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Evaluation of parasitemia by qPCR in patients with chronic Chagas disease treated with benznidazole

Asian Pacific Journal of Tropical Medicine 2023年第12期16卷 565-572页

作者： Tycha Bianca Sabaini Pavan Eros Antônio de Almeida Rodrigo Gonçalves de Lima Jamiro da Silva Wanderley Luiz Cláudio Martins sandra cecília botelho costa Gláucia Elisete Barbosa Marcon Departamento de Clínica Médica Faculdade de Ciências MédicasUniversidade Estadual de Campinas(Unicamp)CampinasSão PauloBrasil Fundação Oswaldo Cruz Fiocruz Mato Grosso do SulCampo GrandeMSBrasil

Objective:To evaluate parasitemia by qPCR in patients undergoing etiological treatment and followed in a Brazilian reference ***:Parasite load was quantified by qPCR in 32 participants with chronic Chagas disease who were treated with *** analyses were performed before and after the treatment and parasite loads were compared prior and 12/18 months post the ***:Thirty-two participants were recruited and treated with benznidazole,and 20 were *** events(AE)were observed in 22 out of 29 participants that had safety data(76%),and dermatological alterations were the most frequently observed *** the 20 participants analyzed,13 and 7 completed 12 and 18 months follow-up after the treatment,respectively.12 Months after the final treatment,Trypanosoma cruzi was detectable in 3 patients by qPCR;18 months after the final treatment,Trypanosoma cruzi was detectable per qPCR in 4 of the 7 ***,between 12 and 18 months,7 participants of the 20 initial follow-up cases showed positive qPCR,indicating treatment ***:qPCR can be used as an alternative method for evaluating the effectiveness of the etiological treatment of CD,and can be applied to analyze early therapeutic *** study showed that benznidazole therapy had limited effectiveness in treating chronic CD patients,thus emphasizing the importance of conducting continued research for developing more effective therapies and diagnosis for CD.

关键词： Trypanosoma cruzi Chagas disease Antitrypanosomal therapy Benznidazole qPCR Therapeutic failure

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SARS-CoV-2 and hearing:An audiometric analysis of COVID-19 hospitalized patients

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Journal of Otology 2021年第3期16卷 158-164页

作者： Francisco Alves de Sousa Rodrigo Pinto costa sandra Xará Ana Nóbrega Pinto cecília Almeida e Sousa Centro Hospitalar Universitario do Porto Serviço de Otorrinolaringologia e Cirurgia da Cabeça e Pescoço(Otorhinolaryngology and Head&Neck surgery Department)Largo Prof.Abel Salazar4099-001PortoPortugal Unidade de Saúde Familiar Garcia de Orta ACeS Porto OcidentalMedicina Geral e Familiar(Family Medicine)R.Pinho Leal 294150-620PortoPortugal Centro Hospitalar Universitario do Porto Serviço de Doenças Infeciosas(Infectious Diseases Department)Largo Prof.Abel Salazar4099-001PortoPortugal

Purpose COVID-19 associated hearing loss is still an ongoing matter of *** original studies exist on audiological effects of SARS-CoV-2 infection in hospitalized *** main objective was to determine whether SARS-CoV-2 may affect auditory function in clinically ill COVID-19 *** and methods COVID-19 patients with moderate-severe disease and without prior history of hearing abnormalities were enrolled from a tertiary referral center,and matched with *** performed an audiometric evaluation,and thresholds were *** 120 ears from 60 patients were *** with COVID-19 showed worse mean auditory thresholds starting from 1000 Hz through higher frequencies,when compared to controls(1000 Hz:18.52±5.49 dB HL in controls vs 25.36±6.79 dB HL in COVID-19,p<0.001;2000Hz:17.50±5.57 dB HL in controls vs 21.96±7.05 dB HL in COVID-19,p=0.010;3000Hz:17.97±8.07 dB HL in controls vs 25±9.38 dB HL in COVID-19,p=0.003;4000 Hz:20.16±10.12 dB HL in controls vs 29.55±11.26 dB HL in COVID-19,p=0.001;8000 Hz:31.09±12.75 dB HL in controls vs 40.71±19.40 dB HL in COVID-19,p=0.030;Pure Tone Average:20.42±4.29 dB HL in controls vs 24.85±5.62 dB HL in COVID-19,p=0.001).Statistical significance persisted after adjusting for confounders such as age,gender and various comorbidities(p<0.05).Conclusions SARS-CoV-2 may affect hearing in COVID-19 patients with moderate-severe *** are in line with the previous suggested effects of COVID-19 on auditory *** study is expected to encourage further research on this topic.

