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Shifts in the Holstein dairy cow milk fat globule membrane proteome that occur during the first week of lactation are affected by parity

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Journal of Animal Science and Biotechnology 2021年第2期12卷 534-548页

作者： Mallory C.Honan Megan J.Fahey Amanda J.Fischer-Tlustos Michael A.Steele sabrina l.greenwood Department of Animal and Veterinary Sciences The University of VermontBurlingtonVT 05405USA Department of Animal Biosciences University of GuelphGuelphON N1G 2W1Canada Department of Agriculture Food&Nutritional ScienceUniversity of AlbertaEdmontonAB T6G 2P5Canada

Background:The milk fat globule membrane(MFGM)proteomes of colostrum and transition milk are rich sources of proteins that are likely important for neonatal calf *** addition,characterization of these proteomes could also yield valuable information regarding mammary gland physiology of the early postpartum lactating *** objectives of this research were to characterize the MFGM proteomes of colostrum and transition milk through sample collections at four timepoints postpartum,including the first milking(M1,colostrum),second milking(M2,transition milk),fourth milking(M4,transition milk),and fourteenth milking(M14,mature milk),and compare these proteomes between multiparous(MP;n=10)and primiparous(PP;n=10)Holstein dairy *** MFGM proteins were labeled using Tandem Mass tagging and analyzed using liquid chromatography-tandem mass spectrometry(lC-MS/MS).Protein identification was completed using MASCOT and Sequest in Proteome Discoverer *** scaled abundance values were analyzed using PROC MIXED in SAS to determine the effects of milking(MIl),parity(PAR),and MIl×*** adaptive false-discovery rate(FDR)-adjusted P values were determined using PROC *** characterization and bioinformatic analysis were completed using a combination of PANTHER,Blast,and ***:A total of 104 common proteins were identified in each of the MFGM *** analysis revealed that 70.2%of identified proteins were affected by *** these,78.1%were lower in M14 compared with M1,including immune-related proteins lactotransferrin,lactadherin and *** affected 44.2%of *** the proteins affected by PAR,84.8%were higher in MP cows compared with PP cows,including apolipoprotein E and histones 2A,2B,3,and 4 *** subfamily 1 member 1A and annexin 5 were higher in samples from PP ***×parity affected 32.7%of identified proteins,including lactotransferrin,gelsolin,vitamin D binding protein,and S100 ***

关键词： Colostrum lC-MS/MS Parity

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Adsorption of yttrium(Y^(3+)) and concentration of rare earth elements from phosphogypsum using chitin and chitin aerogel

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Journal of Rare Earths 2024年第4期42卷 775-782页

作者： Glaydson S.dos Reis Diana Pinto sabrina F.lütke Eder C.lima luis F.O.Silva Irineu A.S.De Brum Guilherme l.Dotto Department of Forest Biomaterials and Technology Biomass Technology Centre Swedish University of Agricultural SciencesUmeaE13Swedn Universidad De la Costa Calle 58#55-66080002BarranquillaAtlanticoColombia Research Group on Adsorptive and Catalytic Process Engineering(ENGEPAC) Federal University of Santa MariaAv.Roraima100079705900Santa MariaRSBrazil Institute of Chemistry Federal University of Rio Grande do Sul90040060Porto AlegreBrazil

Chitin powder and chitin-aerogel were prepared from shrimp wastes and used to uptake Y^(3+)from aqueous solutions and concentrate this rare earth element from phosphogypsum(PG).Chitin aerogel displays a specific surface area of 945 m^(2)/g,while chitin powder is 3.6 m^(2)/g,which largely influences its adsorption *** the adsorption in synthetic solutions,the effect of pH on Y^(3+)removal is strong for chitin powder *** contrast,no big pH influence was detected for chitin *** interactions and chelation can highlight the proposed mechanism of Y^(3+)on chitin adsorbents for the powder and ***,in addition to these interactions,pore filling/pore diffusion is the main mechanism of Y^(3+)removal in the chitin *** aerogel is efficient in concentrating 8 times the Y^(3+)from PG,a very complex *** complex chitin aerogel-Y^(3+)can be a secondary source of rare earth elements for other applications.

