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Combination of p-Coumaric acid and lactoferrin ameliorates cognitive deficits through suppressing C/EBPβ/AEP pathway and modulating intestinal flora in APP/PS1 mice

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Food Science and Human Wellness 2024年

作者： Yan Zhou Yi-Tian sun Hui guo Yan-Zi Liang Si-Yuan Zhu Yu Sha Zhong-Xiao Wan {2.-Ying Xu {2.n-Rong {2.ng Li-Qiang qin Yun-Hong Li Department of Nutrition and Food Hygiene， School of Public Health， jiangsu key {2. of Preventive and Translational Medicine for Geriatric Diseases， MOE key {2. of Geriatric Diseases and Immunology， Suzhou Medical college of Soochow university Department of Medical Technology， Suzhou Vocational Health College state key {2. of Radiation Medicine and Protection， School of Radiation Medicine and Protection， Suzhou Medical college of Soochow university Suzhou Municipal Center for Disease control and Prevention

Alzheimer's disease （AD） has become an important public health issue worldwide. P-Coumaric acid （CA） and lactoferrin （Lf） possessed antioxidant， anti-inflammatory， and anti-AD activities. Herein， we hypothesized that a combination treatment of CA and Lf would synergistically improve AD symptoms and studied its mechanisms. Twelve-week-old APP/PS1 mice were treated with CA， Lf， or both CA and Lf for 8 weeks. Results showed that individual and combined treatments could ameliorate cognitive deficits to varying degrees， with stronger effects for combined intervention than for CA or Lf alone. Specifically， combined treatment was most effective in improving nesting ability and reducing Aβ1-42.eposition in mice. Moreover， combined treatment was more effective in suppressing APP， BACE1， and inflammation; it inhibited the CCAAT-enhancer-binding protein （C/EBPβ）/asparagine endopeptidase （AEP） signaling pathway in the hippocampus and colon and upregulated the expression of tight junction protein ZO-1 in the colon; it also decreased the F/B ratio at the phylum level and increased the relative abundance of Prevotellaceae， Lachnospira， and Eubacterium at the genus level. Overall， the combination of CA and Lf may ameliorate cognitive deficits in APP/PS1 mice by improving inflammation and inhibiting the C/EBPβ/AEP signaling pathway via modulating gut microbiome.

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The Study on Produce and Properties ofF-Heparin Surface Modified IOLs

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Chinese Journal of Biomedical Engineering(English Edition) 2003年第1期12卷 1-16页

作者： GU Han qing 1,YUAN jia qin 2.sun Hui min 2.ZHANG Hai wen 1,LU Mo gu 1,WANG Gui qin 3,guo Hong yu 3,XU Yan shan 2.his work is funded in part by the National 10th Five Year Plan on Capture key of Science and Technolog (2.01 BA706B17) 1 Tianjin Institute of Urological Surgery,Tianjin 3002.1,china,2.Tianjin Medical university Eye Center,Tianjin 300070,china 3 Tianjin Medical university,Tiangjin 300070,china

Objective:To study the biocompatibility of F heparin surface modified IOLs (F HSM IOLs) and provide theoretical and experimental basis for clinical use and new IOL development. Methods The technique of carrier energy ion beam combined with low temperature and low pressure plasma was used to modify the surface of PMMA IOLs. The physicochemical and optical properties of F HSM IOLs were examined. In vitro, the cultured cells adherence and growth on IOLs were studied. In vivo, F HSM IOLs were implanted into rabbits and Rhesus Monkeys eyes. The biocompatibility of F HSM IOLs, PMMA IOLs and HSM IOLs was compared. Results There were fewer adhered cells on the surface of F HSM IOLs with no toxicity to the cultured fibroblasts. F HSM IOLs caused less inflammatory reaction, broke BAB more slightly and decreased posterior capsular opacity. F HSM IOLs possessed better biocompatiblity. Our research also suggested that the brownish F HSM IOLs could prevent the short wave length light and ultraviolet rays from injuring the retina. Conclusion F HSM IOLs possessed better biocompatibility than conventional PMMA IOLs and can be used in clinical works after further inspection and approval.

