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Pollen viability of Polygala paniculata L.(Polygalaceae)using different staining methods

BIOCELL 2012年第3期36卷 143-145页

作者： VIVIANE DAL-SOUTO FRESCURA HAYWOOD DAIL LAUGHINGHOUSE IV THAIS SCOTTI DO CANTO-DOROW solange bosio tedesco Laboratory of Cytogenetics and Genotoxicity Department of BiologyCentro de Ciências Naturais e Exatas(CCNE)Universidade Federal de Santa Maria(UFSM)Avenida Roraimanº1000Cep.97105-900CamobiSanta MariaRio Grande do SulBrasil Centre for Protein Engineering Institute of Chemistry B6University of LiègeBelgium

Polygala paniculata *** a medicinal plant that grows in the Brazilian Atlantic coast,known as‘barba-de-São-João’,‘barba-de-bode’,‘vassourinha branca’,and‘mimosa’.In this study,pollen viability was estimated by three different staining methods:2%acetic orcein,2%acetic carmine,and Alexander’s *** young inflorescences of twenty accessions were collected and fixed in a solution of ethanol:acetic acid(3:1)for 24 hours,then stored in ethanol 70%under *** slides per plant,two for each stain,were prepared by squashing,and 300 pollen grains per slide were *** viability was high(>70%)for most accessions of *** using the Alexander’s stain,which proved the most adequate method to estimate pollen viability.

关键词： pollen grains barba-de-bode acetic orcein acetic carmine Alexander’s stain

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Antiproliferative effect of extracts of Sida rhombifolia L.(Malvaceae)on the Allium cepa cell cycle

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BIOCELL 2014年第1期38卷 7-9页

作者： Giselle de Moura CARPES Viviane Dal-Souto FRESCURA solange bosio tedesco Antonio Carlos Ferreira DA SILVA Thais Scotti do CANTO-DOROW Plant Cytogenetics Laboratory Universidade Federal de Santa Maria(UFSM)Santa MariaRio Grande do Sul(RS)Brazil

Field collected roots of four populations of Sida rhombifolia were used for preparing aqueous de-coctions at two concentrations:4g/L;and 16g/***,we used three groups of six onion(Allium cepa)bulbs for testing each *** were made with all bulbs through the smashing *** in all phases of the cell cycle of *** were *** mitotic index(%of cells in mitosis)was calculated,and the statistical analysis through theχ2 test was carried out at 5%*** results showed that the aqueous extracts of *** have antiproliferative activity at high *** no chromosomal ab-errations were induced by treatments.

关键词： Antiproliferative medicinal plant guanxuma onion.

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Meiotic behavior and pollinic viability in bean cultivars

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BIOCELL 2014年第1期38卷 33-38页

作者： Gracieli DALLA NORA solange bosio tedesco Tamara PASTORI Viviane Dal-Souto FRESCURA Nerinéia Dalfollo RIBEIRO Haywood Dail LAUGHINGHOUSE IV Sidinei JoséLOPES Maria Teresa SCHIFINO-WITTMANN Laboratory of Cytogenetics and Plant Genotoxicity Department of BiologyCenter for Natural and Exact SciencesFederal University of Santa Maria(UFSM)Rio Grande do SulBrazil

The aims of this study were to determine the meiotic behavior and to estimate pollen grains via-bility in bean(Phaseolus vulgaris L.)*** buds were collected during different developmental stages of the Mesoamerican bean cultivars IAPAR 44,Guapo Brilhante,BRS Expedito,BRS Valente,Guateian 6662 and Pérola,and the Andean bean cultivar Iraí,grown in a *** meiotic index was determined by anther squashing of material fixed in absolute ethanol-glacial acetic acid(3:1)and stained with acetic *** meiotic abnormalities were observed and the meiotic indices were high for all cultivars,indicating that the mismatch generated during crosses is not related to any meiotic *** of pollen viability was made by comparing acetic orcein staining ***’s reactive:pollen viability was high in all cultivars with either stain,but was significantly higher when using the acetic orcein stain(>99%).Though some cultivar showed a significantly smaller pollen size,the range of variation among cultivars was low(means’range 51-66μm).

关键词： Acetic orcein Phaseolus vulgaris pollen Alexander’s reactive

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Post-treatment with plant extracts used in Brazilian folk medicine caused a partial reversal of the antiproliferative effect of glyphosate in the Allium cepa test

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BIOCELL 2013年第2期37卷 23-28页

作者： VIVIANE DAL-SOUTO FRESCURA ANDRIELLE WOUTERS KUHN HAYWOOD DAIL LAUGHINGHOUSE IV JUÇARA TEREZINHA PARANHOS solange bosio tedesco Department of Biology Centro de Ciências Naturais e Exatas(CCNE)Universidade Federal de Santa Maria(UFSM)Avenida Roraimanº1000Cep.97105-900CamobiSanta MariaRio Grande do SulBrasil Centre for Protein Engineering Institute of Chemistry B6University of LiègeBelgium.

Species of the genus Psychotria are used for multiple purposes in Brazilian folk medicine,either as water infusions,baths or *** study was aimed to evaluate the genotoxic and antiproliferative effects of infusions of Psychotria brachypoda and *** on the Allium cepa *** to distilled water was used as a negative control,while exposure to glyphosate was used as a positive *** interaction of extracts(as a post-treatment)with the effects of glyphosate was also *** showed that glyphosate and the extracts of both *** and *** reduced the mitotic index as compared with the negative control(distilled water).Surprisingly,however,both extracts from *** and *** caused a partial reversal of the antiproliferative effect of glyphosate when used as a *** also induced the highest number of cells with chromosomal alterations,which was followed by that of *** ***,the extracts from *** did not show any signifi cant genotoxic ***-treatment of glyphosate-treated samples with distilled water allowed a partial recovery of the genotoxic effect of glyphosate,and some of the Psychotria extracts also did ***,post-treatment of glyphosatetreated samples with *** extracts induced a statistically signifi cant apoptotic *** is concluded that *** extracts show antiproliferative effects and are not genotoxic,while extracts of *** show a less potent antiproliferative effect and may induce chromosomal *** fi nding of a partial reversion of the effects of glyphosate by a post-treatment with extracts from both plants should be followed up.

关键词： medicinal plants Psychotria brachypoda Psychotria birotula cell proliferation apoptosis

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