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Journal of Thermal Science 2024年第2期33卷 501-508页

作者： LI Weiwei MIAO Qi ZHANG Yelong DING Hongliang YANG sa LAI Yuan FENG Daili DING Yulong JIN Yi TAN Linghua School of Chemistry and Chemical Engineering Nanjing University of Science&TechnologyNanjing210094China national Special Superfine Powder Engineering Research Center Nanjing University of Science and TechnologyNanjing210094China nanjing Jinhe Energy Material Co. LtdNanjing210047China School of Chemical Engineering University of BirminghamBirminghamB152TTUK School of Energy&Environmental Engineering University of Science and Technology BeijingBeijing100083China

In this paper,a novel ternary eutectic salt na_(2)CO_(3)-Li_(2)CO_(3)-LiF was designed and investigated for concentrated solar power(CSP).The Factsage software was used to predict the composition and eutectic point of na_(2)CO_(3)-Li_(2)CO_(3)-*** microstructure,thermophysical properties,and thermal stability of eutectic salts were experimentally measured using various analytical *** a mass ratio of 57%:32%:11%,the eutectic salt exhibited excellent thermal storage properties with a fusion enthalpy of 413 J/g and a melting point of 426.8℃.The excellent thermal stability of the eutectic salt was reflected by a weight loss of only 0.8% at 600℃.

关键词： phase change materials ternary eutectic salt latent heat thermal stability

The jasmonate pathway promotes nodule symbiosis and suppresses host plant defense in Medicago truncatula

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Molecular Plant 2024年第8期17卷 1183-1203页

作者： Da Guo Jingrui Li Peng Liu Yuzhan Wang na Cao Xiangling Fang Tao Wang Jiangli Dong College of Biological Sciences China Agricultural UniversityBeijing 100193China State Key Laboratory of Plant Environmental Resilience College of Biological SciencesChina Agricultural UniversityBejing 100193China State Key Laboratory of Herbage Improvement and Grassland Agro-ecosystems College of Pastoral Agriculture Science and TechnologyLanzhou UniversityLanzhou730020China

Root nodule symbiosis(RNs)between legumes and rhizobia is a major source of nitrogen in agricultural *** symbiosis requires precise regulation of plant defense *** role of the defense hormone jasmonic acid(ja)in the immune response has been extensively *** research shows that ja can play either a positive or negative regulatory role in RNS depending on its concentration,but the molecular mechanisms remain to be *** this study,we found that inoculation with the rhizobia Sm1021 induces the ja pathway in Medicago truncatula,and blocking the ja pathway significantly reduces the number of infection *** in the MtMYc2 gene,which encodes a ja signaling master transcription factor,significantly inhibited rhizobia infection,terminal differentiation,and symbiotic cell *** Rna sequencing and chromatin immunoprecipitation sequencing,we discovered that MtMYc2 regulates the expression of nodule-specific MtDNF2,MtnaD1,and MtSymCRK to suppress host defense,while it activates MtDNF1 expression to regulate the maturation of MtNCRs,which in turn promotes bacteroid *** importantly,MtMYC2 participates in symbiotic signal transduction by promoting the expression of ***,the MtMYC2-MtiPD3 transcriptional regulatory module is specifically present in legumes,and the Mtmyc2 mutants are susceptible to the infection by the pathogen Rhizoctonia ***,these findings reveal the molecular mechanisms of how the ja pathway regulates RNS,broadening our understanding of the roles of ja in plant-microbe interactions.

关键词： jasmonic acid MYC2 symbiosis defense Medicago truncatula

MdbHLH162 connects the gibberellin and jasmonic acid signals to regulate anthocyanin biosynthesis in apple

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Journal of Integrative Plant Biology 2024年第2期66卷 265-284页

作者： Jian-Ping An Rui-Rui Xu Xiao-na Wang Xiao-Wei Zhang Chun-Xiang You Yuepeng Han Apple technology innovation center of Shandong Province College of Horticulture Science and EngineeringShandong Agricultural UniversityTai-An 271018China CAS Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture Wuhan Botanical GardenHubei Hongshan LaboratoryThe Innovative Academy of Seed Design of Chinese Academy of SciencesWuhan 430074China College of Biology and Oceanography Weifang UniversityWeifang 261061China

