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Identification and characterization of genes related to m^(6)A modification in kiwifruit using RNA-seq and ATAC-seq

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Horticultural Plant Journal 2024年第5期10卷 1121-1133页

作者： xiaoli Hu Tong Li Changbin Xu Yanna Xu Congjun You Xinyi Li Jinli Gong Xiaolong Li Xuepeng sun Collaborative Innovation Center for Efficient and Green Production of Agriculture in Mountainous Areas of Zhejiang Province Key Laboratory of Quality and Safety Control for Subtropical Fruit and VegetableMinistry of Agriculture and Rural AffairsCollege of Horticulture ScienceZhejiang A&F UniversityHangzhouZhejiang 311300China

N6-methyladenosine(m^(6)A)RNA modification is a conserved mechanism that regulates the fate of RNA across eukaryotic *** its significance,a comprehensive analysis of m^(6)A-related genes in non-model plants,such as kiwifruit,is ***,we identified 36 m^(6)A-related genes in the kiwifruit genome according to homology and phylogenetic *** performed bioinformatics and evolutionary analyses of the writer,eraser,and reader families of m^(6)A *** of public RNA-seq data collected from samples under various biotic and abiotic stresses indicated that most m^(6)A-related genes were remarkably expressed under different *** construction of gene co-expression networks,we found significant correlations between several m^(6)A-related genes and transcription factors(TFs)as well as receptor-like genes during the development and ripening of ***,we performed ATAC-seq assays on diverse kiwifruit tissues to investigate the regulatory mechanisms of m^(6)A-related *** identified 10 common open chromatin regions that were present in at least two tissues,and these regions might serve as potential binding sites for MADS protein,C2H2 protein,and other predicted *** study offers comprehensive insights into the gene family of m^(6)A-related components in kiwifruit,which will lay foundation for exploring mechanisms of post-transcriptional regulation involved in development and adaptation of kiwifruit.

关键词： Kiwifruit N^(6)-methyladenosine Phylogenetic analysis cis-element Co-expression network

Multipair two-way massive MIMO AF relaying with ZFR/ZFT and hardware impairments over high-altitude platforms

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Journal of Communications and Information Networks 2016年第3期1卷 105-114页

作者： sun xiaoli XU Kui XU Youyun MA Wenfeng PLA University of Science and Technology Nanjing 210007China Nanjing University of Posts and Telecommunications Nanjing 210023China

HAPs(High-Altitude Platforms)are one of the most promising alternative infrastructures for providing wireless communications to overcome the shortcomings of terrestrial-tower-based and satellite *** paper investigates a multipair two-way MM-AF(Massive MIMO Amplify-and-Forward)relay system over HAPs,where multiple user pairs exchange information within a pair through a relay with a very large number of antennas,and a HAP channel is modeled to follow a Rician fading distribution because of the presence of a line-of-sight ***,the effect of hardware impairments on a multipair two-way MM-AF system is taken into consideration and is modeled using transmit and receive distortion ***,ZFR/ZFT(Zero-Forcing Reception/Zero-Forcing Transmission)processing matrices of HDR(Half-Duplex Relaying)and FDR(Full-Duplex Relaying)with imperfect channel state information are ***,the asymptotic expressions of an end-to-end SINR are *** analyses and simulation results show that when the number of relay antennas grows very large,the SE(Spectral Efficiency)of an MM-AF relay system is limited by hardware impairments at the users instead of those at the relay,or by other types of ***,the quality of the hardware at a relay can be decreased without significantly degrading performance.

关键词： massive MIMO half-duplex full-duplex Rician fading channels hardware impairments

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肯尼亚私立高等教育的发展和挑战

教育发展研究 2007年第9B期27卷 73-76页

作者：孙小丽浙江师范大学非洲教育研究中心硕士研究生金华321004

20世纪80年代以来,非洲的高等教育危机引发了私立高等教育的发展,肯尼亚是一个典型的代表。肯尼亚私立高等教育历史虽然不长,但发展速度迅猛,已经成为高等教育的重要组成部分。本文立足肯尼亚的现实,分析了该国私立高等教育发展的原因,... 详细信息

