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Signal Transduction and Targeted Therapy 2021年第8期6卷 2257-2258页

作者： sabrina ehnert Tina Histing Andreas K.Nussler Department of Trauma and Reconstructive Surgery Eberhard Karls University Tubingen Siegfried Weller Research InstituteBG Trauma Center TubingenTubingenGermany

In a study recently published in Nature,Shen and colleagues characterized a peri-arteriolar niche in the bone marrow with highly proliferative,short-lived,leptin receptor-and osteolectin(Oln)positive stromal cells,which improved bone regeneration and supported bacterial clearance after *** proving that maintenance of these cells critically required mechanical stimulation,which in turn was affected by age,1 the authors identified possible therapeutic targets for bone healing and regeneration.

关键词： stimulation healing clearance

Hepatotropic growth factors protect hepatocytes during inflammation by upregulation of antioxidative systems

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World Journal of Gastroenterology 2011年第17期17卷 2199-2205页

作者： Matthias Glanemann Daniel Knobeloch sabrina ehnert Mihaela Culmes Claudine Seeliger Daniel Seehofer Andreas K Nussler Department of General- Visceral-and Transplantation SurgeryCharitéCampus Virchow KlinikumUniversittsmedizin Berlin13353 BerlinGermany Department of Traumatology Klinikum rechts der IsarTechnische Universitt München81675 MunichGermany

AIM:To investigate effects of hepatotropic growth factors on radical production in rat hepatocytes during ***:Rat hepatocytes,isolated by collagenase perfusion,were incubated with a lipopolysaccharide(LPS)-containing cytokine mixture of interleukin-1β,tumor necrosis factor-α and interferon-γ to simulate sepsis and either co-incubated or pre-incubated with hepatotropic growth factors,*** growth factor,epidermal growth factor and/or transforming growth factor-α.Cells were analyzed for glutathione *** supernatants were assayed for produc-tion of reactive oxygen intermediates(ROIs) as well as NO2-,NO3-and *** determine cellular damage,release of aspartate aminotransferase(AST) into the culture medium was *** of nuclear factor(NF)-κB was measured by electrophoretic mobility shift ***:Rat hepatocytes treated with the LPS-containing cytokine mixture showed a significant increase in ROI and nitrogen oxide intermediate *** leakage was not significantly increased in cells treated with the LPS-containing cytokine mixture,independent of growth-factor ***,pretreatment with growth factors significantly reduced AST leakage and ROI formation while increasing cellular *** of growth factors did not result in increased NF-κB *** with growth factors further increased formation of NO2-,NO3-and S-nitrosothiols in hepatocytes stimulated with LPS-containing cytokine ***,we propose that,together with an increase in glutathione increased NO2-,NO3-formation might shift their metabolism towards non-toxic ***:Our data suggest that hepatotropic growth factors positively influence sepsis-induced hepatocellular injury by reducing cytotoxic ROI formation via induction of the cellular protective antioxidative systems.

关键词： Primary human hepatocytes Hepatocyte proliferation Cytokines Hepatotropic growth factors Nitric oxide Glutathione

Assessment of tobacco heating system 2.4 on osteogenic differentiation of mesenchymal stem cells and primary human osteoblasts compared to conventional cigarettes

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World Journal of Stem Cells 2020年第8期12卷 841-856页

作者： Romina H Aspera-Werz sabrina ehnert Monja Müller Sheng Zhu Tao Chen Weidong Weng Johann Jacoby Andreas K Nussler Department of Traumatology BG Trauma ClinicSiegfried Weller Institute for Trauma ResearchEberhard Karls Universität TübingenTübingen 72076Germany Department of Traumatology BG Trauma ClinicSiegfried Weller Institute for Trauma ResearchEberhard Karls Universität TübingenTübingen 71076Germany Institute for Clinical Epidemiology and Applied Biometry Eberhard Karls Universität TübingenTübingen 71076Germany

