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Journal of Integrative Agriculture 2014年第9期13卷 1943-1951页

作者： LIU Huan song xi-jiao NI Yue-qun LU Li-na ZHOU Xue-ping WU Jian-xiang Institute of Biotechnology College of Agriculture & Biotechnology Zhejiang University

Rice ragged stunt virus（RRSV） is a serious rice disease in Asia, causing serious yield losses on rice. The capsid protein（CP） gene of the major outer capsid protein of RRSV was expressed in Escherichia coli BL21（DE3） using the pMAL-C2 X expression vector. The recombinant protein was used as the immunogen to immunize BALB/c mice. A hybridoma cell line 8A12 secreting monoclonal antibody（MAb） against RRSV was obtained by fusing mouse myeloma cells（Sp 2/0） with spleen cells from the immunized BALB/c mice. Western blot analysis showed that the MAb 8A12 can specifically react with RRSV CP. Using the MAb, an antigen-coated-plate enzyme-linked immunosorbent assay（ACP-ELISA）, a dot enzyme-linked immunosorbent assay（dot-ELISA）, and immunocapture-RT-PCR（IC-RT-PCR） assay were developed to detect RRSV. The established ACP-ELISA, dot-blot ELISA and IC-RT-PCR methods could detect RRSV in infected rice tissue crude extracts with dilutions of 1：40 960, 1：1 280 and 1：655 360（w/v, g mL-1）, respectively. The ACP-ELISA and dot-blot ELISA methods could detect RRSV in infected insect vector crude extracts with dilutions of 1：12 800 and 1：1 600（an individual planthopper μL-1）, respectively. The field survey revealed that Rice ragged stunt disease occurs on rice in Hainan, Yunnan, Guangxi, Sichuan, Guizhou, Fujian, Hunan, Jiangxi and Zhejiang in China.

关键词： Rice ragged stunt virus rice brown planthopper monoclonal antibody antigen-coated-plate enzyme-linked immunosorbent assay(ACP-ELISA) dot-blot ELISA immunocapture RT-PCR

FUBP3 mediates the amyloid-β-induced neuronal NLRP3 expression

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Neural Regeneration Research 2025年第7期20卷 2068-2083页

作者： Jing Yao Yuan Li xi Liu Wenping Liang Yu Li Liyong Wu Zhe Wang Weihong song The National Clinical Research Center for Geriatric Disease Xuanwu HospitalCapital Medical UniversityBeijingChina Department of Neurology The First Affiliated Hospital of Zhengzhou UniversityZhengzhouHenan ProvinceChina Department of Neurosurgery Xuanwu HospitalCapital Medical UniversityBeijingChina Department of Pediatrics The Second Affiliated Hospital and Yuying Children’s HospitalKey Laboratory of Alzheimer’s Disease of Zhejiang ProvinceInstitute of AgingWenzhou Medical UniversityWenzhouZhejiang ProvinceChina Oujiang Laboratory(Zhejiang Laboratory for Regenerative Medicine Visionand Brain Health)WenzhouZhejiang ProvinceChina

Alzheimer's disease is characterized by deposition of amyloid-β,which forms extracellular neuritic plaques,and accumulation of hyperphosphorylated tau,which aggregates to form intraneuronal neurofibrillary tangles,in the *** NLRP3 inflammasome may play a role in the transition from amyloid-βdeposition to tau phosphorylation and *** NLRP3 is primarily found in brain microglia,and tau is predominantly located in neurons,it has been suggested that NLRP3 expressed by microglia indirectly triggers tau phosphorylation by upregulating the expression of pro-inflammatory ***,we found that neurons also express NLRP3 in vitro and in vivo,and that neuronal NLRP3 regulates tau *** biochemical methods,we mapped the minimal NLRP3 promoter and identified FUBP3 as a transcription factor regulating NLRP3 expression in *** primary neurons and the neuroblastoma cell line Neuro2A,FUBP3 is required for endogenous NLRP3 expression and tau phosphorylation only when amyloid-βis *** the brains of aged wild-type mice and a mouse model of Alzheimer's disease,FUBP3 expression was markedly increased in cortical *** analysis suggested that FUBP3 plays a role in neuron-mediated immune *** also found that FUBP3 trimmed the 5′end of DNA fragments that it bound,implying that FUBP3 functions in stress-induced *** findings suggest that neuronal NLRP3 may be more directly involved in the amyloid-β-to–phospho-tau transition than microglial NLRP3,and that amyloid-βfundamentally alters the regulatory mechanism of NLRP3 expression in *** that FUBP3 was only expressed at low levels in young wild-type mice and was strongly upregulated in the brains of aged mice and Alzheimer's disease mice,FUBP3 could be a safe therapeutic target for preventing Alzheimer's disease progression.

