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Xiao Tan san Jie Fang Hampers the Growth of Colon Cancer Stem Cells through the Wnt/β-catenin Signaling Pathway

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World Journal of Traditional Chinese Medicine 2024年第2期10卷 171-179页

作者： yu-Qi Zhou Jun-Fei yuan Hong-Shi Shen Ya-Ping Wang Han-Fei Shi De-Jian Pan Min Ye Department of Hematology and Oncology The 904^(th) Hospital of PLA Joint Logistic Support ForceWuxiJiangsuChina Department of Clinical Laboratory The 904^(th)Hospital of PLA Joint Logistic Support ForceWuxiJiangsuChina Department of Traditional Chinese Medicine Second Affiliated Hospital of Naval Medical UniversityShanghaiChina

Objective: The purpose of this research was to examine the potential anticancer properties of Xiao Tan san Jie Fang(XTSJF) and its potential mechanism of action against colorectal cancer. Materials and Methods: HCT116 cells were induced into HCT116 spheres in DMEM/F12 medium by treatment with epidermal growth factor + fibroblast growth factor + leukemia inhibitory factor + B27. The proliferation ability and stemness of HCT116 spheres was examined. Various concentrations of XTSJF were used to treat HCT116 spheres to observe the impact on proliferation, apoptosis, and expression of stem cell markers. Next, Wnt/β-catenin pathway-related factor proteins were detected. Results: The findings revealed that XTSJF suppressed cell growth and induced cell death in HCT116 cells in a dosage-dependent manner. Similarly, XTSJF promotes apoptosis, inhibits cell proliferation, prolongs survival, and maintains the expression of stem cells through the Wnt/-catenin/TCF4 axis. XTSJF also inhibits AKT activity and subsequently activates glycogen synthesis kinase-3β expression, inhibiting Wnt/beta-catenin pathway activity and downstream target gene transcript expression. The Wnt/β-catenin signaling pathway is inhibited by the XTSJF, leading to the suppression of colon cancer stem cell proliferation. Conclusion: Xiaotan sanjie Prescription inhibited colon cancer stem cell growth through Wnt/β-catenin signaling pathway.

关键词： Apoptosis colorectal cancer proliferation traditional Chinese medicine Wnt/β-catenin signaling

Nonadiabatic Geometric Quantum Computation with Asymmetric Superconducting Quantum Interference Device

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Communications in Theoretical Physics 2002年第9期37卷 285-291页

作者： HAO san-Ru HOU Bo-yu XI Xiao-Qiang yuE Rui-Hong Institute of Modern Physics Northwest University Xi''an 710069 China Galculating Physics Division Department of Computer Teaching Hunan Normal University Changsha 410081 China Institute of Modern Physics Northwest University Xi''an 710069 China Institute of Modern Physics Northwest University Xi''an 710069 China Institute of Modern Physics Northwest University Xi''an 710069 China

We propose a method of controlling the dc-SQUID (superconducting quantum interference device) system by changing the gate voltages, which controls the amplitude of the fictitious magnetic fields B-z, and the externally applied current that produces the piercing magnetic flux Phi(x) for the dc-SQUID system. We have also introduced a physical model for the dc-SQUID system. Using this physical model, one can obtain the non-adiabatic geometric phase gate for the single qubit and the non-adiabatic conditional geometric phase gate (controlled NOT gate) for the two qubits. It is shown that when the gate voltage and the externally applied current of the dc-SQUID system satisfies an appropriate constraint condition, the charge state evolution can be controlled exactly on a dynamic phase free path. The non-adiabatic evolution of the charge states is given as well.

关键词： non-adiabatic geometric phase gate dc-SQUID quantum computation

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不同贮藏温度对南美白对虾品质的影响

粮食与油脂 2024年第2期37卷 85-89页

作者：陈钰庞文媛周三女马腾飞吴先辉庞杰宁德职业技术学院福建福安355000 福建农林大学食品科学学院福建福州350002

以南美白对虾为研究对象,对比分析冷藏(4℃)、微冻(-2℃)和冻藏(-18℃)条件下,虾肉的品质变化。结果表明:随着贮藏时间的延长,不同贮藏温度条件下南美白对虾的保水性、蛋白质含量、肌原纤维蛋白活性、脂肪含量均不断降低,ATP关联化合物(... 详细信息

