Allergenicity assay of allergen from Dermatophagoides farinae in transgenic tobacco

作     者：TANGMingjuan SHENYe HUYuanlei CAOLei NITing ZHANGHongyu LINZhongping 

作者机构：NationalKeyLaboratoryofProteinEngineeringandPlantGeneticEngineeringCollegeofLifeSciencesPekingUniversityBeijing100871China DepartmentofAllergyPekingUnionMedicalCollegeHospitalChineseAcademyMedicalScienceBeijing100730China 

出 版 物：《Chinese Science Bulletin》 (中国科学通报)

年 卷 期：2004年第49卷第5期

页      面：452-455页

核心收录：

学科分类：09[农学] 0904[农学-植物保护] 090401[农学-植物病理学] 090402[农学-农业昆虫与害虫防治] 

基　　金：2001CB10900 

主　　题：变应原性 表皮螨属昆虫 烟草 基因转移 安全性 虫害防治 

摘      要：Derf2 gene for one of mite allergens in Dermatophagoides farinae has been cloned and expressed under regulation of 35S promoter in transgenic tobacco. The tran-scriptional analysis showed that this mite complete gene structure in genomic sequence could be spliced at prediction site. Allergenicity assay with immunological sera indicated that the extracts from the transgenic tobacco gave obvious positive IgE binding reaction with specific serum pool. This work would be of potential use in allergenicity assessment of genetically modified food.