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Purification and Trypsin Inhibitor Activity of a Sporamin B from Sweet Potato (Ipomoea batatas Lam. 55-2)

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Agricultural Sciences in China 2009年第7期8卷 808-820页

作者： sun Yan-li sun Jun-mao LI Qing-peng Institute of Agro-Food Science ＆ Technology Chinese Academy of Agricultural Sciences/Key Laboratory of Agricultural Product Processing and Quality Control Ministry of Agriculture Beijing 100193 P.R.China Agricultural Information Institute Chinese Academy of Agricultural Sciences Beijing 100081 P.R.China

Sporamin is a soluble protein in sweet potato, and falls into two distinct homology groups, subfamilies A and B. In this research, a sporamin B was purified and its amino acid sequences, trypsin inhibitor activity （Ti activity） were analyzed. This sporamin B was isolated from sweet potato tubers [Ipomoea batatas （L.） Lam cv. 55-2] through extraction of the water-soluble fraction, dialysis, ultrafiltration and ion-exchange chromatography. Homology determined by polyacrylamide gel electrophoresis showed that mainly one bond appeared in gel after being reduced by SDS （sodium dodecyl sulfate）, or by SDS and 2-mercaptoethanol, or in native situation. By comparing the data of the polypeptide mass Matrix-assisted laser desorption/ionization time-of-flight （MALDI-TOF） mass spectrometry with those of the mass of the theoretical amino acid sequences from nCBI protein database, it was revealed that it was Q40091｜Q40091_IPOBA, sweet potato sporamin B - Ipomoea Batatas （sweet potato）（Batate）. The sequence coverage was 70.6%. n-terminal sequence was SETPV （Ser-Glu-Thr-Pro-Val）. There is a linear relationship between trypsin inhibitor activity （Ti activity） and amounts of this sporamin B （3-18 μg mL-1）. The equation of linear regression was y = 2.5809x ＋ 17.049 （r2 = 0.9966）. There was a curvilinear relationship between Ti activity and amounts of this sporamin B （21-150 μg mL-1）. The equation of curvilinear regression is y = 14.417ln（x）＋ 23.26 （r2 = 0.9924）. The concentration of sporamin B with Ti activity after heating at 40°C may induce part denature of this sporamin B, and there was no statistic difference after heating at 40, 50, 60°C for 20 min. Heat treatment at more than 90°C leads to a dramatic decrease of trypsin inhibitor efficiency. The results suggested that Q40091｜Q40091_IPOBA was the major sporamin B in sweet potato tubers [Ipomoea Batatas （L.） Lam cv. 55-2], which had strong Ti activity, and was stable to both thermal and DTT （DL-dithiothreitol） relatively.

关键词： sweet potato sporamin B identification trypsin inhibitor activity

Karyomorphology of three endemic plants(Brassicaceae:Euclidieae and Arabideae)from the Qinghai-Tibet Plateau and its significance

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Plant Diversity 2020年第3期42卷 135-141页

作者： Wenguang sun Haixia Wang Rui Wu Hang sun Zhimin Li CAS Key Laboratory for Plant Diversity and Biogeography of East Asia Kunming Institute of BotanyChinese Academy of SciencesKunming 650201China School of Life Science Engineering Research Center of Sustainable Development and Utilization of Biomass EnergyYunnan Normal UniversityKunming 650500.China University of the Chinese Academy of Sciences Beijing 100049China

In the paper,chromosome number and karyotype of three endemic genera from China are reported for the first *** results show that Anzhengxia yechengnica has a karyotype formula2 n=2 x=14=6 m+8 sm and belongs to Stebbins-3 A;Shangrilaia nana karyotype formula is2 n=2 x=14=10 m+4 sm(2 sat) and belongs to Stebbins-1 A;Baimashania pulvinata karyotype formula is 2 n=2 x=16=12 m(2 sat)+4 sm and belongs to Stebbins-1 *** and Shangrilaia are monospecific genera belonging to tribe Euclidieae and both have a basic chromosome x=***,which belongs to tribe Arabideae,has two species which have a basic chromosome x=*** implications of these cytological data are compared with morphological support and the implications for each tribe are *** also summarize chromosomal number variation and its systematic implications of two tribes from the Qinghai-Tibet Plateau.