关键词： SARS-CoV-2 COVID-19 Coronavirus Audiometry Hearing

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Parkinson's Disease:A Multisystem Disorder

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Neuroscience Bulletin 2023年第1期39卷 113-124页

作者： Helena Nunes costa Ana Raquel Esteves Nuno Empadinhas sandra Morais Cardoso CNC–Center for Neuroscience and Cell Biology and CIBB–Center for Innovative Biomedicine and Biotechnology University of Coimbra3004-504CoimbraPortugal IIIUC–Institute for Interdisciplinary Research University of Coimbra3004-504CoimbraPortugal Faculty of Medicine University of Coimbra3004-504CoimbraPortugal

The way sporadic Parkinson's disease(PD)is perceived has undergone drastic changes in recent *** a long time,PD was considered a brain disease characterized by motor disturbances;however,the identification of several risk factors and the hypothesis that PD has a gastrointestinal onset have shed additional ***,after recognition of prodromal non-motor symptoms and the pathological processes driving their evolution,there is a greater understanding of the involvement of other organ *** this reason,PD is increasingly seen as a multiorgan and multisystemic pathology that arises from the interaction of susceptible genetic factors with a challenging environment during aging-related decline.

关键词： Parkinson's disease Gut microbiome Intestinal barrier α-Synuclein Blood-brain barrier

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Auriculotherapy in primary health care: A large-scale educational experience in Brazil

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Journal of Integrative Medicine 2019年第4期17卷 302-309页

作者： Charles Dalcanale Tesser Ari Ojeda Ocampo More Melissa costa Santos Emiliana Domingues Cunha da Silva Fatima Terezinha Pelachini Farias Lucio Jose botelho Department of Public Health Federal University of Santa Catarina Floriandpolis Santa Catarina 88036-800 Brazil Post-graduate Program in Public Health Federal University of Santa Catarina Florianopolis Santa Catarina 88036-800 Brazil Integrative Medicine and Acupuncture Service University Hospital Federal University of Santa Catarina Floriandpolis Santa Catarina 88036-800 Brazil Floriandpolis Municipality Health Office Florianopolis Santa Catarina 88036-800 Brazil

Auriculotherapy consists of physical stimuli applied to the outer ear and is commonly associated with traditional Chinese medicine (TCM). The authors present and discuss the development of a course that offers a semi-on-site auriculotherapy course for Brazilian primary health care (PHC) professionals. The course was funded by the Brazilian Ministry of Health and developed at the Federal University of Santa Catarina in 2015 by a team of experts in auriculotherapy. It consisted of 75 h of distance learning (five sequential modules) and 5 h of on-site learning. The modules included the following items:(1) introduction to integrative practices;(2) ear reflexology;(3) introduction to TCM;(4) biomedical view of auriculotherapy;and (5) auriculotherapy in PHC. The teaching material in eluded a workbook for each module, 14 video lectures and an interactive ear(on line resource) to study location and application to the main auricular points. The on-site lectures follow a structured script of ear palpation techniques, auricular seed insertion practice and clinical case discussions, under the supervision of trained instructors. The course was offered in 2016 and 2017 and on-site lectures took place in 25 cities, covering all Brazilian regions, in coordination with municipal or state boards of health. A total of 4273 health professionals concluded the training and their evaluation of the course was highly positive. The Brazilian experience of large-scale training shows the potential to disseminate auriculotherapy in the context of PHC, given that its practice is fast, easy to learn, safe, effective for different health problems and well accepted by the patients.

