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基于Lamb波的压电陶瓷/环氧树脂复合材料传感器制备及应用

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仪器仪表学报 2019年第12期40卷 19-25页

作者：沈意平刘缘王钢韩斌蒋帅湖南科技大学机械设备健康维护湖南省重点实验室湘潭411201 华中科技大学数字制造装备与技术国家重点实验室武汉430074 湖南铁道职业技术学院株洲412001

风力机叶片、飞机机翼等结构均为大面积曲面板类结构,基于Lamb波的结构健康检测技术对细微损伤比较敏感,是目前最具应用前景的技术之一。传统的Lamb波传感器大都采用PZT陶瓷制成,质地脆且硬度大,不能适应于曲面结构检测。将PZT陶瓷粉末... 详细信息

风力机叶片、飞机机翼等结构均为大面积曲面板类结构,基于Lamb波的结构健康检测技术对细微损伤比较敏感,是目前最具应用前景的技术之一。传统的Lamb波传感器大都采用PZT陶瓷制成,质地脆且硬度大,不能适应于曲面结构检测。将PZT陶瓷粉末与环氧树脂复合制备了一种新型柔性0-3型压电复合材料,研究了质量比、极化电场、极化温度和极化时间等因素对压电复合材料性能参数的影响,开展了多因素正交实验来确定材料制备的最优极化工艺参数。实验研究了该压电复合材料制成的传感器对Lamb波的传感响应特性,与现有的MFC、PVDF和传统压电片等传感元件的响应特性进行了对比分析。将制备的传感器应用于翼型曲面板,利用椭圆定位方法进行损伤检测。研究结果表明,PZT陶瓷/环氧树脂压电复合材料传感器具有良好的传感响应特性,能够很好地贴合于曲面板表面,与只能部分耦合于曲面板的传统压电片相比,采集的Lamb波信号更加准确,从而为曲面板类结构健康监测提供一种新型的柔性压电传感技术。

关键词：压电复合材料曲面结构正交实验柔性传感器传感响应特性

X-linked adrenal hypoplasia congenita and hypogonadotropic hypogonadism:identification and in vitro study of a novel small indel in the NR0B1 gene

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X-linked adrenal hypoplasia congenita and hypogonadotropic h...

Causal variants screened by whole exome sequencing in a patient with maternal uniparental disomy of chromosome 10 and a complicated phenotype

第十四次全国医学遗传学学术会议

作者： Tingting Yu Jian Wang Yongguo Yu Xiaodong Huang yiping shen Institute for Pediatric Translational Medicine Shanghai Children' s Medical CenterShanghai Jiaotong University School of Medicine Department of Pediatric Endocrinology/Genetics Xinhua HospitalShanghai Jiao Tong University School of Medicine Department of Internal Medicine Shanghai Children' s Medical CenterShanghai Jiaotong University School of Medicine Department of Medical Genetics Shanghai Children' s Medical CenterShanghai Jiaotong University School of Medicine Department of Laboratory Medicine Boston Children's Hospital

DAX1 is an orphan nuclear receptor that plays a key role in the development and function of the adrenal and reproductive *** in the gene encoding DAX1(NR0B1)result in X-linked adrenal hypoplasia congenita(AHC)and hypogonadotropic hypogonadism(HHG).A Chinese pedigree with X-linked AHC and HHG was investigated in the present *** analysis identified a novel small indel variant,c.19507delinsTG,in the NR0B1 *** determine the effect of this variant on DAX1 expression,quantitative reverse transcription PCR(RT-qPCR)and western blot assays were *** mRNA expression level of the mutant NR0B1 was significantly reduced(62%decrease)compared to that of the wild type(WT).Westernblot assay indicated that the c.19507delinsTG mutation could lead to various truncated DAX1 proteins,including the C-terminal truncated DAX1,which was only detected in the cytoplasm,and the N-terminal truncated DAX1,which was distributed in both the cytoplasm and *** luciferase assay was employed to monitor the repressor function of DAX1 in modulating SF-1 mediated ***1 WT significantly suppressed the SF-1 mediated StAR promoter activity by 35.5±1.9%.In contrast to other known pathogenic mutations which abolish the repressor function of DAX1,the c.19507delinsTG mutant showed a stronger repressor activity,demonstrating a 49.9±2.6%reduction of promotor *** findings suggest that the NR0B1 mutational mechanism for X-linked AHC and HHG is not uniformly loss of function or DAX1 is likely to be involved with molecular functions other than its role as a repressor of SF-1 activation.

