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Mechanism by which Rab5 promotes regeneration and functional recovery of zebrafish Mauthner axons

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Neural Regeneration Research 2025年第6期20卷 1816-1824页

作者： Jiantao Cui Yueru Shen Zheng song Dinggang Fan Bing Hu Center for Advanced Interdisciplinary Science and Biomedicine of IHM Division of Life Sciences and MedicineUniversity of Science and Technology of ChinaHefeiAnhui ProvinceChina

Rab5 is a GTPase protein that is involved in intracellular membrane trafficking. It functions by binding to various effector proteins and regulating cellular responses, including the formation of transport vesicles and their fusion with the cellular membrane. Rab5 has been reported to play an important role in the development of the zebrafish embryo;however, its role in axonal regeneration in the central nervous system remains unclear. In this study, we established a zebrafish Mauthner cell model of axonal injury using single-cell electroporation and two-photon axotomy techniques. We found that overexpression of Rab5 in single Mauthner cells promoted marked axonal regeneration and increased the number of intra-axonal transport vesicles. In contrast, treatment of zebrafish larvae with the Rab kinase inhibitor CID-1067700markedly inhibited axonal regeneration in Mauthner cells. We also found that Rab5 activated phosphatidylinositol 3-kinase(PI3K) during axonal repair of Mauthner cells and promoted the recovery of zebrafish locomotor function. Additionally, rapamycin, an inhibitor of the mechanistic target of rapamycin downstream of PI3K, markedly hindered axonal regeneration. These findings suggest that Rab5 promotes the axonal regeneration of injured zebrafish Mauthner cells by activating the PI3K signaling pathway.

关键词： axonal regeneration Mauthner cell nerve regeneration Rab5 zebrafish

Potential role of tanycyte-derived neurogenesis in Alzheimer's disease

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Neural Regeneration Research 2025年第6期20卷 1599-1612页

作者： Guibo Qi Han Tang Jianian Hu Siying Kang song Qin Department of Anatomy Histologyand EmbryologySchool of Basic Medical SciencesFudan UniversityShanghaiChina State Key Laboratory of Medical Neurobiology and MOE Frontiers Center for Brain Science Fudan UniversityShanghaiChina

Tanycytes, specialized ependymal cells located in the hypothalamus, play a crucial role in the generation of new neurons that contribute to the neural circuits responsible for regulating the systemic energy balance. The precise coordination of the gene networks controlling neurogenesis in naive and mature tanycytes is essential for maintaining homeostasis in adulthood. However, our understanding of the molecular mechanisms and signaling pathways that govern the proliferation and differentiation of tanycytes into neurons remains limited. This article aims to review the recent advancements in research into the mechanisms and functions of tanycyte-derived neurogenesis. Studies employing lineage-tracing techniques have revealed that the neurogenesis specifically originating from tanycytes in the hypothalamus has a compensatory role in neuronal loss and helps maintain energy homeostasis during metabolic diseases. Intriguingly,metabolic disorders are considered early biomarkers of Alzheimer's disease. Furthermore,the neurogenic potential of tanycytes and the state of newborn neurons derived from tanycytes heavily depend on the maintenance of mild microenvironments, which may be disrupted in Alzheimer's disease due to the impaired blood–brain barrier ***, the specific alterations and regulatory mechanisms governing tanycyte-derived neurogenesis in Alzheimer's disease remain unclear. Accumulating evidence suggests that tanycyte-derived neurogenesis might be impaired in Alzheimer's disease, exacerbating neurodegeneration. Confirming this hypothesis, however, poses a challenge because of the lack of long-term tracing and nucleus-specific analyses of newborn neurons in the hypothalamus of patients with Alzheimer's disease. Further research into the molecular mechanisms underlying tanycyte-derived neurogenesis holds promise for identifying small molecules capable of restoring tanycyte proliferation in neurodegenerative diseases. This line of investigation could pro

关键词： Alzheimer's disease blood–brain barrier ependymoglial cells hypothalamus metabolic diseases neural stem cells neurogenesis neuroinflammatory diseases neurons tanycyte

Numerical investigation of separation-induced transition in a lowpressure turbine cascade in a low-disturbance environment

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Science China(Physics,Mechanics & Astronomy) 2020年第6期63卷 66-82页

作者： Si-ya Jiang song Fu School of Aerospace Engineering Tsinghua UniversityBeijing 100084China

In this paper,the separation-induced transition in an LPT(low-pressure turbine)cascade is investigated at low Reynolds number with DNS(direct numerical simulation).The transition process is accurately predicted giving good agreements between the DNS and experimental *** illustrate the secondary instability of separation-induced transition in a low-disturbance environment,the results are comprehensively analyzed in both Fourier space and physical *** is illustrated that the effect of hyperbolic instability dominates around the saddle point of hyperbolic *** instability mechanism is responsible for the emergence of the streamwise vortices in the braid *** and intensified because of the“stretching”effect of the background flow,these vortices become the most noticeable characteristic of the flow *** modes of small spanwise wavelength are excited in the braid region,so as some low-frequency *** elliptical instability plays a minor role than hyperbolic *** is also observed that the fundamental mode with a larger spanwise wavelength is unstable in the vortex core which is associated with the deformation of the vortex core via elliptical *** is no convincing evidence for the existence of subharmonic instability.