以南美白对虾为研究对象,对比分析冷藏(4℃)、微冻(-2℃)和冻藏(-18℃)条件下,虾肉的品质变化。结果表明:随着贮藏时间的延长,不同贮藏温度条件下南美白对虾的保水性、蛋白质含量、肌原纤维蛋白活性、脂肪含量均不断降低,ATP关联化合物(K值)、硫代巴比妥酸(TBA)值均呈上升趋势。南美白对虾在4、-2、-18℃条件下贮藏的货架期分别为6、20、50 d。

关键词：南美白对虾贮藏温度货架期

In vitro Degradation and Biocompatibility of Ca-P Coated Magnesium Alloy

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Chemical Research in Chinese Universities 2013年第2期29卷 285-289页

作者： XIAO Xing ZHU Qing-san SU Ying-chao LI Guang-yu The First Hospital of Jilin University Changchun 130021 P. R. China College of Materials Science and Engineering Jilin University Changchun 130022 P. R. China

Calcium-phosphate compounds（Ca-P） coating was prepared on an Mg-A1 alloy（AZ60）. Biodegradation of Ca-P coated magnesium alloy was evaluated in simulated body fluid（SBF） by examining the changes in magnesium ion concentration and pH value, which indicated that the Ca-P coating on magnesium alloy strongly affected the cor- rosion of magnesium alloy. Osteoblast MC3T3-E1 cells were utilized to investigate the cellular eytocompatibility. The cytocompatibility was measured by carrying out a series of tests, such as cholecystokinin-octapeptide（CCK-8） test, alkaline phosphatase activity（ALP） test, cellular morphology of hematoxylin-eosin（HE） staining and the induc- tion of apoptosis. It was found that the cell function showed better in the Ca-P coated Mg-alloy extract than in the uncoated magnesium alloy extract. In summary, the results indicate that the Ca-P coating can improve the corrosion resistance of magnesium alloy and elevate cellular proliferation and differentiation of osteoblast MC3T3-E1 cells.

关键词： Ca-P coating Magnesium alloy In vitro

Optimal Conclusive Teleportation of an Arbitrary d-Dimensional N-Particle Unknown State via a Partially Entangled Quantum Channel

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Communications in Theoretical Physics 2003年第2期39卷 157-166页

作者： HAO san-Ru HOU Bo-yu XI Xiao-Qiang yuE Rui-Hong Calculating Physics Division Department of Computer Teaching Hunan Normal University Changsha 410081 China Physikalisches Institut Philosphenweg 12 69120 Heidelberg Germany

In the paper we generalize the standard teleportation to the conclusive teleportation case which can teleportan arbitrary d-dimensional N-particle unknown state via the partially entangled quantum channel. We show that onlyif the quantum channel satisfies a constraint condition can the most general d-dimensional N-particle unknown state beperfect conclusively teleported. We also present a method for optimal conclusively teleportation of the N-particle statesand for constructing the joint POVM which can discern the quantum states on the sender's (Alice's) side. Two typicalexamples are given so that one can see how our method works.

关键词： N-粒子态量子隧道光学远距传物

淫羊藿苷对抑郁症模型小胶质细胞表型转化的调控作用

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天然产物研究与开发 2024年第2期36卷 187-195页

作者：曹利华高松王笑雨王真真贺红娟李娜白明苗明三河南中医药大学中医药科学院郑州450046 仲景宛西制药股份有限公司河南省中药固体制剂技术创新中心西峡474550 河南中医药大学药学院郑州450046

探索淫羊藿苷抗抑郁的作用机制,将SPF级KM小鼠分为空白组、模型组、盐酸氟西汀组(10 mg/kg)、淫羊藿苷高(50 mg/kg)、低(25 mg/kg)剂量组。除空白组外,其余各组小鼠采用56 d慢性不可预知温和应激建立抑郁症模型。造模第1 d开始灌胃给予... 详细信息

