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Advances in oral mesenchymal stem cell-derived extracellular vesicles in health and disease

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Genes & Diseases 2024年第1期11卷 346-357页

作者： Huanyu Luo Anahid Ahmadi Birjandi Feilong Ren Tianmeng sun Paul T.Sharpe hongchen sun Zhengwen An Department of Oral Biology Jilin Provincial Key Laboratory of Tooth Development and Bone RemodelingSchool and Hospital of StomatologyJilin UniversityChangchunJilin 130021China Faculty of Dentistry Oral&Craniofacial SciencesCentre for Craniofacial and Regenerative BiologyKing's College LondonLondonSE19RTUK Department of Oral Pathology Jilin Provincial Key Laboratory of Tooth Development and Bone RemodelingSchool and Hospital of StomatologyJilin UniversityChangchunJilin 130021China

Extracellular vesicles(EVs)are nano-size vesicles secreted naturally by all cells into the extracellular space and have been recognized as important cell-cell mediators in multicel-lular *** contain nucleic acids,proteins,lipids,and other cellular components,regulating many basic biological processes and playing an important role in regenerative med-icine and *** can be traced to their cells of origin and exhibit a similar ***,EVs demonstrate low immunogenicity,good biocompatibility,and fewer side ef-fects,compared to their parent *** stem cells(MsCs)are one of the most important resource cells for EVs,with a great capacity for self-renewal and multipotent differ-entiation,and play an essential role in stem cell *** mechanism of MsC therapy was thought to be attributed to the differentiation of MsCs after targeted migration,as previously ***,emerging evidence shows the previously unknown role of MsC-derived para-crine factors in stem cell *** EVs derived from oral tissue MSCs(OMSC-EVs),show more advantages than those of all other MsCs in tissue repair and regeneration,due to their lower invasiveness and easier accessibility for sample ***,we systematically review the biogenesis and biological characteristics of OMSC-EVs,as well as the role of OMsC-EVs in intercellular ***,we discuss the potential therapeutic roles of OMSC-EVs in oral and systemic *** highlight the current challenges and future directions of OMSC-EVs to focus more attention on clinical *** aim to provide valuable insights for the explorative clinical application of OMSC-EVs.

关键词： Disease Extracellular vesicles Oral tissue MSC Therapeutic application Tissue regeneration

Rational design of hydrogels for immunomodulation

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Regenerative Biomaterials 2022年第1期9卷 979-1000页

作者： Wenhuan Bu Yuanhao Wu Amir M.Ghaemmaghami hongchen sun Alvaro Mata Liaoning Provincial Key Laboratory of Oral Diseases School of StomatologyChina Medical UniversityNo.117Nanjing North StreetHeping DistrictShenyang 110001China Department of Dental Materials School of StomatologyChina Medical UniversityNo.117Nanjing North StreetHeping DistrictShenyang 110001China Department of Center Laboratory School of StomatologyChina Medical UniversityNo.117Nanjing North StreetHeping DistrictShenyang 110001China School of Pharmacy University of NottinghamUniversity ParkNottingham NG72RDUK Biodiscovery Institute University of NottinghamUniversity ParkNottingham NG72RDUK Division of Immunology School of Life SciencesUniversity of NottinghamUniversity ParkNottingham NG72RDUK Terasaki Institute for Biomedical Innovation 1018 Westwood BlvdLos AngelesCA 90024USA Department of Chemical and Environmental Engineering University of NottinghamUniversity ParkNottingham NG72RDUK

The immune system protects organisms against endogenous and exogenous harm and plays a key role in tissue development,repair and *** immunomodulatory biologics exhibit limitations including degradation by enzymes,short half-life and lack of targeting *** or binding these biologics within biomaterials is an effective way to address these *** are promising immunomodulatory materials because of their prominent biocompatibility,tuneability and ***,to take advantage of these opportunities and optimize material performance,it is important to more specifically elucidate,and leverage on,how hydrogels affect and control the immune ***,we summarize how key physical and chemical properties of hydrogels affect the immune *** first provide an overview of underlying steps of the host immune response upon exposure to ***,we discuss recent advances in immunomodulatory strategies where hydrogels play a key role through(i)physical properties including dimensionality,stiffness,porosity and topography;(ii)chemical properties including wettability,electric property and molecular presentation;and(iii)the delivery of bioactive molecules via chemical or physical ***,this review aims to build a conceptual and practical toolkit for the design of immune-instructive hydrogels capable of modulating the host immune response.

