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In situ synthesis of red fluorescent gold nanoclusters with enzyme-like activity for oxidative stress amplification in chemodynamic therapy

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Chinese Chemical Letters 2022年第3期33卷 1331-1336页

作者： Wenying Mi Shuang Tang shaoshi Guo Hejing Li na shao College of Chemistry Beijing Normal UniversityBeijing 100875China

Chemodynamic therapy (CDT) has attracted tremendous interest in cancer therapy because it is independent of oxygen and photoirradiation. However, the therapeutic efficacy of CDT is restricted by insufficient H_(2)O_(2) levels in tumor cells. Herein, employing endogenous GSH as a template and cationic polymeric chitosan (CS) as crosslinker and stabilizer exhibiting easy cell uptake, red luminescent gold nanoclusters (denoted CS-GSH@AuNCs) were successfully synthesized in HeLa cells. The in situ synthesized CS-GSH@AuNCs exhibited both superoxidase dismutase (SOD) and peroxidase (POD)-like activity, which could promote the production of H_(2)O_(2) from superoxide anion radicals (O_(2)^(·-)) and then ^(·)OH. The combination of GSH elimination and H_(2)O_(2) elevation boosted the generation of ^(·)OH, which could trigger cancer cell apoptosis and death. The enzyme-like activity of CS-GSH@AuNCs could be effectively activated under acidic conditions, and showed a high killing effect on tumor cells but minimal toxicity to normal cells. The developed GSH consumption and ^(·)OH promotion theranostic platform is an innovative route for enhanced CDT by the amplification of oxidative stress.

关键词： Chemodynamic therapy In situ synthesis Gold nanoclusters Superoxidase dismutase and peroxidase(POD)-like activity Oxidative stress amplification

ROS-mediated BNIP3-dependent mitophagy promotes coelomocyte survival in Apostichopus japonicus in response to Vibrio splendidus infection

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Zoological Research 2022年第2期43卷 285-300页

作者： Lian-Lian Sun Yi-na shao Mei-Xiang You Cheng-Hua Li State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products Ningbo UniversityNingboZhejiang 315211China Laboratory for Marine Fisheries Science and Food Production Processes Qingdao National Laboratory for Marine Science and TechnologyQingdaoShandong 266071China State-Province Joint Laboratory of Marine Biotechnology and Engineering Ningbo UniversityNingboZhejiang 315211China

Organisms produce high levels of reactive oxygen species(ROS)to kill pathogens or act as signaling molecules to induce immune responses;however,excessive ROS can result in cell *** maintain ROS balance and cell survival,mitophagy selectively eliminates damaged mitochondria via mitophagy receptors in *** marine invertebrates,however,mitophagy and its functions remain largely *** the current study,Vibrio splendidus infection damaged mitochondrial morphology in coelomocytes and reduced mitochondrial membrane potential(ΔΨm)and mitophagosome *** colocalization of mitochondria and lysosomes further confirmed that lipopolysaccharide(LPS)treatment increased mitophagy *** explore the regulatory mechanism of mitophagy,we cloned Bcl2/adenovirus E1 B 19 kDa protein-interacting protein 3(BNIP3),a common mitophagy receptor,from sea cucumber Apostichopus japonicus(Aj BNIP3)and confirmed that Aj BNIP3 was significantly induced and accumulated in mitochondria after *** infection and LPS *** the mitochondrial membrane,Aj BNIP3 interacts with microtubule-associated protein 1 light chain 3(LC3)on phagophore membranes to mediate *** Aj BNIP3 interference,mitophagy flux decreased ***,Aj BNIP3-mediated mitophagy was activated by ROS following the addition of exogenous hydrogen peroxide(H2 O2),ROS scavengers,and ROS ***,inhibition of BNIP3-mediated mitophagy by Aj BNIP3 small interfering Rna(si Rna)or high concentrations of lactate increased apoptosis and decreased coelomocyte *** findings highlight the essential role of Aj BNIP3 in damaged mitochondrial degradation during *** mitophagy activity is required for coelomocyte survival in *** against *** infection.

关键词： Apostichopus japonicus Mitophagy Bcl2/adenovirus E1B 19 kDa protein-interacting protein 3 Reactive oxygen species Microtubule-associated protein 1 light chain 3

Mechanism of trifluoromethylation reactions with well-defined NHC copper trifluoromethyl complexes and iodobenzene:A computational exploration

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Chinese Chemical Letters 2015年第5期26卷 564-566页

作者： Dong-Hai Yu Jing-na shao Rong-Xing He Ming Li School of Chemistry and Chemical Engineering Southwest University

Computational calculation was performed to investigate the mechanism of trifluoromethylation reactions of iodobenzene with well-defined N-heterocyclic carbene （NHC）-supported Cu~ trifiuor- omethyl complexes. Four proposed reaction pathways, namely cr-bond metathesis （BM）, concerted oxidative addition-reductive elimination （OARE）, iodine atom transfer （IAT） and single-electron transfer （SET）, have been computed by density functional theory （DFT）. The result indicated that the concerted OARE mechanism is favored among the four reaction pathways, suggesting the trifluoromethylation may occur via concerted OARE mechanism involving Ar-X oxidative addition to the Cu（1） center as the rate determining step.