探索淫羊藿苷抗抑郁的作用机制,将SPF级KM小鼠分为空白组、模型组、盐酸氟西汀组(10 mg/kg)、淫羊藿苷高(50 mg/kg)、低(25 mg/kg)剂量组。除空白组外,其余各组小鼠采用56 d慢性不可预知温和应激建立抑郁症模型。造模第1 d开始灌胃给予各组小鼠相应药物,连续56 d。于给药后第53~56 d,进行行为学测试。末次给药后,取材。酶联免疫吸附剂实验(enzyme linked immunosorbent assay,ELISA)检测脑组织匀浆白细胞介素6(interleukin-6,IL-6)、白细胞介素10(interleukin-10,IL-10)、五羟色胺(5-hydroxytryptamine,5-HT)、多巴胺(dopamine,DA)、去甲肾上腺素(norepinephrine,NE)水平;实时荧光定量PCR(quantitative real-time PCR,RT-PCR)检测脑组织中IL-6、IL-10、诱导性一氧化氮合酶(inducible nitric oxide synthase,iNOS)、分化簇206(cluster of differentiation 206,CD206)mRNA表达;蛋白免疫印迹(Western blot)检测脑组织iNOS、CD206蛋白表达。体外培养小鼠小胶质细胞(BV-2),采用细胞增殖与毒性检测试剂盒(Cell Counting Kit-8,CCK-8)法检测淫羊藿苷的细胞毒性。BV-2细胞暴露于100μg/mL脂多糖及15μg/mL、25μg/mL的淫羊藿苷24 h后,收集细胞。免疫荧光技术检测细胞iNOS、CD206蛋白表达。研究结果表明,慢性不可预知温和应激致抑郁症模型制备成功,淫羊藿苷可降低抑郁症小鼠脑组织IL-6水平及IL-6、iNOS mRNA表达,升高脑组织IL-10、5-HT、DA、NE水平及IL-10、CD206 mRNA表达;降低脑组织iNOS蛋白,升高CD206蛋白表达;改善模型小鼠抑郁症样行为。淫羊藿苷可降低LPS刺激的BV-2细胞iNOS蛋白表达,升高CD206蛋白表达。结果表明,淫羊藿苷抑制小胶质细胞M1表型转化,促进M2表型转化,从而改善模型小鼠抑郁样行为。

关键词：淫羊藿苷抑郁症小胶质细胞表型转化细胞炎症模型

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移动荷载作用下建筑垃圾再生路面响应分析

公路 2024年第1期69卷 58-65页

作者：杨振宇杨三强褚嘉怡候月军刘杰河北大学建筑工程学院保定市071000 北京市政建设集团有限责任公司北京市100000 保定市绿生环境科技有限公司保定市071000

为研究砖混类建筑垃圾用于道路基层填筑的再生路面在移动荷载下的力学响应问题,通过室内试验对不同再生骨料掺量的再生混合料进行抗压回弹模量试验,分析再生混合料力学性能特点;利用ABAQUS有限元分析软件建立再生路面三维道路模型,分析... 详细信息

为研究砖混类建筑垃圾用于道路基层填筑的再生路面在移动荷载下的力学响应问题,通过室内试验对不同再生骨料掺量的再生混合料进行抗压回弹模量试验,分析再生混合料力学性能特点;利用ABAQUS有限元分析软件建立再生路面三维道路模型,分析了移动荷载下再生路面力学响应规律;并基于正交试验,就再生下基层厚度、底基层厚度及再生骨料掺量对结构力学响应影响进行敏感性分析。研究结果表明:(1)建筑垃圾再生骨料具有压碎值大、密度小、吸水率大的特点,再生混合料最佳含水率与最大干密度随再生料掺量的增多分别增大与减小,再生混合料抗压回弹模量随再生料掺量的增多呈现先增大后减小的趋势,因此将砖混类再生骨料用于基层铺筑时建议掺量在40%以下,并应严格控制再生骨料中砖混比例;(2)在移动荷载作用下,建筑垃圾再生路面动力响应具有“应变集中”与“应变交替”的现象,最大竖向应变达到381.94με;(3)建筑垃圾再生路面下基层厚度、底基层厚度对道路性能的影响大于再生骨料掺量的影响。研究结果可为建筑垃圾再生路面结构设计提供理论依据和技术参考。

关键词：道路工程路面基层建筑垃圾移动荷载力学响应正交试验

基于数据挖掘、网络药理学及实验验证探讨中药治疗变应性鼻炎的用药规律及相关药理分析

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天然产物研究与开发 2024年第1期36卷 137-154页