关键词： separation direct numerical simulation transition to turbulence

Discordant patterns of genetic variation between mitochondrial and microsatellite markers in Acanthogobius ommaturus across the coastal areas of China

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Acta Oceanologica Sinica 2023年第4期42卷 72-80页

作者： Chenyu song Zhong Tu na song Key Laboratory of Mariculture(Ocean University of China) Ministry of EducationQingdao 266003China Shandong Fisheries Development and Resources Conservation Center Yantai 264003China

Acanthogobius ommaturus, which belongs to the family Gobiidae, is a euryhaline and demersal fish that is widely distributed in the coastal areas, harbors, and estuaries of China, D. P. R. Korea and Japan. In this study, the genetic diversity and genetic structure of five geographical populations of A. ommaturus was assessed using the mitochondrial hypervariable region gene and microsatellite markers. The results of the two genetic markers indicated that the A. ommaturus populations had a high level of genetic diversity. The mitochondrial marker detected weak genetic differentiation among populations, and the Neighbor-Joining tree showed that there was no obvious pedigree branches and geographic structure as well. However, population of Zhoushan showed significant genetic differentiation with other populations by microsatellite markers. The population of *** has not experienced bottleneck effect recently. We speculated that the Pleistocene climate change and juvenile fish dispersal played an important role in the population differentiation of A. ommaturus.

关键词： mitochondrial markers microsatellite markers genetic diversity genetic structure Acanthogobius ommaturus

利用DGGE法研究不同种植体系中根际微生物群落结构

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生物学杂志 2006年第5期23卷 12-15,4页

作者：宋亚娜包兴国李隆张福锁郑伟文中国农业大学资源环境学院北京100094 甘肃农科院土壤肥料研究所兰州730000 福建农科院生物技术研究所福州350003

利用DGGE技术研究不同间作和轮作种植体系对作物根际细菌和真菌群落结构的影响。运用16SrDna和18SrDna特异引物对,将土壤中提取的总Dna进行PCR扩增后,通过DGGE技术对PCR产物进行分析,结果表明:玉米-蚕豆轮作对蚕豆根际细菌和真菌群落结... 详细信息

利用DGGE技术研究不同间作和轮作种植体系对作物根际细菌和真菌群落结构的影响。运用16SrDna和18SrDna特异引物对,将土壤中提取的总Dna进行PCR扩增后,通过DGGE技术对PCR产物进行分析,结果表明:玉米-蚕豆轮作对蚕豆根际细菌和真菌群落结构影响明显,二者都与单作蚕豆有较大差异;小麦/蚕豆间作明显改变两种作物根际细菌群落结构和蚕豆根际真菌群落结构;玉米/蚕豆间作明显改变玉米根际细菌、真菌群落结构和蚕豆根际真菌群落结构。

关键词： DGGE 细菌群落结构真菌群落结构间作轮作

生防菌BC2001对番茄根结线虫的生防研究

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武夷科学 2006年第1期22卷 45-48页

作者：林智敏宋亚娜林营志林捷郑伟文福建省农业科学院生物技术研究所福建福州350003

人工培养番茄根结线虫茎部结瘤,通过盆钵、电镜扫描试验直观评估BC2001菌的生防能力。结果表明,BC2001菌能使茎结瘤消减,且植株生长良好;从扫描电镜上看,BC2001菌可以在结瘤中定殖,并附着在虫体上,同时造成虫体缢缩死亡。

关键词：生物防治生防菌BC2001 番茄根结线虫

Recent Advances in Fibrous Materials for Hydroelectricity Generation

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nano-Micro Letters 2025年第2期17卷 109-133页

作者： Can Ge Duo Xu Xiao Feng Xing yang Zheheng song Yuhang song Jingyu Chen Yingcun Liu Chong Gao Yong Du Zhe Sun Weilin Xu Jian Fang College of Textile and Clothing Engineering Soochow UniversitySuzhou 215123People’s Republic of China national Engineering Laboratory for Modern Silk Soochow UniversitySuzhou 215123People’s Republic of China State Key Laboratory of New Textile Materials and Advanced Processing Technologies Wuhan Textile UniversityWuhan 430200People’s Republic of China Institute of Functional nano&Soft Materials(FUNSOM) Soochow UniversitySuzhou 215123People’s Republic of China Department of Electrical and Computer Engineering University of MassachusettsAmherstMA 01003USA Department of Materials Imperial College LondonSouth Kensington CampusLondon SW72AZUK College of Textile Science and Engineering Zhejiang Sci-Tech UniversityHangzhou 310018People’s Republic of China School of Materials Science and Engineering Shanghai Institute of Technology100 Haiquan RoadShanghai 201418People’s Republic of China

