检索结果-南通市图书馆

High basal defense gene expression determines sorghum resistance to the whorl-feeding insect southwestern corn borer

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Insect Science 2013年第3期20卷 307-317页

作者： Wei-Ning Cheng Jia-Xin lei William l. Rooney Tong-Xian liu keyan Zhu-salzman key laboratory of Integrated Pest Management on Crops in Northwestern loess Plateau Ministry of Agriculture Northwest A&F Uni- versity Yangling Shaanxi China Department of Entomology Department of Soil and Crop Sciences Vegetable & Fruit Improvement Center Texas A&M University College Station Texas United States

Southwestern corn borer （SWCB, Diatraea grandiosella） and fall armyworm （FAW, Spodopterafrugiperda） are major pests of sorghum in the southern United States. Host plant resistance is a desirable means for reducing plant damage and yield losses from both insects. In this study, we evaluated 12 sorghum lines for whorl-stage resistance to leaf-feeding SWCB and FAW in greenhouse and laboratory bioassays. Differential plant responses were detected against the two insects. Among 12 lines tested, CM1821, Della and PI196583 were resistant to both insects, while BTx2752 was largely susceptible. line R.09110 was resistant to SWCB, but susceptible to FAW, whereas Redbine-60 was suscep- tible to SWCB, but not to FAW. In addition, we quantified various chemical components in the plants and determined their association with insect resistance. Tannin and chloro- phyll in leaves did not show any significant correlation with resistance to either insects, but contents of soluble protein in general were negatively correlated with resistance to both insects. Endogenous soluble sugar and dhurrin were only positively correlated with resistance to SWCB, but not with FAW resistance. To gain some molecular insight into resistance mechanism of sorghum to SWCB, we performed qPCR reactions for key genes encoding enzymes involved in dhurrin and jasmonic acid （JA） biosynthesis on selected resistant or susceptible lines. Although these genes were rapidly and strongly induced by insect feeding in all lines, the observed resistance is lik.l. explained by higher constitutive dhurrin contents in some resistant lines and higher basal JA biosynthesis in others. Our results suggest that sorghum utilizes multiple strategies to defend itself against SWCB.

关键词： Diatraea grandiosella host plant resistance resistance mechanism sorghum,Spodoptera frugiperda

Suppressing tawny crazy ant (Nylanderia fulva) by RNAi technology

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Insect Science 2020年第1期27卷 113-121页

作者： Jia Meng Jiaxin lei Andrew Davitt Jocelyn RHolt Jian Huang Roger Gold Edward l Vargo Aaron MTarone keyan Zhu-salzman College of Plant Protection Fujian Agricultural and Forestry UniversityFuzhouFujianChina Department of Entomology Texas A&M UniversityCollege StationTexasUSA Institute for Plant Genomics&Biotechnology Texas A&M UniversityCollege StationTexasUSA

A bstract The tawny crazy ant(Nylanderia fulva)is a new invasive pest in the United *** present,its management mainly relies on the use of synthetic insecticides,which are generally ineffective at producing lasting control of the pest,necessitating alternative environmentally friendly *** this study,we evaluated the feasibility of gene silencing to control this ant *** housekeeping genes encoding actin(NfActin),coatomer subunit β (NfCOPP),arginine kinase(NfArgk),and V-type proton ATPase subunits A(NfvATPaseA),B(NfvATPaseB)and E(NfvATPaseE)were *** analysis revealed high sequence similarity to homologs from other ant species,particularly the Florida carpenter ant(Camponotus floridanus).To silence these genes,vector l4440 was used to generate six specific RNAi constructs for bacterial ***-inactivated,dsRNA-expressing Escherichia coli were incorporated into artificial *** ants exhibited reduced endogenous gene expression after feeding on such diet for 9 ***,only ingestion of dsRNAs of NfCOPfi(a gene involved in protein trafficking)and NfArgk(a cellular energy reserve regulatory gene in invertebrates)caused modest but significantly higher ant mortality than the *** results suggest that bacterially expressed dsRNA can be orally delivered to ant cells as a mean to target its *** efficacy is necessary for the RNAi-based approach to be useful in tawny crazy ant management.

关键词： artificial diet gene silencing housekeeping gene Nylanderia fulva RNAi survival rate

Deleterious effects of electron beam irradiation on development and reproduction of tomato/potato psyllids, Bactericera cockerelli

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Insect Science 2020年第6期27卷 1311-1321页

作者： Jiaxin lei Jia Meng Ivy WChen Weining Cheng Andrea l.Beam Md-Sajedul Islam Woodward D.Bailey Suresh Pillai keyan Zhu-salzman Department of Entomology Texas A&M UniversityCollege StationTXUSA Institute for Plant Genomics&Biotechnology Texas A&M UniversityCollege StationTXUSA key laboratory of Integrated Pest Management on Crops in Northwestern loess Plateau Ministry of AgricultureNorthwest A&F UniversityYanglingShaanxiChina USDA-APHIS-PPQ-S&T-CPHST MiamiFLUSA National Center for Electron Beam Research Texas A&M UniversityCollege StationTXUSA