关键词： Rare earths Sustainable material Chitin powder Chitin porous aerogel Yttrium adsorption and recovery

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Conservative management of spinal pathology with autologous conditioned serum: A systematic review of the literature

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World Journal of Orthopedics 2024年第9期15卷 870-881页

作者： Christian J Rajkovic Matthew l Merckling Alyssa W lee Galadu Subah Aryan Malhotra Zachary D Thomas sabrina l Zeller John V Wainwright Merritt D Kinon Department of Neurosurgery Westchester Medical CenterValhallaNY 10595United States

BACKGROUND Chronic inflammatory pain is associated with increased expression of interleukin(Il)-1,an inflammatory cytokine,and activity on its receptor(Il-1R).In response,the body produces Il-1R antagonist(Il-1Ra)to reduce this *** conditioned serum(ACS)is the only biologic therapy for spinal pathologies that enhances the action of endogenous Il-1Ra reserves to improve *** systematic review investigates the effectiveness of ACS in treating pain and dis-ability caused by spinal *** To evaluate the use of ACS as a conservative management option for spinal *** A systematic review of PubMed/Medline was performed to identify studies inve-stigating administration of ACS for treatment of any spinal *** Six articles were included,comprising 684 patients treated with epidural(n=133)or transforaminal(n=551)ACS *** had an average age of 54.0 years with slight female predominance(53.2%).The lumbar spine was most com-monly treated,with 567 patients(82.9%)receiving injections for lumbar radicu-lopathy(n=67),degenerative disc disease(DDD)(n=372),or spinal stenosis(n=128);cervical injections were performed in 109 patients(15.9%).Mean(SD)follow-up was 21.7(4.8)weeks from first ACS *** studies investigating mecha-nical lumbar and lumbar or cervical radicular pain reported significant pain re-duction at final follow-up compared to *** achieved comparable or su-perior results to lumbar epidural steroid *** events were reported in 21 patients(3.1%),with no serious adverse *** ACS injection is a safe and effective intervention for pain reduction in many spinal pathologies,including cervical and lumbar radiculopathies.

关键词： Spine Autologous conditioned serum Orthokine Regenokine Epidural steroid injection Interleukin-1 Inter-leukin-1 receptor antagonist

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First light Curve Analysis of NSVS 8294044,V1023 Her,and V1397 Her Contact Binary Systems

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Research in Astronomy and Astrophysics 2024年第5期24卷 1-12页

作者： Atila Poro sabrina Baudart Mahshid Nourmohammad Zahra Sabaghpour Arani Fatemeh Farhadi Selda Ranjbar Salehian Ahmad Sarostad Saeideh Ranjbaryan Iri Olya Maryam Hadizadeh AmirHossein Khodaei Astronomy Department of the Raderon AI lab. BurnabyBCCanada Double Stars Committee SociétéAstronomique de FranceParisFrance Independent Astrophysics Researcher IsfahanIran Akhtaran Astronomical Society Isfahan ProvinceAran and BidgolIran Department of Physics Faculty of ScienceZanjan UniversityZanjanIran Astronomy Students'Scientific Association University of TabrizEast Azerbaijan ProvinceTabrizIran Yazd Desert Night Sky Astronomy Institute Yazd ProvinceYazdIran Ayaz Astronomical Association East Azerbaijan ProvinceTabrizIran Khayyam Astronomy Association FarsFasaIran

The first photometric light curve investigation of the NSVS 8294044,V1023 Her,and V1397 Her binary systems is *** used ground-based observations for the NSVS 8294044 system and Transiting Exoplanet Survey Satellite data for V1023 Her and V1397 *** primary and secondary times of minima were extracted from al the data,and,by collecting the literature,a new ephemeris was computed for each *** fits for the O-C diagrams were conducted using the Markov Chain Monte Carlo (MCMC) *** curve solutions were performed using the PHysics Of Eclipsing BinariEs Python code and the MCMC *** systems were found to be contact binary stars based on the fillout factor and mass ratio.V1023 Her showed the O’Connell effect and a cold starspot on the secondary component was required for the light curve *** absolute parameters of the system were estimated based on an empirical relationship between orbital period and *** presented a new T–M equation based on a sample of 428 contact binary systems and found that our three target systems were in good agreement with the *** positions of the systems were also depicted on the M–l,M–R,q–l_(ratio),and M_(tot)–J_(0)diagrams in the logarithmic scales.