关键词： hydrogels immunomodulation physical properties chemical properties

Polycation-functionalized gold nanodots with tunable near-infrared fluorescence for simultaneous gene delivery and cell imaging

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Nano Research 2018年第5期11卷 2392-2404页

作者： Yuanqing sun Dandan Wang Yueqi Zhao Tianxin Zhao hongchen sun Xiangwei Li Chuanxi Wang Bai Yang Quan Lin State Key Laboratory of Supramolecular Structure and Materials College of Chemistry Jilin University Changchun 130012 China Institute of New Energy Technology Ningbo Institute of Industrial Technology Chinese Academy of Sciences Ningbo 315201 China School ofStomatology Jilin University Changchun 130041 China State Key Laboratory of Heavy Oil Processing College of Science China University of Petroleum Beijing 102249 China

Near-infrared （NIR） fluorescent metal nanodots may have significant advantages in biological detection and bioimaging. Herein, we introduce tunable near-infrared fluorescent gold nanodots （AuNDs） protected by branched polyethylenimine （PEI） modified by surface segmental attachment of sulfhydryl groups （PEI-SH）, abbreviated as PEI-SH-AuNDs, for simultaneous gene delivery and cell imaging. The modified PEI endows the resultant PEI-SH-AuNDs with the following excellent advantages. Sulfhydryl groups of PEI-SH anchor to the surface of AuNDs, and such polycations with amine groups give PEI-SH-AuNDs remarkable stability. The cationic polymer PEI-SH with positive charges enables PEI-SH-AuNDs to perform gene delivery, and the gene transfection efficiency can reach 22.8%. Moreover, the fluorescence of PEI-SH-AuNDs is tunable from visible red light （wavelength 609 nm） to NIR light （wavelength 811 run） via an increase in the size of AuNDs. PEI-SH-AuNDs yielded gene transfection efficiency similar to that of commercial PEI, but showed much lower cytotoxicity and much greater red-shift fluorescence. With excellent photoluminescent properties, such multifunctional fluorescent PEI-SH-AuNDs hold promise in applications to bioimaging and as ideal fluorescent probes for tracking gene transfection behavior.

关键词： near-infrared fluorescent material Au nanodot tunable fluorescence gene deliver bioimaging

Resistive Switching Properties and Failure Behaviors of(Pt, Cu)/Amorphous ZrO_(2)/Pt Sandwich Structures

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Journal of Materials Science & Technology 2016年第7期32卷 676-680页

作者： Haifa Zhai Jizhou Kong Jien Yang Jing Xu Qingran Xu hongchen sun Aidong Li Di Wu Henan Province Key Laboratory of Photovoltaic Materials College of Physics and Electronic Engineering Henan Normal University National Laboratory of Solid State Microstructures Department of Materials Science and Engineering College of Engineering and Applied SciencesNanjing University State Key Laboratory of Mechanics and Control of Mechanical Structures Nanjing University of Aeronautics and Astronautics

The effect of Pt and Cu electrodes on the resistive switching properties and failure behaviors of amor- phous ZrO2 films were investigated. Compared with Cu/ZrO2/Pt structures, the Pt/ZrO2/Pt structures exhibit better resistive switching properties such as the higher resistance ratio of OFF/ON states, the longer switch- ing cycles and narrow distribution of OFF state resistance （Roff）. The switching mechanism in the Pt/ZrO2/ Pt structure can be attributed to the formation and rupture of oxygen vacancy filaments; while in the Cu/ZrO2/Pt structure, there exist both oxygen vacancy filaments and Cu filaments. The formation of Cu filaments is related to the redox reaction of Cu electrode under the applied voltage. The inhomoge- neous dispersive injection of Cu ions results in the dispersive Ro~ and significant decrease of operate voltage, Schematic diagrams of the formation of conductive filaments and the failure mechanism in the Cu/ZrO2/ Pt structures are also proposed.

关键词： ZrO2 Thin films Sol-gel method Failure

Preparation and Characterization of CaO/ZnO Core-shell Structured Nanoparticles

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Chemical Research in Chinese Universities 2020年第5期36卷 970-975页

作者： WANG Lili LIU Yanan PENG Xiaoxing sun Yuanqing LIU Xiaohua LIU Huimin LIN Quan sun hongchen YANG Bai LI Xiangwei School of Stomatology Jilin UniversityChangchun 130021P.R.China Hospital Attached to Changchun University of Chinese Medicine Changchun 130041P.R.China State Key Laboratory of Supramol ecular Structure and Materials Jilin UniversityChangchun 130012P.R.China Department of Biomedical Sciences Texas A&M UniversityCollege of DentistryDallasTX 75246USA