20世纪80年代以来,非洲的高等教育危机引发了私立高等教育的发展,肯尼亚是一个典型的代表。肯尼亚私立高等教育历史虽然不长,但发展速度迅猛,已经成为高等教育的重要组成部分。本文立足肯尼亚的现实,分析了该国私立高等教育发展的原因,介绍了其发展现状和私立高校的特色,最后从持续发展的角度提出了私立高等教育面临的挑战,意在全方位地展现肯尼亚私立高等教育发展的概貌。

关键词：肯尼亚私立高等教育私立高校

Protein crystallization with DNA templates

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Particuology 2024年第3期86卷 262-268页

作者： Bo Zhang Jia Yu Zhe Pu Wenhui Guo Ruoqi Yang Yu Wen Zhijiang Rong Hui Wang xiaoli Wu Bo Wang Hongyan sun School of Environment and Resources Taiyuan University of Science and TechnologyTaiyuan030024China School of Chemical Engineering and Technology Taiyuan University of Science and TechnologyTaiyuan030024China

Protein crystallization plays a significant role in three-dimensional structural analysis and protein *** is important to increase the crystallization efficiency,which is possible by adding hetero-geneous templates in crystallization *** is biologically compatible and artificially designable polymer,which is easy to *** this study,single-and double-stranded DNA of precise sequences were designed and used as templates to promote protein crystallization of lysozyme and ***-fluence of DNA,single-stranded DNA with 10,20,40 bases and double-stranded DNA with 10,20,40 base pairs,were *** success rate of obtaining crystals of lysozyme and catalase in equal period was significantly improved with the addition of DNA comparing without templates ***-stranded DNA led to higher nucleation rate than that with single-stranded *** promotion of nucleation was more obvious at low concentration of protein solution and with longer chain DNA *** number and crystallization rate was enhanced with addition of long double-stranded DNA *** the results confirm that DNA is an effective polymer additive to enhance protein crystallization,especially for the application of the scarce protein crystallization.

关键词： DNA Polymer Additive Protein Crystallization

Use of Qishen granule for the treatment of heart failure: A systematic review and meta-analysis of animal studies

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Journal of Traditional Chinese Medical Sciences 2020年第2期7卷 121-132页

作者： Junjie Liu Shuangjie Yang Wei Wang Yili Zhang Qiyan Wang xiaoli sun Nannan Tan Kangjia Du Yong Wang Huihui Zhao Dongzhimen Hospital Beijing University of Chinese MedicineBeijing100700China School of Traditional Chinese Medicine Beijing University of Chinese MedicineBeijing102488China School of Life Sciences Beijing University of Chinese MedicineBeijing102488China

Objective:To evaluate the effect of Qishen granule(QSG)on heart failure(HF)and the potential mechanisms involved in animal ***:Studies of QSG in animals with HF were identified by searches of eight databases up to August 1,*** authors independently reviewed each study and the Collaborative Approach to Metaanalysis and Review of Animal Data from Experimental Studies 10-item checklist was used for quality *** primary outcomes were indicators of left ventricular ejection fraction and/or left ventricular fractional *** secondary outcome measures were ventricular structure,hemodynamics,myocardial injury-related indicators,and the mechanisms whereby QSG ameliorates HF.A metaanalysis was performed and all the data were analyzed using RevMan 5.3 ***:Thirteen studies containing a total of 240 animals met the criteria for the *** quality score of the studies ranged from three to six *** meta-analysis showed a significant effect of QSG to improve cardiac function,inhibit ventricular remodeling,improve circulation,and attenuate myocardial *** possible mechanisms was identified to be suppression of the renin-angiotensinaldosterone system,which has anti-fibrotic,anti-apoptotic,anti-inflammatory,and anti-oxidative effects in the myocardium,the prevention of calcium overload,and the promotion of myocardial energy ***:Our review shows that QSG has a cardioprotective effect in animal models of ***,due to the relatively poor quality of the included studies and the differences between humans and animal models,the results should be interpreted with caution.

