检索结果-南通市图书馆

In vitro comparison of human plasma-based and self-assembled tissue-engineered skin substitutes:two different manufacturing processes for the treatment of deep and difficult to heal injuries

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Burns & Trauma 2023年第1期11卷 800-813页

作者： Álvaro Sierra-Sánchez Brice Magne Etienne Savard Christian Martel Karel Ferland Martin A.Barbier Anabelle Demers Danielle Larouche salvador arias-santiago Lucie Germain LOEX Tissue Engineering Laboratory and Department of Surgery Faculty of MedicineUniversitéLaval140118e rueQuébec(Québec)G1J 1Z4Canada CHU de Québec-UniversitéLaval Research Center Division of Regenerative Medicine140118e rueQuébec(Québec)G1J 1Z4Canada Unidad de Producción Celular e Ingeniería Tisular(UPCIT) Virgen de las Nieves University Hospitalibs.GRANADAAndalusian Network for the design and translation of Advanced TherapiesAv.de las Fuerzas ArmadasNº24ªPlanta Ed.de Gobierno18014GranadaSpain Department of Dermatology Virgen de las Nieves University HospitalAv.MadridNº11-1518012GranadaSpain Department of Dermatology Faculty of MedicineUniversity of GranadaAv.de la InvestigaciónNº1118016GranadaSpain

Background:The aim of this in vitro study was to compare side-by-side two models of human bilayered tissue-engineered skin substitutes(hbTESSs)designed for the treatment of severely burned *** are the scaffold-free self-assembled skin substitute(SASS)and the human plasma-based skin substitute(HPSS).Methods:Fibroblasts and keratinocytes from three humans were extracted from skin biopsies(N=3)and cells from the same donor were used to produce both hbTESS *** SASS manu-facture,keratinocytes were seeded over three self-assembled dermal sheets comprising fibroblasts and the extracellular matrix they produced(n=12),while for HPSS production,keratinocytes were cultured over hydrogels composed of fibroblasts embedded in either plasma as unique biomaterial(Fibrin),plasma combined with hyaluronic acid(Fibrin-HA)or plasma combined with collagen(Fibrin-Col)(n/biomaterial=9).The production time was 46-55 days for SASSs and 32-39 days for *** were characterized by histology,mechanical testing,PrestoBlue™-assay,immunofluorescence(Ki67,Keratin(K)10,K15,K19,Loricrin,type IV collagen)and Western blot(type I and IV collagens).Results:The SASSs were more resistant to tensile forces(p-value<0.01)but less elastic(p-value<0.001)compared to HPSSs.A higher number of proliferative Ki67+cells were found in SASSs although their metabolic activity was *** epidermal differentiation,no significant difference was observed in the expression of K10,K15,K19 and ***,the production of type I and type IV collagens and the adhesive strength of the dermal-epidermal junction was higher in ***:This study demonstrates,for the first time,that both hbTESS models present similar in vitro biological ***,mechanical properties differ and future in vivo experiments will aim to compare their wound healing potential.

关键词： Biomaterial Burn Fibrin Human plasma Self-assembly Skin substitute Tissue engineering

Histopathological impact of SARS-CoV-2 on the liver:Cellular damage and long-term complications

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

World Journal of Gastroenterology 2024年第22期30卷 2866-2880页

作者： Alfonso Rodriguez-Espada Moises Salgado-de la Mora Briana Mariette Rodriguez-Paniagua Nathaly Limonde la Rosa Monica Itzel Martinez-Gutierrez santiago Pastrana-Brandes Nalu Navarro-Alvarez Department of Molecular Biology Universidad Panamericana School of MedicineCampus MéxicoMexico 03920Mexico Department of Internal Medicine Instituto Nacional de Ciencias Médicas y Nutrición Salvador ZubiránMexico 14080Mexico Department of Surgery University of Colorado Anschutz Medical CampusDenverCO 80045United States PECEM Facultad de MedicinaUniversidad Nacional Autónoma de MéxicoMexico 04360Mexico Department of Gastroenterology Instituto Nacional de Ciencias Médicas y Nutrición Salvador ZubiránMexico 14080Mexico