关键词： Trifluoromethylation Mechanism Density functional theory N-Heterocyclic carhene Trifluoromethyl copper complex

Cleaner production of ammonium paratungstate by membrane electrolysis-precipitation of sodium tungstate solution

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Tungsten 2023年第1期5卷 145-159页

作者： Afolabi Uthmon Olayiwola Hao Du shao-na Wang Biao Liu Ye-Qing Lv Bo Pan University of Chinese Academy of Sciences Beijing 100049China CAS Key Laboratory of Green Process and Engineering National Engineering Research Center of Green Recycling for Strategic Metal ResourcesInstitute of Process EngineeringChinese Academy of SciencesBeijing 100190China

The production of ammonium paratungstate(APT) is riddled with the generation of wastewater,which causes environmental *** solve the problem of wastewater generation at source,a membrane electrolysis-NH3·H2O precipitation method,which prevents wastewater generation and recycles the reagents used in the process,was proposed and investigated in this *** electrolysis process was investigated based on parameters such as initial cathodic and anodic naOH concentrations,and current *** results showed that an increase in current density and initial cathodic naOH concentration and a decrease in the initial anodic naOH concentration would enhance the separation of tungsten and *** optimum condition was found at a current density of 666 A·m^(-2),initial anodic and cathodic naOH concentrations of 69 g·L^(-1) and 40 g·L^(-1),with a current efficiency of 75.40%,and energy consumption for producing 1 ton of naOH was 2184 kW·*** precipitation process was investigated based on the acidic high W/na molar ratio solution obtained by the electrolysis process with NH3·H2O as the *** such as excessive coefficient,temperature,and W/na molar ratio were *** result showed that the variation of excessive coefficient and solution temperature had an opposite effect on the purity of the APT,while an increase in the W/na molar ratio would increase the product *** precipitation product obtained had a purity of 99.6% and was characterized using X-ray diffraction,inductively coupled plasma,and scanning electron *** methods proposed in this study could provide fundamental information for the design of a cleaner APT production process.

关键词： Membrane electrolysis Ammonium paratungstate Separation of sodium and tungsten ions Ion states of tungsten Cleaner production

Visualizing nitric oxide-dependent HIF-1 activity under hypoxia with a lipid droplet-targeting fluorescent probe

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Chinese Chemical Letters 2021年第12期32卷 3895-3898页

作者： Ying-Hao Pan Xiao-Xiao Chen Lei Dong na shao Li-Ya Niu Qing-Zheng Yang Key Laboratory of Radiopharmaceuticals Ministry of EducationCollege of ChemistryBeijing Normal UniversityBeijing 100875China

Evaluating the correlation between hypoxia inducible factor 1(HIF-1) and nitric oxide(NO) generated under hypoxia is of great significance. In this work, we developed a fluorescent probe for the monitor of HIF-1 activity influenced by NO under hypoxia in hepatoma cells with dual-targeting for hepatocyte and lipid droplet(LD). The probe shows excellent selectivity to NO and high sensitivity with 6000-fold fluorescence enhancement. Live cell imaging experiments revealed the probe’s capability of imaging exogenous and endogenous NO with specific in LDs of Hep G2 cells. For cells under hypoxia, HIF-1 induced LD level is observed to correlate with NO level. This work provides the in-situ visualization of NO-dependent HIF-1 upregulation through LD accumulation.

关键词： Bodipy Fluorescent probe HIF-1 Lipid droplet Nitric oxide

Integrated Development Path of Red Tourism and Rural Revitalization:A Case Study of Li Xiongcai's Hometown in Guangdong

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Asian Agricultural Research 2024年第12期16卷 1-7页

作者： na shao Sihua ZHANG School of Economics and Management Zhaoqing UniversityZhaoqing 526061China

With the increasing attention of the state to the protection and development and utilization of red tourism resources,a series of policy documents have been promulgated and implemented one after another,laying a solid policy and environmental foundation for the integrated development of red tourism and rural *** this context,this study takes"Li Xiongcai's hometown"in Kengwei Village,Baitu Town,Gaoyao District,Zhaoqing City,Guangdong Province,as a case study,adopts the questionnaire survey method to explore the current situation of the integration and development of red tourism and rural revitalization in five dimensions,namely,industry,culture,talent,ecology and organization,and summarizes the problems in the process of the integration and development of red tourism and rural revitalization and proposes a targeted optimization path to promote the development of red *** optimization paths are proposed to promote the high-quality development of red tourism in Li Xiongcai's hometown,to support the comprehensive revitalization of the countryside,and to provide model cases for other regions in the integration of red tourism and rural revitalization.