关键词： (stars:)binaries eclipsing-techniques photometric-stars individual(NSVS 8294044)

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BSN:Photometric light Curve Analysis of Two Contact Binary Systems lS Del and V997 Cyg

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Research in Astronomy and Astrophysics 2024年第4期24卷 192-213页

作者： Atila Poro Mehmet Tanriver Elham Sarvari Shayan Zavvarei Hossein Azarara laurent Corp sabrina Baudart Asma Ababafi Nazanin Kahali Poor Fariba Zare Ahmet Bulut Ahmet Keskin Astronomy Department of the Raderon AI lab. BurnabyBC.Canada Department of Astronomy and Space Science Faculty of ScienceErciyes UniversityKayseri TR-38039Turkiye Erciyes University Astronomy and Space Science Observatory Application and Research CenterKayseri TR-38039Turkiye Independent Astrophysics Researcher TehranIran Department of Physics Shahid Beheshti UniversityTehranIran Faculty of Physics Shahid Bahonar University of KermanP.O.Box 76175KermanIran Double Stars Committee SociétéAstronomique de FranceParisFrance Dez Astronomical Association Khouzestan ProvinceDezfulIran Department of Physics Amirkabir University of TechnologyTehranIran Department of Physics Faculty of SciencesCanakkale Onsekiz Mart UniversityTerzioglu KampusuTR-17020CanakkaleTurkiye Astrophysics Research Center and Observatory Canakkale Onsekiz Mart UniversityTerzioğlu KampusuTR-17020CanakkaleTürkiye

The light curve analyses and orbital period variations for two contact binary stars,lS Del and V997 Cyg,are presented in this work which was conducted in the frame of the Binary Systems of South and North project Ground-based photometric observations were performed at two observatories in *** used the Transiting Exoplanet Survey Satellite(TESS)data for extracting times of minima and light curve analysis of the targe *** O-C diagram for both systems displays a parabolic *** Del and V997 Cyg’s orbital periods are increasing at rates of dP/dt=7.20×10^(-08)days yr^(-1)and dP/dt=2.54×10^(-08)days yr^(-1),respectively Therefore,it can be concluded that mass is being transferred from the less massive star to the more massive component with a rate of dM/dt=-1.96×10^(-7)M_(⊙)yr^(-1)for the lS Del system,and dM/dt=-3.83×10^(-7)M_(⊙)yr-1for V997 *** parameters of a third possible object in the system are also *** PHysics Of Eclipsing BinariEs Python code was used to analyze the light *** light curve solutions needed a cold starspot due to the asymmetry in the lS Del system’s light curve *** mass ratio fill-out factor,and star temperature all indicate that both systems are contact binary types in this *** methods were applied to estimate the absolute parameters of the systems:one method relied on the parallax of Gaia DR3,and the other used a P-M *** positions of the systems are also depicted on the M-l,M-R q-l_(ratio),and logM_(tot)-logJ_(0)*** recommend that further observations and investigations be done on the existence of a fourth body in this system.

关键词： (stars:)binaries:eclipsing methods:observational stars:individual(lS Del and V997 Cyg)

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light Perception:A Matter of Time

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Molecular Plant 2020年第3期13卷 363-385页

作者： sabrina E.Sanchez Matias l.Rugnone Steve A.Kay Department of Neurology Keck School of MedicineUniversity of Southern CaliforniaLos AngelesCAUSA

Optimizing the perception of external cues and regulating physiology accordingly help plants to cope with the constantly changing environmental conditions to which they are *** array of photoreceptors and intricate signaling pathways allow plants to convey the surrounding light information and synchronize an endogenous timekeeping system known as the circadian *** biological clock integrates multiple cues to modulate a myriad of downstream responses,timing them to occur at the best moment of the day and the ***,the mechanism underlying entrainment of the light-mediated clock is not *** review addresses known interactions between the light-signaling and circadian-clock networks,focusing on the role of light in clock entrainment and known molecular players in this process.

