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中国植物病理学会2019年学术年会

作者： Ren Yafeng An Xiaoli Li Dongxue Bao xingtao Jiang Shilong Wang Delu song Baoan Chen Zhuo State Key Laboratory Breeding Base of Green Pesticide and Agricultural Bioengineering Guizhou University College of Forestry Guizhou University College of Agriculture Guizhou University

Tea leaf spot disease mainly damaged young leaves and shoot of tea（Camellia sinensis）and led to a huge loss of the production of tea in Kaiyang county,Guizhou *** order to identify the causal agent of this disease,we isolated and identified the *** morphologies of the colony on PDA were undulating,with regular margin and the aerial mycelia represented woolly-cottony and pure *** conidiomata containing black conidial masses were formed on the aerial *** cells were in clusters,simple filiform,hyaline and *** represented fusiform,4-septate,measured 24.99±2.10 μm（20.25-28.52 μm） x 6.00±0.70 μm（4.83-8.56 μm）,with 3-4 apical appendages,and the three median cells were brown,whereas basal cells were *** genes of translation elongation factor 1-alpha（EFl）,the internal transcribed spacer（ITS）,28 S nrDNA（LSU） and beta-tubulin（TUB） were amplified and *** parsimony phylogenetic analysis based on concatenated sequences of combined EF1（1-356）,ITS（357-969）,LSU（970-1895） and TUB（1896-2426） indicated that the strain GZKY2018 AXL4 D was identical to reference strain Pesudopestalotiopsis theae MFLUCC12-0055,and the clade was supported by 97.3% bootstrap *** fulfill the Koch’s postulate,wounded tea leaves were inoculated with actively growing mycelia plugs on *** light brown spots were formed on inoculation site after 1-2 d of *** our knowledge,this is the first report of *** causing tea leaf spot in this region,and this work will help the management of disease in the field.

关键词： Pseudopestalotiopsis theae identification pathogenicity test

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异种材质堆焊渗透裂纹和气孔形成机理

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焊接学报 2011年第6期32卷 97-100,118页

作者：苏允海唐兴涛宋兴奎刘政军沈阳工业大学材料科学与工程学院沈阳110178

将铝青铜和紫铜粉末在低碳钢上进行等离子弧堆焊,通过不同堆焊工艺的对比试验,总结出堆焊工艺对渗透裂纹和气孔的影响规律.结果表明,焊接热输入越大,产生的渗透裂纹倾向越大,气孔几率增加.渗透裂纹的产生与应力状态、大小和堆焊粉末的... 详细信息

将铝青铜和紫铜粉末在低碳钢上进行等离子弧堆焊,通过不同堆焊工艺的对比试验,总结出堆焊工艺对渗透裂纹和气孔的影响规律.结果表明,焊接热输入越大,产生的渗透裂纹倾向越大,气孔几率增加.渗透裂纹的产生与应力状态、大小和堆焊粉末的化学成分以及母材原始状态有关.低熔点共晶体和微裂纹是渗透裂纹形成的根本原因,而铜合金的液态润湿铺展和拉伸应力的作用是渗透裂纹扩展的诱因.只要合理选择焊接工艺和措施,可以避免或减少低碳钢钢板上堆焊铜合金时产生的渗透裂纹和气孔.

关键词：渗透裂纹气孔铜钢合金等离子弧堆焊

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维普期刊数据库

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The Correlative Study between the Four Tea Diseases and Endophytes in Tea Tree

The Correlative Study between the Four Tea Diseases and Endo...

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中国植物病理学会2017年学术年会

作者： Bao xingtao Zhao Xiaozhen Li Dongxue Ren Yafeng Li Xiangyang Wang Yong Yu Haiyou Yang song Chen Zhuo song Baoan Key Laboratory of Green Pesticide and Agricultural Bioengineering Ministry of EducationGuizhou University Agricultural College Guizhou University

The endophytes of tea tree play an important role during the occurrence and development of the tea *** present,there has no research report about the correlations study between the tea diseases and *** this study,the samples of tea blister blight,tea white scab,tea round red spot and tea anthracnose were collected in tea garden from Meitan,Wuchuan,Dejiang,Shiqian,Duyun,Weng’an County in Guizhou Province and Anxi County in Fujian Province in 2016./7 S gene for the pathogens for four tea diseases and specific ITS gene for three endophytes was amplified and *** results indicated that Trichoderma *** in tea blister blight,tea white scab and tea round red spot,Guignardia mangiferae exists in tea blister blight,and Trichoderma longibrachiatum exists in tea blister blight,tea white scab and tea *** addition,two fungus of *** combined with *** *** was simultaneously detected from tea blister blight,two fungus of *** combined with *** simultaneously detected from tea white scab,and only endophyte was detected from tea *** study provided apreliminary implication for the interaction relation between tea diseases and endophytes,and was worthy to further be studied in future.