关键词： Auriculotherapy Traditional Chinese medicine Primary health care Health education Distance learning

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Management of bone metastases in renal cell carcinoma: bone-targeted treatments, systemic therapies, and radiotherapy

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Journal of Cancer Metastasis and Treatment 2021年第1期7卷 628-639页

作者： AndréMansinho Priscila Nejo Tito Leitão sandra Casimiro Luís costa Serviço de Oncologia Médica Centro Hospitalar Universitário Lisboa NorteHospital de Santa MariaLisboa 1649-035Portugal Luis costa Laboratory Instituto de Medicina MolecularFaculdade de Medicina da Universidade de LisboaLisboa 1649-035Portugal Instituto Português de Oncologia Lisboa Francisco Gentil Lisboa 1649-035Portugal Urology Department Centro Hospitalar Universitário Lisboa NorteHospital de Santa MariaLisboa 1649-035Portugal

Metastatic renal cell carcinoma(mRCC)presents with bone metastases in around 20%-30%of patients enrolled in the most recent first-line clinical *** of several new agents in first line,in both monotherapy and combination,has significantly improved patient ***,the activity of such agents on bone metastases is unclear and management of these patients is complex,due to potential complications that can significantly impair quality of *** review addresses mRCC diagnosis and monitoring and summarizes the current evidence on systemic therapy,ablative therapies such as stereotactic ablative radiotherapy and surgery,and supportive therapy with bone-targeting agents for these patients,with the goal of improving their outcomes.

关键词： Bone metastases renal cell carcinoma bone targeted treatments radiotherapy systemic therapy

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Association between EGF +61A/G polymorphism and gastric cancer in Caucasians

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World Journal of Gastroenterology 2011年第4期17卷 488-492页

作者： Ana Paula Araújo Bruno M costa Ana L Pinto-Correia Maria Fragoso Paula Ferreira Mário Dinis-Ribeiro sandra costa Rui M Reis Rui Medeiros Molecular Oncology Group Portuguese Institute of Oncology of Porto Life and Health Sciences Research Institute (ICVS) School of Health Sciences Oncology Department Portuguese Institute of Oncology of Porto Gastroenterology Department Portuguese Institute of Oncology of Porto Portugal Cintesis/Faculty of Medicine University of Porto Centro de Estudos em Biomedicina Health Sciences Faculty-Fernando Pessoa University Instituto de Ciencias Biomédicas de Abel Salazar Abel Salazar Institute for the Biomedical Sciences

AIM: To investigate the association between epidermal growth factor (EGF) +61A/G polymorphism and susceptibility to gastric cancer, through a cross-sectional study. METHODS: Polymerase chain reaction resctriction fragment lenght polymorphism analyses were used to genotype EGF +61 in 207 patients with gastric lesions (162 patients with gastric adenocarcinomas, 45 with atrophy or intestinal metaplasia) and 984 controls. All subjects were Caucasian. RESULTS: Genotype distribution was 23.5% for GG and 76.5% for GA/AA in the control group, 18.4% for GG and 68.6% for GA/AA in the entire group with gastric lesions and 17.9% for GG and 82.1% for GA/AA in the group with gastric adenocarcinoma. No statistically significant associations were found between EGF +61 variants and risk for developing gastric cancer [odds ratios (OR) = 1.41, 95% confidence intervals (CI): 0.90-2.21, P = 0.116]. However, the stratification of individuals by gender revealed that males carrying A alleles (EGF +61A/G or AA) had an increased risk for developing gastric cancer as compared to GG homozygous males (OR = 1.55, 95% CI: 1.05-2.28, P = 0.021). CONCLUSION: In summary, we found that males who were A carriers for EGF +61 had an increased risk for developing gastric cancer. This result may be explained by the suggestion that women secrete less gastric acid than men.

