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Comparison of Viromes in Ticks from Different Domestic Animals in China

Virologica Sinica 2020年第4期35卷 398-406页

作者： Tingting Zhao Haiyan Gong Xiaojuan Shen Wen Zhang tongling shan Xiangqian Yu Seong Jin Wang Li Cui shanghai Key Laboratory of Veterinary Biotechnology School of Agriculture and BiologyShanghai Jiao Tong UniversityShanghai 200240China shanghai Veterinary Research Institute Chinese Academy of Agricultural SciencesShanghai 200241China shanghai Pudong New District Center for Animal Diseases Control and Prevention Shanghai 201299China School of Medicine Jiangsu UniversityZhenjiang 2012013China

Ticks are involved in the transmission of various arboviruses and some tick-borne viruses pose significant threats to the health of humans or *** study aimed to investigate the geographical distribution of tick species and tickassociated viruses in central and eastern *** 573 ticks from domestic animals including dogs,sheep and cattle were collected in *** genera of ticks were identified including Rhipicephalus and *** was performed on Miseq Illumina platform to characterize the tick viromes from the four different sampling *** trimming,13,640 reads were obtained and annotated to 19 virus *** these sequences,above 37.74%of the viral reads were related to the RNA *** comparison study revealed that the tick viral diversity was considerably different in the two identified tick *** viral diversity of *** was significantly different from that of other Rhipicephalus *** the other hand,substantial overlap in viral species was observed between the same *** addition,we found no evidence that the natural host played a major role in shaping virus diversity based on the comparison of their ***,the geographic location seems to significantly influence the viral *** study indicated that the novel negative-sense RNA viruses identified in this study was closely related to Bole tick virus 1 and 3 *** conclusion,the present study provides a baseline for comparing viruses detected in ticks,according to species,natural hosts,and geographic locations.

关键词： Ticks Viral metagenomics Virome

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Virome diversity of ticks feeding on domestic mammals in China

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Virologica Sinica 2023年第2期38卷 208-221页

作者： Zijun Yang Hao Wang Shixing Yang Xiaochun Wang Quan Shen Likai Ji Jian Zeng Wen Zhang Haiyan Gong tongling shan shanghai Veterinary Research Institute Chinese Academy of Agricultural SciencesShanghai200241China Department of Microbiology School of MedicineJiangsu UniversityZhenjiang212013China Department of Clinical Laboratory Huai'an HospitalXuzhou Medical UniversityHuai'an223002China Center of Clinical Laboratory The First Affiliated Hospital of Soochow UniversitySuzhou215006China

Ticks are considered the second most common pathogen vectors transmitting a broad range of vital human and veterinary *** 2017 to 2018,640 ticks were collected in eight different provinces in central and western *** species were detected,including ***,***,***,***,***,and ***-four viral metagenomic libraries were constructed on the MiSeq Illumina platform,resulting in 13.44 G(5.88×10^(7))of 250-bp-end reads,in which 2,437,941 are viral *** found 27 nearly complete genome sequences,including 16 genome sequences encoding entire protein-coding regions(lack of 30 or 50 end non-coding regions)and complete viral genomes,distributed in the arboviral family(Chuviridae,Rhabdoviridae,Nairoviridae,Phenuiviridae,Flaviviridae,Iflaviridae)as well as Parvoviridae and Polyomaviridae that cause disease in mammals and even *** addition,13 virus sequences found in Chuviridae,Nairoviridae,Flaviviridae,Iflaviridae,Hepeviridae,Parvoviridae,and Polyomaviridae were identified as belonging to a new virus species in the identified viral ***,an epidemiological survey shows a high prevalence(9.38%and 15.63%)of two viruses(Ovine Copiparvovirus and Bovine parvovirus 2)in the tick cohort.

关键词： Central and western China Viral metagenomes Tick-borne virus Phylogenetic analysis Domestic mammals Tick virome

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Host Interferon-Stimulated Gene 20 Inhibits Pseudorabies Virus Proliferation

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Virologica Sinica 2021年第5期36卷 1027-1035页

作者： Xiaoyong Chen Dage Sun Sujie Dong Huanjie Zhai Ning Kong Hao Zheng Wu Tong Guoxin Li tongling shan Guangzhi Tong Department of Swine Infectious Diseases Shanghai Veterinary Research InstituteChinese Academy of Agricultural SciencesShanghai 200241China Jiangsu Co-Innovation Center for the Prevention and Control of Important Animal Infectious Disease and Zoonose Yangzhou UniversityYangzhou 225009China

Host interferon-stimulated gene 20(ISG20)exerts antiviral effects on viruses by degrading viral RNA or by enhancing IFN ***,we examined the role of ISG20 during pseudorabies virus(PRV)*** found that ISG20 modulates PRV replication by enhancing IFN ***,ISG20 expression was upregulated following PRV infection and poly(I:C)*** expression of ISG20 inhibited PRV proliferation in PK15 cells,whereas knockdown of ISG20 promoted PRV *** addition,ISG20 expression upregulated IFN-βexpression and enhanced IFN downstream signaling during PRV ***,PRV UL24 suppressed the transcription of ISG20,thus antagonizing its antiviral *** domain mapping analysis showed that the N terminus(amino acids 1-90)of UL24 was responsible for the inhibition of ISG20 ***,these findings characterize the role of ISG20 in suppressing PRV replication and increase the understanding of host-PRV interplay.

