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TLR4 signaling induced TLR2 expression in the process of mimic cerebral ischemia/reperfusion in vitro

中国科学：生命科学英文版 2010年第2期53卷 223-228页

作者： sun peng1,zhang qing2,han jiyuan1,tian yuan3 & zhang jinghui3 1department of emergency,union hospital,tongji medical college,huazhong university of science and technology,wuhan 430022.china 2department of Anesthesia,union hospital,tongji medical college,huazhong university of science and technology,wuhan 430022.china 3department of General Surgery,union hospital,tongji medical college,huazhong university of science and technology,wuhan 430022.china 1. department of emergency Union Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan 430022 China2. Department of Anesthesia Union Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan 430022 China3. Department of General Surgery Union Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan 430022 China

Both TLR4 and TLR2 participated in the mediation of the inflammatory injury in the process of partial cerebral ischemia/***,it still remains unclear whether a crosstalk exists between TLR2 and TLR4 in ischemic cerebral *** the present study,we investigated the effect of TLR4 signaling on TLR2 expression during mimic cerebral I/R in ***-2 cells were cultured and treated with ischemia/reperfusion,then transfected with the plasmid pEGFP-H1/TLR4-siRNA,the plasmid pEGFP-H1/control sequence-siRNA and the blank plasmid,***,the expression of TLR2 and TLR4 mRNA and protein,NF-κB p65 mRNA and supernatant TNF-α level were significantly higher in ischemia/reperfusion treated cells than those lack of ischemia/reperfusion treatment,and as compared with those in ischemia/reperfusion treated cells without transfection,no significant differences about the above mentioned gene and protein expression were found in the blank plasmid tranfected cells and the plasmid pEGFP-H1/control sequence-siRNA transfected cells respectively,while the expression levels in the plasmid pEGFP-H1/TLR4-siRNA transfected cells were significantly ***,in order to determine the effects of pyrrolidinediethyldithiocarbamate (PDTC),an NF-κB inhibitor,on the TLR4-induced TLR2 expression in BV-2 cells treated with ischemia/reperfusion,it was found that TLR4 and TLR2 mRNA expressions in PDTC pretreated cells were significantly lower in comparison with normal saline pretreated cells and non-pretreated *** data suggested that TLR2 activation,signaled by TLR4 and regulated by NF-κB,might be directly involved play an important role in ischemia/reperfusion induced brain damage.

关键词： Toll-like receptor 4 Toll-like receptor 2 cerebral ischemia/reperfusion microglia

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2018 Chinese Pediatric Cardiology Society(CPCS) guideline for diagnosis and treatment of syncope in children and adolescents

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science Bulletin 2018年第23期63卷 1558-1564页

作者： Cheng Wang Yaqi Li Ying Liao Hong tian Min Huang Xiangyu Dong Lin Shi jinghui sun Hongfang Jin Junbao Du Jindou An Jie Chen Mingwu Chen Qi Chen sun Chen Yonghong Chen Zhi Chen Adolphus Kai-tung Chau Junbao Du Zhongdong Du Junkai Duan Hongyu Duan Xiangyu Dong Lin Feng Lijun Fu Fangqi Gong Yonghao Gui Ling han Zhenhui han Bing He Zhixu He Xiufen Hu Yimin Hua Guoying Huang Min Huang Ping Huang Yujuan Huang Hongfang Jin Mei Jin Bo Li Fen Li Tao Li Xiaohui Li Xiaoyan Liu Yan Li Haitao Lv Tiewei Lv Zipu Li Luyi Ma Silin Pan Yusheng Pang Hua peng Yuming Qin Jie Shen Lin Shi Kun sun jinghui sun Hong tian Jie tian Cheng Wang Hong Wang Lei Wang Jinju Wang Wendi Wang Yuli Wang Rongzhou Wu tianhe Xia Yanyan Xiao Chunhong Xie Yanlin Xing Zhenyu Xiong Baoyuan Xu Yi Xu Hui Yan Shiwei Yang Qijian Yi Xia Yu Xianyi Yu Yue yuan Hongyan zhang Huili zhang Li zhang qingyou zhang Xi zhang Yanmin zhang Zhiwei zhang Cuifen Zhao Bin Zhou Hua Zhu department of Pediatric Cardiovasoloy Children's Medical CenterThe Second Xiangya HospitalCentral South UniversityChangsha 410071China department of Pediatrics Peking University First HospitalBeijing 100034China department of Pediatric Cardiology Children's Hospital of Fudan UniversityShanghai 201102China department of Pediatric Cardiology Shanghai Children's HospitalShanghai 207102China of Pediatrics the 2nd Hospital of Lanzhou UniversityLanzhou 730020China department of Pediatric Cardiology Capital Institute of PediatricsBezjing 100020China department of Pediatrics Jilin University First HospitalChangchun 130021China Chinese Pediatric Cardiology Society Chinese Pediatric Society(CPCS)Chinese Medical Association(CMA)China Committee on Pediatric Syncope Pediatricians BranchChinese Medical Doctor Association(CMDA)China Pediatric Cardiology Society Beijing Pediatric SocietyBeijing Medical Association(BMA)China Committee on Pediatric Cardiology Chinese College of Cardiovascular PhysiciansChinese Medical Doctor Association(CMDA)China