关键词： 5′end trimming Alzheimer's disease amyloid-beta amyloid-β-dependent transcription FUBP3 inflammasome inflammation neuron NLRP3 tau transcription factor

交配后扶桑绵粉蚧雌成虫卵巢结构及其他相关结构的变化(英文)

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环境昆虫学报 2016年第4期38卷 715-722页

作者：黄芳吕要斌赵春玲宋西娇断江省农业科学院植物保护与微生物研究所杭州310029 湖州出入境检验检疫局浙江湖州313000 浙江省农业科学院公共实验室杭州310029

利用显微光镜和超微电镜技术观察明确了扶桑绵粉蚧雌成虫卵巢及相关结构的变化,结果表明该虫的内生殖系统含1对卵巢、输卵管、储精囊和1对附腺。每个卵巢有数以百计的端滋式卵巢小管。卵巢小管缺末端纤丝,包含滋养部分和卵黄部分。储精... 详细信息

利用显微光镜和超微电镜技术观察明确了扶桑绵粉蚧雌成虫卵巢及相关结构的变化,结果表明该虫的内生殖系统含1对卵巢、输卵管、储精囊和1对附腺。每个卵巢有数以百计的端滋式卵巢小管。卵巢小管缺末端纤丝,包含滋养部分和卵黄部分。储精囊表面被丰富的肌原纤维包裹,未经交配的雌虫体内储精囊在显微镜下呈透明状圆形,电镜观察囊内只含液态物质;交配后,储精囊不再维持规则的球状,囊内出现精细胞等物质;精细胞呈典型的"9+2"结构。在初孵化的雌性成虫体内,卵巢小管内的滋养细胞部分中间为营养核,以此联接滋养细胞和卵细胞。卵黄部分包含1个卵细胞。雌性成虫只有通过交配,卵巢内的胚胎才可得以顺利发育;若未经交配,卵巢内的卵细胞将出现发达的内质网结构,标志着细胞将降解而被母体重吸收。

关键词：扶桑绵粉蚧雌成虫生殖系统结构

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本质与价值:数字时代广告本体的再认识

武汉大学学报（哲学社会科学版） 2025年第1期78卷 125-132页

作者：姚曦任文姣武汉大学新闻与传播学院湖北武汉430072 武汉大学媒体发展研究中心

用传播的方式促进从生产到消费转换的行为皆为存在意义上的广告,广告的本体是本质与价值的统一。本体的坚守要建立开放的认识观,在不变的广告本质中解释变化着的广告形态,而非用未触及本体的不确定认识窄化新的广告事实。彰显广告价值... 详细信息

用传播的方式促进从生产到消费转换的行为皆为存在意义上的广告,广告的本体是本质与价值的统一。本体的坚守要建立开放的认识观,在不变的广告本质中解释变化着的广告形态,而非用未触及本体的不确定认识窄化新的广告事实。彰显广告价值需深耕数字广告实践,用创意提升广告的社会吸引力,建构多主体参与的沟通网络。彰显广告价值还需要在理论层面,以本体观为支点,立足于本土广告实践,重新组织数字广告研究范畴,使用大数据技术与混合研究法探究新问题,为广告学知识体系的建构提供框架与方向。

关键词：广告广告本质广告价值广告本体

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Modular assembly of complex C-alkyl glycoside enabled via cooperative NHC and photoredox catalysis

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Science China Chemistry 2025年

作者： Rui-Qiang jiao Ming Li xi Chen Mei-Ze Xu Tian Ding xiao-Ping Gong Zhuo-Mei Li Xue-Yuan Liu Yong-Min Liang State Key Laboratory of Applied Organic Chemistry Lanzhou University College of Chemistry and Chemical Engineering Northwest Normal University

C-Alkyl glycosides are found in natural products and are indispensable in drug development programs,owing to their diverse range of medicinal ***-component reactions involving glycosyl radical addition are well documented and proved efficient for the C-alkyl glycosides ***,multicomponent reactions for the rapid and controllable construction of structurally diversified C-alkyl glycosides in a single step remains a significant challenge due to the issues associated with balancing reactivity,compatibility,and ***,we report an NHC and photoredox co-catalyzed three-component method to access complex and valuable C-alkyl *** protocol exhibits broad substrate scope and excellent chemoand ***,nine types of sugars are compatible with this *** addition,the generality of this catalytic strategy was further highlighted by the potential for scalable production,the feasibility of further modifications of the products and its successful application in the late-stage functionalization of pharmaceutical skeletons.