作者：王笑雨王召曹利华贺红娟王真真李秀敏苗明三河南中医药大学中医药科学院郑州450046 纽约医学院微生物和免疫学科纽约10595

基于中国知网、万方、维普数据库,以“中药+变应性鼻炎”“中药+过敏性鼻炎”“中药+鼻鼽”为主题,选取2000年1月至2022年2月公开发表的期刊文献,按照纳入与排除标准筛选临床治疗AR的中药方剂,统计高频药物的用药频次、剂量、药味、药... 详细信息

基于中国知网、万方、维普数据库,以“中药+变应性鼻炎”“中药+过敏性鼻炎”“中药+鼻鼽”为主题,选取2000年1月至2022年2月公开发表的期刊文献,按照纳入与排除标准筛选临床治疗AR的中药方剂,统计高频药物的用药频次、剂量、药味、药性、归经、功效等,并进行关联规则和系统聚类分析,共筛选符合标准的中药方剂有252首,共223味中药,其中用药频次≥30的高频中药有21种,以解表药和补虚药为主,药性以辛温为主,关联规则挖出21对频次≥64的药物组合,其中黄芪-辛夷药对出现频次最高。利用网络药理学对黄芪-辛夷药对的活性成分、靶点蛋白和通路等进行分析,结果显示黄芪-辛夷药对共有48个有效成分,对应的潜在靶点共306个,包括PTGS2、HSP90、CALM1、NOS2、CHRM1等;GO富集和KEGG通路分析显示核心靶点参与对外源性刺激的反应、氧化还原酶活性等多项进程,并通过神经活性配体-受体相互作用通路、钙离子信号通道等通路作用于AR,分子对接结果显示黄芪-辛夷可能通过细辛素、芒柄花素、山柰酚、槲皮素作用于PTGS2、CHRM1、CYP1B1等靶点发挥治疗AR的作用。基于上述结论进行体内实验验证,结果证实黄芪-辛夷药对能够有效降低AR小鼠血清、肺泡灌洗液以及鼻腔灌洗液中组胺、总IgE、RW特异性IgE、TNF-α和IL-6等炎性因子的水平,升高抗炎因子IL-10的水平,抑制炎症反应的发展,显著改善AR小鼠挠鼻和喷嚏症状,并降低肺组织中PTGS2、HSP90、NOS2蛋白的表达水平。因此,本研究表明黄芪-辛夷药对可能是通过抑制HSP90/PTGS2/NOS2通路进而抑制炎症反应、缓解AR小鼠的症状从而发挥治疗AR的作用。

关键词：变应性鼻炎用药规律关联规则网络药理学分子对接体内实验验证

Physicochemical Properties and Evaluation of Microemulsion Systems for Transdermal Delivery of Meloxicam

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Chemical Research in Chinese Universities 2007年第1期23卷 81-86页

作者： yuAN yue LI san-ruing yu Li-min DENG Pan ZHONG Da-fang School of Pharmacy Shenyang Pharmaceutical University Shenyang 110016 P. R. China Center for Drug Metabolism and Pharmacokinetics Research Shanghai Institute of Materia Medica Chinese Academy of Sciences Shanghai 201203 P. R. China

Microemulsion systems, composed of water, isopropyl myristate （IPM）, polyoxyethylene sorbitan trioleate （Tween 85 ）, and ethanol, were investigated as transdermal drug delivery vehicles for a lipophilic model drug（ meloxicam）. The purpose of this study was to investigate the physicochemieal properties of the tested microemulsion and to find the correlation between the physicoehemical properties and the skin permeation rate of the microemulsion. Pseudo-ternary phase diagram of the investigated system at a constant surfactant/cosurfactant mass ratio （ Km = 1 ： 1 ） was constructed by titration at 20℃, and the five fommlations were selected for further research in the o/w microemulsion domains. The values of electrical conductivity and viscosity showed that the selected systems were bicontinuous or non-spherical o/w microemulsion, and the electrical conductivity and viscosity were increased with increasing the content of water. These results suggest that the optimum formulation of microemulsion, containing 0. 375 meloxicam, 5% isopropyl myristate, 25% Tween 85. 25% ethanol, and water, showed the maximum permeation rate. It had a high electrical conductivity, small droplet size, and proper viscocity.

关键词： Microemulsion Physicochemical property Transdermal delivery Meloxicam Polyoxyethylene sorbitan triolcate