Coronavirus disease 2019(COVID-19),caused by the highly pathogenic severe acute respiratory syndrome coronavirus 2(SARS-CoV-2),primarily impacts the respiratory tract and can lead to severe outcomes such as acute respiratory distress syndrome,multiple organ failure,and *** extensive studies on the pathogenicity of SARS-CoV-2,its impact on the hepatobiliary system remains *** liver injury is commonly indicated by reduced albumin and elevated bilirubin and transaminase levels,the exact source of this damage is not fully *** mechanisms for injury include direct cytotoxicity,collateral damage from inflammation,drug-induced liver injury,and ischemia/***,evidence often relies on blood tests with liver enzyme *** this comprehensive review,we focused solely on the different histopathological manifestations of liver injury in COVID-19 patients,drawing from liver biopsies,complete autopsies,and in vitro liver *** present evidence of the direct impact of SARS-CoV-2 on the liver,substantiated by in vitro observations of viral entry mechanisms and the actual presence of viral particles in liver samples resulting in a variety of cellular changes,including mitochondrial swelling,endoplasmic reticulum dilatation,and hepatocyte *** ly,we describe the diverse liver pathology observed during COVID-19 infection,encompassing necrosis,steatosis,cholestasis,and lobular *** also discuss the emergence of long-term complications,notably COVID-19-related secondary sclerosing *** the histopathological liver changes occurring during COVID-19 infection is pivotal for improving patient recovery and guiding decision-making.

关键词： Liver SARS-CoV-2 COVID-19 Angiotensin-converting enzyme 2 Histopathology Liver biopsies Liver autopsy In vitro

The extractable hydrokinetic power from an oscillating membrane-based harvester

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Theoretical & Applied Mechanics Letters 2019年第2期9卷 66-70页

作者： Francisco J.arias salvador De Las Heras Department of Fluid Mechanics University of Catalonia

The extractable hydrokinetic power from an oscillating membrane in standing motion and induced by a water flow and its possible significance with regard energy harvesting is *** main attractiveness of such an energy harvester lies in the possibility of an inexpensive technology able to be used in those water flows which either because limitation of space(narrow channels) or a limited differential pressure drop with the surrounding but yet with a non negligible velocity are not well suited to be turbined. Utilizing a simplified geometrical model, an estimate of the extractable output density power per area of membrane was derived. Preliminary experiments were performed using a rectangular thin rubber membrane and for a typical domestic water intake as source. The experimental data quantitatively agree very well with the theoretical prediction where it was found that for water flows around 2 m/s the output power density from the membrane may be around 30 mW/cm^2 of membrane. Additional research and development is required in order to arrive at a reliable practical and commercial design.

关键词： Energy harvesting Residual waters Waste waters

A half century (1961-2011) of applying microsurgery to experimental liver research

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

World Journal of Hepatology 2012年第7期4卷 199-208页

作者： Maria-Angeles Aller Natalia arias Isabel Prieto salvador Agudo Carlos Gilsanz Laureano Lorente Jorge-Luis arias Jaime arias Surgery I Department School of Medicine Complutense University of Madrid 28040 Madrid Spain Neurosciences Lab Psychobiology Department School of Psychology University of Oviedo 33003 Oviedo Spain Surgery Department La Paz Hospital School of Medicine Autonomous University of Madrid 28049 Madrid Spain General Surgery Unit Sudeste Hospital Arganda del Rey 28500 Madrid Spain

The development of microsurgery has been dependent on experimental animals. Microsurgery could be a very valuable technique to improve experimental models of liver diseases. Microdissection and microsutures are the two main microsurgical techniques that can be considered for classifying the experimental models developed for liver research in the rat. Partial portal vein ligation, extrahepatic cholestasis and hepatectomies are all models based on microdissection. On the other hand, in portacaval shunts, orthotopic liver transplantation and partial heterotopic liver transplantation, the microsuture techniques stand out. By reducing surgical complications, these microsurgical techniques allow for improving the resulting experimental models. If good experimental models for liver research are successfully developed, the results obtained from their study might be particularly useful in patients with liver disease. Therefore experimental liver microsurgery could be an invaluable way to translate laboratory data on liver research into new clinical diagnostic and therapeutic strategies.