BACKGROUND Cigarette smoking(CS)is the most common method of consuming *** effects on bone integrity,increased incidence of fractures,and delayed fracture healing are all associated with *** 150 of the 6500 molecular species contained in cigarette smoke and identified as toxic compounds are inhaled by CS and,via the bloodstream,reach the skeletal *** technologies designed to develop a reduced-risk alternative for smokers are based on electronic nicotine delivery systems,such as e-cigarettes and tobacco heating systems(THS).THS are designed to heat tobacco instead of burning it,thereby reducing the levels of harmful toxic compounds *** To examine the effects of THS on osteoprogenitor cell viability and function compared to conventional *** Human immortalized mesenchymal stem cells(n=3)and primary human preosteoblasts isolated from cancellous bone samples from BG Unfall Klinik Tübingen(n=5)were osteogenically differentiated in vitro with aqueous extracts generated from either the THS 2.4“IQOS”or conventional“Marlboro”cigarettes for up to 21 *** viability was analyzed using resazurin conversion assay(mitochondrial activity)and calcein-AM staining(esterase activity).Osteogenic differentiation and bone cell function were evaluated using alkaline phosphatase(AP)activity,while matrix formation was analyzed through alizarin red *** cilia structure was examined by acetylatedα-tubulin immunofluorescent *** radical production was evaluated with 2’,7’-dichlorofluoresceindiacetate *** Our data clearly show that THS is significantly less toxic to bone cells than CS when analyzed by mitochondrial and esterase activity(P<0.001).No significant differences in cytotoxicity between the diverse flavors of THS were *** effects from THS on bone cell function were observed only at very high,non-physiological *** contrast,extracts from conventional cigarettes significantly reduced the A

关键词： Primary human osteoblast Cigarette smoke Tobacco heating system Mesenchymal stem cells Electronic nicotine delivery systems Bone

Effects of immune cells on mesenchymal stem cells during fracture healing

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World Journal of Stem Cells 2021年第11期13卷 1667-1695页

作者： sabrina ehnert Borna Relja Katharina Schmidt-Bleek Verena Fischer Anita Ignatius Caren Linnemann Helen Rinderknecht Markus Huber-Lang Miriam Kalbitz Tina Histing Andreas K Nussler Siegfried Weller Research Institute at the BG Trauma Center Tübingen Department of Trauma and Reconstructive SurgeryUniversity of TübingenTübingen 72076Germany Experimental Radiology Department of Radiology and Nuclear MedicineOttovon-Guericke UniversityMagdeburg 39120Germany Julius Wolff Institute and Berlin Institute of Health Center of Regenerative Therapies Charité-University Medicine BerlinBerlin 13353Germany Institute of Orthopedic Research and Biomechanics Ulm University Medical CenterUlm 89091Germany Institute for Clinical and Experimental Trauma-Immunology(ITI) University Hospital UlmUlm 89091Germany Department of Trauma and Orthopedic Surgery University Hospital Erlangen Friedrich-Alexander University Erlangen-Nürnberg(FAU)Erlangen 91054Germany

In vertebrates,bone is considered an osteoimmune system which encompasses functions of a locomotive organ,a mineral reservoir,a hormonal organ,a stem cell pool and a cradle for immune *** osteoimmune system is based on cooperatively acting bone and immune cells,cohabitating within the bone *** are highly interdependent,a fact that is confounded by shared progenitors,mediators,and signaling *** fracture healing requires the participation of all the precursors,immune and bone cells found in the osteoimmune *** evidence demonstrated that changes of the immune cell composition and function may negatively influence bone *** this review,first the interplay between different immune cell types and osteoprogenitor cells will be elaborated more *** separate paragraphs focus on the specific cell types,starting with the cells of the innate immune response followed by cells of the adaptive immune response,and the complement system as mediator between ***,a brief overview on the challenges of preclinical testing of immunebased therapeutic strategies to support fracture healing will be given.

