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Allergenicity assay of allergen from Dermatophagoides farinae in transgenic tobacco

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Chinese Science Bulletin 2004年第5期49卷 452-455页

作者： TANGMingjuan shenye HUYuanlei CAOLei NITing ZHANGHongyu LINZhongping NationalKeyLaboratoryofProteinEngineeringandPlantGeneticEngineering CollegeofLifeSciencesPekingUniversityBeijing100871China DepartmentofAllergy PekingUnionMedicalCollegeHospitalChineseAcademyMedicalScienceBeijing100730China

Derf2 gene for one of mite allergens in Dermatophagoides farinae has been cloned and expressed under regulation of 35S promoter in transgenic tobacco. The tran-scriptional analysis showed that this mite complete gene structure in genomic sequence could be spliced at prediction site. Allergenicity assay with immunological sera indicated that the extracts from the transgenic tobacco gave obvious positive IgE binding reaction with specific serum pool. This work would be of potential use in allergenicity assessment of genetically modified food.

关键词：变应原性表皮螨属昆虫烟草基因转移安全性虫害防治

Hydrogeochemistry and Origin of Thermal Groundwater in Bedrock Aquifers in Tianjin, China

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Journal of China University of Geosciences 2004年第1期15卷 110-114页

作者： ZhouXun FangBin shenye ZhangHua LinLi LinJianwang SchoolofWaterResourcesandEnvironment ChinaUniversityofGeosciencesBeijing100083China TianjinInstituteofGeothermalExplorationDevelopmentandDesign Tianjin300250China

Thermal groundwater resources were found to have occurred in deep-seated bedrock aquifers in the northeastern North China plain near Tianjin, China. Meso- to Neo-Proterozoic and Paleozoic carbonate rocks on the Cangxian uplift are capable of yielding 960-4 200 m 3/d of 60 to 96 ℃ water from the wells ranging in depth between 1 000 and 4 000 m. Conductive heat flow of 0.063 to 0.144 2 W/m 2 from the deep crust is responsible for this anomalous geothermal field. The water in the Ordovician aquifer is characterized by relatively high TDS, high concentrations of SO 4 and SO 4·Cl-Na·Ca type, but the waters from the Meso- to Neo-Proterozoic and Cambrian aquifers, by relatively low TDS, low concentrations of SO 4 and predominantly Cl·SO 4-Na type. It is noted that when the temperature of the waters increases at a rate of 10 ℃ in the range of 30-100 ℃, the content of SiO 2 increases at a rate of 12 to 15 mg/L, and fluoride concentration increases at a rate of 2.3 to 2.5 mg/L. Hydrochemical and isotopic data suggest that the thermal water in the bedrock aquifers is of meteoric origin and recharged in the northern mountain area to the north of the Baodi-Ninghe fault, and then flows laterally for a long distance from the north to the south to the city of Tianjin. Temperature of the waters increases because of heat exchange with the rocks and recharge by conductive heat flow from beneath.

关键词： thermal groundwater hydrogeochemistry isotope geothermal field geothermal resources.

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装饰纸印刷常见问题及解决方法

中国人造板 2024年第9期31卷 12-16页

作者：骆清友刘涛陈皞顾沈业汪广辉江苏佳饰家新材料集团股份有限公司江苏常州213000

深入分析人造板饰面用装饰纸印刷出现的色差、刀线、塞版、局部漏印、水纹、白点、雾点、墨点等缺陷产生的原因,并提出了解决措施,为装饰纸高质量印刷提供参考。

关键词：装饰纸印刷质量

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Experiments and analysis of gold disk targets irradiated by smoothing beams of Xingguang Ⅱ facilities with 350 nm wavelength

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Science China(Physics,Mechanics & Astronomy) 2007年第6期50卷 716-730页

作者： JIANG ShaoEn ZHANG BaoHan LIU shenye YANG JiaMin SUN KeXu HUANG TianXuan DING YongKun ZHENG ZhiJian National Key Laboratory of Laser Fusion Laser Fusion Research CenterMianyang 621900China

