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Electrospinning of Gelatin Functionalized with Silver Na-noparticles for Nanofiber Fabrication

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Modeling and Numerical Simulation of Material Science 2013年第4期3卷 95-105页

作者： Marwa A. Oraby ahmed I. Waley ahmed I. El-Dewany Ebtesam A. saad Bothaina M. Abd El-Hady Biomaterials Department National Research Center Giza Egypt Chemistry Department Faculty of Science Ain Shams University Cairo Egypt Natural Products Department National Research Center Giza Egypt Textile Department National Research Center Giza Egypt

The present paper deals with gelatin nanofibres functionalized with silver nanoparticles, prepared by electrospinning using solutions of gelatin mixed with silver nitrate (AgNO3). As a common solvent for gelatin and silver nitrate (AgNO3), a mixture of acetic acid and water (70:30 v/v) was selected. In this system, acetic acid was used as a solvent for gelatin, and at the same time reducing agent for silver ions in solution. Silver nanoparticles (nAg) were stabilized through a mechanism that involves an interaction of the oxygen atoms of the carbonyl groups of gelatin. The viscosity and the conductivity of the gelatinous solutions were found to increase with the solution concentration. There is an observed decrease in the viscosity of the nAg containing gelatin solutions with the aging time increasing, whereas the conductiity of the AgNO3—containing gelatin solutions was greater than that of the base gelatin solution. The gelatin nanofibres functionalized with silver nanoparticles were characterized by transmission electron microscopy (TEM), scanning electron microscopy (SEM), X-ray diffraction (XRD) and antimicrobial test. The results of investigations by TEM and XRD confirmed the presence of silver nanoparticles with diameters in the range of (2 - 10 nm), uniformly distributed over the surface of smooth nanofibres with an average diameter of 70 nm. The release of silver ions from both the 2- and 4-hrs crosslinked nAg containing gelatin fiber mats by a total immersion method in buffer and distilled water occurred rapidly during the first 60 minutes, and increased gradually afterwards. Lastly, the tests demonstrated that gelatin/Ag nanofibers have a good antimicrobial activity against some common bacteria found on burned wounds. The anti-bacterial activity of these materials was greatest against Staphylococcus aureus, followed by Escherichia coli, and Pseudomonas aeroginosa ≈ Candida albicans.

关键词： Electrospinning Gelatin Silver Nanofibers Nanoparticles Antimicrobial Activity

On Solutions of Generalized Bacterial Chemotaxis Model in a Semi-Solid Medium

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Applied Mathematics 2011年第12期2卷 1515-1521页

作者： ahmed M. A. El-Sayed saad Z. Rida Anas A. M. Arafa 不详

In this paper, the Adomian’s decomposition method has been developed to yield approximate solution of bacterial chemotaxis model of fractional order in a semi-solid medium. The fractional derivatives are described in the Caputo sense. The method introduces a promising tool for solving many linear and nonlinear fractional differential equations.

关键词： Decomposition Method Bacterial Chemotaxis Semi-Solid Medium Fractional Calculus

Spectrum of Upper GI Endoscopy in Pediatric Population at a Tertiary Care Centre in Pakistan

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Open Journal of Pediatrics 2014年第3期4卷 180-184页

作者： Muhammad Rehan Khan Shakeel ahmed Syed Rehan Ali Prem Kumar Maheshwari Muhammad saad Jamal Department of Pediatrics and Child Health The Aga Khan University Hospital Karachi Pakistan

Although upper GI endoscopy is considered to be a gold standard in diagnosis of many pediatric gastrointestinal disorders, there is limited data about its utility from developing countries. We carried out this retrospective study at Aga khan University Hospital, Karachi, Pakistan from 2008-2012. During the study period, a total of 200 procedures were performed. Mean age of patients was 8.5 years. 66% of patients received general anesthesia for the procedure. Failure to thrive with suspected coeliac disease was the most common indication for the procedure, seen in 31% of patients. Gastritis was the most common abnormal endoscopic finding, seen in 14.5% of patients. Gastritis was the most common histopathological finding on biopsy, seen in 31% of the patients (n = 62). Findings?were consistent with coeliac disease in 18% (n = 36), duodenitis, 10.5% (n = 21) and esophagitis, 4%?(n = 8) of patients. No immediate post procedure complication was noted in our study.

