Differential expression of cell cycle regulators in HCV-infection and related hepatocellular carcinoma

作     者：Azza E El Bassiouny Mona M Nosseir Mona K Zoheiry Noha A Ameen Ahmed M Abdel-Hadi Ibrahim M Ibrahim Suher Zada Abdel-Hakeem Saad El-Deen Nora E El-Bassiouni 

作者机构：Department of ImmunologyTheodor Bilharz Research Institute Department of Pathology Central LaboratoryTheodor Bilharz Research Institute Department of Hepato-GastroenterologyTheodor Bilharz Research Institute Biology DepartmentAmerican University Zoology DepartmentFaculty of ScienceCairo University Department of HematologyTheodor Bilharz Research Institute 

出 版 物：《World Journal of Hepatology》 (世界肝病学杂志（英文版）（电子版）)

年 卷 期：2010年第2卷第1期

页      面：32-41页

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

基　　金：Supported by Theodor Bilharz Research Institute(Grant# 74D) in collaboration with the American University of Cairo 

主　　题：Chronic hepatitis C Liver cirrhosis Hepatocellular carcinoma Cell cycle Cyclin D1 Cyclin E p21 p27 Rb/p105 

摘      要：AIM:To investigate cell cycle proteins in chronic hepatitis C virus infection in order to analyze their role in the process of hepatocyte transformation and to characterize their prognostic properties. METHODS:Subjects of the current study included 50 cases of chronic hepatitis C(CHC) without cirrhosis,30 cases of CHC with liver cirrhosis(LC) ,and 30 cases of hepatitis C-related hepatocellular carcinoma(HCC) admitted to the Department of Hepato-Gastroenterology,Theodor Bilharz Research Institute(TBRI) ,Giza,*** wedge liver biopsies,taken during laparoscopic cholecystectomy,were also included as normal *** investigations including urine and stool analysis,liver function tests and prothrombin concentration;serologic markers for viral hepatitis and ultrasonography were done for all cases of the study together with immunohistochemical analysis using primary antibodies against Cyclin D1,Cyclin E,p21,p27 and Rb/p105 proteins. RESULTS:Normal wedge liver biopsies didn t express Cyclin E or Rb/p105 immunostaining but show positive staining for Cyclin D1,p21 and *** D1 expressed nuclear staining that was sequentially increased from CHC to LC(P0.01) to HCC(P0.001) cases;meanwhile,Cyclin E revealed nuclear positivity only in the case of HCCs patients that was directly correlated to Rb/p105 *** expression of p21 and p27 was significantly increased in CHC and LC cases compared to normal controls and HCCs with no significant difference between well-and poorlydifferentiated tumors.p21 showed only a nuclear pattern of staining,while,p27 presented with either cytoplasmic and/or nuclear reactivity in all studied *** analysis revealed a direct relation between Cyclin D1 and p21 in CHC cases(P0.001) ,between Cyclin D1 and Cyclin E in HCCs(P0.01);however,an inverserelationship was detected between Cyclin D1 and p21 or p27(P0.001) and between p21 and Rb/p105(P0.05) in HCCs. CONCLUSION:Upregulation of Cyclin D1 in CHC p