关键词： circadian clock light signaling Arabidopsis thaliana light entrainment photoreceptors signal integration

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Changes in skin levels of two neutotrophins (glial cell line derived neurotrohic factor and neurotrophin-3) cause alterations in cutaneous neuron responses to mechanical stimuli

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生理学报 2008年第5期60卷 584-596页

作者： Jeffrey lawson sabrina l. Mcllwrath H. Richard Koerber Department of Neurobiology University of Pittsburgh School of Medicine Pittsburgh PA 15261 USA

Neurotrophins are important for the development and maintenance of both high and low threshold mechanoreceptors(HTMRs and lTMRs).In this series of studies,the effects of constitutive overexpression of two different neurotrophins,neurotrophin-3(NT-3) and glial cell line derived neurotrohic factor(GDNF),were *** studies indicated that both of them may be implicated in the normal development of mouse dorsal root ganglion(DRG) *** from mice transgenically altered to overexpress NT-3 or GDNF(NT-3-OE or GDNF-OE mice) in the skin were examined using several physiological,immunohistochemi-cal and molecular *** vivo skin/nerve/DRG/spinal cord and skin/nerve preparations were used to determine the response characteristics of the cutaneous neurons;immunohistochemistry was used to examine the biochemical phenotype of DRG cells and the skin;RT-PCR was used to examine the levels of candidate ion channels in skin and DRG that may correlate with changes in physiological *** GDNF-OE mice,I-isolectin B4(IB4)-immunopositive C-HTMRs(nociceptors),a large percentage of which are sensitive to GDNF,had significantly lower mechanical thresholds than wildtype(WT) *** thresholds for the same cells were not *** sensitivity changes in GDNF-OE mice were correlated with significant increases in acid sensing ion channels 2a(ASIC2a) and 2b(ASIC2b) and transient receptor potential channel A1(TRPA1),all of which are putative mechanosensitive ion *** of NT-3 affected the responses of A-lTMRs and A-HTMRs,but had no effect on *** adapting type 1(SA1) lTMRs and A-HTMRs had increased mechanical sensitivity compared to *** sensitivity was correlated with significant increases in acid-sensing ion channels ASIC1 and *** data indicates that both neurotrophins play roles in determining mechanical thresholds of cutaneous HTMRs and lTMRs and that sensitivity changes involve the ASIC family of pu

关键词：皮肤胶质细胞神经元反应机械性刺激

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Extracellular matrix and biomimetic engineering microenvironment for neuronal differentiation

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Neural Regeneration Research 2020年第4期15卷 573-585页

作者： Deepak Jain sabrina Mattiassi Eyleen l.Goh Evelyn K.F.Yim Department of Chemical Engineering University of WaterlooWaterlooOntarioCanada Neuroscience Academic Clinical Programme Duke-NUS Medical SchoolSingapore

Extracellular matrix(ECM)influences cell differentiation through its structural and biochemical *** nervous system,neuronal behavior is influenced by these ECMs structures which are present in a meshwork,fibrous,or tubular forms encompassing specific molecular *** addition to contact guidance,ECM composition and structures also exert its effect on neuronal *** short report reviewed the native ECM structure and composition in central nervous system and peripheral nervous system,and their impact on neural regeneration and neuronal *** topographies,stem cells have been differentiated to ***,focussing on engineered biomimicking topographies,we highlighted the role of anisotropic topographies in stem cell differentiation to neurons and its recent temporal application for efficient neuronal differentiation.

关键词： biomimetic platforms biophysical cues contact guidance extracellular matrix neuronal development neural regeneration neural stem cell niche neuronal differentiation neuronal maturation stem cell topography

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Sediment generation and soil mound denudation in areas of high-density tree throw along a river valley in the Jura Mountains, Switzerland

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Journal of Mountain Science 2021年第2期18卷 377-391页

作者： Philip greenwood Jan BAUER Nikolaus J KUHN Physical Geography&Environmental Change Research Group Department of Environmental SciencesUniversity of BaselKlingelbergstrasse 27Basel-4056Switzerland

A preliminary field-based investigation was undertaken in a small(<10 km^(2))river valley located in the mountainous Jura region of northwest *** aims of the work were to assess sediment generation and annual sediment... 详细信息