关键词： Qishen granule Heart failure Systematic review Animal model Traditional Chinese medicine

Regulation of AtbZIP1 Gene Expression by T-DNA Insertion in Arabidopsis thaliana

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Journal of Northeast Agricultural University(English Edition) 2010年第3期17卷 19-23页

作者： sun xiaoli SHAO Wanchen CAI Hua ZHU Yanming College of Life Sciences Northeast Agricultural University Harbin 150030 China

bZIP transcription factor family is one of the largest groups of the plant transcription factor families and plays an important role in plant growth and adaption to the abiotic stresses. In this study, two AtbZIP1 mutant Arabidopsis （bzipl） were used with T-DNA inserted into two different sites, designated as SALK-556773 and SALK-660942, in order to identify different effects on AtbZIP1 gene expression by different T-DNA insertion sites. PCR and RT-PCR results revealed that T-DNA insertion in CDS region could effectively inhibit AtbZIP1 gene expression, while T-DNA insertion in 3＇-UTR couldn＇t. The phenotype analysis further confirmed the differences and showed that T-DNA insertion in CDS region decreased plants＇ drought resistance, while in 3＇-UTR couldn＇t. The phenotype assays also suggested that AtbZIP1 held pivotal roles in plant response to drought stress.

关键词： T-DNA insertion AtbZIP1 different sites drought stress Arabidopsis thaliana

Neuroprotective effect of Longshengzhi capsule following permanent middle cerebral artery occlusion in rats

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Journal of Traditional Chinese Medical Sciences 2020年第1期7卷 12-19页

作者： xiaoli sun Shuzhen Guo Yong Wang Bangze Fu Junjie Liu Yili Zhang Ruocong Yang Chun Li Jian Gao Li Gao Wei Wang School of Traditional Chinese Medicine Beijing University of Chinese MedicineBeijing100029China School of Life Science Beijing University of Chinese MedicineBeijing100029China School of Biomedicine Beijing City UniversityBeijing100083China Dongzhimen Hospital Beijing University of Chinese MedicineBeijing100029China School of Chinese Materia Medica Beijing University of Chinese MedicineBeijing100029China

Objective:Longshengzhi capsule(LSZC)is an optimized preparation based on the traditional Chinese Medicine formula Buyanghuanwu Decoction(BYHWD),and is approved by the China Food and Drug Administration for treating stroke-induced disability and vascular ***,we examined the pharmacodynamics,anti-apoptotic and anti-oxidant actions,and potential mechanisms of action of LSZC following stroke in ***:Permanent middle cerebral artery occlusion(MCAO)was used as an ischemic stroke *** was administered *** examined the survival rate,bodyweight,and neurological deficits in stroke *** infarct size was determined by 2,3,5-triphenyltetrazolium chloride *** pathology was examined using hematoxylin and eosin staining,Nissl staining,and TdT-mediated dUTP Nick-End Labeling ***,catalase,superoxide dismutase,and glutathione levels were examined by commercial *** of Nrf2,heme oxygenase-1,Bax,Bcl-2,cleaved caspase-3,and caspase-3 proteins in brain tissue was measured by Western ***:LSZC markedly improved the survival rate and bodyweight,and reduced infarct volume and neurological deficit scores,in MCAO stroke *** also significantly attenuated oxidative stress,as indicated by decreased expression of malondialdehyde,and upregulation of Nrf2,heme oxygenase-1,catalase,superoxide dismutase,and ***,LSZC significantly decreased apoptosis,including a decrease in Bax and cleaved caspase-3 expression,and an increase in Bcl-2,as well as a reduction in numbers of apoptotic ***:LSZC treatment is neuroprotective against ischemic stroke,potentially via reducing oxidative stress and *** Nrf2 and apoptotic signaling pathways may play important roles in the antioxidant and anti-apoptotic actions of LSZC.

关键词： Longshengzhi capsule Middle cerebral artery occlusion Oxidative stress Apoptosis Cerebral protective

A Glycine soja methionine sulfoxide reductase B5a interacts with the Ca2+/CAM-binding kinase GsCBRLK and activates ROS signaling under carbonate alkaline stress

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A Glycine soja methionine sulfoxide reductase B5a interacts ...