关键词： Karyotype Endemic species Anzhengxia Shangrilaia Baimashania Qinghai-Tibet Plateau

Effect of nourishing Yin and purging fire Chinese herbal mixture on delaying light-induced premature puberty in rats

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Journal of Traditional Chinese Medicine 2024年第1期44卷 95-102页

作者： sun Yanyan XUE Yuanyuan sun Wen WAnG Yonghong YU Jian Department of Traditional Chinese Medicine Children's Hospital of Fudan UniversityShanghai 201102China Department of Pediatrics Zibo Central HospitalZibo 255000China

OBJECTIVE:To elucidate the mechanism of the nourishing Yin and purging fire Chinese herbal mixture(nYPF)in delaying light-induced premature puberty in ***:Twenty-one days old female SpragueDawley rats were randomly assigned to normal group(n),long light exposure group(L),nYPF and normal saline group(nS).Rats in the L,nYPF and nS groups were exposed to 16 h:350 lux light/8 h:dark,while rats in the n group were exposed to 12 h:50 lux light/12 h:*** and normal saline was administered to the rats in the nYPF group or nS group,respectively,from day *** rats in every group were sacrificed at 9 *** day 28(P28),on the day when rat's vulva opened in the L group(L-VO),on the day when the first estrous interphase occurred in rats of L group(L-E1),and on the day when the second estrous interphase occurred in rats of L group(L-E2),***:On day 34,all rats in the L group,80%of rats in the nS group,40%of rats in the n group,and 20%of rats in the nYPF group showed complete opening of the *** P28,m RnA level of hypothalamic kisspeptin(Kiss-1)in the L group was significantly higher than that in the n group(Pn the L and nS groups had significantly lower hypothalamic argininephenylalanine-amide(RFamide)-related peptide 3(RFRP-3)m RnA levels than those in the n group(PnA level was significantly higher in the nYPF group than that in the L group(Pn index of the L and nS groups was significantly higher than that of the n group(Pnd estradiol(E2)level of the nYPF group was significantly lower than that of the n and nS groups(PnA level in the L and nS groups was significantly higher than that in the n and nYPF groups(PnA level in the L,nYPF,and nS groups was significantly lower than that in the n group(Pnd nS groups was significantly higher than that of the n group(P<0.01),wher

关键词： light puberty precocious kisspeptins RFRP-3 melatonin nourishing Yin and Purging Fire

Pectin fractions extracted sequentially from Cerasus humilis:Their compositions,structures,functional properties and antioxidant activities

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Food Science and Human Wellness 2023年第2期12卷 564-574页

作者： Shikai Zhang Geoffrey InWaterhouse Tingting Cui Dongxiao sun-Waterhouse Peng Wu College of Food Science and Engineering Shandong Agricultural UniversityTaian 271018China School of Chemical Sciences The University of AucklandPrivate Bag 92019New Zealand Biology Institute Qilu University of Technology(Shandong Academy of Sciences)Jinan 250103China

Three pectin fractions(water-soluble fraction(WSF),chelator-soluble fraction(CSF),and sodium carbonatesoluble fraction(nSF))were obtained from Chinese dwarf cherry(Cerasus humilis)*** of them were branched low methoxylated pectins with an amorphous or partially nanocrystalline nature and eight neutral monosaccharides(arabinose and galactose were most abundant).WSF,CSF and nSF had a degree of methylation(DM)of 35.82%,14.85%and 7.13%,uronic acid(UA)content of 76.02%,83.71%and 69.01%,and total protein content of 2.4%,2.1%and 8.8%,*** molecular weights were 340.31,330.16 and 141.31 kg/mol,respectively(analyzed by gel permeation chromatography(GPC)).WSF,CSF and nSF exhibited good rheological,thermal,emulsifying,emulsion-stabilizing,water-adsorbing,oil-binding,cholesterol-binding and antioxidant *** had the highest emulsifying,emulsion stabilizing,water-/oil-/cholesterol-binding and antioxidant capacities,followed by *** had the highest viscosity(406.77 m Pa·s),flowability,and resistance to heat-induced changes/damage,which may be related to its lowest polydispersity index,DM and UA content and highest protein *** three pectin fractions with desirable characteristics can be used as food additives/ingredients and dietary supplements.