Syncope belongs to the transient loss of consciousness(TLOC), characterized by a rapid onset, short duration, and spontaneous complete recovery. It is common in children and adolescents, accounting for 1% to 2% of emergency department *** syncope can seriously affect children's physical and mental health, learning ability and quality of life and sometimes cardiac syncope even poses a risk of sudden death. The present guideline for the diagnosis and treatment of syncope in children and adolescents was developed for guiding a better clinical management of pediatric syncope. Based on the globally recent development and the evidence-based data in china, 2018 Chinese Pediatric Cardiology Society(CPCS) guideline for diagnosis and treatment of syncope in children and adolescents was jointly prepared by the Pediatric Cardiology Society, Chinese Pediatric Society, Chinese medical Association(CMA)/Committee on Pediatric Syncope, Pediatricians Branch, Chinese medical Doctor Association(CMDA)/Committee on Pediatric Cardiology, Chinese college of Cardiovascular Physicians, Chinese medical Doctor Association(CMDA)/Pediatric Cardiology Society, Beijing Pediatric Society, Beijing medical Association(BMA). The present guideline includes the underlying diseases of syncope in children and adolescents, the diagnostic procedures, methodology and clinical significance of standing test and headup tilt test, the clinical diagnosis vasovagal syncope, postural orthostatic tachycardia syndrome, orthostatic hypotension and orthostatic hypertension, and the treatment of syncope as well as follow-up.

关键词： CPCS Children Adolescent syncope

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TLR4基因小干扰RNA对缺氧复氧诱导BV-2细胞炎症因子分泌的影响

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中华急诊医学杂志 2009年第3期18卷 270-273页

作者：孙鹏韩继媛张清张景辉田元华中科技大学同济医学院附属协和医院急诊科武汉430622

目的研究Toll样受体4（TLR4）基因小干扰RNA（siRNA）对体外缺氧复氧条件下诱导BV-2细胞分泌TNF-α的抑制作用。方法小鼠小胶质细胞株BV-2置于六孔培养板培养，随机分为N组（正常培养组）、H组（缺氧复氧组）、T组（缺氧复氧＋TLR4-siRN... 详细信息

目的研究Toll样受体4（TLR4）基因小干扰RNA（siRNA）对体外缺氧复氧条件下诱导BV-2细胞分泌TNF-α的抑制作用。方法小鼠小胶质细胞株BV-2置于六孔培养板培养，随机分为N组（正常培养组）、H组（缺氧复氧组）、T组（缺氧复氧＋TLR4-siRNA转染组，转染质粒pEGFP-H1／TLR4-siRNA）、C组（缺氧复氧＋对照siRNA转染组，转染含对照序列的质粒pEGFP-H1／对照-siRNA）和B组（缺氧复氧＋空白质粒转染组，转染pEGFP-H1空质粒）共5组。其中H组、T组、C组和B组细胞缺氧培养3h后复氧培养24h，运用脂质体转染技术介导质粒转染，流式细胞术检测转染后BV-2细胞EGFP的表达率，RT-PCR方法检测各组BV-2细胞TLR4 mRNA和NF-κB p65 mRNA的表达水平，Western blot方法检测各组BV-2细胞TLR4蛋白表达变化，ELISA方法检测各组上清液中TNF-α含量。采用方差分析进行统计分析。结果转染后BV-2细胞EGFP的表达率为（67．58-＋27．16）％；经缺氧复氧处理后，H组、T组、C组和B组的TLR4 mRNA、NF-κB p65 mRNA、TLR4蛋白水平较N组均明显上调（P〈0．01），各组上清液TNF-α含量较N组也明显升高（P〈0．01）：而T组TLR4 mRNA、NF-κB p65 mRNA、TLR4蛋白表达量和上清液TNF-α含量较H组、C组和B组均明显下调（P〈0．01）；C组和B组分别与H组相比，各项检测指标表达均无明显变化（P〉0．05）。结论针对TLR4 mRNA的小干扰RNA可以有效的抑制缺氧复氧诱导的BV-2细胞的炎症反廊。

关键词：小干扰RNA 缺氧复氧 Toll样受体4 小胶质细胞

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