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Causal variants screened by whole exome sequencing in a pati...

HPDL deficiency causes a neuromuscular disease by impairing the mitochondrial respiration

第十四次全国医学遗传学学术会议

作者： Niu Li Yu Ding Juan Li Yongnian shen yiping shen Xiaodong Huang Jian Wang Institute of Pediatric Translational Medicine Shanghai Children' s Medical CenterShanghai Jiaotong University School of Medicine Department of Endocrinology and Metabolism Shanghai Children' s Medical CenterShanghai Jiaotong University School of Medicine Department of Laboratory Medicine Shanghai Children' s Medical CenterShanghai Jiaotong University School of Medicine

Background:Uniparental disomy(UPD)is to describe the abnormal situation that both copies of one chromosome pair are inherited from only one *** lead to clinical abnormalities through affecting genomic imprinting or resulting in autosomalrecessive *** exome sequencing and chromosomal microarrays are two powerful technologies to search for underlying causal ***:Whole exome sequencing was used to screen for candidate causal variants in the genome of a Chinese pediatric patient,who was found to have maternal uniparental isodisomy of chromosome10 detected by chromosomal microarrays with severe multiple medical problems,including bilateral deafness,binocular blindness,growth failure and ***:Thirteen rare homozygous variants on chromosome 10 were identified,of these genes,a classical splice variant in HPS1 gene(c.398+5G>A)resulting in Hermansky-Pudlak syndrome type 1 was a candidate to explain the patient’s ocular and dermal ***,seven probably pathogenic genes linked to the subject’s ocular andaural disorders were detected by phenotypic analysis strategy,and several candidate genes may contribute to the subject’s complicated phenotype,including MY01A gene and NR2E3 ***:We successfully combine whole exome sequencing and chromosomal microarrays these technologies to identify several candidate causal genes in a Chinese pediatric patient with severe multiple medical problems,to a certain extent,we establish the connection of phenotypic and genotypic in the patient.

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Journal of Genetics and Genomics 2021年第8期48卷 727-736页

作者： Yu Sun Xiujuan Wei Fang Fang yiping shen Haiyan Wei Jiuwei Li Xianglai Ye Yongkun Zhan Xiantao Ye Xiaomin Liu Wei Yang Yuhua Li Xiangju Geng Xuelin Huang Yiyan Ruan Zailong Qin Shang Yi Jianxin Lyu Hezhi Fang Yongguo Yu Department of Pediatric Endocrinology and Genetics Xinhua HospitalShanghai Jiao Tong University School of MedicineShanghai Institute for Pediatric ResearchShanghai 200092China Key Laboratory of Laboratory Medicine Ministry of EducationZhejiang Provincial Key Laboratory of Medical GeneticsSchool of Laboratory Medicine and Life SciencesWenzhou Medical UniversityWenzhou 325035China Department of Neurology Beijing Children's HospitalCapital Medical UniversityNational Center for Children's HealthBeijing 100045China The Maternal and Child Health Care Hospital of Guangxi Zhuang Autonomous Region Guangxi Birth Defects Prevention and Control InstituteNanning 530000China Shanghai Children's Medical Center Shanghai Jiao Tong University School of MedicineShanghai 200127China Boston Children's Hospital Harvard Medical SchoolBostonMA 02115USA Department of Endocrinologic and Inherited Metabolic Henan Childen's HospitalZhengzhou Children's HospitalZhengzhou 450078China Department of Radiology Xinhua HospitalShanghai Jiao Tong University School of MedicineShanghai 200092China Department of Rehabilitation Henan Children's HospitalZhengzhou Children's HospitalZhengzhou 450018China Zhejiang Provincial People's Hospital Affiliated People's Hospital of Hangzhou Medical CollegeHangzhou 310014China Shanghai Key Laboratory of Pediatric Gastroenterology and Nutrition Shanghai 200092China