关键词： Upper GI Endoscopy Children Indications

The Impact of Pretransplant Disease Characteristics on the Outcome of Autologous Stem Cell Transplantation for Neuroblastoma with High-Risk Features: A Retrospective Model from a Limited Resources Country

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Journal of Cancer Therapy 2019年第6期10卷 422-432页

作者： ahmed Elhemaly Mahmoud Hammad Mohamed saad Zaghloul Maged Elshafie Naglaa Elkinaae Mohamed Khaled Alaa El-Haddad Department of Pediatric Oncology National Cancer InstituteCairo UniversityCairoEgypt Department of Pediatric Oncology Children’s Cancer Hospital EgyptCairoEgypt Department of Radiation Oncology National Cancer InstituteCairo UniversityCairoEgypt Department of Radiation Oncology Children’s Cancer Hospital EgyptCairo UniversityCairoEgypt Department of Surgical Oncology National Cancer InstituteCairo UniversityCairoEgypt Department of Surgical Oncology Children’s Cancer Hospital EgyptCairoEgypt Department of Pathology National Cancer InstituteCairo UniversityCairoEgypt Department of Pathology Children’s Cancer Hospital EgyptCairoEgypt Department of Research Children’s Cancer Hospital EgyptCairoEgypt

High-risk neuroblastoma still has poor survival outcome. Improvement of outcome is attributed to the consolidation of chemotherapy by autologous bone marrow transplant. Further improvement of the outcome by tandem autologous transplant is followed by immune therapy. We aimed with this study to correlate initial disease characteristics with the outcome of transplanted high-risk neuroblastoma. A retrospective analysis was done for 73 transplanted patients. Patients were treated in Children’s Cancer Hospital Egypt from July 2012 to July 2015. Seventy patients received Busulphan/Melphalan conditioning. The 3-year overall survival (OS) and event-free survival (EFS) was 63.3% and 51.3%, respectively. Disease stage did not impact the OS and EFS, P = 0.54 and 0.62 respectively. Status of MYCN did not reflect statistically on outcome for tumors with amplified compared to nonamplified (EFS, 49% and 63.1%, respectively). Response after induction chemotherapy pointed that patients who had objective response (complete response, very good partial response and partial response) were better compared to those with less response with EFS and OS of 53.3% and 64.2% compared to 49.3% and 63.5%, respectively, which may indicate that chemo-sensitive tumors have better outcome. By the end of the study, twenty-seven patients relapsed, out of them 25 patients died. Pretransplant risk features for neuroblastoma was nullified by autologous stem cell transplant. The modest outcome observed, highlights some limitations that need to be sorted out in countries with limited resources. The introduction of immune therapy and tandem transplant is needed to achieve a better outcome, yet it adds to more financial burden.

关键词： Neuroblastoma Limited-Resource Autologous Bone Marrow Transplant Busulphan Melphalan

Equivalence of String Classical and Quantum Energy beside Equivalence of Wave Packet and Relativistic Velocity in Eucleadian and Curved Space

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Natural Science 2020年第7期12卷 520-525页

作者： Mashair ahmed Mohammed Yousif Abeer Mohammed Khairy ahmed Zainab Mustapha Kurawa Omer A. M. Elnor Mubarak Dirar Abd-Alla Yagoub Ibrahim Mohammed Elfaki El-Tahir Mohammed Idriss ahmed Zoalnoon ahmed Abeid Allah saad Department of Physics Faculty of Science and Education at Alkurma Taif University Alkhurma Kingdom of Saudi Arabia Department of Physics Faculty of Art and Science at Al Muznab Al Qassim University Al Muznab Kingdom of Saudi Arabia Department of Physics School of Science Education Saadatu Rimi College of Education Kumbotso Nigeria Department of Physics Faculty of Science University of Bakhtalruda Ed Dueim Sudan Department of Physics Faculty of Science Sudan University of Science and Technology Khartoum Sudan Department of Natural and Applied Sciences College of Science and Humanities at Afif Sharga University Afif Kingdom of Saudi Arabia College of Arts and Sciences King Khalid University Dhahran Saudi Arabia

Plank quantum and classical string energy relations seem to be uncorrelated. This work correlated them. The relativistic energy-momentum relation has been used together with plank and de Brogglie hypothesis to prove that the wave group velocity is equal to the particle velocity in both ordinary and curved space. The plank energy relation is shown also to be related to the classical energy relation of an oscillating string. Starting from plank energy relation for n photons and performing integration, the expression of classical string energy was obtained. This means that one can treat electromagnetic waves as a collection of continuous photons having frequencies ranging from zero to w. Conversely, starting from classical string energy relation by differentiating it with respect to angular frequency, the plank quantum energy for n photons has been found. This means that the quanta results from separation of electromagnetic waves to single isolated waves. Each wave consists of n photons or quanta.