关键词： Tea diseases Endophytes Identification Correlative study

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A Pathogenic Fungus Phoma *** Identified from Tea Leaf Spot Disease in Shiqian County,Guizhou Province,China

A Pathogenic Fungus Phoma *** Identified from Tea Leaf Spot ...

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中国植物病理学会2017年学术年会

作者： Zhao Xiaozhen Li Dongxue Bao xingtao Duan Changliu Ren Yafeng Li Xiangyang Yu Haiyou Yang song Chen Zhuo song Baoan Key Laboratory of Green Pesticide and Agricultural Bioengineering Ministry of EducationGuizhou University

The tea leaf spot disease is always taken place in the region with the characteristics of the low-temperature and high-humidity containing with Shiqian County,Guizhou *** disease always infects the tender leaves and shoots,which lead to the lowest tea quality and the significant production *** identify the pathogen of the tea spot disease,this work isolated and identified the pathogen of this disease from the tea leaves,and determined the pathogenicity of the pathogen according to Koch’s *** study indicated that the isolated strain of GZSQ-4 represent the features as following;on PDA medium,margin regular of the colonies,aerial mycelium closely growing,white mycelium in the early stage;then gradually change to grey brown,black brown in the center,with white margin in the latter stage;reverse brown to black brown near the center,with the white *** OA medium,margin regular of the colonies,aerial mycelium woolly,pale brown to brown near the center,and produce a lot of black *** the optical microscope and SEM indicated that the septate mycelium contains the inclusion granule;Pycnidia represent subglobose,glabrous,thin-walled,solitary or confluent,50 190μm,1 or 2 ostioes with a neck;Conidia represent ellipsoidal or ovoid,thin-walled,smooth,hyaline,aseptate,(2) μm×(1 .5) μm,with 0 guttules in two polar *** genes of ITS,β-tubulin and LSU were further amplified,purified and sequenced,then the phylogenetic trees were constructed using MEGA6.0 software and Neighbor-joining *** results indicated that the strainof GZSQ-4 was closely related to the strains of Phoma ***-2014 b(LC 1633) and Didymel-1 a bellidis(CBS 714.85).Integrated with the above results,the pathogen isolated by our research group are preliminarily identified to Phoma sp..

关键词： Phoma sp Tea leaf spot disease Pathogenic fungus

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声门下支气管源性囊肿1例

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中华耳鼻咽喉头颈外科杂志 2024年第2期59卷 177-179页

作者：宋柳杨慧景星涛张宏慧四川大学华西医院耳鼻咽喉-头颈外科成都610041

患者女,36岁,因“咽部异物感1个月余,呼吸困难15 d”就诊。门诊电子纤维喉镜提示,声门下一半球形肿物隆起,突入并堵塞气道90%,遂紧急行气管切开术。术前颈部增强CT见C6平面喉咽右侧壁软组织结节影凸向气管腔,边缘见低密度影。急诊在全... 详细信息

患者女,36岁,因“咽部异物感1个月余,呼吸困难15 d”就诊。门诊电子纤维喉镜提示,声门下一半球形肿物隆起,突入并堵塞气道90%,遂紧急行气管切开术。术前颈部增强CT见C6平面喉咽右侧壁软组织结节影凸向气管腔,边缘见低密度影。急诊在全身麻醉下行经口支撑喉镜下CO 2激光声门下肿物切除术。病理标本大体检查示,肿瘤大小2.0 cm×1.0 cm,剖开内为黏稠胶冻样组织,囊壁厚0.2 cm,术后病理诊断为支气管源性囊肿。患者于术后第2天堵管,术后3 d出院,出院1年复查未见肿物复发,预后良好。

关键词：咽部异物感支气管源性囊肿肿物切除术病理标本声门下预后良好结节影低密度影

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The Identification of a Pathogenic Fungus from Gloeosporium theae-sinensis Miyake in Yuqing County,Guizhou Province,China

The Identification of a Pathogenic Fungus from Gloeosporium ...