The potato/tomato psyllid Bactericera cockerelli causes serious damage to several solanaceous crops by direct feeding and vectoring Candidatus liberibacter solanacearum,a bacterial *** beam(eBeam)irradiation is an environmentally friendly,chemical-free alternative method that is increasing in use for disinfestation of insect *** hypothesize that this irradiation technology will have detrimental effects on potato psyllid and thus impede its disease *** this end,we explored the effects of eBeam treatment ranging from 50 to 500 Gy on survival,development and reproduction of this *** on psyllids was apparently *** irradiated at 350 Gy,eggs could not hatch,1st instar nymphs failed to emerge,and although a small portion of irradiated 5th instar nymphs survived,the emerged adults were mostly *** in eclosed adults suggests harmful effects of eBeam on *** was seriously impaired when female psyllids were exposed to eBeam at the 5th instar nymphal or young adult stage,presumably due to inability to form *** addition,reciprocal crosses between irradiated and untreated psyllids indicated that female psyllids were more radiosensitive than males to *** together,these findings indicate that eBeam negatively impacted potato psyllid development and reproduction,which would inevitably compromise its disease transmission capacity.A dose of 350 Gy can be considered as a reference dose for effective control of potato psyllids.

关键词： Bactericera cockerelli development electron beam(eBeam) irradiation reproductive system

Molecular and functional characterization of three odorant-binding proteins from the wheat blossom midge,Sitodiplosis mosellana

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Insect Science 2020年第4期27卷 721-734页

作者： Wei-Ning Cheng Yu-Dong Zhang Wei liu Guang-Wei li keyan Zhu-salzman key laboratory of Integrated Pest Management on Crops in Northwestern loess Plateau Ministry of AgricultureNorthwest A&F UniversityYang lingShaanxiChina Shaanxi key laboratory of Chinese Jujube Van'an UniversityVan'anShaanxiChina Department of Entomology Texas A&M UniversityCollege StationTXUSA

Sitodiplosis mosellana,a periodic but devastating wheat pest,relies on wheat spike volatiles as a cue in sclecing hosts for *** odorant-binding proteins(OBPs)are thought to play essential roles in filtering,binding and transporting hydropho-bic odorant molecules to specific *** date,the molecular mechanisms underlying *** olfaction are poorly ***,three *** antenna-specific OBP genes,SmosOBPII,16 and 21,were cloned and bacterially *** properties of the recombinant proteins to 28 volatiles emitted from wheat spikes were in-vestigated using fluorescence competitive binding *** analysis suggested that these SmosOBPs belong to the Classic OBP ***-binding analysis showed that all three SmosOBPs preferentially bound alcohol,ester and ketone com-pounds,and SmosOBP11 and 16 also selectively bound terpenoid *** par-ticular,the three SmosOBPs had high binding affinities(kil/l)to 3-hexanol and cis-3-hexenylacetate that elicited strong electroantennogram(EAG)response fromfemale *** addition,SmosOBP11 displayed significantly higher binding(kil)than SmosOBP16 and 21 to l-octen-3-ol,D-panthenol,a-pinene and heptyl acetate which elicited significant EAG response,suggesting that SmosOBP11 plays a ma-jor role in recognition and transportation of these *** findings have provided important insight into the molecular mechanism by which *** specifically rec-ognizes plant volatiles for host selection,and have facilitated identification of effective volatile attractants that are potentially useful for pest monitoring and trapping.

关键词： binding assay electroantennogram response host selection odorant-binding protein Sitodiplosis mosellana wheat spike violates

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

内毒素性亚硝基减少症

德国医学 1999年第4期16卷 196-197页

作者： A.l.salzman 李树生 Division of Critical Care Medicine Children's Hospital Medical CenterCincinnatiOH 45229USA 同济医科大学附属同济医院

近年来,在败血症性休克中,一氧化氮越来越被重视。实验模型与临床实践均已证实,一氧化氮是生理功能的重要调节剂,也是引起器官衰竭的恶化性组织毒素与介质。 Gunderson等发现,在发生内毒素性休克的开始3小时内,应用硝普钠可以部分地防... 详细信息

近年来,在败血症性休克中,一氧化氮越来越被重视。实验模型与临床实践均已证实,一氧化氮是生理功能的重要调节剂,也是引起器官衰竭的恶化性组织毒素与介质。 Gunderson等发现,在发生内毒素性休克的开始3小时内,应用硝普钠可以部分地防止肝窦和窦后血流的减少,并且还可以大大减少白细胞在这两处的粘附。但硝普钠对胆汁的流动、血清转氨酶的升高和白细胞的迁移并无影响。

关键词：内毒素休克亚硝基减少 iNOS 一氧化氮

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

采用广义HOUGH变换的图象匹配(英文)

通信学报 1986年第6期 38-47页

作者： l.S.戴维斯,l.基青,胡复培,黄尚硕美国马里兰大学美国马里兰大学美国马里兰大学美国马里兰大学

本文描述一种符号匹配技术;它基于采用高阶图象特征,例如团滴和条带。

关键词： HOUGH line 广义