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《内科学》教学“痛点”及应对策略

中国继续医学教育 2024年第14期16卷 8-13页

作者：杜国利魏伊函姚雪燕蒋升新疆医科大学第一附属医院内分泌科新疆乌鲁木齐830000

为了培养不断提升自身修养的优秀临床医师,文章探讨了《内科学》教学创新过程中的教学“痛点”及应对策略。文章旨在坚持“以学生为中心”的教学理念,根据学生学习过程中面临的实际问题,探寻痛点,结合教学创新,寻找应对策略。《内科学... 详细信息

为了培养不断提升自身修养的优秀临床医师,文章探讨了《内科学》教学创新过程中的教学“痛点”及应对策略。文章旨在坚持“以学生为中心”的教学理念,根据学生学习过程中面临的实际问题,探寻痛点,结合教学创新,寻找应对策略。《内科学》在高等医学教育教学过程中发现四大“痛点”,即重外轻内、浅尝辄止、难遇罕见、学不及新。针对以上痛点,创新性地提出并践行“德以明理,笃志好学”的创新教育理念,分析对策,戳痛点、促改革。“德以明理”着重解决“重外轻内”过程中的思想问题。“笃志好学”理念着眼于解决“浅尝辄止”“难遇罕见”“学不及新”三大学习痛点。经过“戳痛点、促改革”的教学创新实践,《内科学》教学质量与教学效果显著提升。

关键词：痛点改革策略内科学教学教学创新大赛以学生为中心教学

高脂饮食对小鼠胰岛功能及胰腺组织中TCF7L2/GLP-1的影响

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山西医科大学学报 2024年第7期55卷 872-878页

作者：孟齐艾比拜·玉素甫刘华蒋升省部共建中亚高发病成因与防治国家重点实验室新疆医科大学第一附属医院内分泌科乌鲁木齐830000

目的探讨高脂饮食对小鼠胰岛功能及胰腺组织中转录因子7-类似物2(TCF7L2)与胰高血糖素样肽-1(GLP-1)的影响。方法30只小鼠随机均分为2组:正常对照组和高脂饮食(T2DM模型)组,分别连续24周喂养普通饲料和60%高脂饲料。连续喂养24周后比较... 详细信息

目的探讨高脂饮食对小鼠胰岛功能及胰腺组织中转录因子7-类似物2(TCF7L2)与胰高血糖素样肽-1(GLP-1)的影响。方法30只小鼠随机均分为2组:正常对照组和高脂饮食(T2DM模型)组,分别连续24周喂养普通饲料和60%高脂饲料。连续喂养24周后比较两组小鼠的体质量以及胰岛功能等相关指标的差异;检测小鼠不同时间点的血糖水平,血浆中胰岛素、高密度脂蛋白(HDL-C)、低密度脂蛋白(LDL-C)、总胆固醇(TC)和甘油三酯(TG)的水平,胰腺组织中TCF7L2、GLP-1和胰高血糖素样肽-1受体(GLP-1R)蛋白的表达水平和mRNA转录水平。结果与正常对照组比较,高脂饮食组小鼠的体质量显著增加(P<0.01),葡萄糖耐量和胰岛素耐受能力明显受损(P<0.05),HDL-C、LDL-C、TC、TG、空腹血糖和胰岛素的水平明显增加(P<0.05),而TCF7L2、GLP-1和GLP-1R的mRNA和蛋白表达水平明显降低(P<0.01)。另外,与正常对照组相比,高脂饮食组小鼠胰腺组织的胰岛形态不规则,体积增大,血管数量增多,扩张充血显著。结论高脂饮食的T2DM小鼠,胰腺组织胰岛形态改变,胰岛功能降低,TCF7L2表达水平降低,并且GLP-1和GLP-1R表达水平同步降低。

关键词： 2型糖尿病转录因子7类似物2 胰高血糖素样肽-1 胰腺胰岛素

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Expression of Dickkopf-related Protein 1 in Patients with Temporomandibular Osteoarthritis after Treatment with Hyaluronic Acid

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Current Medical Science 2020年第3期40卷 574-579页

作者： sheng-jun jiang Zhong-zhi JIN You-jian PENG Department of Stomatology Renmin Hospital of Wuhan UniversityWuhan430060China