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中国植物病理学会2017年学术年会

作者： Li Dongxue Zhao Xiaozhen Bao xingtao Ren Yafeng Li Xiangyang Yu Haiyou Tan Xiaofeng Chen Zhuo song Baoan Key Laboratory of Green Pesticide and Agricultural Bioengineering Ministry of EducationGuizhou University

Gloeosporium Theae-Sinensis Miyake is an important tea disease,which mainly damage the mature *** disease can cause an amount of fallen leaves when it occurs *** disease mainly occurs in the season of high humidity and rainy day,and then become a main disease of tea in Guizhou *** work isolated the pathogen from the diseased leaves from the tea,and determined the pathogenicity of the pathogen according to Koch’s *** on this,we obtained 3 strains of GZYQ-1,GZYQ-2,and ***-2 strain was further studied and the results indicated that margin regular of the colonies with white and villiform,aerial mycelium developed near the center,along with being upheaved;reverse faint yellow near the center,with the white margin on PDA medium in the early stage;The colonies with light color near the center,and deep color along the *** the area of the colonies with faint yellow being gradually increased,and deep color near the center,light color along the margin,with few black *** conidium did not form on PDA *** genes of ITS and β-tubulin were amplified and sequenced,and the sequence alignment was conducted using BLAST ***-2 strain and the strains with high identity were used to construct the phylogenetic trees using MEGA 6.0 software according to Neighbor-Joining *** results indicated that GZYQ-2 is closely related to Colletotrichum ignotum isolate Coll（JX515292.1） and ***（GU174550.1）for ITS ***-2 and ***（AY245019.1） is converged to a branch,closely related to *** CMM4243（KU213604.1）.

关键词： Camellia sinensis Gloeosporium theae-sinensis Miyake Identification

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Investigating the antifungal activity and mechanism of a microbial pesticide Ningnanmycin against Phoma segeticola ***

Investigating the antifungal activity and mechanism of a mic...

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中国植物病理学会2019年学术年会

作者： Li Dongxue Wang Xue Yin Qiaoxiu Ren Yafeng Bao xingtao Dharmasena Dissanayake-saman-pradeep Wu Xian song Baoan Chen Zhuo State Key Laboratory Breeding Base of Green Pesticide and Agricultural Bioengineering Guizhou University

Phoma segeticola *** was isolated from tea leaf spot disease in tea garden,Shiqian county,Guizhou *** leaf spot disease affected seriously the quality and yield of *** present,there are no effective measures of prevention and ***(NNM) is a new cytosine nucleoside peptide antibiotic with high efficiency and low *** method of mycelial growth rate was used to determine the antifungal activity of NNM against *** *** strain GZSQ-4,with EC of 1 287.54 U/*** order to further study the mechanism of NNM on GZSQ-4,the mycelium treated with NNM at the dosage of EC for 1 h and 14 h was observed by optical microscope,scanning electron microscopy and transmission electron microscopy,*** was found that the mycelium swelled,the mycelial cytoplasmic density decreased,the granular content appeared and the number of mitochondria granules *** GZSQ-4 being treated by NNM at the dosage of EC for 1 h,RNA-seq showed that 1 363 differentially expressed genes(DEGs) were identified with 743 DEGs upregulated and 620 DEGs *** GO enrichment analysis,the DEGs were most enriched in the structural constituent of ribosome of molecular function,ribosome of cellular component and the oxidation-reduction process of biological process including 56,38 and 116 DEGs,respectively(P<0.05).By KEGG enrichment analysis,the DEGs were most enriched in the pathway of ribosome including 58 *** selected randomly DEGs from treatment group of NNM for 1 h were verified by *** results showed that the expression level of DEGs was consistent with the result of *** results indicated that NNM may affect the processes of translation of *** study will provide useful information for management of tea leaf spot caused by *** *** and increase our understanding of action mechanism of NNM against the pathogen.

关键词： tea disease Phoma segeticola var.camellia Ningnanmycin antifungal activity action mechanism

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河北平原土壤有机碳含量的变化

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地质学报 2011年第9期85卷 1528-1535页

作者：栾文楼宋泽峰李随民崔邢涛石家庄经济学院河北050031

应用1979年第二次土壤普查数据和2004年多目标地球化学调查结果及其它资料,利用网格单元分别计算了1979年和2004年河北平原0~0.2m和0~1.8m的不同土壤类型的有机碳储量。结果表明河北平原土壤有机碳含量在25年间持续增长,2004年浅层和深... 详细信息

应用1979年第二次土壤普查数据和2004年多目标地球化学调查结果及其它资料,利用网格单元分别计算了1979年和2004年河北平原0~0.2m和0~1.8m的不同土壤类型的有机碳储量。结果表明河北平原土壤有机碳含量在25年间持续增长,2004年浅层和深层土壤平均有机碳储量比1979年有了分别增加了24.25%和31.39%。水分含量、土壤母质和质地等是影响土壤碳贮存能力的因素。含水量和粘粒含量较高的水稻土、沼泽土、砂姜黑土等土壤类型碳储能力强,反之含水低、粘粒含量低的土壤类型如风沙土和新积土等则储碳能力较弱。植被的破坏、土壤水分的流失会导致高水分含量的土壤类型的有机碳储量降低。在本研究中表现为绝大多数土壤类型的平均有机碳储量升高,但是表层的棕壤、沼泽土、滨海盐土和深层的棕壤、沼泽土等土壤类型发生了降低。

关键词：有机碳储量土壤河北平原

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Draft genome resource of the tea leaf spot pathogen Didymella segeticola

Draft genome resource of the tea leaf spot pathogen Didymell...