关键词： Trauma Bones Immune response Mesenchymal stem cells Fracture healing

N-acetylcyteine and flavonoid rich diet:The protective effect of 15 different antioxidants on cigarette smoke-damaged primary human osteoblasts

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Advances in Bioscience and Biotechnology 2012年第8期3卷 1129-1139页

作者： sabrina ehnert Stefan Dobele Karl Friedrich Braun Britta Burkhardt Valeska Hofmann Mario Hausmann Jose Tomas Egana Ulrich Stockle Thomas Freude Andreas Klaus Nussler Department of Traumatology Eberhard Karls Universitat TübingenTübingenGermany Department of Traumatology MRITechnische Universitat MünchenMünchenGermany Department of Plastic Surgery and Hand Surgery Technische Universitat MünchenMünchenGermany FONDAP Center for Genome Regulation Facultad de CienciasUniversidad de ChileSantiagoChile

Cigarette consumption increases oxidative stress in many organs. Increased oxidative stress harms bone cells, which negatively affects bone-matter and -stability. This leads to an increased fracture risk and delayed fracture healing in smokers. A supporting therapy with antioxidants could be of great benefit for surgeons dealing with delayed fracture healing due to increased oxidative stress. In this article we complement and compare our published data with hitherto unpublished data and show the protective effect of 15 different antioxidants on cigarette smoke induced damage in primary human osteoblasts. Exposure to cigarette smoke medium (CSM) rapidly induces formation of ROS in osteoblasts in a concentration- and time-dependent manner. Massive cell damage is seen already after 4 h (EC50 ≈ 0.75 OD320). Pre-, co- and post-incubation with the different antioxidants reduces the formation of ROS and consequently improves the viability of the CSM exposed osteoblasts. Small compounds, e.g. N-acetylcysteine, proved highly effective if pre- or co-incubated before exposure to the CSM. Thus, they are good candidates for acute therapy support as they can be administered in high doses. However, our data suggest that a balanced daily diet could lead to an accumulation of various natural antioxidants (flavonoids) that effectively protect osteoblasts from oxidative stress-induced damage in all three settings investigated. Together with their partly phytoestrogenic properties this may even abate alterations in bone and thus reduce fracture risk on the long run.

关键词： Cigarette Smoke Osteoblasts Oxidative Stress N-Acetylcysteine Flavonoids

Two become one:combination of two risk factors in a new glaucoma animal model

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Neural Regeneration Research 2024年第5期19卷 982-983页

作者： Nils Kluge sabrina Reinehr Experimental Eye Research Institute University Eye HospitalRuhr-University BochumBochumGermany

Glaucoma is a group of eye diseases characterized by progressive loss of retinal ganglion cells(RGCs)and optic nerve *** this process,the visual field is reduced,and blindness may ultimately ***,glaucoma is the second leading cause of blindness,with about 80 million people *** is a multifactorial disease and due to its complexity,the exact pathomechanisms are not fully understood ***,different risk factors,such as elevated intraocular pressure(IOP),age,or myopia,have been identified to date(EGS,2021).

关键词： elevated exact glaucoma

Does HSP27 injection induce glaucoma damage in mice?

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Neural Regeneration Research 2024年第11期19卷 2347-2348页

作者： Stephanie C.Joachim sabrina Reinehr Experimental Eye Research Institute University Eye HospitalRuhr-University BochumBochumGermany

In a further aging society,excellent eyesight is an integral part of overall well-being and quality of *** good vision is crucial to maintaining mobility,independence,and mental *** can be several reasons for visual impairment in elderly people,these include age-related macular degeneration,the leading cause of vision loss among older adults,cataract,glaucoma,diabetic retinopathy,retinal detachment,and others.