Depleting fossil energy sources and conventional polluting power generation pose a threat to sustainable *** generation from ubiquitous and spontaneous phase transitions between liquid and gaseous water has been considered a promising strategy for mitigating the energy *** materials with unique flexibility,processability,multifunctionality,and practicability have been widely applied for fibrous materials-based hydroelectricity generation(FHG).In this review,the power generation mechanisms,design principles,and electricity enhancement factors of FHG are first ***,the fabrication strategies and characteristics of varied constructions including 1D fiber,1D yarn,2D fabric,2D membrane,3D fibrous framework,and 3D fibrous gel are ***,the advanced functions of FHG during water harvesting,proton dissociation,ion separation,and charge accumulation processes are analyzed in ***,the potential applications including power supply,energy storage,electrical sensor,and information expression are also ***,some existing challenges are considered and prospects for future development are sincerely proposed.

关键词： Hydroelectricity Fibrous material Streaming potential Ion diffusion

Effect of Surface Roughness in Micro-nano Scale on Slotted Waveguide Arrays in Ku-band

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Chinese Journal of Mechanical Engineering 2017年第3期30卷 595-603页

作者： na LI Peng LI Liwei song key laboratory of electronic equipment structure design of ministry of education xidian universityXi'an 710071China

Modeling of the roughness in micro-nano scale and its influence have not been fully investigated, however the roughness will cause amplitude and phase errors of the radiating slot, and decrease the precision and efficiency of the SWA in Ku-band. Firstly, the roughness is simulated using the electromechanical coupled（EC） model. The relationship between roughness and the antenna＇s radiation properties is obtained. For verification, an antenna proto- type is manufactured and tested, and the simulation method is introduced. According to the prototype, a contrasting experiment dealing with the flatness of the radiating plane is conducted to test the simulation method. The advantage of the EC model is validated by comparisons of the EC model and two classical roughness models （sine wave and fractal function）, which shows that the EC model gives a more accurate description model for roughness, the maxi- mum error is 13%. The existence of roughness strongly broadens the beamwidth and raises the side-lobe level of SWA, which is 1.2 times greater than the ideal antenna. In addition, effect of the EC model＇s evaluation indices is investigated, the most affected scale of the roughness is found, which is 1/10 of the working wavelength. The proposed research provides the instruction for antenna designing and manufacturing.

关键词： Slotted waveguide arrays - Roughness model Micro/nano-scale Amplitude and phase errors Radiationcharacteristics

一种适用于高通量基因分型的水稻种子切片Dna制备技术

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福建农业科技 2023年第10期54卷 40-47页

作者：林旻杨绍华林艳王月胡太蛟宋亚娜潘雷博陈在杰福建省农业科学院生物技术研究所/福建省农业遗传工程重点实验室福建福州350003 福建农林大学生命科学学院福建福州350002

建立一种高质量、低成本的水稻种子切片Dna制备方法,用于育种群体的高通量基因分型。通过对比TPS法提取水稻叶片Dna及96孔板结合磁珠法提取切片种子Dna的纯度和浓度,进一步采用STARP(Semi-thermal asymmetric PCR)标记检测验证96孔板结... 详细信息

建立一种高质量、低成本的水稻种子切片Dna制备方法,用于育种群体的高通量基因分型。通过对比TPS法提取水稻叶片Dna及96孔板结合磁珠法提取切片种子Dna的纯度和浓度,进一步采用STARP(Semi-thermal asymmetric PCR)标记检测验证96孔板结合磁珠法提取水稻切片种子Dna的质量。结果表明:96孔板结合磁珠法提取水稻种子切片的Dna质量和纯度优于TPS法提取水稻叶片的Dna质量;STARP标记检测验证结果显示96孔板结合磁珠法提取水稻切片种子Dna可适用于GS3、Wx的STARP标记基因分型,其鉴定结果与测序结果一致。研究显示96孔板结合磁珠法可以从微量水稻种子切片中提出高回收率、高纯度、高完整度、无PCR抑制物的Dna用于高通量STATP法基因单倍型分析,可获得一种96孔板结合磁珠法提取Dna的高通量基因分型操作流程。

关键词：水稻种子切片 96孔板结合磁珠法高通量基因分型 STARP技术

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