Ultra-small nanodots coated with oligopeptides providing highly negative charges to enhance osteogenic differentiation of hBMSCs better than osteogenic induction medium

作     者：Yueqi Zhao Lin Meng Kai Zhang Yuanqing Sun Yue Zhao Zhe Yang Yangliu Lin Xinrui Liu Hongchen Sun Bai Yang Quan Lin Yueqi Zhao;Lin Meng;Kai Zhang;Yuanqing Sun;Yue Zhao;Zhe Yang;Yangliu Lin;Xinrui Liu;Hongchen Sun;Bai Yang;Quan Lin

作者机构：State Key Laboratory of Supramolecular Structure and MaterialCollege of ChemistryJilin UniversityChangchun 130012China School and Hospital of StomatologyChina Medical UniversityShenyang 110002China State Key Laboratory of Heavy Oil ProcessingCollege of ScienceChina University of PetroleumBeijing 102249China School and Hospital of StomatologyJilin UniversityChangchun 130021China Department of Neurosurgical OncologyThe First Hospital of Jilin UniversityChangchun 130021China 

出 版 物：《Chinese Chemical Letters》 (中国化学快报（英文版）)

年 卷 期：2021年第32卷第1期

页      面：266-270页

核心收录：

学科分类：07[理学] 070205[理学-凝聚态物理] 08[工学] 080501[工学-材料物理与化学] 0805[工学-材料科学与工程（可授工学、理学学位）] 080502[工学-材料学] 0702[理学-物理学] 

基　　金：supported by the National Nature Science Foundation of China(Nos.51861145311,22005338) Science Foundation of China University of Petroleum,Beijing(No.2462017YJRC027) Open Project of State Key Laboratory of Superhard Materials(Jilin University 201802) 

主　　题：Osteogenic differentiation Negative charges from oligopeptide Sub-3 nm fluorescent Au nanodots Better than osteogenic induction medium Autophagy 

摘      要：For bone regenerative engineering,it is a promising method to form skeletal tissues differentiating from human bone morrow mesenchyme stem cells(hBMSCs).However,it is still a critical challenge to efficiently control ostogenesis and clearly reveal the influence *** this end,the fluorescent gold nanodots(Au NDs) with highly negative charges as osteogenic induction reagent are successfully synthesized,which display better than commercial osteogenic induction medium through the investigations of ALP activity(2.5 folds) and cytoskeleton staining(1.5 folds).Two kinds of oligopeptides with different bio-structures(cysteine,Cys and glutathione,GSH) are selected for providing surficial charges on Au *** is revealed that Au-Cys with more negative charges(-51 mV) play better role than Au-GSH(-19 mV) in osteogenic differentiation,when both of them have same size(~2 nm),sphere shape and show similar cell uptake *** explore deeply,osteogenesis related signaling pathways are monitored,revealing that the enhancement of osteogenic differentiation was through autophagy signaling pathway triggered by *** the promotion of highly negative charges in osteogenic diffe rentiation was further proved via sliver nanodots(Ag NDs,Ag-Cys and Ag-GSH) and carbon nanodots(CDs,Cys-CDs and GSH-CDs).This work indicates part of insights during hBMSCs differentiation and provides a novel strategy in osteogenic differentiation process.