A preliminary field-based investigation was undertaken in a small(lley located in the mountainous Jura region of northwest *** aims of the work were to assess sediment generation and annual sediment transport rates by tree throw on forested hillslopes,and to document surface hydrology characteristics on four fresh soil mounds associated with recent tree throws over a 24-day monitoring *** the soil mounds,average sediment recovery ranged from 7.7-28.2 g(dry weight),equivalent to a suspended sediment concentration of 145.2-327.8 g l^(-1),and runoff coefficients ranged from 1.0%-4.2%.Based on a soil bulk density value of 1,044 kg m^(-3),upslope runoff generation areas were denuded by an average 0.14 mm by the end of the 24-day monitoring period,representing an erosion rate equivalent to 2.1 mm yr^(-1).A ca.50 cm high soil mound could therefore feasibly persist for around 200-250 *** tree throw work,the dimensions of 215 individual tree throws were measured and their locations mapped in 12 separate locations along the river valley representing a cumulative area equivalent to 5.3 ha(***,43 per ha).Tree throws generated a total of 20.1 m^(3) of fine-sediment(lent of 3.8×10^(-4) m^(3) m^(-2).The process of tree throw was originally attributed to two extreme weather events that occurred in west and central Europe in late December *** the 18-year period since both storms,this represents an annual sediment transport rate of 2.7×10^(-5) m^(3) m^(-1) yr^(-1).Exploring the relationship with wind on fall direction,65.5%of tree throws(143)generally fell in a downslope direction irrespective of hillslope aspect on which they were *** infers that individual storms may not have been responsible for the majority of tree throws,but instead,could be associated with root *** the high density of tree throws and their relative maturity(average age 41 years),we hypothesise that once tr

关键词： Tree throw Hillslope processes Sediment generation mechanism Sediment transport rate Soil mound Ibach river valley

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A gene expression estimator of intramuscular fat percentage for use in both cattle and sheep

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Journal of Animal Science and Biotechnology 2014年第4期5卷 379-390页

作者： Bing Guo Kritaya Kongsuwan Paul l.greenwood Guanghong Zhou Wangang Zhang Brian P Dalrymple Key laboratory of Meat Processing and Quality Control Synergetic Innovation Center of Food Safety and NutritionCollege of Food Science and TechnologyNanjing Agriculture University CSIRO Animal Food and Health SciencesSt.Lucia QLD 4067Australia National Institute of Animal Health 50/2 KasetklangLadyaoBangkok 10900Thailand CSIRO Animal Food and Health SciencesArmidale NSW 2350Australia NSW Department of primary Industries Armidale NSW 2350Australia

Background：The expression of genes encoding proteins involved in triacyglyceride and fatty acid synthesis and storage in cattle muscle are correlated with intramuscular fat（IMF）%.Are the same genes also correlated with IMF%in sheep muscle,and can the same set of genes be used to estimate IMF%in both species？Results：The correlation between gene expression（microarray） and IMF%in the longissimus muscle（lM） of twenty sheep was *** integrated analysis of this dataset with an equivalent cattle correlation dataset and a cattle differential expression dataset was undertaken.A total of 30 genes were identified to be strongly correlated with IMF%in both cattle and *** overlap of genes was highly significant,8 of the 13 genes in the TAG gene set and 8 of the 13 genes in the FA gene set were in the top 100 and 500 genes respertively most correlated with IMF%in sheep,P-value = *** the 30 genes,CIDEA,THRSP,ACSM1,DGAT2 and FABP4 had the highest average rank in both *** the data from two small groups of Brahman cattle（control and Hormone growth promotant-treated[known to decrease IMF%in muscle]） and 22 animals in total,the utility of a direct measure and different estimators of IMF%（ultrasound and gene expression） to differentiate between the two groups were *** measured IMF%and IMF%estimated from ultrasound scanning could not discriminate between the two ***,using gene expression to estimate IMF%discriminated between the two *** the number of genes used to estimate IMF%from one to five significantly increased the discrimination power;but increasing the number of genes to 15 resulted in little further ***：We have demonstrated the utility of a comparative approach to identify robust estimators of IMF%in the lM in cattle and *** have also demonstrated a number of approaches（potentially applicable to much smaller groups of animals than conventional methods） to using gene expression to rank animal

关键词： Cattle Gene expression phenotype IMF% Sheep

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