Residual bacteria and microleakage in a complicated root canal can often result in reinfection of the periapical *** promote the antibacterial and sealing eftects of a root canal filling,core-shell structured CaO/ZnO nanospheres were synthesized using a precipitation method based on a traditional root canal sealer,zinc oxide-eugenol(ZOE).The obtained CaO/ZnO particles had a size of 80—90 nm and a core-shell *** film thickness,flow rate,pH,and calcium ion release of the core-shell structured CaO/ZnO nanospheres-eugenol paste were *** pH and calcium ion release results showed a slight increase in the prepared nanospheres,with a flow rate of 24.22 mm and a formed film thickness of 30 pm,which are basically consistent with ISO 6876:2001 standards(regarding dental root canal sealing materials).Cytotoxicity tests showed that the cytocompatibility of the CaO/ZnO nanospheres-eugenol paste was much higher than that of the ZOE or iRoot SP groups(P<0.05).A comparison of the sealing ability and antibacterial activity showed that the core-shell structured CaO/ZnO nano spheres-eugenol paste had significantly better effects than the ZOE and iRoot SP sealers(P<0.05).The core-shell structured CaO/ZnO nano sphere paste displayed excellent sealing and antibacterial properties,and it has promising application potential in endodontics.

关键词： CaO ZnO Antibacterial activity Core-shell stnictured naiiosphere Microleakage

Ultra-small nanodots coated with oligopeptides providing highly negative charges to enhance osteogenic differentiation of hBMSCs better than osteogenic induction medium

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Chinese Chemical Letters 2021年第1期32卷 266-270页

作者： Yueqi Zhao Lin Meng Kai Zhang Yuanqing sun Yue Zhao Zhe Yang Yangliu Lin Xinrui Liu hongchen sun Bai Yang Quan Lin State Key Laboratory of Supramolecular Structure and Material College of ChemistryJilin UniversityChangchun 130012China School and Hospital of Stomatology China Medical UniversityShenyang 110002China State Key Laboratory of Heavy Oil Processing College of ScienceChina University of PetroleumBeijing 102249China School and Hospital of Stomatology Jilin UniversityChangchun 130021China Department of Neurosurgical Oncology The First Hospital of Jilin UniversityChangchun 130021China

For bone regenerative engineering,it is a promising method to form skeletal tissues differentiating from human bone morrow mesenchyme stem cells(hBMSCs).However,it is still a critical challenge to efficiently control ostogenesis and clearly reveal the influence *** this end,the fluorescent gold nanodots(Au NDs) with highly negative charges as osteogenic induction reagent are successfully synthesized,which display better than commercial osteogenic induction medium through the investigations of ALP activity(2.5 folds) and cytoskeleton staining(1.5 folds).Two kinds of oligopeptides with different bio-structures(cysteine,Cys and glutathione,GSH) are selected for providing surficial charges on Au *** is revealed that Au-Cys with more negative charges(-51 mV) play better role than Au-GSH(-19 mV) in osteogenic differentiation,when both of them have same size(~2 nm),sphere shape and show similar cell uptake *** explore deeply,osteogenesis related signaling pathways are monitored,revealing that the enhancement of osteogenic differentiation was through autophagy signaling pathway triggered by *** the promotion of highly negative charges in osteogenic diffe rentiation was further proved via sliver nanodots(Ag NDs,Ag-Cys and Ag-GSH) and carbon nanodots(CDs,Cys-CDs and GSH-CDs).This work indicates part of insights during hBMSCs differentiation and provides a novel strategy in osteogenic differentiation process.

关键词： Osteogenic differentiation Negative charges from oligopeptide Sub-3 nm fluorescent Au nanodots Better than osteogenic induction medium Autophagy

Regulation of FN1 degradation by the p62/SQSTM1-dependent autophagy-lysosome pathway in HNSCC

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International Journal of Oral Science 2020年第4期12卷 327-337页

作者： Xinchen Liu Lin Meng Xing Li Daowei Li Qilin Liu Yumeng Chen Xiangwei Li Wenhuan Bu hongchen sun Department of Oral Biology Jilin Provincial Key Laboratory of Tooth Development and Bone RemodelingSchool and Hospital of StomatologyJilin UniversityChangchunChina Department of Endodontics School and Hospital of StomatologyJilin UniversityChangchunChina Department of Pathology School and Hospital of StomatologyJilin UniversityChangchunChina School and Hospital of Stomatology China Medical UniversityShenyangChina

