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Agricultural Science & Technology 2011年第7期12卷 980-984,996页

作者： song xin-wei lin jian-rong wu ming-guo State Key Laboratory of Rice Biology China National Rice Research Institute Hangzhou 310006

From the view of methodology for genetic improvement ofJaponicacytoplasmic male sterile(CMS) lines,we introduced the breeding process of the dwarf-abortive type(DA type)JaponicaCMS line Chunjiang 95A with wide compatibility,and further investigated the biological characteristics of the DA type Chunjiang 95A and its isonuclear alloplasmic CMS line Dian-I type Chunjiang *** results show that DA type Chunjiang 95A is a japonicalinous CMS line and mainly typical abortive,which is better in fertility stability than stained abortive typeJaponica CMS *** DA type Chunjiang 95A is featured with high stigma exsertion,good flowering habit,high outcrossing rate and wide *** recoverability differs from stained abortive typeJaponicaCMS ***,we discussed genetic improvement of the typical abortive type JaponicaCMS lines and the application prospects of heterosis.

关键词： Wide compatibility Japonica rice CMS line Stigma exsertion Breeding

Hepatocellular adenoma with malignant transformation in male patients with non-cirrhotic livers

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Chinese Journal of Cancer 2015年第5期34卷 217-224页

作者： song-lin An Li-ming Wang wei-Qi rong Fan wu wei Sun wei-Bo Yu Li Feng Fa-Qiang Liu Fei Tian jian-Xiong wu Department of Abdominal Surgery Cancer Institute and HospitalChinese Academy of Medical Sciences and Peking Union Medical College Department of Pathology Cancer Institute and HospitalChinese Academy of Medical Sciences and Peking Union Medical College

Introduction:Hepatocellular adenomas(HCAs),with a risk of malignant transformation into hepatocellular carcinoma(HCQ,classically develop in young women who are taking oral *** is now clear that HCAs may also occur in ***,it is rarely reported that HCAs with malignant transformation occur in male patients with non-cirrhotic *** study aimed to characterize the malignancy of HCAs occurring in male ***:All patients with HCAs with malignant transformation who underwent hepatectomy at the Cancer Institute and Hospital,Chinese Academy of Medical Sciences and Peking Union Medical College between January 1,1999 and December 31,2011 were enrolled in the *** clinical characteristics as well as radiologic and pathologic data were ***:HCAs with malignant transformation were observed in 5 male patients with non-cirrhotic livers,but not in female *** alpha-fetoprotein(AFP) levels were higher in patients with HCAs with malignant transformation than in patients with HCAs without malignant *** diameters of the tumors with malignant transformation were larger than 5 cm in 3 cases and smaller than 5 cm in 2 *** 5 patients were all alive without recurrence by the end of the study *** disease-free survival times of the 5 patients were 26,48,69,69,and 92 ***:Our results indicate that resection would be advised even if the presumptive diagnosis is adenoma smaller than 5 cm in diameter,especially in male patients.

关键词：恶性转化肝硬化患者胞腺恶性肿瘤化合物肝细胞癌肿瘤医院

Breeding of New Japonica-indica Hybrid Rice Combination Chunyou 59

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Agricultural Science & Technology 2011年第8期12卷 1139-1140,1143页

作者： wu ming-guo lin jian-rong song xin-wei RUAN Guan-hai China National Rice Research Institute Hangzhou 310006 Zhejiang Academy of Agricultural Sciences Hangzhou 310021

Chunyou 59 is a japonica-indica hybrid rice combination developed from Chunjiang 16A(a late japonica CMS line with dwarfness and early anthesis time)and CH59(an indicaclinous wide compatibility restorer line).It has the characteristics of strong heterosis,strong stem,good lodging resistance,large panicles,good cold tolerance,high yield and wide *** was registered and released by jiangxi Provincial Crop Variety Appraisal Committee in March *** breeding procedure,characteristics,major techniques of cultivation and seed production of Chunyou 59 were introduced in the paper.