关键词： Cerasus humilis fruits Pectin fractions Structural characteristics Processing-related functional properties

CuSO_4 -CATALYZED SELF-InITIATED RADICAL POLYMERIZATIOn OF 2-(n,n- DIMETHYLAMInO)ETHYL METHACRYLATE AS An InTRInSICALLY REDUCInG InIMER

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Chinese Journal of Polymer Science 2013年第8期31卷 1161-1172页

作者： Yan sun 翟光群 Department of Materials Science and Engineering Changzhou University

Since tertiary amines （Cα-H） can be oxidized by peroxides and transition metal cations in high oxidation states into Ca2＋ radicals to initiate vinylic polymerizations of methacrylates, Cu2＋ and 2-（n,n-dimethylamino）ethyl methacrylate （DMAEMA） form a polymerizable redox initiating pair, in which DMAEMA serves as an intrinsically reducing inimer. CuSOa-catalyzed aqueous self-initiated radical polymerizations of DMAEMA were successfully performed at ambient temperature via a continuous Cu2＋-tertiary amine redox initiation based on catalyst regeneration in the presence of O2. The polymerization kinetics was monitored by gas chromatography and the structure of PDMAEMA was characterized by gel- permeation chromatography, nuclear magnetic resonance spectroscopy, laser light scattering and online intrinsic-viscosity analysis. Both the monomer conversion and the molecular weight of PDMAEMA increase with the reaction while the molecular weight distribution maintains rather broad, as the Cu2＋-DMAEMA redox-initiation leads to linear PDMAEMA chains with terminal methacryloxyl moieties, and the Cu2＋-PDMAEMA redox-initiation results in branched chains. The branched topology forms and develops only for the high-MW components of the PDMAEMA. Our results provide a facile strategy to prepare branched polymers from such commercially available intrinsically reducing inimers using a negligible concentration of regenerative air-stable catalysts.

关键词： 2-(n,n-dimethylamino)ethyl methacrylate (DMAEMA) Cu2+-tertiary amine redox initiation Catalystregeneration Intrinsically reducing inimer Self-initiated radical polymerization.

Regulation of graphdiyne-based nanozymes with enhanced oxidaselike activity by cobalt and nitrogen codoping

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nano Research 2025年第1期18卷 21-29页

作者： Jiali Liu Xiaoli Wang Yongfu Lian Xue Wang Xiuxiu Wang Xingfa Gao Baoyun sun Xihong Guo CAS Key Laboratory for Biomedical Effects of nanomaterial&nanosafety Institute of High Energy PhysicsChinese Academy of ScienceBeijing 100049China Key Laboratory of Functional Inorganic Material Chemistry Ministry of EducationSchool of Chemistry and Materials ScienceHeilongjiang UniversityHarbin 150080China Laboratory of Theoretical and Computational nanoscience National Center for Nanoscience and TechnologyBeijing 100190China University of Chinese Academy of Sciences Beijing 100049China

The development of nanozymes with excellent intrinsic oxidase-like activity and specificity has received increasing ***(GDY)could be a promising choice for designing nanozymes with enhanced oxidase(OXD)-like activity due to its unique structure and ***,Co-n-GDY with high OXD activity but no peroxidase(POD)activity was synthesized by codoping of cobalt(Co)and nitrogen(n)into GDY and compared with other GDY-based nanozymes(including GDY,Co-GDY,and n-GDY).Upon analyzing the doping effect of Co and n on the OXD-like and POD-like activities,we found that the combination of Co and n in GDY played a significant role in enhancing the OXD-like activity,and even reversed the POD-like activity of n-GDY to OXD-like activity of *** electrochemical experiment and the theoretical calculations provided an explanation for the mechanism and showed that the activity was closely linked to the reduction ability of O_(2) or H_(2)O_(2) on the nanozyme substrates,which was determined by the ratedetermining step of the catalytic reaction.