关键词： Microsurgery Portacaval shunts Cholestasis Hepatectomies Liver transplantation Portal hypertension

Preparation and Characterization of Flexible, Transparent and Thermally Stable Aromatic Co-polyamides

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Chinese Journal of Polymer Science 2019年第2期37卷 136-141页

作者： José Antonio Tec-Sánchez Andrés Iván Oliva arias Manuel Aguilar-Vega Juan Valerio Cauich-Rodríguez José Luis santiago-García Unidad de Materiales Centro de Investigación Científica de Yucatán A. C. Centro de Investigación y de Estudios Avanzados del IPN Unidad Mérida Departamento de Física Aplicada

Two aromatic co-polyamides were synthesized combining two diacid monomers containing bulky pendant groups, 5-(9,10-dihydro-9,10-ethanoanthracene-11,12-dicarboximido)isophthalic acid(DEAIA) and 5-tert-butylisophthalic acid(TERT), with 4,4′-(hexafluoroisopropylidene)dianiline(HFA) or 2,3,5,6-tetramethyl-1,4-phenylenediamine(Durene) by direct polycondensation. The structures of the obtained aromatic co-polyamides were confirmed by FTIR, Raman and 1 H-NMR. The co-copolyamide films, DHTH and DDTD, exhibited rms-roughness values between 0.94 and 1.60 nm, respectively. Moreover, they presented good thermal stability up to300 °C. Young's moduli of the co-polyamide films were between 4.1 and 4.3 GPa. X-ray diffraction results showed that the co-polyamide films were amorphous due to the incorporation of both bulky pendant groups, tert-butyl and dibenzobarrelene. The combination of bulky pendant groups provided intrinsically transparent co-polyamide films with a transmittance higher than 88% in the range of 400-780 *** to these outstanding film and optical properties, they are suggested to be flexible substrates in applications for solar cell and other portable electronic devices.

关键词： Co-polyamides Flexible films Transmittance Thermal stability Polycondensation

Population structuring of the ubiquitous stingless bee Tetragonisca angustula in southern Brazil as revealed by microsatellite and mitochondrial markers

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Insect Science 2017年第5期24卷 877-890页

作者： Flavio O. Francisco Leandro R. santiago Yuri M. Mizusawa Benjamin P. Oldroyd Maria C. arias Departamento de Genetica e Biologia Evolutiva Instituto de Biociencias Universidade de Sao Paulo Rua do MatZo 277 - sala 320Sao Paulo SP Brazi Behaviour and Genetics of Social Insects Lab School of Life and Environmental Sciences A 12 University of Sydney Sydney NSW Australia

Tetragonisca angustula is one of the most widespread stingless bees in the Neotropics. This species swarms frequently and is extremely successful in urban envi- ronments. In addition, it is one of the most popular stingless bee species for beekeeping in Latin America, so nest transportation and trading is common. Nest transportation can change the genetic structure of the host population, reducing inbreeding and increasing homogenization. Here, we evaluate the genetic structure of 17 geographic populations of T. angustula in southern Brazil to quantify the level of genetic differentiation between populations. Analyses were conducted on partially sequenced mitochondrial genes and 11 microsatellite loci of 1002 workers from 457 sites distributed on the mainland and on 3 islands. Our results show that T. angustula populations are highly differentiated as demon- strated by mitochondrial DNA （mtDNA） and microsatellite markers. Of 73 haplotypes, 67 were population-specific. MtDNA diversity was low in 9 populations but microsatellite diversity was moderate to high in all populations. Microsatellite data suggest 10 genetic clusters and low level of gene flow throughout the studied area. However, physical barri- ers, such as rivers and mountain ranges, or the presence or absence of forest appear to be unrelated to population clusters. Factors such as low dispersal, different ecological con- ditions, and isolation by distance are most likely shaping the population structure of this species. Thus far, nest transportation has not influenced the general population structure in the studied area. However, due to the genetic structure we found, we recommend that nest transportation should only occur within and between populations that are genetically similar.

