An optimized protocol for detecting guard cell specific gene expression by in situ RT-PCR in Brassica rapa

作     者：Yingying Song Xinlei Guo Jian Wu Jianli Liang Runmao Lin Zifu Yan Xiaowu Wang Yingying Song;Xinlei Guo;Jian Wu;Jianli Liang;Runmao Lin;Zifu Yan;Xiaowu Wang

作者机构：College of HorticultureHenan Agricultural UniversityZhengzhou 450002China Institute of Vegetables and FlowersChinese Academy of Agricultural SciencesBeijing 100081China 

出 版 物：《Horticultural Plant Journal》 (园艺学报（英文版）)

年 卷 期：2022年第8卷第3期

页      面：311-318页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 09[农学] 071007[理学-遗传学] 0901[农学-作物学] 090102[农学-作物遗传育种] 

基　　金：This work is supported by the National Natural Science Foundation of China(Grant No.31630068) the Key Laboratory of Biology and Genetic Improvement of Horticultural Crops Ministry of Agriculture China 

主　　题：Brassica rapa In situ RT-PCR Gene expression Bra001929 Protocol 

摘      要：It is important to detect specific genes expressed in the guard cells,which control gas exchange and play key roles in response to drought and salt *** to the genetic transformation of Chinese cabbage(Brassica rapa)has not been well developed,in situ RT-PCR is a valuable option for detecting guard cell specific *** reported an optimized protocol of in situ RT-PCR by using an FAMA homologous gene Bra001929 in Brassica *** in Arabidopsis has been verified to be specially expressed in guard *** designed specific RT-PCR primers and optimized the protocol in terms of the(a)reverse transcription time,(b)blocking time,(c)antigen-antibody incubation time,and(d)washing *** approach provides a sensitive and effective in situ RT-PCR method for locating expression in the guard cells in Brassica ***,we proved the guard cell specific expression of Bra001929 in the epidermis indicating its’applicability as a marker gene for guard cells of Brassica rapa.