Analysis of genotype polymorphism of tumor-related genes harbored in chromosome arm 1p and 8p in hepatocellular carcinoma patients by cSNP chip

作     者：Juan WANG Wenqin SONG 

作者机构：College of Life SciencesShenzhen UniversityShenzhen 518060China College of Life SciencesNankai UniversityTianjin 300710China 

出 版 物：《Frontiers in Biology》 (生物学前沿（英文版）)

年 卷 期：2009年第4卷第1期

页      面：82-88页

核心收录：

学科分类：0710[理学-生物学] 0831[工学-生物医学工程（可授工学、理学、医学学位）] 0830[工学-环境科学与工程（可授工学、理学、农学学位）] 1002[医学-临床医学] 1001[医学-基础医学(可授医学、理学学位)] 100214[医学-肿瘤学] 0836[工学-生物工程] 0713[理学-生态学] 10[医学] 

主　　题：polymorphism hepatocellular carcinoma single nucleotide polymorphisms(SNPs)in coding region(cSNPs) tumor-related genes 1p and 8p 

摘      要：The majority of single nucleotide polymorphisms(SNPs)found in the coding region(cSNPs)are single base substitutions that may or may not lead to amino acid substitutions,most of which are related to *** cSNPs may prove useful for their potential links to functional cSNPs via linkage disequilibrium *** have selected 48 cSNPs located in the coding regions of 25 genes to construct the cSNP *** genes are harbored in the high frequency loss regions of the chromosome 1p and 8p and related with apoptosis,cell cycles,signal transduction,oncogene,tumor suppressor genes and so *** of the cSNPs can lead to amino acid substitutions except TP73(rs1801174).The PCR products amplified from 31 hepatocellular carcinoma(HCC)specimens were labeled with Dig-dUTP and then hybridized with the cSNP *** results showed that there was no hybridization signal when there was more than one site of mutation in the amplification sequence,indicating that the cSNP chip had a high *** statistic data of the SNP(MT,homozygous and HT,heterozygous)in the HCC patients with different phenotypes(HBV+/-,differentiation stage,family history positive or negative,tumor size)indicated that the number of MT was distinctly different between patients with positive HBV and negative *** MT and HT numbers of all the 48 cSNPs were significantly different between low differentiation and high differentiation HCC *** numbers of MT and HT were not different between positived and negative family history groups and between tumor size3 cm and≤3 cm *** study results provided useful information for understanding the molecular mechanisms of HCC development.