐装、包装等工序制造色泽清亮、香气宜人、保留糙米和精米原有成分的甘甜可口的大米饮料。对烘烤时间及水解条件等关键工序作了较为系统的研究,大米最佳烘烤条件:白米190℃、20min效果较好,糙米... 详细信息

利用糙米和精米经烘炒、生物酶解、过滤、罐装、包装等工序制造色泽清亮、香气宜人、保留糙米和精米原有成分的甘甜可口的大米饮料。对烘烤时间及水解条件等关键工序作了较为系统的研究,大米最佳烘烤条件:白米190℃、20min效果较好,糙米180℃、20min。最佳水解条件为蛋白水解酶2%,葡萄糖化酶125U/g,温度50℃,时间7h。

关键词：精米糙米饮料 α-淀粉酶葡萄糖化酶蛋白酶

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韩国茶叶生产与消费(英文)

茶叶 2000年第Z1期26卷 287-298页

作者：朴龙求 Lab. of Forest Genetics Kyungpook National University Taegu 702-701 Republic of Korea

Mr. Taeryum, an envoy of Shilla Dynasty brought tea seeds from China during Tang Dynastyand the seeds were planted at Jiri Mt. by the order of King Heungduk at AD 828. During Koryo Dynasty (918-1392), Buddhism spread rapidly all over the country and the tea culture reached its highest stage of prosperity. At the Chosun Dynasty. However, the ceremonial drinking of tea vanished almost completely due to the flourishing Confucian tradition, a kind of substitution of Buddhism. But a few people have supported the traditional tea culture by themselves. Since the independence of Korea soon after the World War II(1945), the cultivation area of tea plants has been increased and the cultural tradition of tea drinking has become popular *** present, the cultivation area has been continued to increase and tea production has also been lifted up year byyear. In spite of continuous increased tea cultivation area, there still is a severe problem of shortage of tea in thecountry. Tea farmers and the Korean government should take several actions to solve to remedy the *** this paper the prospect for shortage of tea demand is discussed after 2003 when the high special customs du-ties for tea products is lowered from 536% to 30 or 40% in Korea and the trade situation of tea products oncountries around Korea are also discussed.

关键词： tea shortage tea culture tea production tea consumption

RNA干扰技术和家蚕杆状病毒表达系统在纤维素酶研究中的应用前景

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蚕桑通报 2009年第2期40卷 5-10页

作者：李兴华杨华军 B.Roy E.Y.park 江丽军王丹缪云根浙江大学动物科学学院浙江杭州310029 日本静冈大学农学部应用生化系

植物通过光合作用产生的纤维素是地球上最丰富、最廉价的可再生资源。纤维素酶是糖苷水解酶的一种,能有效地将农作物秸杆等富含纤维素的物质水解为葡萄糖,进而发酵产生生物乙醇,从而解决农业、再生能源以及环境污染等问题。随着生物化... 详细信息

植物通过光合作用产生的纤维素是地球上最丰富、最廉价的可再生资源。纤维素酶是糖苷水解酶的一种,能有效地将农作物秸杆等富含纤维素的物质水解为葡萄糖,进而发酵产生生物乙醇,从而解决农业、再生能源以及环境污染等问题。随着生物化学、分子生物学以及基因工程等多种交叉学科的快速发展,获得适合工业化的高活力的纤维素酶指日可待。RNA干扰是利用双链RNA特异性地降解相应序列的mRNA,从而特异性地阻断相应基因的表达,是后基因组时代的一种强有力的调控目的基因表达的实验工具。家蚕杆状病毒表达系统是一种快速、高效表达外源基因的技术手段,目前该系统的发展和应用已经非常成熟。本文综述了纤维素酶的特性以及近年来国内外对纤维素酶、RNA干扰和家蚕杆状病毒表达系统的研究进展,并对该两种实验技术手段在今后纤维素酶研究中的应用前景作了预测和展望。

关键词：纤维素酶 RNA干扰家蚕杆状病毒表达系统

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新式乳头切开刀下的乳头括约肌切开术

世界核心医学期刊文摘（胃肠病学分册） 2006年第2期2卷 43-43页

作者： park S.-H. Kim H.-J. Do H.P. 徐瑞 Division of Gastroenterology Clinical Research Institute Soon Chun Hyang University Cheonan Hospital 23- 20 Bongmyung- Dong Cheonan Choongnam 330- 721 South Korea Dr.

Background: A new papillotome has been developed, an isolated-tip needle-knife papillotome (Iso-Tome) that has a semi-oval-shaped tip of epoxide adhesive to prevent electric leakage from the tip of the incising needle. The coated tip aids in keeping the papillotome tightly in the orifice of the ampulla of Vater and is believed to prevent unintentional deep cuts or perforations. This study was done to evaluate the clinical usefulness of the new papillotome for pre-cut papillotomy. Methods: From June 2003 to November 2003,115 patients underwent attempted ERCP. If biliary cannulation failed by the conventional method, pre-cut papillotomy was performed by using the Iso-Tome in the direction of the bile duct. After successful bile-duct cannulation, papillotomy was extended for therapeutic procedures, such as stone removal or stent insertion, in the majority of the cases. Post-ERCP complications were classified according to consensus guidelines. Observations: Pre-cut papillotomy with the Iso-Tome was done in 25 patients (21.7% of cases). Protective pancreatic stents were not used. Of these patients, 11 had common bile duct stones, 7 had pancreatitis, 5 had malignancies, one had sphincter of Oddi dysfunction, and one had bile-duct leak. After pre-cut papillotomy with the Iso-Tome, bile-duct cannulation was successfully achieved in 23 of 25 (92% ) patients. Complications occurred in 7 of 25 patients (28% ) and consisted of mild pancreatitis in 5 (20% ), moderate bleeding in one (4% ), and biliary pain in one (4% ). All 7 patients with complications were managed medically, and there was no death. Conclusions: In this pilot study, the isolated-tip needle knife was found to be a clinically useful papillotome for pre-cut papillotomy. Further large comparative studies (with and without pancreatic protective stents) are needed to determine improved efficacy and safety compared with standard techniques.

关键词：乳头切开刀括约肌功能内镜逆行总胆管结石胆石壶腹部电极头插管方法临床有效性中度出血