Mitochondrial diseases are caused by variants in both mitochondrial and nuclear genomes.A nuclear gene HPDL(4-hydroxyphenylpyruvate dioxygenase-like),which encodes an intermembrane mitochondrial protein,has been recently implicated in causing a neurodegenerative disease characterized by pediatric-onset spastic movement ***,we report six Chinese patients with bi-allelic HPDL pathogenic variants from four unrelated families showing neuropathic symptoms of variable severity,including developmental delay/intellectual disability,spasm,and *** different pathogenic variants are identified,of which five are *** fibroblasts and immortalized lymphocytes derived from patients show impaired mitochondrial respiratory function,which is also observed in HPDL-knockdown(KD)He La *** these He La cells,overexpression of a wild-type HPDL gene can rescue the respiratory phenotype of oxygen consumption *** addition,a decreased activity of the oxidative phosphorylation(OXPHOS)complex II is observed in patient-derived lymphocytes and HPDL-KD He La cells,further supporting an essential role of HPDL in the mitochondrial respiratory ***,our data expand the clinical and mutational spectra of this mitochondrial neuropathy and further delineate the possible disease mechanism involving the impairment of the OXPHOS complex II activity due to the bi-allelic inactivations of HPDL.

关键词： HPDL gene Mitochondrial disease Respiration impairment OXPHOS Respiration chain complexⅡ

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炽盛光佛信仰与宋元社会

世界宗教文化 2019年第1期 83-90页

作者：夏广兴申一平上海师范大学文学院

宋元之际,炽盛光佛信仰是社会上有着广泛影响的密教信仰之一,人们大量雕造或图绘炽盛光佛像及刻印其经咒,以消灾祈福。经言:当天象发生灾异时,可以炽盛光佛为本尊,设立曼荼罗坛场,持诵密咒以修法,可消灾纳福,因此,深得朝野上下广大信众... 详细信息

宋元之际,炽盛光佛信仰是社会上有着广泛影响的密教信仰之一,人们大量雕造或图绘炽盛光佛像及刻印其经咒,以消灾祈福。经言:当天象发生灾异时,可以炽盛光佛为本尊,设立曼荼罗坛场,持诵密咒以修法,可消灾纳福,因此,深得朝野上下广大信众的推崇。本文即以此为视角,引经据典,进一步说明华梵交融,对当时信众精神生活的深层影响。

关键词：密教炽盛光佛星曜经咒

遗传病高通量测序数据分析及变异解读:问题与挑战

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中华医学杂志 2019年第43期99卷 3372-3377页

作者：张括沈亦平李金明北京医院北京老年医学研究中心国家卫生健康委临床检验中心北京100730 广西壮族自治区妇幼保健院遗传代谢中心实验室南宁530000

遗传病发病率低,病种多,诊断困难.遗传病的精准基因诊断是患者及家庭疾病管理和治疗的基础.高通量测序技术的发展,大大提升遗传病患者获得基因诊断的预期,但如何建立可靠的下一代测序技术(NGS)检测方法,以及如何对测序数据进行分析和解... 详细信息

遗传病发病率低,病种多,诊断困难.遗传病的精准基因诊断是患者及家庭疾病管理和治疗的基础.高通量测序技术的发展,大大提升遗传病患者获得基因诊断的预期,但如何建立可靠的下一代测序技术(NGS)检测方法,以及如何对测序数据进行分析和解读,是国内遗传病基因检测需解决的关键问题之一.本文从遗传病NGS数据分析流程的建立与确认、所检测基因的临床有效性和序列变异分类与解读三个方面分析和阐述我国从事遗传病NGS检测的实验室在临床数据分析与解读方面可能存在的问题及面临的挑战.

关键词：遗传病高通量测序技术数据分析变异解读

钢轨裂纹导波检测的柔性压电复合材料传感技术研究

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仪器仪表学报 2021年第11期42卷 62-70页

作者：沈意平吴迪张博南毕超蒋帅湖南科技大学机械设备健康维护湖南省重点实验室湘潭411201 北京信泰智合科技发展有限公司北京100098 中国铁道科学研究院集团有限公司金属及化学研究所北京100081

钢轨裂纹监测是保障铁路基础部件安全的重要研究课题之一。超声导波在钢轨中具有衰减小、传感距离远、检测效率高等优点,可实现钢轨长距离快速检测。传统的超声导波传感器大都采用PZT陶瓷制成,质地硬脆、易碎裂,不能满足钢轨长期在线监... 详细信息