We previously reported that the increased expression of Dickkopf-related protein 1(DKK1)is positively related to vascular endothelial growth factor in the synovial fluid from patients with temporomandibular joint disorders(TMDs).DKK1 is involved in angiogenic activities in the TMD synovium in vitro,but the expression of DKK1 after treatment of TMD-osteoarthritis(TMD-OA)with hyaluronic acid(HA)remains *** this study,we assessed the expression of DKK1 in the synovial fluid of TMD-OA patients before and after treatment with HA via enzyme-linked immunosorbent *** also investigated the role of DKK1 in TMD-OA via immunohistochemical *** relationship between the expression of DKK1 and the clinicopathological characteristics was determined by Pearson *** results showed that the expression of DKK1 was significantly decreased after treatment with *** analyses indicated that the expression of DKK1 in the TMD-OA samples was closely correlated with mouth opening and *** findings suggest that DKK1 could play an important role in the pathogenesis and treatment of *** of the pain by HA treatment may be correlated with the decreased expression of DKK1.

关键词： Dickkopf-related protein 1 temporomandibular joint disorders hyaluronic acid synovial fluid

Effects of different ingredients of zedoary on gene expression of HSC-T6 cells

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World Journal of Gastroenterology 2005年第43期11卷 6780-6786页

作者： Yuan jiang Ze-Song Li Fu-sheng jiang Xin Deng Cong-Shun Yao Guang Nie Shenzhen Shajing People's Hospital Shenzhen 518104 Guangdong Province China Shenzhen Yishengtang Biology Enterprise Company Shenzhen 518126 Guangdong Province China Shenzhen Donghu Hospital Shenzhen 518020 Guangdong Province China Teaching pharmacology and Research Section of Shenyang Pharmaceutial University Shenyang 110000 Liaoning Province China

AIM： To investigate the effects of four different ingredients of zedoary （Curcuma aromatica oil, Curcumol, β-elemence, and Curcumin） on the gene expressions of hepatic stellate cells （HSCs）, and to explore the molecular mechanism of zedoary against hepatic fibrosis at gene network level. METHODS： We detected the mRNA sequences of 50 liver fibrosis-related genes in GenBank and designed oligonucleotide probes. We synthesized oligonucleotides with PE8909 DNA synthesizing instrument, and carried out oligonucleotide microarray with OGR-04 dropping instrument and aldehyded glass chip. Cultured HSC-T6 cells were breated wibh different concentrations of Colchicine, Curcuma aromatica oil, Curcumol, β-elemence, and Curcumin. According to the experiment of cell toxicity, we took the appropriate concentrations of medicines that resulted in over 50% of cell survival as experiment concentrations. We collected the cells at 1, 6, 12, and 24 h, and extracted total RNA with TRIzol reagent, then labeled cDNAs with Cy3-dUTP and Cy5-dUTP. These labeled cDNAs were hybridized to an oligonucleotide microarray which was washed several times and scanned by scanner GenePix 4000B. Different gene expressions of HSC-T6 cells were analyzed by ImaGene 4.2 software. RESULTS： After HSC-T6 cells were cultured in a medium containing 6.25μg/mL Colchicine for 12 h, expression of TIMP-1 decreased 2.2-folds. After HSC-T6 cells were cultured in a medium containing 78.125 μg/mL of Curcuma aromatica oil for 24 h, the expression of TIMP-2 and IL-6 decreased 2.3- and 2.2-folds, respectively. Moreover, after HSC-T6 cells were cultured in a medium containing 1.5625 μg/mL of Curcumol for 12 h, the expression of TGFμ1 and P450a decreased 2.3- and 2.1-folds, respectively. CONCLUSION： Our results may show the possible molecular mechanism of Curcuma aromatica oil and Curcumol against hepatic fibrosis.

关键词： DNA microarray Curcuma aromatica oil Curcumol Hepatic stellate cells Hepatic fibrosis

SOX2-dependent expression of dihydroorotate dehydrogenase regulates oral squamous cell carcinoma cell proliferation

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International Journal of Oral Science 2021年第1期13卷 62-70页

作者： Xuemei Qiu sheng jiang Yanxuan Xiao Yumin He Tao Ren Lu jiang Rui Liu Qianming Chen State Key Laboratory of Oral Diseases&National Clinical Research Center for Oral Diseases&Chinese Academy of Medical Sciences Research Unit of Oral Carcinogenesis and Management West China Hospital of StomatologySichuan UniversityChengduChina Ministry of science and technology The Second Affiliated Hospital of Chengdu Medical College(China National Nuclear Corporation 416 Hospital)ChengduChina Oncology Department Clinical Medical College and The First Affiliated Hospital of Chengdu Medical CollegeChengduChina