关键词： F heparin surface modified IOLs Biocompatibility Preparation In vitro experiments In vivo experiments Rabbits Rhesus Monkeys

Brain mechanism of acupuncture for children with anisometropic amblyopia: a resting functional magnetic resonance imaging study based on voxel-mirrored homotopic connectivity

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International Journal of Ophthalmology(English edition) 2024年第2期17卷 339-347页

作者： Jue Wang Jing jia Yan sun Chong-Bing Ma Yu-Zhu Chen An-{2. Liu Xing-Ke Yan College of Acupuncture-Moxibustion and Tuina Gansu University of Chinese MedicineLanzhou 730101Gansu ProvinceChina Department of Ophthalmology Lanzhou Purui Ophthalmology HospitalLanzhou 730050Gansu ProvinceChina

AIM:To explore the brain mechanism of acupuncture for children with anisometropic amblyopia using the voxelmirror homotopic connectivity(VMHC)analysis method of resting functional magnetic resonance imaging(rs-fMRI)technology based on clinical ***:Eighty children with anisometropic monocular amblyopia were randomly divided into two groups:control(40 cases,1 case of shedding)and acupuncture(40 cases,1 case of shedding)*** control group was treated with glasses,red flash,grating,and visual stimulations,with each procedure conducted for 5{2. per *** on routine treatment,the acupuncture group underwent acupuncture of“regulating qi and unblocking meridians to bright eyes”,Jing{2.g(BL1),Cuanzhu(BL2.,Guang{2.g(GB37),Fengchi(GB2.)acupoints were taken on both sides,with the needle kept for 30{2. each *** groups were treated once every other day,three times per week,for a total of *** the treatment,the overall curative effect of the two groups and the latency and amplitude changes of P100 wave of pattern visual-evoked potential were *** the same time,nine children with left eye amblyopia were randomly selected from the two groups and were scanned with rsfMRI before and after *** differences in the brain regions between the two groups were compared and analyzed with ***:Chi-square test showed a notable difference in the total efficiency rate between the acupuncture(94.87%)and control groups(79.49%).Regarding the P100 wave latency and amplitude,the acupuncture group had significantly shorter latency and higher amplitude of P100 wave than the control ***,the VMHC values of the bilateral temporal lobe,superior temporal gyrus,and middle temporal gyrus were notably increased in the acupuncture group after ***:Acupuncture combined with conventional treatment can significantly improve the corrected visual acuity and optic nerve conduction in children with anisometropic ***

关键词： resting functional magnetic resonance imaging voxel-mirror homotopy connection anisometropic amblyopia acupuncture

白皮松溃疡病菌的鉴定、生物学特性及其对不同杀菌剂的敏感性研究

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西北林学院学报 2024年第3期39卷 18-25,66页

作者：孙艳焦嘉卉李果宏郭童童丁俊园郭梦松唐光辉李培琴西北农林科技大学林学院、西部森林生物灾害治理国家林业和草原局重点实验室陕西杨陵712100

近年来,白皮松溃疡病在陕西省大量发生,严重影响了白皮松的经济和生态价值。为明确白皮松溃疡病的病原,通过组织分离法和柯赫氏法则致病性验证,分离获得白皮松溃疡病菌。通过组织切片观察、形态学特征及ITS-RPB2.β-tubulin序列的串联分... 详细信息

近年来,白皮松溃疡病在陕西省大量发生,严重影响了白皮松的经济和生态价值。为明确白皮松溃疡病的病原,通过组织分离法和柯赫氏法则致病性验证,分离获得白皮松溃疡病菌。通过组织切片观察、形态学特征及ITS-RPB2.β-tubulin序列的串联分析,将白皮松溃疡病菌鉴定为子囊菌门真菌Nothophoma quercina。该病原真菌在胡萝卜固体培养基上生长最快,最适碳源为淀粉,最适氮源为蛋白胨和牛肉浸膏,最适培养温度为2.℃,最适pH为5~8。杀菌剂室内毒力测定结果表明,白皮松溃疡病菌对9种供试杀菌剂表现出不同的敏感性,除甲霜灵外,该病菌对化学杀菌剂的敏感性显著强于生物源杀菌剂,在化学杀菌剂中,白皮松溃疡病菌对苯醚甲环唑最敏感,EC 50仅为0.108 mg·L^(-1),其次分别为已唑醇、嘧菌酯和多菌灵,其相应的EC 50分别为0.2.2.0.62. mg·L^(-1)和0.62. mg·L^(-1);生物源杀菌剂中,该病菌对小檗碱和蛇床子素较敏感,其相应的EC 50分别为11.914 mg·L^(-1)和17.375 mg·L^(-1)。研究结果能为白皮松溃疡病的有效防控提供指导依据。