关键词： Japonica-indica hybrid rice Chunyou 59 Lodging resistance Breeding

A highly efficient pathway to recover gold from acid aqueous solution by using an amidoxime-functionalized UHMWPE fiber

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Rare Metals 2019年第11期38卷 1105-1112页

作者： Yu-lin Liang Chan Jin jiang-Tao Hu Zhen Liu Yan Li jian-rong Zeng guo-Zhong wu Xiang-Jun wei Shanghai Institute of Applied Physics Chinese Academy of SciencesShanghai 201800China University of Chinese Academy of Sciences Beijing 100049China School of Physical Science and Technology ShanghaiTech UniversityShanghai 201210China

A new amidoxime-functionalized ultrahigh molecular weight polyethylene fibrous adsorbent(UHMWPEg-PAO) was used for the recovery of Au(III) from acid aqueous *** maximum experimental absorption capacity of UHMWPE-g-PAO fiber was found to be approximately 220.0 mg·g^-*** adsorption on UHMWPE-g-PAO was characterized by synchrotron radiation-based microcomputed tomography(SR-μ-CT),transmission electron microscopy(TEM),X-ray absorption near-edge structure spectroscopy(XANES) and synchrotron radiation-based Fourier transform infrared microspectroscopy(SR-FTIR).The SR-μ-CT and TEM results showed the distribution features of gold particles on the *** mechanism of Au(III) recovery from aqueous solutions by UHMWPE-g-PAO was based on the adsorbed Au(III) ions,which were reduced to metallic zero-valent gold(Au(0)) by amino groups(-NH2) in the amidoxime(AO)groups during the adsorption process,as confirmed by highresolution TEM(HRTEM) and XANES analyses.X-ray diffraction(XRD) results indicated that the incineration ashes contained metallic zero-valent *** conclusion,this amidoxime-functionalized fibrous adsorbent is a promising material for recovering gold from aqueous solution.

关键词： UHMWPE-g-PAO Adsorption mechanism Gold Reduction

Three-dimensional morphometric analysis for hepatectomy of centrally located hepatocellular carcinoma:A pilot study

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World Journal of Gastroenterology 2015年第15期21卷 4607-4619页

作者： Fei Tian jian-Xiong wu wei-Qi rong Li-ming Wang Fan wu wei-Bo Yu song-lin An Fa-Qiang Liu Li Feng Chao Bi Yun-He Liu Abdominal Surgery Department Cancer Institute and Hospital Peking Union Medical College Chinese Academy of Medical Sciences

AIM: To describe a three-dimensional model(3DM) to accurately reconstruct anatomic relationships of centrally located hepatocellular carcinomas(HCCs).METHODS: From March 2013 to July 2014, reconstructions and visual simulations of centrally located HCCs were performed in 39 patients using a 3D subject-based computed tomography(CT) model with customdeveloped software. CT images were used for the 3D reconstruction of Couinaud's pedicles and hepatic veins, and the calculation of corresponding tumor territories and hepatic segments was performed using Yorktal DMIT software. The respective volume, surgical margin, and simulated virtual resection of tumors were also estimated by this model preoperatively. All patients were treated surgically and the results were retrospectively assessed. Clinical characteristics, imaging data, procedure variables, pathologic features, and postoperative data were recorded and compared to determine the reliability of the ***: 3D reconstruction allowed stereoscopic identification of the spatial relationships between physiologic and pathologic structures, and offered quantifiable liver resection proposals based on individualized liver anatomy. The predicted values were consistent with the actual values for tumor mass volume(82.4 ± 109.1 m L vs 84.1 ± 108.9 m L, P = 0.910), surgical margin(10.1 ± 6.2 mm vs 9.1 ± 5.9 mm, P = 0.488), and maximum tumor diameter(4.61 ± 2.16 cm vs 4.53 ± 2.14 cm, P = 0.871). In addition,the number and extent of portal venous ramifications, as well as their relation to hepatic veins, were visualized. Preoperative planning based on simulated resection facilitated complete resection of large tumors located in the confluence of major vessels. And most of the predicted data were correlated with intraoperative ***: This 3DM provides quantitative morphometry of tumor masses and a stereo-relationship with adjacent structures, thus providing a promising technique for the management of centrally loc

关键词： Centrally located Hepatectomy Hepatocellularcarcinoma Liver Three-dimensional model

An optimized protocol for detecting guard cell specific gene expression by in situ RT-PCR in Brassica rapa

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Horticultural Plant Journal 2022年第3期8卷 311-318页

作者： Yingying song xinlei guo jian wu jianli Liang Runmao lin Zifu Yan Xiaowu Wang College of Horticulture Henan Agricultural UniversityZhengzhou 450002China Institute of Vegetables and Flowers Chinese Academy of Agricultural SciencesBeijing 100081China