关键词： Plank Energy Classical Energy String Quanta Photons Electromagnetic Waves Curved Space Group Velocity Wave Packet

A Preliminary Documentation of the Coral Reefs from Libya

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Open Journal of Geology 2016年第4期6卷 260-269页

作者： Belkasim Khameiss William Hoyt saad K. El Ebaidi ahmed M. Muftah James Klaus Ann Budd Department of Geological Sciences Ball State University Muncie IN USA Earth and Atmospheric Sciences University of Northern Colorado Greeley CO USA Department of Earth Sciences Faculty of Science University of Benghazi Benghazi Libya Department of Geological Science University of Miami Coral Gables FL USA Department of Geoscience University of Iowa Iowa City IA USA

Corals studies in Libya are very limited, although they play an important role in the oil exploration as they form excellent reservoirs of coral reef buildups at some oil fields of Sirt Basin (e.g. Intisar “Idris” and Sahabi Fields). Both fields are produced from Paleocene coral reefs. Meanwhile, in Cyrenaica, corals are of less importance as they are not reported in subsurface tertiary rocks, which probably in the environmental settings of these sediments out of the core of reef as occurred in the surface. Meanwhile, corals are reported from older (Jurassic) subsurface successions as in Concession NC-152, but the cementation diagenesis leads to blocking and destroying the porosity. This study presents the first surface documentation work of eight scleractinian coral species from the exposed sediments in northern Libya, where sixtaxa is reported from Al Jabal al Akhdar region, these include a) an association of huge colonies of Caulastrea sp. and Stylophora sp., from the Middle Eocene Darnah Formation at West Darnah road cut section. Theco-existence of the fastCaulastrea sp. with the slow Stylophora sp. is due to the competition strategy;b) Antiguastrea sp. which is reported from the Oligocene Algal Limestone of Al Bayda Formation at Daryanah-Abyar Roadcut section;c) Cyphastrea sp. andAleveopora sp. from Oligo-Miocene Al Faidiyah Formation at Al Fatayah cement quarry and d) Tarbellastraea sp. From Middle Miocene Benghazi Formation at Benghazi Cement Quarry. In addition, two species Astraeaopora sp. and Actinacis paroraiare reported from the Upper Miocene sediments of formation “M” in As Sahabi area.

关键词： Cyrenaica Corals Benghazi Cement Quarry Persististrombus coronatus

Fibrinogen Levels in Hypertensive and Normotensive: A Cross-Sectional Study from El-Obied City, Sudan

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Journal of Biosciences and Medicines 2016年第2期4卷 28-32页

作者： ahmed Abdalla Agab Eldour Tarig Osman Khalafallah Hassan Mohammed Noja Eltayib Siddig Mohammed saad Mohieldin Elsayid Asaad Mohammed ahmed Abd Allah Babker Faculty of Medicine and Health Science Medical Laboratory Science Program University of Kordofan El-Obied Sudan Future Lab Medical Laboratories Co. Ltd. Al Kharj KSA Departments of Clinical Laboratory Sciences College of Applied Medical Sciences King Saud Bin Abdulaziz University for Health Sciences Riyadh KSA Department of Medical Laboratory Science Al-Ghad International College for Health Sciences Al-Madinah Al-Monawarah KSA

Hypertension (HTN) has been defined as a blood pressure level at which an otherwise healthy person would have an increased risk of cardiovascular disease that could be mitigated through blood pressure-lowering treatment. Worldwide, hypertension is one of the most common causes of death. The prevalence of hypertension and cardiovascular disease increases with age, and has been found to be higher in those of South Asian and African ancestry, and in Aboriginal populations. Hypertension is not only one of the most important risk factors for cardiovascular disease, but also the number one modifiable risk factor for stroke. Alterations in blood coagulation system have been reported in patients of hypertension. Fibrinogen has been identified as a major independent risk factor for cardiovascular diseases. This was a descriptive cross-sectional study, conducted in Elobied teaching hospital during period from March to June 2014. The aim of this study is to determine the level of fibrinogen among hypertensive patients. Seventy percent of the patients (about 35 patients) had high fibrinogen level above 400 mg/dl compared with the control and about 30% (15 patients) were within the normal range of 200 mg - 400 mg/dl. This study has concluded that there was a significant increase in fibrinogen level in hypertensive patients compared to control, while the gender has no effect on the level of fibrinogen.