关键词： Epidermal growth factor polymorphism Epidermal growth factor receptor Gastric cancer

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Methods and parameters of melting curve analysis for identification of Leishmania species:A scoping review

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Asian Pacific Journal of Tropical Medicine 2021年第12期14卷 528-542页

作者： Juliana J G Ferreira Fernanda S Nascimento Glaucia E B Marcon Eros A de Almeida sandra C B costa Laboratório de Diagnóstico de Doenças Infecciosas por Técnicas de Biologia Molecular Departamento de Clínica MédicaFaculdade de Ciências MédicasUniversidade Estadual de Campinas(UNICAMP)Campinas13083-887SPBrazil Fundação Oswaldo Cruz Mato Grosso do Sul(FIOCRUZ MS) Rua Gabriel Abrão92Jardim das NaçõesCampo Grande79081-746 MSBrazil Grupo de Estudos em Doença de Chagas Departamento de Clínica MédicaFaculdade de Ciências MédicasUniversidade Estadual de Campinas(UNICAMP)Campinas13083-887SPBrazil

Leishmaniasis is a set of diseases with a worldwide distribution that affects mainly economically underprivileged populations in developing countries. It has a major impact on public health, with a global cost of billions of dollars per year. The treatment and control of leishmaniasis vary according to the Leishmania species involved, which require reliable methods for species identification. Since most of the currently used methods have limitations, there is a need for assays that allow rapid, precise identification of the offending species. Real-time polymerase chain reactions in conjunction with dissociation curve analysis have been used to detect differences in the DNA composition of selected genes of Leishmania spp. Kinetoplast DNA is the main molecular target used because of its high copy number per parasite, but other targets have also been studied. As part of an effort to establish melting temperature standards for each target gene, we have reviewed the pertinent literature available in public databases, including Pub Med, Web of Science, Sci ELO and LILACS, using the keywords "Leishmania", "leishmaniasis", "realtime PCR", "melting temperature", and "melting curve", alone or in combination. After applying eligibility criteria, 27 articles were selected for analysis. A considerable variation in the methodologies analyzed was found regarding molecular targets, standardization of the methods, reproducibility and specificity. Because of this, statistical analysis was not performed. In most cases, the methods were able to differentiate the parasite at the subgenus level or few species regardless of the target chosen.

关键词： Leishmaniasis Melting temperature Real-time PCR

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Short-term complementary and alternative medicine on quality of life in women with fibromyalgia

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Journal of Integrative Medicine 2016年第1期14卷 29-35页

作者： Paulo Araujo Dias Andre Brito Bastos Guimaraes Andrea de Oliveira Albuquerque Karoline Lucas de Oliveira Maria Luzete costa Cavalcante Sergio botelho Guimaraes Faculty of Medicine Department of SurgeryFederal University of Ceara60430-140FortalezaBrazil Faculty of Pharmacy Dentistry and NursingFederal University of Ceara60430-160FortalezaBrazil

BACKGROUND： Fibromyalgia （FMS） is a syndrome characterized by chronic widespread musculoskeletal pain, whose etiology is not completely understood. Different therapeutic approaches have been used with inconsistent results. This observation does not invalidate the continued search for alternative treatments aimed at improving quality of life （QoL） in FMS. OBJECTIVE： This study compared three classica acupuncture （AC）, electroacupuncture （EAC） and promotion of QoL in FMS patients. traditional Chinese medicine （TCM） therapies： moxibustion （MX） in the management of pain and DESIGN, SETTING, PARTICIPANTS AND INTERVENTIONS： A preliminary, group-assigned, comparative study enrolled 30 women, mean age （46.90_＋9.24） years （range 20-60 years）, who met the 1990 American College of Rheumatology criteria for FMS diagnosis and a pain-pressure threshold （PPT）〈 4 kg/cm2. The study was conducted in a teaching tertiary-care medical institution from May 2010 through April 2012. AC, EAC and MX were delivered for 30 min, once a week, for 8 weeks, bilaterally at Neiguan （PC6）, Hegu （LI4）, Yanglingquan （GB34）, Sanyinjiao （SP6） and Taichong （LR3） acupoints. MAIN OUTCOME MEASURES： Each week, immediately before treatment and after treatment, subjects were tested for PPTs, Wong-Baker Faces Pain Scale （WBFPS; for pain intensity） and Medical Outcomes Study 36-item Short Form Health Survey （SF-36： for QoL）. RESULTS： There was no significant improvement in pain or reduction of tender points in any of the groups studied, at the end of the 8th session. Significant improvement of QoL was perceived in vitality （after AC treatment） and in mental health （after EAC and MX treatments）. CONCLUSION： TCM therapies （AC, EAC and MX） promoted an improvement in the QoL in two areas （vitality and mental health） in FMS women. Further large-scale clinical trials are required to confirm this effect.