钢轨裂纹监测是保障铁路基础部件安全的重要研究课题之一。超声导波在钢轨中具有衰减小、传感距离远、检测效率高等优点,可实现钢轨长距离快速检测。传统的超声导波传感器大都采用PZT陶瓷制成,质地硬脆、易碎裂,不能满足钢轨长期在线监测使用要求。本文提出将柔性0~3型PZT/环氧树脂复合材料用作钢轨超声导波传感器,实验研究该柔性压电复合材料的力学性能及其对导波的传感特性,结合有限元仿真分析和实验测试,分析了钢轨中激励导波模态特性及其裂纹反射波的延迟到达时间,探讨压电复合材料传感器应用钢轨裂纹导波信号检测的适用性和有效性。研究结果表明:PZT/环氧树脂复合材料传感器具有良好的柔韧性和线性灵敏度,在5℃~75℃温度范围内能够有效检测到导波信号;不同传感路径得到的钢轨导波检测信号与有限元仿真信号基本一致,裂纹反射波到达时间相近,这将为钢轨裂纹在线监测提供新型的柔性压电复合材料传感技术。

关键词： PZT/环氧树脂复合材料超声导波钢轨裂纹到达时间

基于动力学有限元模型的多跨转子轴系无试重整机动平衡研究

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机械工程学报 2016年第21期52卷 78-86页

作者：宾光富李学军沈意平高金吉湖南科技大学机械设备健康维护湖南省重点实验室湘潭411201 北京化工大学诊断与自愈工程研究中心北京100029

针对多跨转子轴系现场动平衡需频繁启停机的问题,结合影响系数平衡法和模态振型平衡法特点,提出一种新的基于动力学有限元模型的轴系无试重整机动平衡法。综合转子动力学相关理论和有限元技术,通过仿真构建与轴系结构尺寸和运行参数相... 详细信息

针对多跨转子轴系现场动平衡需频繁启停机的问题,结合影响系数平衡法和模态振型平衡法特点,提出一种新的基于动力学有限元模型的轴系无试重整机动平衡法。综合转子动力学相关理论和有限元技术,通过仿真构建与轴系结构尺寸和运行参数相符的转子动力学有限元模型。采用柔性转子共振分离原理,分析轴系振型和不平衡振动阶次以确定各跨转子加重平衡平面数和位置,分别在各跨转子平衡位置处施加不平衡激励,获取轴系平衡转速下各振动测点处不平衡响应,计算出相应的加重影响系数,从而取代轴系现场动平衡中需要多次启停机试重测取过程。然后采用最小二乘法通过解平衡矢量方程组得到轴系所需的平衡配重。最后以模拟百万兆瓦超超临界汽轮机的四跨五支承柔性转子轴系试验台为例,应用该方法开展了2700 r/min转速下轴系四个平面同时配重动平衡试验,单点降幅最高达53%,实现了无试重下柔性转子轴系整机动平衡,可有效减少因盲目试重次数,节省平衡费用和周期。

关键词：多跨转子轴系动力学有限元模型加重影响系数无试重整机动平衡

基于压电纤维传感器应力波方向检测的结构冲击定位研究

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仪器仪表学报 2023年第1期44卷 27-37页

作者：蒋帅沈意平王送来支余林宾光富湖南科技大学机械设备健康维护湖南省重点实验室湘潭411201 湖南铁道职业技术学院智能制造学院株洲412001 株洲国创轨道科技有限公司湖南省轨道交通装备智能感知与运维工程技术研究中心株洲412001

冲击定位监测是飞行器结构健康监测的重要研究课题。基于应力波方向检测的冲击定位方法可有效克服传统的到达时间方法依赖波速的应用局限性,适合飞行器复杂结构的冲击定位监测。本文分析了压电纤维对冲击应力波的响应特性,针对板结构冲... 详细信息

冲击定位监测是飞行器结构健康监测的重要研究课题。基于应力波方向检测的冲击定位方法可有效克服传统的到达时间方法依赖波速的应用局限性,适合飞行器复杂结构的冲击定位监测。本文分析了压电纤维对冲击应力波的响应特性,针对板结构冲击应力波信号具有宽频、频散与多模态成分混叠等特征,采用Shannon复数小波变换提取应力波能量峰值窄带信号,通过仿真与实验研究表明低速冲击板中产生的应力波主能量成分为A_(0)模态Lamb波;基于压电纤维对Lamb波的方向响应特性,优化制备了45°“应变花”结构的压电纤维传感器,以能量峰值窄带Lamb波传播方向表征应力波传播方向,提出了基于压电纤维传感器应力波方向检测的冲击定位方法。实验结果表明提出的基于压电纤维传感器应力波方向检测的冲击定位方法准确有效,平均距离定位误差为17.2 mm。研究成果提供一种应力波方向传感器及不依赖应力波波速的结构冲击定位方法,具有一定的理论研究意义和工程应用价值。

关键词：压电纤维应力波方向感知冲击定位窄带信号提取