Oral squamous cell carcinoma(OSCC)become a heavy burden of public health,with approximately 300000 newly diagnosed cases and 145000 deaths worldwide per *** metabolism fuel DNA replication and RNA synthesis,which is indispensable for cell *** how tumor cells orchestrate nucleotide metabolic enzymes to support their rapid growth is largely *** we show that expression of pyrimidine metabolic enzyme dihydroorotate dehydrogenase(DHODH)is upregulated in OSCC tissues,compared to non-cancerous adjacent *** expression of DHODH is correlated with a shortened patient survival *** of DHODH by either shRNA or selective inhibitors impairs proliferation of OSCC cells and growth of tumor ***,loss of functional DHODH imped de novo pyrimidine synthesis,and disrupt mitochondrial respiration probably through destabilizing the MICOS *** study shows that transcriptional factor SOX2 plays an important role in the upregulation of DHODH in *** findings add to the knowledge of how cancer cells co-opt nucleotide metabolism to support their rapid growth,and thereby highlight DHODH as a potential prognostic and therapeutic target for OSCC treatment.

关键词： SOX2 metabolism squamous

高应力炭质板岩隧道开挖支护结构受力评价研究

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科学技术与工程 2024年第4期24卷 1689-1698页

作者：胡涛涛高咸超王青松谢江胜涂鹏长安大学公路学院西安710064 中铁二十局集团有限公司西安710016 中国铁建高原隧道施工技术及装备研发中心西安710016

为探究高应力下炭质板岩隧道开挖过程中围岩位移、支护结构内力变化规律,依托渭武高速木寨岭公路隧道,采用有限差分软件FLAC3D建立三台阶七步开挖法下炭质板岩隧道的数值模型,提出强、中、弱3种支护方案;分析开挖距离对围岩位移、支护... 详细信息

为探究高应力下炭质板岩隧道开挖过程中围岩位移、支护结构内力变化规律,依托渭武高速木寨岭公路隧道,采用有限差分软件FLAC3D建立三台阶七步开挖法下炭质板岩隧道的数值模型,提出强、中、弱3种支护方案;分析开挖距离对围岩位移、支护结构内力等因素的影响,并对支护设计参数合理性评价。研究结果表明:开挖过程中围岩累计沉降和收敛变形量比较:强支护<中支护<弱支护;开挖相同距离下,随着支护强度上升,支护提供的弹性抗力越强,围岩沉降和收敛变形减小;以强支护为例,支护结构轴力呈现先增大后减小的趋势,最终轴力不断上升但趋势变缓的特征,开挖距离50 m趋于稳定;支护设计参数评价时,开挖距离(50 m)结束,强、中、弱3种方案钢架受压安全系数最小值分别为3.903、3.718、3.264,#1拱顶处强支护钢架较喷射混凝土先破坏,混凝土安全系数最小值分别为3.491、2.987、2.666,#2左拱腰处喷射混凝土较钢架先破坏,故选择中支护方案,材料I25b×C25、初衬厚度26 cm、钢架距离0.8 m。研究成果可为类似软岩隧道开挖和支护结构的设计提供一定参考。

关键词：炭质板岩围岩位移支护结构内力数值模拟合理性评价

Ginkgolide B promotes the proliferation and differentiation of neural stem cells following cerebral ischemia/reperfusion injury,both in vivo and in vitro

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Neural Regeneration Research 2018年第7期13卷 1204-1211页

作者： Pei-Dong Zheng Rajneesh Mungur Heng-Jun Zhou Muhammad Hassan sheng-Nan jiang Jie-sheng Zheng Department of Neurosurgery the First Affiliated HospitalCollege of MedicineZhejiang University