关键词： Red tourism Rural revitalization Integrated development Li Xiongcai's hometown

Research on Tourism Image Perception of Urban Ecological Scenic Areas Based on Analysis of Online Travel Notes: A case study of Baiyun Mountain Scenic Area in Guangzhou

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Journal of Landscape Research 2024年第2期16卷 81-86页

作者： shao na WU Zhenni WU Qing College of Economics&Management Zhaoqing UniversityZhaoqingGuangdong 526061China College of Tourism and Historical Culture Zhaoqing UniversityZhaoqingGuangdong 526061China

Taking Baiyun Mountain Scenic Area in Guangzhou as the research object,300 online travel notes were selected as research samples,and text analysis and ROST CM6 data analysis software were used to analyze tourists’image perception of Baiyun Mountain Scenic Area in Guangzhou from three aspects of cognitive image,emotional image and perceptual *** is found that:①in terms of cognitive image,tourists’perception of the tourist attractions is not balanced,and the service facilities such as catering,entertainment,shopping and accommodation are generally negative perceptions,while the perception of the tourism atmosphere tends to be positive.②In terms of emotional image,tourists have a positive attitude towards the overall evaluation of the scenic *** of these positive perceptions come from the natural landscapes and scenic spots of the scenic *** of the negative perception mainly comes from the lack of infrastructure,amusement facilities,service facilities or construction management,and the lag of maintenance management within the scenic area.③In terms of overall perception,the favorable rate accounts for 81%.The overall perception image of tourists is mainly concentrated in the three core vocabulary of“Baiyun Mountain Scenic Area”,“ticket”and“cable car”.At the same time,suggestions for improvement are proposed in four aspects,including integrating tourism resources in the scenic spot to improve the spatial balance of scenic spots in the scenic area,strengthening the maintenance of infrastructure construction and public environment to improve the service level of the scenic area,increasing the number of recreational tourism products and the entertainment experience of the scenic area,and deepening the reform of the ticket sales system to implement the combination of the set ticket system and the separate ticket system.

关键词： Perceptual image Text mining Online travel note Ecological tourism scenic area Baiyun Mountain Scenic Area

Syntheses, Structures and Photoluminescence of Novel Lanthanide-organic Frameworks Based on 1, 4-Bis(3,5-dicarboxylic pyrazol-1-ylmethyl) Benzene

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Chinese Journal of Structural Chemistry 2020年第5期39卷 960-966页

作者： LI Chuang HE Yong-Ke shao na XIONG Gang WU Shuang-Yan YOU Li-Xin SUN Ya-Guang The Key Laboratory of Inorganic Molecule-based Chemistry of Liaoning Province College of Applied ChemistryShenyang University of Chemical TechnologyShenyang 110142China

Two isomorphic 3D lanthanide metal-organic frameworks,{[Tb(L)0.5(OX)0.5(H2 O)2DMF]·DMF}n(1)and{[Dy(L)0.5(OX)0.5(H2 O)2 DMF]·DMF}n(2),have been prepared under solvothermal conditions based on l,4-bis(3,5-dicarboxylicpyrazol-1-ylmethyl)benzene(H4 L),oxalis acid(H2 OX)and lanthanide *** 1 crystallizes in monoclinic system,space group P21/c with a=9.418(15),b=15.23(2),c=14.47(2)?,β=100.07(3)°,V=2044(5)?3,Z=4,Mr=590.3 g/mol,Dc=1.919 g/cm^3,μ=3.524 mm-1,F(000)=1168,R=0.0893 and wR=0.1902 for 3486 observed reflections(I>2σ(I)).In compound 1,the central terbium(Ⅲ)ions are coordinated with eight atoms,and two neighboring terbium(Ⅲ)ions are linked together by one bridging oxalic acid group to give rise to a[Tb2(OX)(L)4]***[Tb2(OX)(L)4]unit connects to the surrounding six identical ones by the oxygen atoms bridging to lead to a 3D *** 1 exhibits strong luminescent emissions at the solid state owing to the antenna effect of the ligand.

关键词： lanthanide-organic frameworks luminescent emission antenna effect

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论技术与制度的互动关系

中州学刊 2017年第2期 7-12页

作者：邵娜南京大学政府管理学院扬州大学政府治理与公共政策研究中心

技术的二重性和结构的二重性共同决定了技术与制度之间的互动关系。技术在本质上具有客观属性和社会属性,这决定了技术既是社会行动的结果,也是社会行动的中介。结构的二重性则揭示了制度与行动之间的相互建构关系,表明结构是行动得以... 详细信息

技术的二重性和结构的二重性共同决定了技术与制度之间的互动关系。技术在本质上具有客观属性和社会属性,这决定了技术既是社会行动的结果,也是社会行动的中介。结构的二重性则揭示了制度与行动之间的相互建构关系,表明结构是行动得以展开的前提,行动是进行制度和结构再生产的活动。新技术的应用并不必然带来制度变革,当以内嵌于技术的新的规则、思维模式等因素为依托而产生新的行动模式时,制度变革的时机就到来了。组织领导者的思维模式应当从制度主义转向行动主义,以创造性的行动为中介,使技术应用成为一项实现制度变革的自觉行动,并为技术应用创造相应的制度空间。在高度复杂性、高度不确定性的条件下,网络技术的应用为官僚制组织向合作制组织转型提供技术支持。

关键词：结构化理论网络技术行动合作制组织网络结构