Gold disk targets were irradiated using focusing and beam smoothing methods on Xingguang (XG-Ⅱ) laser facilities with 350 nm wavelength,0.6 ns pulse width and 20-80 Joules energies. Laser absorption,light scattering and X-ray conversion were experimentally investigated. The experimental results showed that laser ab-sorption and scattered light were about 90% and 10%,respectively,under focusing irradiation,but the laser absorption increased 5%-10% and the scattered light about 1% under the condition of beam smoothing. Compared with the case of fo-cusing irradiation,the laser absorption was effectively improved and the scattered light remarkably dropped under uniform irradiation; then due to the decrease in laser intensity,X-ray conversion increased. This is highly advantageous to the in-ertial confinement fusion. However,X-ray conversion mechanism basically did not change and X-ray conversion efficiency under beam smoothing and focusing irra-diation was basically the same.

关键词： beam smoothing lens array laser absorption scattered light X-ray conversion

Improvement of Drought Tolerance in Transgenic Tobacco Plants by a Dehydrin-Like Gene Transfer

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Agricultural Sciences in China 2004年第8期3卷 575-583页

作者： shenye JIAWei-long ZHANGYan-qin HUYuan-lei WUQi LINZhongping NationalKeyLaboratoryofProteinEngineeringandPlantGeneEngineering PekingUniversityBeijing100871P.R.China ResearchCenterforDrought ShanxiAcademyofAgricultureSciencesTaiyuan030031P.R.China

A full-length cDNA of dehydrin BcDh2 from Boea crassifolia and its antisense nucleotidesequence have been transferred into tobacco (Nicotiana tabacum) NC89 under the controlof a caulifower mosaic virus 35S promoter. Under a progressive water stress, photosyntheticrate, transpiration rate and stomatal conductance of the sense and antisense plantsreduced, and those of the control reduced much more. Photosynthetic rate, transpirationrate and stomatal conductance of all plants tested increased significantly 24 hourslater after recoveried water supply, and those of the sense and antisense plants werehigher than control. These indicated that overexpression of a dehydrin gene in tobaccomay improve tolerance to water stress for plants, however, antisense BcDh2 gene intransgenic plant did not influence physiological conditions. The results of germinationexperiment of the transgenic seeds showed that on MS medium with different concentrationPEG (8000), sense seed could more endure drought than control, while antisense seed wassensitive to drought. The results suggested that the overexpression of a dehydrin genein tobacco might improve the tolerance to water stress for plants.

关键词： Dehydrin Sense Antisense Drought tolerance

华北区域本底地区夏季近地面臭氧来源的量化解析

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气象与环境学报 2024年第2期40卷 103-107页

作者：刘宁微马思萌杨森权维俊李丽光王若男李裕赫战莘烨上甸子国家大气本底站北京101507 中国气象局沈阳大气环境研究所辽宁沈阳110166 中国民航大学天津300300 北京城市气象研究院北京100089 北京市气象台北京100097 辽宁省气象装备保障中心辽宁沈阳110166 中国民航东北地区空中交通管理局气象中心辽宁沈阳110161 鞍山市气象局辽宁鞍山114004

以北京上甸子国家大气本底站代表华北区域本底地区,利用CAMx模式的臭氧(O_(3))源解析方法(OSAT)对2019年6月25日至7月31日该地区O_(3)浓度进行模拟研究,量化不同来源组分和追踪区域的贡献,分析O_(3)浓度的来源构成。结果表明:对华北区... 详细信息

以北京上甸子国家大气本底站代表华北区域本底地区,利用CAMx模式的臭氧(O_(3))源解析方法(OSAT)对2019年6月25日至7月31日该地区O_(3)浓度进行模拟研究,量化不同来源组分和追踪区域的贡献,分析O_(3)浓度的来源构成。结果表明:对华北区域本底地区地面O_(3)浓度贡献由大到小的组分依次为边界输送(61.8%)、源区输送(31.8%)、背景浓度(3.3%)和本地生成(3.1%),主要追踪源区域依次为河北(5.70%)、内蒙古(5.43%)和山东(4.48%),源类别依次为民用源(15.35%)、农业源(10.72%)、交通源(7.21%)和工业源(1.62%),主要前体物依次为NO_(x)(29.6%)和VOCs(5.3%)。华北区域本底地区地面O_(3)浓度主要由边界输送和源区输送贡献,这进一步验证了上甸子站作为大气本底站的合理性。