关键词：白皮松溃疡病形态鉴定分子鉴定 Nothophoma 敏感性测定

基于UHPLC Q-Exactive Plus Orbitrap HRMS高分辨质谱技术的隔山消提取物化学成分分析

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中国新药杂志 2024年第5期33卷 489-498页

作者：勾健吴宗芹孟欣韦晓毅刘春花朱迪秦兰孙佳贵州医科大学贵州省药物制剂重点实验室/省部共建药用植物功效与利用国家重点实验室贵阳550001 贵州医科大学民族药与中药开发应用教育部工程研究中心贵阳550001 贵州医科大学药学院贵阳550001 红云制药(贵州)有限公司凯里556000 贵州中医药大学第一附属医院贵阳550001

目的:采用UHPLC Q-Exactive Plus Orbitrap高分辨质谱(HRMS)技术建立全面、快速分析隔山消化学成分的方法,为隔山消的药效物质研究提供科学依据。方法:采用Hypersi 1 GOLD色谱柱(100 mm×2.1 mm,1.9μm),以0.1%甲酸水溶液(A)-0.1%甲酸... 详细信息

目的:采用UHPLC Q-Exactive Plus Orbitrap高分辨质谱(HRMS)技术建立全面、快速分析隔山消化学成分的方法,为隔山消的药效物质研究提供科学依据。方法:采用Hypersi 1 GOLD色谱柱(100 mm×2.1 mm,1.9μm),以0.1%甲酸水溶液(A)-0.1%甲酸乙腈溶液(B)为流动相进行梯度洗脱,流速0.3 mL·min^(-1),柱温40℃。在电喷雾离子源正、负离子模式下采集数据,扫描范围m/z 100~1500 Da;喷雾电压3.5 kV(+),2.5 kV(-);鞘气流速35 arb;辅助气流速10 arb;毛细管温度32.℃;辅助加热器温度350℃。依据HRMS提供的准分子离子峰及碎片离子的精确分子质量信息,结合对照品比对,相关文献报道及m/z Cloud和PUBchem数据库比对二级质谱进行鉴定。结果:从隔山消提取物中共分析出66个化合物,包括C2.甾体类34个,有机酸类13个,苯乙酮类5个,香豆素类4个,生物碱类4个和其他类6个化合物,其中18个化合物为首次在隔山消中报道。结论:该方法可对隔山消化学成分进行快速、准确、全面的定性分析,为今后进一步阐明其药效物质基础和质量控制提供科学依据。

关键词： UHPLC Q-Exactive Plus Orbitrap HRMS 隔山消化学成分裂解规律

Emerging contaminants:A One Health perspective

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The Innovation 2024年第4期5卷 140-170,139页

作者： Fang Wang Leilei Xiang Kelvin Sze-Yin Leung Martin Elsner Ying Zhang Yuming guo Bo Pan Hongwen {2. Taicheng An Guangguo Ying Bryan WBrooks Deyi Hou Damian EHelbling jianqiang {2. Hao Qiu Timothy MVogel Wei Zhang Yanzheng Gao Myrna JSimpson Yi Luo Scott XChang Guanyong Su Bryan MWong Tzung-May Fu dong Zhu Karl JJobst Chengjun Ge Frederic Coulon Jean Damascene Harindintwali Xiankui Zeng Haijun Wang Yuhao Fu Zhong Wei Rainer Lohmann Changer Chen Yang Song Concepcion Sanchez-Cid Yu Wang Ali El-Naggar Yiming Yao Yanran Huang Japhet Cheuk-Fung Law Chenggang Gu Huizhong Shen Yanpeng Gao Chao qin Hao Li Tong Zhang Natàlia Corcoll min Liu Daniel SAlessi Hui Li Kristian KBrandt Yolanda Pico Cheng Gu jianhua guo jianqiang Su Philippe Corvini Mao Ye Teresa Rocha-Santos Huan He Yi Yang Meiping Tong Weina Zhang Fidèle Suanon Ferdi Brahushi Zhenyu Wang Syed AHashsham Marko Virta qingbin Yuan Gaofei jiang Louis A.Tremblay qingwei Bu Jichun Wu Willie Peijnenburg Edward Topp Xinde Cao Xin jiang minghui Zheng Taolin Zhang yongming Luo Lizhong Zhu Xiangdong Li DamiàBarceló jianmin Chen Baoshan Xing Wulf Amelung Zongwei Cai Ravi Naidu Qirong Shen Janusz Pawliszyn yong-guan Zhu Andreas Schaeffer Matthias C.Rillig Fengchang Wu Gang Yu James M.Tiedje state key {2. of Soil and Sustainable Agriculture Institute of Soil ScienceChinese Academy of SciencesNanjing 210008China university of Chinese Academy of Sciences Beijing 100049China Department of Chemistry Hong Kong Baptist UniversityHong KongChina HKBU Institute of Research and Continuing Education Shenzhen Virtual University ParkShenzhenChina Technical university of Munich TUM School of Natural SciencesInstitute of Hydrochemistry85748 GarchingGermany School of Resources&Environment Northeast Agricultural UniversityHarbin 150030China Climate Air Quality Research UnitSchool of Public Health and Preventive MedicineMonash UniversityMelbourneVICAustralia Faculty of Environmental Science&Engineering Kunming University of Science&TechnologyKunming 650500China ministry of Education key {2. of Pollution Processes and Environmental Criteria College of Environmental Science and EngineeringNankai UniversityTianjin 300350China Guangdong key {2. of Environmental Catalysis and Health Risk control School of Environmental Science and EngineeringInstitute of Environmental Health and Pollution ControlGuangdong University of TechnologyGuangzhou 510006China ministry of Education key {2. of Environmental Theoretical Chemistry South China Normal UniversityGuangzhouGuangdong 510006China Department of Environmental Science Baylor UniversityWacoTXUSA Center for Reservoir and Aquatic Systems Research(CRASR) Baylor UniversityWacoTXUSA School of Environment Tsinghua UniversityBeijing 100084China School of Civil and Environmental Engineering Cornell UniversityIthacaNY 14853USA key laboratory of Microbial Technology for Industrial Pollution {2. of Zhejiang Province Zhejiang University of TechnologyHangzhou 310014China School of Environmental Science and Engineering Shanghai Jiao Tong UniversityShanghai 200240China Laboratoire d’Ecologie Microbienne Universite Claude Bernard Lyon 1UMR CNRS 5557UMR INRAE 1418VetAgro Sup69622 VilleurbanneFrance Department of Pla