Neural stem cells have great potential for the development of novel therapies for nervous system ***,the proliferation of endogenous neural stem cells following brain ischemia is insufficient for central nervous system *** B has a robust neuroprotective *** this study,we investigated the cell and molecular mechanisms underlying the neuroprotective effect of ginkgolide B on focal cerebral ischemia/reperfusion injury in vitro and in *** stem cells were treated with 20,40 and 60 mg/L ginkgolide B in *** staining was used to assess cellular expression of neuron-specific enolase,glial fibrillary acid protein and suppressor of cytokine signaling *** treatment with 40 and 60 mg/L ginkgolide B,cells were large,with long ***,the proportions of neuron-specific enolase-,glial fibrillary acid protein-and suppressor of cytokine signaling 2-positive cells increased.A rat model of cerebral ischemia/reperfusion injury was established by middle cerebral artery *** hours after ischemia,ginkgolide B（20 mg/kg） was intraperitoneally injected,once a *** Longa＇s method was used to assess neurological *** was performed to evaluate the proportion of nestin-,neuron-specific enolase-and glial fibrillary acid protein-positive ***-time quantitative polymerase chain reaction was used to measure m RNA expression of brain-derived neurotrophic factor and epidermal growth *** blot assay was used to analyze the expression levels of brain-derived neurotrophic factor and suppressor of cytokine signaling *** B decreased the neurological deficit score,increased the proportion of nestin-,neuron-specific enolase-and glial fibrillary acid protein-positive cells,increased the m RNA expression of brain-derived neurotrophic factor and epidermal growth factor,and increased the expression levels of brain-derived neurotrophic factor and suppressor of cytokine sig

关键词： nerve regeneration brain-derived neurotrophic factor epidermal growth factor suppressor of cytokine signaling 2 neuron-specific enolase glial fibrillary acid protein nestin bromodeoxyuridine neurological function middle cerebral artery occlusion astrocytes neural regeneration

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沥青路面压实过程的数字化控制指标

公路 2024年第3期69卷 1-5页

作者：蒋盛川董晨阳林雨超翁梓航向晖新一代人工智能技术应用交通运输行业研发中心杭州市310023 上海理工大学交通工程系上海市200093 中建铁路投资建设集团有限公司北京市102600 同济大学道路与交通工程教育部重点实验室上海市201800

传统的沥青路面压实质量检测手段无法实现实时监测与控制,然而三维扫描技术的引入为压实过程的数字化控制提供了可能。利用车辙试样成型机在实验室内对不同级配的车辙板进行压实,并分析沥青路面压实过程中的最大峰高(Sp)和平均断面深度(... 详细信息

传统的沥青路面压实质量检测手段无法实现实时监测与控制,然而三维扫描技术的引入为压实过程的数字化控制提供了可能。利用车辙试样成型机在实验室内对不同级配的车辙板进行压实,并分析沥青路面压实过程中的最大峰高(Sp)和平均断面深度(MPD)两个三维纹理特征参数的变化规律。研究结果显示,MPD数值持续减少,而Sp在压实初期迅速下降,后期趋于收敛。为验证Sp作为控制压实度的指标的有效性,采用Bland-Altman模型对Sp的一致性进行了分析,结果显示Sp与压实度之间具有良好的一致性。基于数字化的数据采集手段和计算方法,提出了沥青路面压实质量评价和控制的数字化指标,为沥青路面施工的数字化和实时控制提供了新思路。

关键词：沥青路面压实过程三维纹理特征压实控制指标

Tribocorrosion Behavior of 304 Stainless Steel in 0.5 mol/L Sulfuric Acid

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Acta Metallurgica Sinica(English Letters) 2018年第10期31卷 1049-1058页

作者： Ming Liu De-Li Duan sheng-Li jiang Ming-Yang Li Shu Li Institute of Metal Research Chinese Academy of Sciences School of Materials Science and Engineering University of Science and Technology of China

The tribocorrosion behavior of 304 stainless steel was studied through comparing the damage behavior of 304 stainless steel in dilute sulfuric acid to that in distilled water by a reciprocating tribotester. The re-passivation behavior, the surface and sectional morphological features, as well as the change of microhardness of samples were studied, and the tribocorrosion mechanism was also discussed. The experimental results reveal that the damage of stainless steel in dilute sulfuric acid was caused by the steel＇s mechanical removal and electrochemical dissolution. The wear mechanism of stainless steel is abrasive wear, which mainly consists of micro-cutting and peeling. The synergetic action between corrosion and wear is notable. The corrosive environment leads to the embrittlement of the surface layer, and the wear destroys the passivation film and causes galvanic corrosion.

关键词： Stainless steel Tribocorrosion Synergetic action Reciprocating wear test Re-passivation