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Polymorphism attribution of cSNPs in cancer-related genes located in loss regions with a high frequency of HCC between HBV and health groups

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Frontiers in Biology 2007年第3期2卷 272-275页

作者： WANG Juan NI Hong CHEN Li CHEN Chengbin song wenqin College of Life Sciences Nankai UniversityTianjin 300071China

Cancer-related genes harbored in the loss regions containing a high frequency of hepatocellular carcinoma(HCC)were selected.Related information was gathered and the coding single nucleotide polymorphism(cSNP)sequenc-es were obtained from the single nucleotide polymorphism(SNP)database.The appropriate primers and oligonucleotide probes were then designed in accordance with the SNP sites,and subsequently,the gene chips for detecting SNPs were constructed.Genomic DNA was extracted from blood sam-ples of healthy controls and from patients with HBV infec-tion.The sequences,including the SNPs,were amplified via polymerase chain reaction(PCR)and labeled using digoxi-genin deoxyuridine tri-phosphate(Dig-dUTP).The labeled products were then hybridized with the SNP chips.Results confirmed that the differences in allele frequencies of three SNPs EGFL3(rs947345),Caspase9(rs2308950),and E2F2(rs3218171)were distinct between HBV-infected patients and controls,suggesting that these SNPs ocuring in high frequency in HBV-infected individuals may be associated with susceptibility to HCC.

关键词： tumor-associated genes Hepatocellular B virus(HBV) single nucleotide polymorphism(SNP) susceptibility

Targeted Mutagenesis of NAC Transcription Factor Gene,OsN AC041,Leading to Salt Sensitivity in Rice

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Rice science 2019年第2期26卷 98-108,I0007-I0015页

作者： WANG Bo ZHONG Zhaohui ZHANG Huanhuan WANG Xia LIU Binglin YANG Lijia HAN Xiangyan YU Deshui ZHENG Xuelian WANG Chunguo song wenqin CHEN Chengbin ZHANG Yong College of Life Sciences Nankai University Department of Biotechnology School of Life Sciences and Technology Center for Informational Biology University of Electronic Science and Technology of China

Salinity is a major abiotic stress factor that seriously affects plant growth. Many genes are involved in the response to salt stress with various metabolism pathways. A number of plant transcription factor family genes have been found to be involved in the salt stress response, and NAM, ATAF and CUC(NAC) transcription factors are thought to act as active regulators during abiotic stress, especially salt stress. In this study, we detected a rice NAC transcription factor coding gene, OsNAC041, and confirmed that it influenced the germination of seeds under salt stress and salt tolerance of plants. OsNAC041 was primarily expressed in the leaves and located in the nucleus. Furthermore, the CRISPR/Cas9 method was used to obtain a targeted osnac041 mutant, of which the plant height was higher than that of the wild-type, showing increased salt sensitivity. Moreover, RNA-seq analysis revealed a number of differentially expressed genes(DEGs) involved in several important signaling pathways in the osnac041 mutant. Subsequently, Kyoto Encyclopedia of Genes and Genomes annotation also revealed differential expression of DEGs associated with mitogen-activated protein kinase signaling, peroxisome, eukaryotictype ABC transporters, photosynthesis and plant hormones, which are involved in stress-related signaling pathways. Overall, our study suggested that OsNAC041 was involved in the salt stress response in rice. These findings not only provide empirical evidence of OsNAC041 function, but also provide new insight into its potential application in rice resistance breeding.

关键词： rice NAC transcription factor CRISPR Cas9 RNA-seq salinity

基于改进CART算法的退役动力电池等级筛选方法

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燕山大学学报 2024年第1期48卷 48-53,76页

作者：刘永成刘杰文杨茜宋汶秦郭永吉王兴贵国网甘肃省电力公司经济技术研究院甘肃兰州730050 兰州理工大学电气工程与信息工程学院甘肃兰州730050

针对退役动力电池存在一致性差、等级筛选效率低的难题,提出了一种基于改进CART算法的退役动力电池等级筛选方法。首先,分析了传统CART算法的基本原理,为克服算法计算量大的缺陷,将Fayyad边界点判定定理与CART算法相结合,通过选取属性... 详细信息

针对退役动力电池存在一致性差、等级筛选效率低的难题,提出了一种基于改进CART算法的退役动力电池等级筛选方法。首先,分析了传统CART算法的基本原理,为克服算法计算量大的缺陷,将Fayyad边界点判定定理与CART算法相结合,通过选取属性最优阈值点来减少计算量,提高分类效率;其次,基于代价复杂度后剪枝算法,采用交叉验证法对算法进行进一步优化;最后,将改进CART算法用于退役动力电池筛选分类,实验结果表明改进CART算法在保持较高准确率的情况下,可以有效提高退役动力电池的等级筛选效率。