Environmental pollution is escalating due to rapid global development that often prioritizes human needs over planetary *** global efforts to mitigate legacy pollutants,the continuous introduction of new substances remains a major threat to both people and the *** response,global initiatives are focusing on risk assessment and regulation of emerging contaminants,as demonstrated by the ongoing efforts to establish the UN’s Intergovernmental Science-Policy Panel on Chemicals,Waste,and Pollution *** review identifies the sources and impacts of emerging contaminants on planetary health,emphasizing the importance of adopting a One Health *** for monitoring and addressing these pollutants are discussed,underscoring the need for robust and socially equitable environmental policies at both regional and international *** actions are needed to transition toward sustainable pollution management practices to safeguard our planet for future generations.

关键词： pollution planet contaminants

尼古丁上调Cx43促发PASMCs炎性反应致小鼠肺动脉重构的机制

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环境卫生学杂志 2024年第9期14卷 780-787页

作者：赵旭侯晓敏孙琳徐祎董霖江雪露饶国娇邹佳佳施熠炜秦小江山西医科大学公共卫生学院太原030001 山西医科大学基础医学院环境暴露血管疾病研究所山西医科大学山西医科大学医学科学院山西医科大学第一医院

目的研究缝隙连接蛋白43(connexin43,Cx43)介导肺动脉平滑肌细胞(pulmonary artery smooth muscle cells,PASMCs)炎性反应在尼古丁致小鼠肺动脉重构中的作用机制。方法5周龄雄性C57BL/6J小鼠、Tagln-Cre(+);Cx43^(flox/WT)小鼠各32.,分别随机分为对照组(灭菌注射水)、0.02.0.2.2.0 mg/(kg·d)尼古丁组,每组8只。小鼠按体重每天固定时间进行一次鼻腔滴注,持续8周。染毒结束后,通过HE染色观察各组小鼠肺动脉重构情况;采用磁性分离法培养C57BL/6J、Tagln-Cre(+);Cx43^(flox/WT)小鼠原代远端PASMCs;通过Western blot法检测小鼠PASMCs中Cx43、肿瘤坏死因子-α(tumor necrosis factor-alpha,TNF-α)、白细胞介素-1β(interleukin 1β,IL-1β)和白细胞介素-6(interleukin 6,IL-6)蛋白表达水平。结果与C57BL/6J小鼠对照组相比,尼古丁染毒组C57BL/6J小鼠体重总体呈现增长趋势,且随着尼古丁浓度增加,体重增长变缓。小鼠远端肺动脉出现管壁增厚、管腔狭窄等典型的动脉重构病理特征,Cx43表达上调且呈剂量依赖性关系。Tagln-Cre(+);Cx43^(flox/WT)小鼠HE染色结果显示其肺动脉管壁增厚和管腔狭窄现象有所缓解,且PASMCs中上述炎性反应因子表达减少。结论Cx43表达下调可缓解尼古丁诱导的小鼠远端PASMCs炎性反应,从而改善小鼠肺动脉重构。