关键词： nanozymes oxidase-like activity graphdiyne heteroatom doping regulation

Machine learning driven rational design of dual atom catalysts on graphene for carbon dioxide electroreduction

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nano Research 2025年第1期18卷 186-194页

作者： Dongxu Jiao Xinyi Li Mingzi sun Lin Liu Jinchang Fan Jingxiang Zhao Bolong Huang Xiaoqiang Cui State Key Laboratory of Automotive Simulation and Control School of Materials Science and Engineeringand Key Laboratory of Automobile Materials of MOEJilin UniversityChangchun 130012China Department of Applied Biology and Chemical Technology The Hong Kong Polytechnic UniversityHung HomKowloonHong KongChina College of Chemistry and Chemical Engineering and Key Laboratory of Photonic and Electronic Bandgap MaterialsMinistry of EducationHarbin Normal UniversityHarbin 150025China

The development of high-performance atomiccatalysts for the carbon dioxide reduction reaction(CO_(2)RR)is atime-consuming process due to the complexity of the reactionmechanism and the uncertainty of the active ***,wehave proposed combining density functional theory(DFT)andmachine learning(ML)to investigate the potential of topologicalgraphene-based dual-atom catalysts(DACs)as CO_(2)*** analyzing the ML results,we identify thenumber of d-orbital electrons in the active site as a key factorinfluencing the CO_(2)RR catalytic ***,wepropose a simple descriptor to measure the CO_(2)RR activity ofthese *** findings provide plausible explanations for thesynergistic interactions between bimetallic atoms in CO_(2)RR andallow us to screen the homogeneous ni-ni pair as the mostpromising dual-atom *** work offers a fast MLapproach based on limited DFT calculations to predict the mostelectroactive and stable DACs on carbon support for CO_(2)RR,facilitating rapid screening of high-performance dual-atomcatalysts.

关键词： CO_(2)reaction reduction dual-atom catalysts density functional theory machine learning

Zwitterionic polymer-coated porous poly(vinyl acetate–divinyl benzene)microsphere: A new support for enhanced performance of immobilized lipase

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Chinese Journal of Chemical Engineering 2020年第1期28卷 242-248页

作者： Yifeng Li Chunyu Zhang Yan sun Department of Biochemical Engineering and Key Laboratory of Systems Bioengineering(Ministry of Education) School of Chemical Engineering and TechnologyTianjin UniversityTianjin 300354China

Enzyme immobilization has attracted great attention for improving the performance of enzymes in industrial *** work was designed to create a new support for Candida rugosa lipase(CRL)immobilization.A porous poly(vinyl acetate–divinyl benzene)microsphere coated by a zwitterionic polymer,poly(maleic anhydride-alt-1-octadecene)and n,n-dimethylethylenediamine derivative,was developed for CRL immobilization via hydrophobic *** catalytic activity,reaction kinetics,stabilities and reusability of the immobilized CRL were *** demonstrated the success of the zwitterionic polymer coating and subsequent CRL immobilization on the porous *** immobilized lipase(p2-MS-CRL)reached27.6 mg·g^-1 dry carrier and displayed a specific activity 1.5 times higher than free *** increase of Vmax and decrease of Kmwere also observed,indicating the improvement of catalytic activity and enzyme-substrate affinity of the immobilized ***,p2-MS-CRL exhibited significantly enhanced thermal stability and pH *** improved performance was considered due to the interfacial activation regulated by the hydrophobic interaction and stabilization effect arisen by the zwitterionic polymer *** study has thus proved the advantages of the zwitterionic polymer-coated porous carrier for lipase immobilization and its potential for further development in various enzyme immobilizations.

关键词： Biocatalysis Lipase immobilization Zwitterionic polymer Hydrophobic binding Interfacial activation Stabilization

nondriven Polymer Translocation Through a nanopore: Scaling for Translocation Time with Chain Length

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Chemical Research in Chinese Universities 2011年第6期27卷 1023-1026页

作者： sun Chia-chung State Key Laboratory of Theoretical and Computational Chemistry Institute of Theoretical Chemistry Jilin University

We investigated the dynamics of the passage for a polymer chain through a nanopore in the absence of any external driving force with Weeks-Chandler-Andersen potential in two-dimensional simulations, in particular, focused our attention on the scaling law of the mean translocation time. We found that the effect of hydrodynamic interactions is the major factor in determining the scaling exponents with increasing pore size. The scaling close to n1＋2v was observed when the hydrodynamic interactions were screened in the cases of small pore sizes, while the scaling close to n3v was obtained when the hydrodynamic interactions were present in the cases of large pore sizes.

关键词： Translocation nondriven Hydrodynamic interaction