关键词： genetic structure islands Meliponini microsatellites mtDNA nest transportation

Morphoagronomic characterization and wholegenome resequencing of eight highly diverse wild and weedy *** and *** *** accessions used for the first interspecific tomato MAGIC population

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Horticulture Research 2020年第1期7卷 477-492页

作者： Pietro Gramazio Leandro Pereira-Dias santiago Vilanova Jaime Prohens salvador Soler Javier Esteras Alfonso Garmendia María JoséDíez Faculty of Life and Environmental Sciences University of Tsukuba1-1-1 Tennodai305-8572 TsukubaJapan Instituto de Conservación y Mejora de la Agrodiversidad Valenciana Universitat Politècnica de ValènciaCamino de Vera 1446022 ValenciaSpain Departamento de Ecosistemas Agroforestales Universitat Politècnica de ValènciaCamino de Vera 1446022 ValenciaSpain Instituto Agroforestal Mediterráneo Universitat Politècnica de ValènciaCamino de Vera 1446022 ValenciaSpain

The wild Solanum pimpinellifolium(SP)and the weedy *** ***(SLC)are largely unexploited genetic reservoirs easily accessible to breeders,as they are fully cross-compatible with cultivated tomato(*** ***).We performed a comprehensive morphological and genomic characterization of four wild SP and four weedy SLC accessions,selected to maximize the range of variation of both *** eight accessions are the founders of the first tomato interspecific multi-parent advanced generation inter-cross(MAGIC)*** morphoagronomic characterization was carried out with 39 descriptors to assess plant,inflorescence,fruit and agronomic traits,revealing the broad range of diversity *** of the morphological variation observed in SP was likely associated to the adaptation of the accessions to different environments,while in the case of SLC to both human activity and adaptation to the ***-genome resequencing of the eight accessions revealed over 12 million variants,ranging from 1.2 to 1.9 million variants in SLC and from 3.1 to 4.8 million in SP,being 46.3%of them(4,897,803)private *** genetic principal component analysis also confirmed the high diversity of SP and the complex evolutionary history of *** was also reflected in the analysis of the potential footprint of common ancestors or old introgressions identified within and between the two *** functional characterization of the variants revealed a significative enrichment of GO terms related to changes in cell walls that would have been negatively selected during domestication and *** comprehensive morphoagronomic and genetic characterization of these accessions will be of great relevance for the genetic analysis of the first interspecific MAGIC population of tomato and provides valuable knowledge and tools to the tomato community for genetic and genomic studies and for breeding purposes.

关键词： agronomic breeding MAGIC

Duodenal biopsy may be avoided when high transglutaminase antibody titers are present

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

World Journal of Gastroenterology 2009年第38期15卷 4775-4780页

作者： santiago Vivas Jose G Ruiz de Morales Sabino Riestra Laura arias Dolores Fuentes Noemi Alvarez Sara Calleja Mercedes Hernando Blanca Herrero Javier Casqueiro Luis Rodrigo Department of Gastroenterology Hospital de León Institute of Biomedicine University of León Immunology Section Hospital de León Department of Gastroenterology Hospital Universitario Central de Asturias Department of Pathology Hospital de León Department of Pediatrics Hospital de León Faculty of Biology and Environmental Sciences University of León