Anthocyanins are secondary metabolites induced by environmental stimuli and developmental *** positive regulators of anthocyanin biosynthesis have been reported,whereas the anthocyanin repressors have been *** the signal transduction pathways of gibberellin(GA)and jasmonic acid(ja)and their regulation of anthocyanin biosynthesis have been investigated,the cross-talk between GA and ja and the antagonistic mechanism of regulating anthocyanin biosynthesis remain to be *** this study,we identified the anthocyanin repressor MdbHLH162 in apple and revealed its molecular mechanism of regulating anthocyanin biosynthesis by integrating the GA and ja ***162 exerted passive repression by interacting with MdbHLH3 and MdbHLH33,which are two recognized positive regulators of anthocyanin ***162 negatively regulated anthocyanin biosynthesis by disrupting the formation of the anthocyanin-activated MdMYB1-MdbHLH3/33complexes and weakening transcriptional activation of the anthocyanin biosynthetic genes MdDFR and MdUF3GT by MdbHLH3 and *** GA repressor MdRGL2a antagonized MdbHLH162-mediated inhibition of anthocyanins by sequestering MdbHLH162 from the MdbHLH162-MdbHLH3/33 *** ja repressors MdjaZ1 and MdjaZ2 interfered with the antagonistic regulation of MdbHLH162 by MdRGL2a by titrating the formation of the MdRGL2a-MdbHLH162 *** findings reveal that MdbHLH162 integrates the GA and ja signals to negatively regulate anthocyanin *** study provides new information for discovering more anthocyanin biosynthesis repressors and explores the cross-talk between hormone signals.

关键词： anthocyanin biosynthesis bHLH transcription factor gibberellin jasmonic acid regulatory network transcriptional regulation

Suppression of Cs FAD3 in a ja-dependent manner, but not through the sa pathway, impairs drought stress tolerance in tea

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Journal of Integrative Agriculture 2024年第11期23卷 3737-3750页

作者： na Chang Xiaotian Pi Ziwen Zhou Yeyun Li Xianchen Zhang State Key Laboratory of Tea Plant Biology and Utilization Anhui Agricultural University

The growth and yield of tea plants are seriously limited by drought stress. Fatty acid desaturases（FADs） contribute to the mediation of membrane fluidity in response to different stresses, although the role of ω-3 FAD（Omega-3fatty acid desaturase）-mediated damage induced by drought stress in tea plants is poorly understood. In this study,drought stress significantly promoted the synthesis of C18:3（linolenic acid） and the expression level of *** experiments further demonstrated that CsFAD3 can convert C18:2 to C18:3, and that the 35S:GFP-CsFAD3fusion protein was localized in the endoplasmic reticulum of Nicotiana benthamiana cells. CsFAD3-silenced tea leaves exhibited poor drought tolerance, with a lower Fv/Fmand a higher malondialdehyde（MDA） content than the control plants. However, transgenic 35S:CsFAD3 Arabidopsis plants showed the opposite phenotypes. In addition,the jasmonic acid（ja） content and the expression levels of CsLOX2, CsLOX4, CsaOS, CsaOC3 and CsOPR2 were significantly reduced in CsFAD3-silenced leaves under drought stress. However, no substantial difference in the salicylic acid（sa） content was detected under normal or drought conditions. An analysis of Atcoi1（ja receptor） or Atnpr1（sa receptor） mutant Arabidopsis plants in 35S:CsFAD3 backgrounds further revealed that knockout of Atcoi1impaired the drought-tolerant phenotypes of CsFAD3 overexpression lines. Therefore, this study demonstrated that CsFAD3 plays a crucial role in drought tolerance by mediating ja pathways.

关键词： tea plants CsFAD3 drought stress jasmonic acid

刺玫根多糖对环磷酰胺诱导的免疫抑制小鼠的免疫调节作用

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天然产物研究与开发 2024年第2期36卷 196-205,292页

作者：查苏娜苏日娜齐和日玛彭斯格热希萨仁高娃内蒙古医科大学呼和浩特010110

采用水提醇沉法提取刺玫根粗多糖,以Sevage法和TCA法脱蛋白,进一步分级醇沉法分离纯化得到较纯的60%醇沉物(RDRP)进行结构鉴定和活性评价。苯酚浓硫酸法测定出刺玫根多糖RDRP总糖含量为94.1%;高效凝胶渗透色谱法测定表明主要含有重均分... 详细信息