关键词： quality of life medicine, Chinese traditional acupuncture therapy women

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Fluid-dynamics evaluation in a conical spouted bed and characterization of foxtail millet seeds

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Particuology 2015年第6期13卷 75-81页

作者： Lidiane Diniz do.Nascimento Lorena Gomes Corumba sandra Cristina dos Santos Rocha Osvaldir Pereira Taranto Cristiane Maria Leal costa Lenio Jose Guerreiro de Faria Federal University of Pard Faculty of Chemical Engineering Laboratory of Drying and Particle Coating Augusto Correa Street w/n 66075-110 BelemPA Brazil University of Campinas School of Chemical Engineering. Albert Einstein Avenue 500 13083-852 Campinas $8o Paulo Brazil

Foxtail millet （Setaria italica） is one of the most valuable species in economic terms in the genus Setaria and plays an important role in human nutrition, animal feed, and agriculture. The present study described chemical, physical, and quality aspects of seeds of foxtail millet. Furthermore, the fluid-dynamic behavior of the seeds was evaluated in a conical spouted bed, which has advantages in terms of promoting the cyclic and regular movement of the seed particles. Dynamic parameters of spouting （minimum spouting veloc- ity, stable and peak pressure drop） were determined and compared with those obtained from empirical correlations available in the literature. The results obtained from physical characterization showed that the seeds can be classified as belonging to Group D of Geldart, having a non-rough surface, mean diameter of 1.75 mm, and sphericity of 0.74. Fluid-dynamics analysis showed that the seeds are suitable for processing in a spouted bed, which is in agreement with the results of particle physical characterization.

关键词： Foxtail millet Parameters of spouting Characterization Conical spouted bed

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Cytomegalovirus frequency in neonatal intrahepatic cholestasis determined by serology, histology, immunohistochemistry and PCR

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World Journal of Gastroenterology 2009年第27期15卷 3411-3416页

作者： Maria Angela Bellomo-Brandao Paula D Andrade sandra CB costa cecilia AF Escanhoela Jose Vassallo Gilda Porta Adriana MA De Tommaso Gabriel Hessel Department of Pediatrics Faculty of Medical Sciences State University of Campinas (UNICAMP)Campinas-SP 13083-970 Brazil Department of Internal Medicine Faculty of Medical Sciences State University of Campinas (UNICAMP)Campinas-SP 13083-970 Brazil Department of Pathology Faculty of Medical Sciences State University of Campinas (UNICAMP) Campinas-SP 13083-970 Brazil Department of Pediatrics Children's Institute of Medical School of the University of So Paulo (USP) So Paulo-SP 05403-000 Brazil

AIM: To determine cytomegalovirus (CMV) frequency in neonatal intrahepatic cholestasis by serology, histological revision (searching for cytomegalic cells), immunohistochemistry, and polymerase chain reaction (PCR), and to verify the relationships among these ***: The study comprised 101 non-consecutive infants submitted for hepatic biopsy between March 1982 and December 2005. Serological results were obtained from the patient's f iles and the other methods were performed on paraffin-embedded liver samples from hepatic biopsies. The following statistical measures were calculated: frequency, sensibility, specif ic positive predictive value, negative predictive value, and ***: The frequencies of positive results were as follows: serology, 7/64 (11%); histological revision, 0/84; immunohistochemistry, 1/44 (2%), and PCR, 6/77 (8%). Only one patient had positive immunohistochemical fi ndings and a positive PCR. The following statistical measures were calculated between PCR and serology: sensitivity, 33.3%; specificity, 88.89%; positive predictive value, 28.57%; negative predictive value, 90.91%; and accuracy, 82.35%. CONCLUSION: The frequency of positive CMV varied among the tests. Serology presented the highest positive frequency. When compared to PCR, the sensitivity and positive predictive value of serology were low.

关键词： Cytomegalovirus Hepatitis Neonatal Cholestasis Liver Children Immunohistochemistry Polymerase chain reaction

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