关键词：尼古丁连接蛋白43 肺动脉重构小鼠原代肺动脉平滑肌细胞炎性反应

Responses of rice growth to copper stress under free-air CO_2.enrichment (FACE)

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Chinese Science Bulletin 2007年第19期52卷 2636-2641页

作者： jia HaiXia guo HongYan YIN Ying WANG Qiang {2. qin WANG XiaoRong ZHU jianguo state key {2. of Pollution control and Resources Reuse School of Environment Nanjing University Nanjing 210093 China state key {2. of Soil and Sustainable Agriculture Institute of Soil Science Chinese Academy of Sciences Nanjing 210008 China

The responses of rice to the second degree contamination of copper were studied by pot experiments under free-air CO2.enrichment (FACE) with 570 μmol·mol-1 of CO2. The results showed that the content of copper in rice leaves was reduced with the CO2.concentration reaching 570 μmol·mol-1 and this happened more significantly under the second degree contamination of copper. Under FACE, activities of superoxide dismutase (SOD) enzyme in rice leaves treated by copper contamination were induced, whereas the contents of glutathione (GSH) and glutathione disulfide (GSSG) had no significant difference from the control. In the presence of ambient CO2. activities of SOD enzyme treated by copper pollution were suppressed during the whole rice growth, however, the contents of GSH and GSSG were induced at tillering and jointing stages, and then restored to the control levels in later growth under the second degree contamination of copper. With the rice growing, the content of malondialdehyde (MDA) rises continuously, but there had been no significant difference between the treatments at the same growth stage. Further studies are needed on the response mechanism of rice to Cu stress under elevated CO2.

关键词：铜压力大米生长状态 CO2.缩油污染

Preparation and development of equine hyperimmune globulin F（ab'）2.against severe acute respiratory syndrome coronavirus

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Acta Pharmacologica Sinica 2005年第12期26卷 1479-1484页

作者： jia-hai LU~(2.8,9) Zhong-min {2.~(2.9) Wen-yu HAN~(3,9) {2.-ling WANG~(2.9) Ding-mei ZHANG~2.Yi-fei WANG~4 Sheng-yun sun~4 qin-he YANG~4 Huan-ying ZHENG~5 Bing L WONG~6 Nan-shan ZHONG~7 2.sun Yat-sen university,Guangzhou 510080,china 3 The Agriculture School of Jilin university,Changchun 130062.china 4 Ji-nan university,Guangzhou 510632.china 5 Center for Disease control and Prevention of Guangdong Province,Guangzhou 510300,china 5 Advantek Biologics,Hong Kong,china 7 Guangzhou Institute of Respiratory Diseases,Guangzhou Medical College,Guangzhou 51012.,china sun Yat-sen university Guangzhou 510080 China. jiahailu@***

Aim:The resurgence of severe acute respiratory syndrome(SARS)is still a threatbecause the causative agent remaining in animal reservoirs is not fully understood,and sporadic cases continue to be *** high titers of anti-SARShyperimmune globulin to provide an alternative pathway for emergent future pre-vention and treatment of ***:SARS coronavims(CoV)F69(AY313906)and Z2.Y3(AY394989)were isolated and identified from 2.different Cantoneseonset SARS *** was prepared from SARS-CoV F69 *** horses were immunized 4 times and serum was collected periodically tomeasure the profile of specific IgG and neutralizing antibodies using indirect en-zyme-linked immunosorbent assay and a microneutralization *** were col-lected in large amounts at the peak,where IgG was precipitated using ammoniumsulphate and subsequently digested with *** product was then purifiedusing anion-exchange chromatography to obtain F(ab′)2.fragments,Results:Thespecific IgG and neutralizing antibody titers peaked at approximately week 7 afterthe first immunization,with a maximum value of 1:*** sera collected at thepeak were then *** of approximately 15 g F(ab′)2.was obtained from1 litre antiserum and the purity was above 90% with the titer of 1:512.,which couldneutralize the other strain(SARS-CoV Z2.Y3)as ***:This researchprovides a viable strategy for the prevention and treatment of SARS coronavirusinfection with equine hyperimmune globulin,with the purpose of combating anyresurgence of SARS.

关键词： severe acute respiratory syndrome coronavirus neutralizing antibodies hyperimmune globulin cross protection F(ab’)2.fragments