AIM:To evaluate the predictive value of tissue transglutaminase (tTG) antibodies for villous atrophy in adult and pediatric populations to determine if duodenal biopsy can be ***: A total of 324 patients with celiac disease (CD; 97 children and 227 adults) were recruited prospectively at two tertiary centers. Human IgA class anti-tTG antibody measurement and upper gastrointestinal endoscopy were performed at diagnosis. A second biopsy was performed in 40 asymptomatic adults on a gluten-free diet (GFD) and with normal tTG ***: Adults showed less severe histopathology (26% vs 63%, P<0.0001) and lower tTG antibody titers than children. Levels of tTG antibody correlated with Marsh type in both populations (r=0.661, P<0.0001). Multiple logistic regression revealed that only tTG antibody was an independent predictor for Marsh type 3 lesions, but clinical presentation type and age were not. A cut-off point of 30 U tTG antibody yielded the highest area under the receiver operating characteristic curve (0.854). Based on the predictive value of this cut-off point, up to 95% of children and 53% of adults would be correctly diagnosed without biopsy. Despite GFDs and decreased tTG antibody levels, 25% of the adults did not recover from villous atrophy during the second year after ***: Strongly positive tTG antibody titers might be sufficient for CD diagnosis in children. However, duodenal biopsy cannot be avoided in adults because disease presentation and monitoring are different.

关键词： Biopsy Celiac disease Diagnosis Duodenum Transglutaminases

Release of interleukin-10 and neurotrophic factors in the choroid plexus：possible inductors of neurogenesis following copolymer-1 immunization after cerebral ischemia

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Neural Regeneration Research 2018年第10期13卷 1743-1752页

作者： Yolanda Cruz Edna E.García Jessica V.Gálvez Stella V.arias-santiago Horacio G.Carvajal Raúl Silva-García Herlinda Bonilla-Jaime Julio Rojas-Castaneda Antonio Ibarra Centro de Investigación en Ciencias de la Salud (CICSA) FCSUniversidad Anáhuac México Norte Huixquilucan Estado de México México Lab. De Biologia de la reproducción UAMI. Ciudad de México México Hospital de Pediatría CMN Siglo XXI. Ciudad de México México Subdirección de Medicina Experimental Instituto Nacional de PediatríaCiudad de México México Doctorado en Ciencias Biológicas División de Ciencias Biológicas y de la SaludUniversidad Autónoma MetropolitanaUnidad Iztapalapa.Ciudad de México México

Copolymer-1（Cop-1） is a peptide with immunomodulatory properties, approved by the Food and Drug Administration of United States in the treatment of multiple sclerosis. Cop-1 has been shown to exert neuroprotective effects and induce neurogenesis in cerebral ischemia models. Nevertheless, the mechanism involved in the neurogenic action of this compound remains unknown. The choroid plexus（CP） is a network of cells that constitute the interphase between the immune and central nervous systems, with the ability to mediate neurogenesis through the release of cytokines and growth factors. Therefore, the CP could play a role in Cop-1-induced neurogenesis. In order to determine the participation of the CP in the induction of neurogenesis after Cop-1 immunization, we evaluated the gene expression of various growth factors（brain-derived neurotrophic factor, insulin-like growth factor 1, neurotrophin-3） and cytokines（tumor necrosis factor alpha, interferon-gamma, interleukin-4（IL-4）, IL-10 and IL-17）, in the CP at 14 days after ischemia. Furthermore, we analyzed the correlation between the expression of these genes and neurogenesis. Our results showed that Cop-1 was capable of stimulating an upregulation in the expression of the genes encoding for brain-derived neurotrophic factor, insulin-like growth factor 1, neurotrophin-3 and IL-10 in the CP, which correlated with an increase in neurogenesis in the subventricular and subgranular zone. As well, we observed a downregulation of IL-17 gene expression. This study demonstrates the effect of Cop-1 on the expression of growth factors and IL-10 in the CP, in the same way, presents a possible mechanism involved in the neurogenic effect of Cop-1.

关键词： choroid plexus growth factors immunomodulation protective autoimmunity Cop-1 Copaxone stroke glatiramer acetate t MCAo focal cerebral ischemia