Epithelial–mesenchymal transition(EMT)is involved in both physiological and pathological *** plays an essential role in the invasion,migration and metastasis of *** has been shown to regulate EMT in a variety of cancers but not in head and neck squamous cell carcinoma(HNSCC).Herein,we investigated whether autophagy also regulates EMT in *** of clinical data from three public databases revealed that higher expression of fibronectin-1(FN1)correlated with poorer prognosis and higher tumour pathological grade in *** from SCC-25 cells demonstrated that rapamycin and Earle’s balanced salt solution(EBSS)promoted autophagy,leading to increased FN1 degradation,while 3-methyladenine(3-MA),bafilomycin A1(Baf A1)and chloroquine(CQ)inhibited autophagy,leading to decreased FN1 *** the other hand,autophagic flux was blocked in BECN1 mutant HNSCC Cal-27 cells,and rapamycin did not promote autophagy in Cal-27 cells;also in addition,FN1 degradation was ***,we identified FN1 degradation through the lysosome-dependent degradation pathway using the proteasome inhibitor *** from immunoprecipitation assays also showed that p62/SQSTM1 participated as an autophagy adapter in the autophagy–lysosome pathway of FN1 ***,data from immunoprecipitation assays demonstrated that the interaction between p62 and FN1 was abolished in p62 mutant MCF-7 and A2780 cell *** results indicate that autophagy significantly promotes the degradation of ***,our findings clearly suggest that FN1,as a marker of EMT,has adverse effects on HNSCC and elucidate the autophagy–lysosome degradation mechanism of FN1.

关键词： inhibited invasion FN1

Construction of hollow polydopamine nanoparticle based drug sustainable release system and its application in bone regeneration

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International Journal of Oral Science 2021年第3期13卷 282-291页

作者： Lu Wang Shuwei Liu Chunxia Ren Siyuan Xiang Daowei Li Xinqing Hao Shilei Ni Yixin Chen Kai Zhang hongchen sun Department of Oral Pathology Hospital of StomatologyJilin UniversityChangchunChina Jilin Provincial Key Laboratory of Tooth Development and Bone Remodeling School and Hospital of StomatologyJilin UniversityChangchunChina State Key Laboratory of Supramolecular Structure and Materials College of ChemistryJilin UniversityChangchunChina

Nanomaterial-based drug sustainable release systems have been tentatively applied to bone ***,however,still face disadvantages of high toxicity,low biocompatibility,and low drug-load *** view of the low toxicity and high biocompatibility of polymer nanomaterials and the excellent load capacity of hollow nanomaterials with high specific surface area,we evaluated the hollow polydopamine nanoparticles(HPDA NPs),in order to find an optimal system to effectively deliver the osteogenic drugs to improve treatment of bone *** demonstrated that the HPDA NPs synthesized herein could efficiently load four types of osteogenic drugs and the drugs can effectively release from the HPDA NPs for a relatively longer time in vitro and in vivo with low toxicity and high *** of qRT-PCR,ALP,and alizarin red S staining showed that drugs released from the HPDA NPs could promote osteogenic differentiation and proliferation of rat bone marrow mesenchymal stem cells(rBMSCs)in *** data from micro-CT and H&E staining showed that all four osteogenic drugs released from the HPDA NPs effectively promoted bone regeneration in the defect of tooth extraction fossa in vivo,especially *** results suggest that the HPDA NPs,the biodegradable hollow polymer nanoparticles with high drug load rate and sustainable release ability,have good prospect to treat the bone defect in future clinical practice.

关键词： drugs dopamine release

Research Progress on the Role of Endoplasmic Reticulum Stress in Osteogenic Differentiation

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Journal of Clinical and Nursing Research 2020年第2期4卷 61-64页

作者： Nianqiang Jin hongchen sun School of Stomatology China Medical UniversityShenyang 110000China

The endoplasmic reticulum(ER)is the main site for regulating protein synthesis and *** reticulum stress plays a role in regulating the osteogenic differentiation of stem cells and general *** marrow stromal cells(BMSCs,also known as bone marrow mesenchymal stem cells)are a group of progenitor cells that contain a small number of bone stem cells(SSCs)that rebuild cartilage,bone,stroma,and fat cells that support hematopoiesis and bone ***,due to their self-renewal and differentiation capabilities,they have become an important resource for researching regenerative medicine and tissue engineering treatment *** of osteoblasts to physical and biochemical stimuli facilitates rapid activation of early tissue repair processes in ***,the rational regulation of the induction conditions of osteoblasts has become a hot research *** article reviews the recent advances in the role of endoplasmic reticulum stress in the process of osteoblast differentiation.

关键词： Endoplasmic reticulum stress BMSC Osteoblast Osteoblasts differentiation