关键词：退役动力电池等级筛选改进CART算法最优阈值点

基于深度强化学习算法的储能系统盈利策略研究

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电力需求侧管理 2024年第2期26卷 20-26页

作者：杨国山董鹏旭姚苏航王永利宋汶秦周东国网甘肃省电力公司经济技术研究院兰州730050 华北电力大学经济与管理学院北京102206

在高比例新能源接入下,配置储能可以辅助电力系统削峰填谷,平抑波动。然而目前储能系统成本较高,需要政府进行支持。为此,提出了一种储能盈利策略,以在电网、储能运营商和用户组成的电力市场中实现运营利润最大化。结合智能算法提出了... 详细信息

在高比例新能源接入下,配置储能可以辅助电力系统削峰填谷,平抑波动。然而目前储能系统成本较高,需要政府进行支持。为此,提出了一种储能盈利策略,以在电网、储能运营商和用户组成的电力市场中实现运营利润最大化。结合智能算法提出了一种考虑激励的盈利策略,为每个峰值时段的储能系统运营商提供不同权重的奖励分配。该算法一方面基于最小二乘支持向量机的深度学习,来建立价格和负荷预测模型;另一方面基于深度强化学习,考虑电网的峰值状态、用户负荷需求和储能系统运营商利润,确定最优充放电策略。最后通过案例分析,验证该策略可以显著提高储能系统运营商利润并减轻电网压力。

关键词：储能系统盈利策略支持向量机深度强化学习算法

Analysis of genotype polymorphism of tumor-related genes harbored in chromosome arm 1p and 8p in hepatocellular carcinoma patients by cSNP chip

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Frontiers in Biology 2009年第1期4卷 82-88页

作者： Juan WANG wenqin song College of Life Sciences Shenzhen UniversityShenzhen 518060China College of Life Sciences Nankai UniversityTianjin 300710China

The majority of single nucleotide polymorphisms(SNPs)found in the coding region(cSNPs)are single base substitutions that may or may not lead to amino acid substitutions,most of which are related to diseases.Some cSNPs may prove useful for their potential links to functional cSNPs via linkage disequilibrium mapping.We have selected 48 cSNPs located in the coding regions of 25 genes to construct the cSNP chip.These genes are harbored in the high frequency loss regions of the chromosome 1p and 8p and related with apoptosis,cell cycles,signal transduction,oncogene,tumor suppressor genes and so on.All of the cSNPs can lead to amino acid substitutions except TP73(rs1801174).The PCR products amplified from 31 hepatocellular carcinoma(HCC)specimens were labeled with Dig-dUTP and then hybridized with the cSNP chips.The results showed that there was no hybridization signal when there was more than one site of mutation in the amplification sequence,indicating that the cSNP chip had a high sensitivity.The statistic data of the SNP(MT,homozygous and HT,heterozygous)in the HCC patients with different phenotypes(HBV+/-,differentiation stage,family history positive or negative,tumor size)indicated that the number of MT was distinctly different between patients with positive HBV and negative HBV.The MT and HT numbers of all the 48 cSNPs were significantly different between low differentiation and high differentiation HCC patients.The numbers of MT and HT were not different between positived and negative family history groups and between tumor size>3 cm and≤3 cm groups.The study results provided useful information for understanding the molecular mechanisms of HCC development.

关键词： polymorphism hepatocellular carcinoma single nucleotide polymorphisms(SNPs)in coding region(cSNPs) tumor-related genes 1p and 8p

Homologous analysis on centromeric region sequences of A and B chromosomes from rye by FISH

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Chinese Science Bulletin 1999年第8期44卷 704-707页

作者： Rongxin Zhang Chengbin Chen wenqin song Xiulan Li Ruiyang Chen Nankai Univ Dept Biol Tianjin 300071 Peoples R China

Four centromeric segments from B chromosomes (Bs) of rye have been microdis-sected and amplified by linker adapter PCR (LA-PCR). The PCR products ranged from 100 to 2 000 bp. Fluorescence in situ hybridization (FISH) experiment has been carried out using PCR products as the probe, which was labeled by DIG-11-dUTP. The result confirms that these PCR products from Bs centromeric region are homologous with that of A chromosomes (As) in rye. It also proves that Bs are originated from As.

关键词： B chromosome of rye centromeric region microdissection homology FISH.