采用水提醇沉法提取刺玫根粗多糖,以Sevage法和TCA法脱蛋白,进一步分级醇沉法分离纯化得到较纯的60%醇沉物(RDRP)进行结构鉴定和活性评价。苯酚浓硫酸法测定出刺玫根多糖RDRP总糖含量为94.1%;高效凝胶渗透色谱法测定表明主要含有重均分子量为13.1 kDa,相对分散度为1.2的多糖;通过高效离子色谱法和红外光谱测定,鉴定刺玫根多糖为由葡萄糖、阿拉伯糖、半乳糖、甘露糖、木糖等5种单糖组成的含有α-构型的中性吡喃多糖。环磷酰胺诱导的免疫抑制小鼠模型实验结果表明,与模型组相比,刺玫根多糖各剂量组小鼠的脾脏和胸腺指数明显升高(Psa法检测血清中免疫相关蛋白IgG、IgM、IgA以及细胞因子TNF-α、IFN-γ、IL-6与IL-2的分泌水平,结果显示,与模型组比较,刺玫根多糖组显著上调小鼠血清中免疫球蛋白IgA、IgG、IgM浓度水平(P<0.01)及IL-2、IFN-γ、TNF-α、IL-6的含量(P<0.01);Western blot法测定各组TLR4、MyD88、TRAF-6和NF-κB p65蛋白的表达水平,发现刺玫根多糖各剂量组脾脏中TLR4、TRAF-6蛋白极显著上升(P<0.01),高剂量组的MyD88蛋白极显著上升(P<0.01),其他组呈现上升趋势;刺玫根多糖各剂量组NF-κB p65蛋白均有上升趋势,因此其免疫调节作用机制可能与TLR/NF-κB通道有关。本研究初步阐明刺玫根多糖的基本结构特征和其免疫调节作用及其机制,为刺玫根多糖的开发应用提供一定价值的实验依据。

关键词：刺玫根多糖结构鉴定免疫调节 TLR/NF-κB

支气管肺泡灌洗联合俯卧位治疗儿童肺炎支原体肺炎伴肺不张疗效的前瞻性随机对照研究

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中国当代儿科杂志 2024年第1期26卷 31-36页

作者：萨日娜王爱琼高有汉李晓安呼格吉乐图鄂尔多斯市中心医院普儿科内蒙古鄂尔多斯017000 内蒙古医科大学研究生学院内蒙古呼和浩特010059 鄂尔多斯市中心医院康复科内蒙古鄂尔多斯017000

目的探讨支气管肺泡灌洗(bronchoalveolar lavage,BAL)联合俯卧位在儿童肺炎支原体肺炎(Mycoplasma pneumoniae pneumonia,MPP)伴肺不张中的疗效及对肺功能的影响。方法前瞻性选取2020年11月—2023年5月在内蒙古鄂尔多斯市中心医院住院... 详细信息

目的探讨支气管肺泡灌洗(bronchoalveolar lavage,BAL)联合俯卧位在儿童肺炎支原体肺炎(Mycoplasma pneumoniae pneumonia,MPP)伴肺不张中的疗效及对肺功能的影响。方法前瞻性选取2020年11月—2023年5月在内蒙古鄂尔多斯市中心医院住院治疗的94例MPP伴肺不张的患儿为研究对象,随机分为治疗组和对照组,每组各47例。治疗组在常规治疗及BAL基础上加以俯卧位治疗,对照组给予常规治疗及BAL。比较两组患儿发热、肺部体征、住院时间、肺复张和肺功能改善情况。结果治疗组肺部体征改善时间、住院时间短于对照组,住院第7天、出院当天及出院后1周肺复张有效率高于对照组(P0.05)。结论BAL联合俯卧位在儿童MPP伴肺不张治疗中,有利于缩短肺部体征改善时间及住院时间,有利于肺复张和肺功能的改善。

关键词：肺炎支原体肺炎支气管肺泡灌洗俯卧位肺不张儿童

灌耳疗法联合穴位贴敷对原发性痛经患者前列腺素水平的影响

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实用中医内科杂志 2024年第8期38卷 127-130页

作者：乔林静赵娜张飒保定市第一中医院河北保定071000

目的探讨灌耳疗法联合穴位贴敷对原发性痛经患者前列腺素水平的影响。方法选择2019年12月-2020年12月收治的124例原发性痛经患者利用双色球法随机均分为对照组和联合组。对照组实施酒精灌耳治疗,联合组实施灌耳疗法联合穴位贴敷治疗... 详细信息

目的探讨灌耳疗法联合穴位贴敷对原发性痛经患者前列腺素水平的影响。方法选择2019年12月-2020年12月收治的124例原发性痛经患者利用双色球法随机均分为对照组和联合组。对照组实施酒精灌耳治疗,联合组实施灌耳疗法联合穴位贴敷治疗。比较两组临床疗效,治疗前后的疼痛程度、中医证候积分及前列腺素水平。结果比较对照组的77.42%,联合组的总有效率为96.77%,差异具有统计学意义(P<0.05)。两组治疗7、14、21 d后VAS评分均显著下降,比较对照组,联合组VAS评分较低,差异具有统计学意义(P<0.05)。两组治疗后小腹冷痛、畏寒肢冷、舌质黯等中医证候积分均显著下降,比较对照组,联合组中医证候积分降低,差异具有统计学意义(P<0.05)。两组治疗后前列腺素E2(PGE2)、环氧化酶-2(COX-2)水平均显著上升,比较对照组,联合组PGE2、COX-2水平更高;治疗后前列腺素F2α(PGF2α)、环磷酸腺苷(cAMP)均显著下降,比较对照组,联合组PGF2α、cAMP水平较低,差异具有统计学意义(P<0.05)。结论应用灌耳疗法联合穴位贴敷治疗原发性痛经患者效果显著,明显降低疼痛程度和中医证候积分,改善前列腺素水平,值得推广。