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BBTV单克隆抗体的制备及其检测应用

农业生物技术学报 2016年第2期24卷 288-294页

作者：宋西娇刘欢周雪平洪健吴舒艾吴建祥浙江大学生物技术研究所/国家水稻生物学重点实验室

香蕉束顶病毒(Banana bunchy top virus,BBTV)是危害香蕉(Musa paradisiaca)生产的重要病毒。本研究旨在以制备的抗BBTV特异性单克隆抗体(monoclonal antibody,MAb)为核心建立检测BBTV的血清学方法,从而为该病毒的诊断和科学防控提供技... 详细信息

香蕉束顶病毒(Banana bunchy top virus,BBTV)是危害香蕉(Musa paradisiaca)生产的重要病毒。本研究旨在以制备的抗BBTV特异性单克隆抗体(monoclonal antibody,MAb)为核心建立检测BBTV的血清学方法,从而为该病毒的诊断和科学防控提供技术支撑。通过改进提纯方法获得了提纯的BBTV,以BBTV病毒粒子为抗原免疫BALB/c小鼠(Mus musculus),经细胞筛选和克隆,获得1株分泌抗BBTV单克隆抗体的杂交瘤细胞22E3,并制备其单抗腹水。用间接酶联免疫吸附试验(indirect-enzyme-linked immunosorbent assay,in-ELISA)方法测定制备的单抗腹水效价达到10-7,抗体类型及亚类为IgG1,κ轻链。Western blot检测表明,该单抗与BBTV外壳蛋白亚基有特异性反应。利用制备的单抗建立了检测BBTV的斑点酶联免疫吸附试验(dot-ELISA)。该方法检测感染BBTV香蕉植物组织粗提液呈特异性阳性反应,而和健康香蕉组织呈阴性反应,说明建立的dot-ELISA方法能特异性地检测香蕉植物组织中的BBTV。灵敏度分析表明,以22E3单抗为核心建立的dot-ELISA方法检测香蕉病组织的灵敏度达1∶640(W/V,g/m L)。田间样品检测结果表明,建立的dot-ELISA方法能准确、可靠地用于香蕉中BBTV病毒的检测。BBTV单克隆抗体的制备及其dot-ELISA血清学检测方法的建立为我国香蕉上BBTV的诊断、无毒苗的生产及科学防控提供了物质和技术支撑。

关键词：香蕉束顶病毒(BBTV) 单克隆抗体 Dot-ELISA

免疫胶体金标记法研究筛管特异质体中微丝来源的细胞骨架成分

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电子显微学报 2018年第4期37卷 376-380页

作者：宋西娇廖珍凤谢礼张恒木洪健浙江省农业科学院公共实验室浙江杭州310021 浙江大学分析测试中心浙江杭州310058 浙江省农业科学院病毒与生物技术研究所浙江杭州310021

叶绿体等细胞器在细胞内的位置维持和运动受细胞骨架的调控。筛管特异质体是筛管细胞内的特征性细胞器,其发生与叶绿体同源。研究利用免疫胶体金标记法结合商品化的微丝蛋白抗体,发现微丝蛋白actin和myosin除了能被标记到叶绿体上,还能... 详细信息

叶绿体等细胞器在细胞内的位置维持和运动受细胞骨架的调控。筛管特异质体是筛管细胞内的特征性细胞器,其发生与叶绿体同源。研究利用免疫胶体金标记法结合商品化的微丝蛋白抗体,发现微丝蛋白actin和myosin除了能被标记到叶绿体上,还能被标记到筛管特异质体上。该结果暗示了微丝蛋白可能也参与了筛管特异质体在筛管细胞中的位置维持和运动,为细胞器运动的研究提供了新线索。

关键词：筛管特异质体微丝蛋白成分免疫胶体金标记法

柑橘碎叶病毒(CTLV)单克隆抗体的制备及其检测应用

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农业生物技术学报 2017年第1期25卷 165-172页

作者：宋西娇段硕洪健周常勇周雪平吴建祥浙江大学生物技术研究所/国家水稻生物学重点实验室西南大学中国农业科学院柑橘研究所

柑橘碎叶病毒(Citrus tatter leaf virus,CTLV)是严重危害世界柑橘(Citrus reticulata)产业的重要病毒,也是柑橘脱毒苗生产上的重要检疫对象。为了建立以抗CTLV特异性单克隆抗体(monoclonal antibody,MAb)为核心的检测CTLV的血清学方法... 详细信息