It is important to detect specific genes expressed in the guard cells,which control gas exchange and play key roles in response to drought and salt *** to the genetic transformation of Chinese cabbage(Brassica rapa)has not been well developed,in situ RT-PCR is a valuable option for detecting guard cell specific *** reported an optimized protocol of in situ RT-PCR by using an FAMA homologous gene Bra001929 in Brassica *** in Arabidopsis has been verified to be specially expressed in guard *** designed specific RT-PCR primers and optimized the protocol in terms of the(a)reverse transcription time,(b)blocking time,(c)antigen-antibody incubation time,and(d)washing *** approach provides a sensitive and effective in situ RT-PCR method for locating expression in the guard cells in Brassica ***,we proved the guard cell specific expression of Bra001929 in the epidermis indicating its’applicability as a marker gene for guard cells of Brassica rapa.

关键词： Brassica rapa In situ RT-PCR Gene expression Bra001929 Protocol

RAD51 potentiates synergistic effects of chemotherapy with PCI-24781 and cis-diamminedichloroplatinum on gastric cancer

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World Journal of Gastroenterology 2014年第29期20卷 10094-10107页

作者： wei-ling He Yu-Huang Li wei-jian Hou Zun-Fu Ke xin-lin Chen Li-Ya Lu Shi-rong Cai wu song Chang-Hua Zhang Yu-Long He Department of Gastrointestinal and Pancreatic Surgery The First Affiliated HospitalSun Yat-sen University Department of Molecular and Medical Genetics Oregon Health and Science University Otorhinolaryngology Hospital The First Affiliated HospitalSun Yat-Sen University Department of Pathology the First Affiliated HospitalSun Yat-sen University College of Fundamental Medical Science Guangzhou University of Chinese MedicineGuangzhou Higher Education Mega Center Public Health Institute of Health and WellbeingUniversity of Glasgow

AIM:To explore the efficacy of PCI-24781,a broadspectrum,hydroxamic acid-derived histone deacetylase inhibitor,in the treatment of gastric cancer(GC).METHODS:With or without treatment of PCI-24781and/or cis-diamminedichloroplatinum(CDDP),GC cell lines were subjected to functional analysis,including cell growth,apoptosis and clonogenic *** immunoprecipitation and luciferase reporter assays were used to determine the interacting molecules and the activity of the *** in vivo study was carried out in GC xenograft *** culture-based assays were represented as mean±*** tests were used to assess differences across *** pairwise comparisons between tumor weights among treatment groups were made using the Tukey-Kramer method for multiple comparison adjustment to control experimental-wise typeⅠ error *** was set at Pweight in vivo was obtained following PCI-24781 treatment,which synergized with the anticancer effect of ***:These data suggest that RAD51 potentiates the synergistic effects of chemotherapy with PCI-24781 and CDDP on GC.

关键词： Chemotherapy Combination Gastric cancer Histone deacetylase inhibitor Homologous recombination

Excretion and detection of SARS coronavirus and its nucleic acid from digestive system

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World Journal of Gastroenterology 2005年第28期11卷 4390-4395页

作者： xin-wei Wang Qing-xin Kong Zhong Li Nong song Min Jin Chang-Qing Gu Jing Yin guo-rong Ou Fu-Huan Chao Jun-Wen Li Jin-song Li Bei Zhen Xiao-ming wu Wen-Jun Xiao wei wei Min-Nian Wang Gui-Jie Wang Ting-Kai guo Xiu-Mei Zhu wei Yao jian-Feng Li Yao-Hui Qiu Huai-Huan wu Chao Liu Tong-Yu Fang Tianjin Institute of Environment and Health Tianjin 300050 China Institute of Microbiology and Epidemiology Beijing 100072 China Xiao Tang Shan Hospital Beijing 102211 China Bin Yi 309 Hospital of PLA Beijing 100091 China Beijing Institute of Pharmacology and Toxicology Beijing 100850 China Beijing Institute of Basic Medicine Beijing 100850 China

AIM： To study whether severe acute respiratory syndrome coronavirus （SARS-CoV） could be excreted from digestive system.METHODS： Cell culture and semi-nested RT-PCR were used to detect SARS-CoV and its RNA from 21 stool and urine samples, and a kind of electropositive filter media particles was used to concentrate the virus in 10 sewage samples from two hospitals receiving SAILS patients in Beijing in China. RESULTS： It was demonstrated that there was no live SARS-CoV in all samples collected, but the RNA of SARS-CoV could be detected in seven stool samples from SARS patients with any one of the symptoms of fever, malaise, cough, or dyspnea, in 10 sewage samples before disinfection and 3 samples after disinfection from the two hospitals. The RNA could not be detected in urine and stool samples from patients recovered from SARS.CONCLUSION： Nucleic acid of SARS-CoV can be excreted through the stool of patients into sewage system, and the possibility of SARS-CoV transmitting through digestive system cannot be excluded.