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中国植物病理学会2019年学术年会

作者： Li Dongxue Ren Yafeng Wang Xue Yin Qiaoxiu Bao xingtao Dharmasena Dissanayake-saman-pradeep Wu Xian song Baoan Chen Zhuo State Key Laboratory Breeding Base of Green Pesticide and Agricultural Bioengineering Guizhou University College of Agriculture Guizhou University

The fungal pathogen Didymella segeticola（Basionym:Phoma segeticola） causes leaf spot on tea（Camellia sinensis）,which leads to a loss in tea leaf production in Guizhou Province,*** isolate GZSQ-4 was sequenced on Illumina Hi-Seq platform,generating 53,530,422 raw *** Illumina reads were trimmed,corrected and filtered.A total of 51,270,673 clean reads passed the quality control criteria and were used in subsequent analyses of *** isolate ***,a Pacific Biosciences（PacBio） RS library was prepared using G-tubes and sequenced using single-molecule real-time cells,generating PacBio *** reads were further filtered,generating PacBio subreads,with 1 489 032 total *** largest length was 63 621 bp,and the N50,N90 and average lengths were 10 453 bp,5 327 bp and 7 939 bp,*** genome size was～36.7 Mbp based on read sequence data being processed with the condition of 21-mers using the K-mer statistical *** whole-genome assembly was～33.4 Mbp with a scaffold N50 value of-2.3 *** total,10 893 genes were predicted using the Non-redundant,Gene Ontology,Clusters of Orthologous Groups,Kyoto Encyclopedia of Genes and Genomes,and SWISS-PROT *** genome sequence has been deposited in the NCBI Sequencing Read Archive database and can be accessed with the gene accession SDAV00000000（BioProject:PRJNA516041;BioSample:SAMN10768991;SRA accession:SRR8492863）.The whole-genome sequence of *** will provide a resource for future research on host-pathogen interactions,determination of trait-specific genes,pathogen evolution and plant-host adaptation mechanisms.

关键词： whole genome sequences fungal pathogen Didymella segeticola leaf spot

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Comparative transcriptome profiling of mRNA and lncRNA related to tea leaves infected by Phoma segeticola ***

Comparative transcriptome profiling of mRNA and lncRNA relat...

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中国植物病理学会2019年学术年会

Non-coding RNAs(ncRNAs) such as long non-coding IncRNA have been reported to play important roles in the regulation of many biological *** rapid development of omics sequencing technology has promoted the identification of thousands of lncRNAs in plant species,but the role of IncRNAs in plant-pathogen interactions remains largely *** identified significant differentially expressed genes(DEGs) andlncRNAs(DELs),and then construct lncRNA-mRNA networks using comparative transcriptome analysis of the leaves of tea plant(Camellia sinensis) infected and uninfected by Phoma segeticola ***,coupled with bioinformatics.A total of 1615 DEGs and 561 DELs(including 146 up-regulated IncRNAs and 415 down-regulated lncRNAs,P<0.05 and I log≥1) were identified between tea leaves infected and uninfected by *** *** network of co-localization was constructed for the analysis of the interaction of DEGs and *** DEGs may be cis-regulated by DELs were enriched in 10 terms containing with NADP binding and sucrose alpha-glucosidase activity,et al(P<0.05).By the enrichment analysis of KEGG,4 DELs were most enriched in the pathway of biosynthesis of amino acids(P<0.05).Integrated the analysis of the data of DEGs and DELs,6 pairs of significant co-expression pairs were screened,with 5 positively correlated pairs(COR≥0.7) and 1 negatively correlated pair(COR≤-0.7).As the differentially expressed trends of lncRNAs,IncRNA118096(MSTRG.70289.1) and lncRNA20211(MSTRG.12101.1) may play crucial roles in plant defense *** summary,the present study extends the IncRNA database of the tea leaves and these IncRNAs may provide reference on the study of plant-pathogen interactions in the future.

关键词： tea leaves Phoma segeticola var.camelliae lncRNA co-expressing

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