柑橘碎叶病毒(Citrus tatter leaf virus,CTLV)是严重危害世界柑橘(Citrus reticulata)产业的重要病毒,也是柑橘脱毒苗生产上的重要检疫对象。为了建立以抗CTLV特异性单克隆抗体(monoclonal antibody,MAb)为核心的检测CTLV的血清学方法。本研究以原核表达的CTLV外壳蛋白为抗原免疫BALB/c小鼠(Mus musculus),经细胞融合、筛选和克隆获得3株(6C5,14E11和15F8)分泌抗CTLV单克隆抗体的杂交瘤细胞。分别制备3株杂交瘤细胞的单抗腹水,经鉴定单抗腹水的抗体类型及亚类均为Ig G1、κ链,单抗腹水的间接酶联免疫吸附实验(enzyme-linked immunosorbent assay,ELISA)效价达到10-6以上。三抗体夹心酶联免疫吸附实验(triple antibody sandwich ELISA,TAS-ELISA)和Western blot分析单抗特异性表明,3株单抗均与感染CTLV柑橘病叶有特异性免疫反应,而不与柑橘衰退病毒(Citrus tristeza virus,CTV)、苹果茎沟病毒(Apple stem grooving virus,ASGV)及健康柑橘树叶反应。利用制备的单抗为核心建立能特异性地检测柑橘病叶中CTLV的斑点酶联免疫吸附实验(dot-ELISA)。灵敏度分析表明,以3株单抗为核心建立的dot-ELISA方法检测病叶组织的灵敏度达1∶640(W/V,g/m L)。田间样品检测结果表明,建立的dot-ELISA方法能准确、可靠和灵敏地用于柑橘树中CTLV病毒的检测。本研究为我国柑橘树中CTLV的诊断、无毒苗的生产及科学防控提供了技术支撑。

关键词：柑橘碎叶病毒(CTLV) 单克隆抗体斑点酶联免疫吸附实验(dot-ELISA)

Imperative for long-term management and surveillance in Kawasaki disease

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World Journal of Clinical Cases 2025年第4期13卷 61-63页

作者： Yan Pan Fu-Yong jiao Department of Pediatrics The First Affiliated Hospital of Yangtze UniversityJingzhou 434000Hubei ProvinceChina Shaanxi Kawasaki Disease Diagnosis and Treatment Center Children’s HospitalShaanxi Provincial People’s Hospital of Xi’an Jiaotong UniversityXi’an 710000Shaanxi ProvinceChina

Kawasaki disease(KD)is a significant pediatric vasculitis known for its potential to cause severe coronary artery *** the effectiveness of initial treatments,such as intravenous immunoglobulin,KD patients can experience long-term cardiovascular issues,as evidenced by a recent case report of an adult who suffered a ST-segment elevation myocardial infarction due to previous KD in the World Journal of Clinical *** editorial emphasizes the critical need for long-term management and regular surveillance to prevent such *** drawing on recent research and case studies,we advocate for a structured approach to follow-up care that includes routine cardiac evaluations and preventive measures.

关键词： Kawasaki disease Long-term management Coronary artery aneurysm Surveillance Preventive care

Brain Functional Networks with Dynamic Hypergraph Manifold Regularization for Classification of End-Stage Renal Disease Associated with Mild Cognitive Impairment

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Computer Modeling in Engineering & Sciences 2023年第6期135卷 2243-2266页

作者： Zhengtao xi Chaofan song Jiahui Zheng Haifeng Shi Zhuqing jiao School of Microelectronics and Control Engineering Changzhou UniversityChangzhou213164China School of Computer Science and Artificial Intelligence Changzhou UniversityChangzhou213164China Department of Radiology Changzhou Second People’s Hospital Affiliated to Nanjing Medical UniversityChangzhou213003China

The structure and function of brain networks have been altered in patients with end-stage renal disease(ESRD).Manifold regularization(MR)only considers the pairing relationship between two brain regions and cannot represent functional interactions or higher-order relationships between multiple brain *** solve this issue,we developed a method to construct a dynamic brain functional network(DBFN)based on dynamic hypergraph MR(DHMR)and applied it to the classification of ESRD associated with mild cognitive impairment(ESRDaMCI).The construction of DBFN with Pearson’s correlation(PC)was transformed into an optimization *** convolution and hyperedge convolution superposition were adopted to dynamically modify the hypergraph structure,and then got the dynamic hypergraph to form the manifold regular terms of the dynamic *** DHMR and L_(1) norm regularization were introduced into the PC-based optimization model to obtain the final DHMR-based DBFN(DDBFN).Experiment results demonstrated the validity of the DDBFN method by comparing the classification results with several related brain functional network construction *** work not only improves better classification performance but also reveals the discriminative regions of ESRDaMCI,providing a reference for clinical research and auxiliary diagnosis of concomitant cognitive impairments.

关键词： End-stage renal disease mild cognitive impairment brain functional network dynamic hypergraph manifold regularization classification