关键词： Severe acute respiratory syndrome Nucleic acid Digestive system

A Diagnostic Tool for Identification of Etiologies of Fever of Unknown Origin in Adult Patients

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Current Medical Science 2019年第4期39卷 589-596页

作者： Man-zhi ZHAO Qiu-rong RUAN ming-you xinG Sheng wei Dong XU Zhu-hua wu lin ZHU Ji-ling ZHU Cai-feng ZHENG Shi LIU Zu-jiang YU Jun-ying QI jian-xin song Department of Infectious Diseases Tongji HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430030China Institute of Pathology Tongji HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430030China Department of Epidemiology and Bio statistics School of Public HealthTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430030China Department of Internal Medicine Union HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430022China Department of Infectious Diseases the First Affiliated Hospital of Medical College of Zhengzhou UniversityZhengzhou 450052China

The diagnosis and treatment of fever of unknown origin (FUO) are huge challenges to *** the etiologies of FUO into infectious and non-infectious disease is conducive to clinical physicians not only on making decisions rapidly concerning the prescription of suitable antibiotics but also on further analysis of the final *** order to develop and validate a diagnostic tool to efficiently distinguish the etiologies of adult FUO patients as infectious or non-infectious disease,FUO patients from the departments of infectious disease and internal medicine in three Chinese tertiary hospitals were enrolled retrospectively and *** using polynomial logistic regression analysis,the diagnostic formula and the associated scoring system were *** variables included in this diagnostic formula were from clinical evaluations and common laboratory *** proposed tool could discriminate infectious and noninfectious causes of FUO with an area under receiver operating characteristic curve (AUC) of 0.83,sensitivity of 0.80 and specificity of *** diagnosis tool could predict the infectious and non-infectious causes of FUO in the validation cohort with an AUC of 0.79,sensitivity of 0.79 and specificity of *** results suggested that this diagnostic tool could be a reliable tool to discriminate between infectious and non-infectious causes of FUO.

关键词： fever of unknown origin prediction model diagnostic tool etiology empiric therapy

Anti-tumor effect of 5-aza-2'-deoxycytidine by inhibiting telomerase activity in hepatocellular carcinoma cells

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World Journal of Gastroenterology 2012年第19期18卷 2334-2343页

作者： Shuang-FenTao Chang-song Zhang Xian-ling guo Yun Xu Shan-Shan Zhang jian-Rui song rong Li Meng-Chao wu Li-xinwei Tumor Immunology and Gene Therapy Center Eastern Hepatobiliary Surgery Hospital The Second MilitaryMedical University Shanghai 200438 China Clinical Oncology Laboratory ChangzhouTumor Hospital Medical College of Suzhou University Chang-zhou 213001 Jiangsu Province China

AIM:To investigate the effect of the demethylating reagent 5-aza-2'-deoxycitidine(DAC) on telomerase activity in hepatocellular carcinoma(HCC) cell lines,SMMC-7721 and ***:The related gene expression in cell lines was examined by real-time reverse transcription-polymerase chain reaction and Western blotting *** telomerase activity was examined by telomeric repeat amplification protocol-enzyme-linked immunosorbent assay and DNA methylation was determined by methylation-specific polymerase chain ***:The telomerase activity was significantly reduced in both cell lines treated with DAC,accompanied by downregulation of telomerase reverse transcriptase(hTERT).We also observed the effect of DAC on the methylation status of hTERT promoter and the expression of regulatory genes,such as c-myc,p15,p16,p21,E2F1,and *** methylation status of hTERT promoter could be reversed in SMMC-7721 by DAC,but not in HepG2 ***,p16 expression could be reactivated by demethylation of its promoter,and c-Myc expression was repressed in both cell ***,DAC could enhance the sensitivity to the chemotherapeutic agents,such as cisplatin,by induction of apoptosis of HCC ***:The DAC exerts its anti-tumor effects in HCC cells by inhibiting the telomerase activity.

关键词： 5-aza-2'-deoxycitidine Telomerase Hepa-tocellular carcinoma DNA methylation