关键词：灌耳疗法穴位贴敷原发性痛经前列腺素

Kinetic modeling of high-temperature oxidation of pure Mg

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Journal of Magnesium and Alloys 2020年第3期8卷 819-831页

作者： sa Ma Fangzhou Xing na Ta Lijun Zhang State Key Laboratory of Powder Metallurgy Central South UniversityChangshaHunan 410083P.R.China Max-Planck-Institut fiir Eisenforschung GmbH Max-Planck-Straβe 140237DusseldorfGermany

A variety of experimental tracer diffusivities of Mg and O in magnesium oxide available in the literature were first *** mobilities including bulk and short-circuit diffusion of Mg and O were then obtained by means of the CALPHAD(Calculation of Phase Diagram)***,the diffusion-controlled kinetic model of oxidation in a gas-MgO-Mg environment was developed based on the moving boundary model and Fick's law,coupling with the modified thermodynamic description of MgO.A mathematical expression for parabolic rate constant kp of the oxide scale was derived for magnesia and correlated with the thermodynamic and diffusion kinetic *** evaluated kp results were in line with the experimental ***,the oxidation process of pure magnesium at 673 K was model-predicted,and the predicted evolution of the oxide thicknesses agreed very well with the experimental *** was indicated that the grain boundaries diffusion of magnesium cations predominated the high temperature oxidation process.

关键词： MgO Diffusion Oxidation Atomic mobility CALPHAD Wagner's model

Collisionless tool orientation smoothing above blade stream surface using NURBS envelope

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Journal of Zhejiang University-Science A(Applied Physics & Engineering) 2013年第3期14卷 187-197页

作者： Jing-hua XU Shu-you ZHANG Jian-rong TAN Ri-na sa State Key Laboratory of Fluid Power Transmission and Control Zhejiang University

In five-axis machining,tool orientation above a blade stream surface may lead to tool collision and a decrease in workpiece ***,collisionless tool orientation smoothing(TOS)becomes an important *** the basis of a constant scallop height tool path,the triangular facets in the faces,vertices format are constructed from cutter contact(CC)using the Voronoi incremental *** cutter location(CL)points candidate set is represented by an oblique elliptic cone whose vertex lies at CC using NURBS *** the CL point is above its CC is judged by the dot product between the normal vector and the point on triangulation nearest to the CL *** curvatures at CC are obtained by fitting a moving least square(MLS) quadratic patch to the local neighborhood of a vertex and calculating eigenvectors and eigenvalues of the Hessian *** surface elastic energy is employed as the weight in selection from the NURBS *** collision is judged by NURBS surface *** can then be expressed by selecting a CL point for each CC point and converted into a numerical control(NC)code automatically according to the postprocessor type of the machine *** proposed method is verified by finishing of a cryogenic turboexpander impeller of air separation equipment.

关键词： Tool orientation smoothing(TOS) Blade stream surface Triangular facet NURBS envelope Surface elastic energy

基于无人机和地面调查的典型草原“种-面积”关系

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中国环境科学 2024年第9期44卷 5169-5179页

作者：朱萨宁高吉喜万华伟尤春赫杨雪郭雨桐祝晨佳吕娜中国环境科学研究院生态研究所北京100012 生态环境部卫星环境应用中心北京100094 北京林业大学草业与草原学院北京100091

本研究以呼伦贝尔市典型草原为研究对象,通过传统地面样方调查和无人机调查,系统研究了“种-面积”关系,确定了典型草原地面与无人机调查的最小调查面积.调查结果表明:1)典型草原的物种数量与调查面积呈非线性关系,“种-面积”关系呈现... 详细信息

本研究以呼伦贝尔市典型草原为研究对象,通过传统地面样方调查和无人机调查,系统研究了“种-面积”关系,确定了典型草原地面与无人机调查的最小调查面积.调查结果表明:1)典型草原的物种数量与调查面积呈非线性关系,“种-面积”关系呈现为S=aln(bA+1).基于组合样方法,至少要包括8个1m^(2)小样方,才能达到涵盖80%物种的目标,基于巢式样法,需要的最小调查面积为8.63m^(2);2)针对典型草原,无人机调查的平均物种发现率为传统地面调查方法的81.7%,无人机可作为地面调查的辅助手段,提高调查的效率;3)在典型草原物种监测中,可选取1个或2个组合样方,并使用无人机进行4~5次随机拍摄作为补充,此时可发现典型草原物种总数的84.7%~88.3%.该方法相对传统的3~5次重复样方调查,代表性更高,调查效率更高.

关键词： “种-面积”关系无人机地面调查典型草原