Combination losartan with hyaluronic acid modified diethyldithiocarbamate loaded hollow copper sulfide nanoparticles for the treatment of breast cancer and metastasis

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Chinese Chemical Letters 2024年第3期35卷 406-414页

作者： Haili Hu Weiwei Zhang Lei Lei Fan Tong Huilin Zhang Yiwei Zhang wenqin Yang Yilu Tang Ruyi Lin Xue Xia Jiamei Li Shiyong song Huile Gao Key Laboratory of Drug Targeting and Drug Delivery Systems West China School of PharmacySichuan UniversityChengdu 610041China School of Pharmacy Henan UniversityKaifeng 475004China Department of Public Health Chengdu Medical CollegeChengdu 610500China

The application of photothermal therapy(PTT)is greatly limited by the low accumulation of photothermal agents,uneven photothermal distribution,and heat endurance of cancer cells.Worse still,despite PTT enhances immunogenicity,the anti-tumor immune efficacy is still unsatisfactory due to the inefficient immunogenic cell death(ICD)induction and poor infiltration of immune cells.To solve the above problems of PTT,we developed hyaluronic acid(HA)modified hollow copper sulfide nanoparticles encapsulating diethyldithiocarbamate(DDTC)to construct a breast tumor targeting and near infrared(NIR)photo-responsive drug delivery system(D-HCuS@HA),which further combined with losartan to improve the accumulation and penetration in the tumor site.Upon irradiation,D-HCuS@HA realized enhanced PTT and released cytotoxic Cu(DDTC)_(2)to eliminate heat endurance tumor cells,thereby enhancing antitumor effect and inducing effective ICD.Moreover,the combination with losartan could remodel the tumor microenvironment,allowing more T cells to infiltrate into the tumor,and significantly inhibiting the occurrence and development of metastatic tumors.In vitro/vivo results revealed the great potential of D-HCuS@HA combined with losartan,which provides a new paradigm for anti-tumor and anti-metastases.

关键词： Targeted drug delivery system Hollow copper sulfide nanoparticles Photothermal therapy Hyaluronic acid Breast cancer Anti-metastasis

Biochemical analysis of argyrophilic nonhistone proteins in chromosomes of plant Triticum aestivum

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Chinese Science Bulletin 1997年第17期42卷 1476-1481页

作者： PENG Yongkang, YU Jianchun, JIN Shaobo, ZHAO Jian, song wenqin and CHEN RuiyangDepartment of Biology, Nankai University, Tianjin 300071, China Department of Biology Nankai University Tianjin China

IN the late 1970s, biochemical analysis and electron microscopy of isolated, histone-depleted mitotic chromosomes demonstrated the existence of a central scaffold consisting mainly of nonhistone proteins (NHPs) in metaphase chromosomes. Subsequently, by using silver impregnation techniques and light microscopy, Howell et al. showed the presence of an axial chromatid core in relatively intact chromosomes of mammals; this core is believed to be

关键词： plant chromosome nonhistone chromosomal protein argyrophilic protein mitotic synchronization Triticumaestivum.

The learning curve for transurethral enucleation with bipolar energy for benign prostate hyperplasia:a single-surgeon experience of 494 patients

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Asian Journal of Andrology 2024年第3期26卷 288-294页

作者： Byeongdo song Sang Hun song Seong Jin Jeong Department of Urology Seoul National University Bundang HospitalSeongnam 13620Korea Department of Urology Seoul National University College of MedicineSeoul 03080Korea

This study was performed to investigate the learning curve of transurethral enucleation with bipolar energy(TUEB)for benignprostatic hyperplasia.The study involved 494 consecutive patients who underwent TUEB for benign prostatic hyperplasia fromAugust 2018 to March 2022 by one surgeon(SJJ,Seoul National University Bundang Hospital,Seongnam,Korea).The patientswere followed up at 1 week,1 month,3 months,and 6 months postoperatively.To evaluate the learning curve of TUEB,perioperativeparameters including the enucleation ratio(enucleated tissue weight/transitional zone volume),TUEB efficiency(enucleatedtissue weight/operation time),and enucleation efficiency(enucleated tissue weight/enucleation time)were analyzed.Functionaloutcomes and postoperative complications were also assessed,including the International Prostate Symptom Score(IPSS),IPSSquality-of-life(QoL)score,and uroflowmetry outcomes.The patients’median age was 72(interquartile range[IQR]:66–78)years,and the estimated prostate volume and transitional zone volume were 63.0(IQR:46.0–90.6)ml and 37.1(IQR:24.0–60.0)ml,respectively.The enucleation ratio,TUEB efficiency,and enucleation efficiency were 0.60(IQR:0.46–0.54)g ml−1,0.33(IQR:0.22–0.46)g min−1,and 0.50(IQR:0.35–0.72)g min−1,respectively,plateauing after 70 cases.The functional outcomes,including total IPSS,IPSS QoL score,and uroflowmetry outcomes,significantly improved at 6 months after TUEB(all P<0.05),but without significant differences over the learning curve.Sixty-five(13.2%)patients developed complications after TUEB,21.5%of whom experienced major complications(Clavien–Dindo grade≥3).The rate of major complications declined as the number ofTUEB cases increased(P=0.013).Our results suggest that the efficiency of TUEB stabilized within 70 procedures.

关键词： benign prostate hyperplasia learning curve perioperative efficiency prostate transurethral enucleation with bipolar energy