注射用卡瑞利珠单抗致免疫相关性心肌炎的文献病例分析

2016年全国植物生物学大会

作者： xiaoli sun Mingzhe sun Bowei Jia Yanming Zhu Crop Stress Molecular Biology Laboratory Heilongjiang Bayi Agricultural University

Although researches have extensively illustrated the molecular basis of plant responses to salt and high p H stresses,the knowledge on carbonate alkaline stress is poor and the specific responsive mechanism remains elusive. We have previously characterized a Glycine soja Ca2+/CAM-dependent kinase GsCBRLK,which could increase salt tolerance. Here,we characterized a methionine sulfoxide reductase B protein GsMSRB5 a as a GsCBRLK interactor by using Y2 H and Bi Fc assays. Further analyses showed that the N-terminal variable domain of GsCBRLK contributed to GsMSRB5 a interaction. Y2 H assays also revealed the interaction specificity of GsCBRLK with wild soybean MSRB subfamily proteins,and determined that the Box I/Box II-containing regions within GsMSRBs were responsible for their interaction. Furthermore,we also illustrated that the N-terminal basic regions in GsMSRBs functioned as transit peptides,which targeted themselves into chloroplasts and thereby prevented their interaction with GsCBRLK. Nevertheless,deletion of these regions allowed them to localize on PM and interact with GsCBRLK. In addition,we also showed that GsMSRB5 a and GsCBRLK displayed overlapping tissue expression specificity and coincident expression patterns under carbonate alkaline stress. Phenotypic experiments demonstrated that GsMSRB5 a and GsCBRLK overexpression in Arabidopsis enhanced carbonate alkaline stress tolerance. Further investigations elucidated that GsMSRB5 a and GsCBRLK inhibited ROS accumulation by modifying expression of ROS signaling,biosynthesis and scavenging genes. Summarily,our results demonstrated that GsCBRLK and GsMSRB5 a interacted with each other,and activated ROS signaling under carbonate alkaline stress.

关键词： carbonate alkaline stress methionine sulfoxide reductase Ca2+/CAM- binding kinase ROS signaling Glycine soja Arabidopsis

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中国医院用药评价与分析 2024年第9期24卷 1149-1152页

作者：孙晓利白万军刘红冯晓杰王紫月梁平冯锐霍丽曼河北医科大学第四医院药学部石家庄050011 河北省人民医院药学部石家庄050051

目的:了解注射用卡瑞利珠单抗致免疫相关性心肌炎的临床特点、治疗与转归,促进临床安全用药。方法:收集建库至2024年7月Wiley Online Library、Embase、PubMed、中国生物医学文献数据库、万方数据库、中国知网、维普数据库等数据库中收... 详细信息

目的:了解注射用卡瑞利珠单抗致免疫相关性心肌炎的临床特点、治疗与转归,促进临床安全用药。方法:收集建库至2024年7月Wiley Online Library、Embase、PubMed、中国生物医学文献数据库、万方数据库、中国知网、维普数据库等数据库中收录的注射用卡瑞利珠单抗致免疫相关性心肌炎的病例报道文献。对纳入文献中患者的基本信息、用药方案及联合用药、合并症、心肌炎发生时间、转归与干预等进行统计分析。结果:共纳入25篇文献,涉及26例患者,其中男性患者14例,女性患者12例;年龄分布于45~79岁,平均年龄为64.7岁;临床诊断以肺癌为主,共9例;记录有既往病史的有9例,其中糖尿病史3例;记录有联合用药情况的有21例;11例患者发生心肌炎的时间为注射用卡瑞利珠单抗第1个周期用药后,最早为首次用药后2 d;最常见的临床表现包括胸闷、乏力、呼吸困难、肌肉无力或酸痛等,15例患者同时合并其他免疫相关不良反应;19例患者好转,3例未好转,4例死亡。出现心肌炎时,所有患者均及时给予了糖皮质激素,2例植入了永久心脏起搏器。结论:临床在使用注射用卡瑞利珠单抗时,应做好常规监测和心脏功能相关基线检查,治疗期间应密切观察,一旦出现心肌炎症状,要尽早采取干预措施,保证患者用药安全。

关键词：注射用卡瑞利珠单抗不良反应免疫相关性心肌炎

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