关键词： HSP27 injection damage

Actin(g) toward a revised understanding of the role of cytoskeletal dynamics in neuronal bioenergetics

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Neural Regeneration Research 2024年第10期19卷 2109-2110页

作者： sabrina M.Holland Gianluca Gallo Department of Neural Sciences Shriners Pediatric Research CenterLewis Katz School of Medicine at Temple UniversityPhiladelphiaPAUSA

Neurons are energy-demanding *** in energy metabolism can quickly interrupt neuronal function,leading to cell death and *** instance,ischemia rapidly depletes adenosine triphosphate(ATP)thereby disrupting energy-dependent cellular processes crucial for homeostasis,and axon degeneration is preceded by a collapse of axonal ATP levels.

关键词： metabolism homeostasis degeneration

Silicon-photonics-enabled chip-based 3D printer

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Light(Science & Applications) 2024年第7期13卷 1320-1330页

作者： sabrina Corsetti Milica Notaros Tal Sneh Alex Stafford Zachariah A.Page Jelena Notaros Research Laboratory of Electronics Massachusetts Institute of TechnologyCambridgeMA 02139USA Department of Chemistry The University of Texas at AustinAustinTX 78712USA

Imagine if it were possible to create 3D objects in the palm of your hand within seconds using only a single photonic *** 3D printing has revolutionized the way we create in nearly every aspect of modern society,current 3D printers rely on large and complex mechanical systems to enable layer-by-layer addition of *** limits print speed,resolution,portability,form factor,and material *** there have been recent efforts in developing novel photocuring-based 3D printers that utilize light to transform matter from liquid resins to solid objects using advanced methods,they remain reliant on bulky and complex mechanical *** address these limitations,we combine the fields of silicon photonics and photochemistry to propose the first chip-based 3D *** proposed system consists of only a single millimeter-scale photonic chip without any moving parts that emits reconfigurable visible-light holograms up into a simple stationary resin well to enable non-mechanical 3D ***,we experimentally demonstrate a stereolithography-inspired proof-of-concept version of the chip-based 3D printer using a visible-light beam-steering integrated optical phased array and visible-light-curable resin,showing 3D printing using a chip-based system for the first *** work demonstrates the first steps towards a highlycompact,portable,and low-cost solution for the next generation of 3D printers.

关键词： printer printing visible

Compact and effective photon-resolved image scanning microscope

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Advanced Photonics 2024年第1期6卷 84-95页

作者： Giorgio Tortarolo Alessandro Zunino Simonluca Piazza Mattia Donato sabrina Zappone Agnieszka Pierzyńska-Mach Marco Castello Giuseppe Vicidomini Istituto Italiano di Tecnologia Molecular Microscopy and SpectroscopyGenoaItaly Genoa Instruments GenoaItaly University of Genoa Dipartimento di InformaticaBioingegneriaRobotica e Ingegneria dei SistemiGenoaItaly Istituto Italiano di Tecnologia Nanoscopy and NIC@IITGenoaItaly École Polytechnique Fédérale de Lausanne Laboratory of Experimental BiophysicsLausanneSwitzerland

Fluorescence confocal laser-scanning microscopy(LSM)is one of the most popular tools for life science *** popularity is expected to grow thanks to single-photon array detectors tailored for *** detectors offer unique single-photon spatiotemporal information,opening new perspectives for gentle and quantitative superresolution ***,a flawless recording of this information poses significant challenges for the microscope data acquisition(DAQ)*** present a DAQ module based on the digital frequency domain principle,able to record essential spatial and temporal features of *** use this module to extend the capabilities of established imaging techniques based on single-photon avalanche diode(SPAD)array detectors,such as fluorescence lifetime image scanning ***,we use the module to introduce a robust multispecies approach encoding the fluorophore excitation spectra in the time ***,we combine time-resolved stimulated emission depletion microscopy with image scanning microscopy,boosting spatial *** results demonstrate how a conventional fluorescence laser scanning microscope can transform into a simple,information-rich,superresolved imaging system with the simple addition of a SPAD array detector with a tailored data acquisition *** expected a blooming of advanced single-photon imaging techniques,which effectively harness all the sample information encoded in each photon.

关键词： fluorescence lifetime image scanning microscopy digital frequency domain single photon