关键词：大气本底站臭氧 CAMx 源解析方法

Investigation Into the Electromagnetic Impulses From Long- Pulse Laser Illuminating Solid Targets Inside a Laser Facility

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Photonic Sensors 2016年第3期6卷 249-255页

作者： Tao YI Jinwen YANG Ming YANG Chuanke WANG Weiming YANG Tingshuai LI shenye LIU Shaoen JIANG Yongkun DING Shaoqiu XIAO Laser Fusion Research Center Chinese Academy of Engineering Physics Mianyang 621900 China School of Energy Science and Engineering University of Electronic Science and Technology of China Chengdu611731 China School of Physical Electronics University of Electronic Science and Technology of China Chengdu 611731 China

Emission of the electromagnetic pulses （EMP） due to laser-target interaction in laser facility had been evaluated using a cone antenna in this work. The microwave in frequencies ranging from several hundreds of MHz to 2 GHz was recorded when long-pulse lasers with several thousands of joules illuminated the solid targets, meanwhile the voltage signals from 1V to 4V were captured as functions of laser energy and backlight laser, where the corresponding electric field strengths were obtained by simulating the cone antenna in combination with conducting a mathematical process （Tiknohov Regularization with L curve）. All the typical coupled voltage oscillations displayed multiple peaks and had duration of up to 80ns before decaying into noise and mechanisms of the EMP generation was schematically interpreted in basis of the practical measuring environments. The resultant data were expected to offer basic know-how to achieve inertial confinement fusion.

关键词： Electromagnetic impulse laser voltage simulation decay

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双板电极结构X射线变像管数值模拟

强激光与粒子束 2010年第11期22卷 2593-2595页

作者：陈韬胡盺刘慎业中国工程物理研究院激光聚变研究中心四川绵阳621900 中国工程物理研究院研究生部四川绵阳621900

对条纹相机所使用的一种具有双板电极结构的变像管(Bilamellar)进行了研究,这种变像管使用平板状电极和电四极透镜等设计,改善了传统轴对称结构变像管存在的不足。通过仿真模拟的方法建立了Bilamellar变像管的工作模型,对其结构构成、... 详细信息

对条纹相机所使用的一种具有双板电极结构的变像管(Bilamellar)进行了研究,这种变像管使用平板状电极和电四极透镜等设计,改善了传统轴对称结构变像管存在的不足。通过仿真模拟的方法建立了Bilamellar变像管的工作模型,对其结构构成、工作模式、成像特点等性能作出了分析评估。计算结果表明:根据这一思想设计的变像管结构可以更有效地控制空间电荷效应,并提高时间、空间分辨能力。

关键词：条纹相机电子光学系统模拟分析 Bilamellar结构

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基于时标系统的条纹相机动态畸变

强激光与粒子束 2011年第7期23卷 1718-1720页

作者：邓博曹柱荣陈韬胡昕刘慎业中国工程物理研究院激光聚变研究中心四川绵阳621900

针对神光Ⅲ原型实验中出现的动态畸变现象,开展了条纹相机动态成像特性研究。结合实验图像,利用CST Particle Studio软件模拟了强信号下变像管的成像过程,结果表明:在强信号下,变像管的空间电荷效应会造成电子束聚焦点与理想成像面距离... 详细信息

针对神光Ⅲ原型实验中出现的动态畸变现象,开展了条纹相机动态成像特性研究。结合实验图像,利用CST Particle Studio软件模拟了强信号下变像管的成像过程,结果表明:在强信号下,变像管的空间电荷效应会造成电子束聚焦点与理想成像面距离减小,使得图像的放大倍数变小,产生信号弯曲现象,并且这一现象随着电流强度的增加更为明显。

关键词：动态畸变空间电荷效应变像管条纹相机