关键词： Hypertensive Normotensive Fibrinogen Level El-Obied City Sudan

Effect of honey on oxidation, chlorination and nitration by purified equine myeloperoxidase

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Journal of Coastal Life Medicine 2017年第9期5卷 398-402页

作者： saad Aissat Hama Benbarek Thierry Franck Stephan Kohnen Didier Serteyn Moussa ahmed Ange Mouithys-Mickalad Institute of Veterinary Sciences Ibn Khaldoun UniversityTiaret14000Algeria Pharmacognosy and Api-Phytotherapy Research Laboratory Mostaganem UniversityMostaganemAlgeria Faculty of Sciences of Nature and Life Mascara UniversityMascara29000Algeria Centre for Oxygen Research and Development(CORD)Institute of Chemistry B6aUniversity of LiègeSart Tilman4000Liège1Belgium Department of Clinical Sciences Large Animal SurgeryFaculty of Veterinary MedicineB41University of LiègeSart Tilman4000Liège1Belgium Celabor Centre de Services Scientifique et Technique aux EntreprisesZoning Industriel de Petit-RechainAvenue du Parc384650HerveBelgium

Objective: To evaluate the antioxidant effect of honey using two classical methods generally used, and for the first time to test the effect of honey on the oxidation, chlorination and nitration by purified equine myeloperoxidase (MPO). Methods: The antioxidant activity of three Algerian honey samples (nectar honey, mixed honey and honeydew honey) was evaluated by two classical methods, the ferric- reducing/antioxidant power (FRAP) assay and 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical-scavenging capacity. Results: Honeydew honey had the highest reducing power and DPPH radical-scavenging activity, whereas nectar honey showed the lowest reducing power and DPPH radical-scavenging activity. All honey samples showed a significant inhibitory effect on the chlorination activity of equine MPO, but honeydew honey was the weakest inhibitor. The three samples were poorly inhibitor on the MPO oxidation and nitration activities, except for nectar honey that exerted an inhibitory effect at the highest tested concentration of 10%. These later results seem to contradict those obtained with DPPH and FRAP. Conclusions: The antioxidant capacity of honey is mainly due to the phenolic compounds and flavonoids it contained. It has been suggested that MPO might be involved in the antioxidant, not pro-oxidant, activity of phenolic compounds.

关键词： Honey Myeloperoxidase Chlorination Oxidation Nitration

Differential expression of cell cycle regulators in HCV-infection and related hepatocellular carcinoma

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World Journal of Hepatology 2010年第1期2卷 32-41页

作者： Azza E El Bassiouny Mona M Nosseir Mona K Zoheiry Noha A Ameen ahmed M Abdel-Hadi Ibrahim M Ibrahim Suher Zada Abdel-Hakeem saad El-Deen Nora E El-Bassiouni Department of Immunology Theodor Bilharz Research Institute Department of Pathology Central Laboratory Theodor Bilharz Research Institute Department of Hepato-Gastroenterology Theodor Bilharz Research Institute Biology Department American University Zoology Department Faculty of ScienceCairo University Department of Hematology Theodor Bilharz Research Institute

AIM:To investigate cell cycle proteins in chronic hepatitis C virus infection in order to analyze their role in the process of hepatocyte transformation and to characterize their prognostic properties. METHODS:Subjects of the current study included 50 cases of chronic hepatitis C(CHC) without cirrhosis,30 cases of CHC with liver cirrhosis(LC) ,and 30 cases of hepatitis C-related hepatocellular carcinoma(HCC) admitted to the Department of Hepato-Gastroenterology,Theodor Bilharz Research Institute(TBRI) ,Giza,*** wedge liver biopsies,taken during laparoscopic cholecystectomy,were also included as normal *** investigations including urine and stool analysis,liver function tests and prothrombin concentration;serologic markers for viral hepatitis and ultrasonography were done for all cases of the study together with immunohistochemical analysis using primary antibodies against Cyclin D1,Cyclin E,p21,p27 and Rb/p105 proteins. RESULTS:Normal wedge liver biopsies didn't express Cyclin E or Rb/p105 immunostaining but show positive staining for Cyclin D1,p21 and *** D1 expressed nuclear staining that was sequentially increased from CHC to LC(P<0.01) to HCC(P<0.001) cases;meanwhile,Cyclin E revealed nuclear positivity only in the case of HCCs patients that was directly correlated to Rb/p105 *** expression of p21 and p27 was significantly increased in CHC and LC cases compared to normal controls and HCCs with no significant difference between well-and poorlydifferentiated tumors.p21 showed only a nuclear pattern of staining,while,p27 presented with either cytoplasmic and/or nuclear reactivity in all studied *** analysis revealed a direct relation between Cyclin D1 and p21 in CHC cases(P<0.001) ,between Cyclin D1 and Cyclin E in HCCs(P<0.01);however,an inverserelationship was detected between Cyclin D1 and p21 or p27(P<0.001) and between p21 and Rb/p105(P<0.05) in HCCs. CONCLUSION:Upregulation of Cyclin D1 in CHC p

关键词： Chronic hepatitis C Liver cirrhosis Hepatocellular carcinoma Cell cycle Cyclin D1 Cyclin E p21 p27 Rb/p105