关键词： Interferon-stimulated gene 20(ISG20) Interferon Pseudorabies virus(PRV) UL24

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Pangenome and multi-tissue gene atlas provide new insights into the domestication and highland adaptation of yaks

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Journal of Animal Science and Biotechnology 2024年第5期15卷 1832-1850页

作者： Daoliang Lan Wei Fu Wenhui Ji Tserang‑Donko Mipam Xianrong Xiong Shi Ying Yan Xiong Peng Sheng Jiangping Ni Lijun Bai tongling shan Xiangdong Kong Jian Li Ministry of Education of Key Laboratory of Qinghai‑Tibetan Plateau Animal Genetic Resource and Utilization Southwest Minzu UniversityChengduChina College of Animal&Veterinary Sciences Southwest Minzu UniversityChengduChina Institute of Qinghai‑Tibetan Plateau Southwest Minzu UniversityChengduChina Jiguang Gene Biotechnology Co. Ltd.NanjingChina Chengdu Genepre Technology Co. Ltd.ChengduChina shanghai Veterinary Research Institute Chinese Academy of Agricultural SciencesShanghaiChina

Background The genetic diversity of yak,a key domestic animal on the Qinghai-Tibetan Plateau(QTP),is a vital resource for domestication and breeding *** study presents the first yak pangenome obtained through the de novo assembly of 16 yak *** We discovered 290 Mb of nonreference sequences and 504 new *** pangenome-wide presence and absence variation(PAV)analysis revealed 5,120 PAV-related genes,highlighting a wide range of variety-specific genes and genes with varying frequencies across yak *** component analysis(PCA)based on binary gene PAV data classified yaks into three new groups:wild,domestic,and ***,we pro-posed a‘two-haplotype genomic hybridization model'for understanding the hybridization patterns among breeds by integrating gene frequency,heterozygosity,and gene PAV data.A gene PAV-GWAS identified a novel gene(Bos-Gru3G009179)that may be associated with the multirib trait in Jinchuan ***,an integrated transcrip-tome and pangenome analysis highlighted the significant differences in the expression of core genes and the muta-tional burden of differentially expressed genes between yaks from high and low *** analysis across multiple species revealed that yaks have the most unique differentially expressed m RNAs and lnc RNAs(between high-and low-altitude regions),especially in the heart and lungs,when comparing high-and low-altitude *** The yak pangenome offers a comprehensive resource and new insights for functional genomic studies,supporting future biological research and breeding strategies.

关键词： High-and low-altitude Novel genes Pangenome PAV-GWAS Yak

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Genetic characterization and pathogenicity of a reassortant Eurasian avian-like H1N1 swine influenza virus containing an internal gene cassette from 2009 pandemic H1N1 virus

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Virologica Sinica 2022年第4期37卷 627-630页

作者： Shuaiyong Wang Manzhu Wang Lingxue Yu Juan Wang Jiecong Yan Xinli Rong Yanjun Zhou tongling shan Wu Tong Guoxin Li Hao Zheng Guangzhi Tong Hai Yu shanghai Veterinary Research Institute Chinese Academy of Agricultural SciencesNo.518Ziyue RoadMinhang DistrictShanghai200241China Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses Yangzhou225009China

Dear editor,Swine influenza virus(SIV)is a member of the Orthomyxoviridae family,influenza A virus genus,which can cause the swine influenza—an acute and highly contagious respiratory disease in pigs(Brown,2000;Kothalawala et al.,2006).The SIV was first observed in 1918 in the United States and the progenitor of the SIV was the H1N1 influenza virus which caused the Spanish influenza pandemic of 1918(Shope,1931).

关键词： H1N1 swine Swine

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Suppression of Virulent Porcine Epidemic Diarrhea Virus proliferation by the PI3K/Akt/GSK-3/β pathway

Suppression of Virulent Porcine Epidemic Diarrhea Virus prol...

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中国畜牧兽医学会生物技术学分会暨中国免疫学会兽医免疫分会第十二次学术研讨会

作者： KONG Ning WU Yongguang MENG Qiong WANG Zhongze ZUO Yewen YANG Shen ZHOU En-min shan tongling TONG Guangzhi Department of Swine Infectious Disease Shanghai Veterinary Research InstituteChinese Academy of Agricultural Sciences Department of Preventive Veterinary Medicine College of Veterinary MedicineNorthwest A&F University

Porcine epidemic diarrhea(PED),caused by porcine epidemic diarrhea virus(PEDV),is an acute and highly contagious enteric disease of *** is characterized by watery diarrhea,vomiting and dehydration in sucking pigs,and the mortality rate as high as 100%for sucking pigs younger than 7 days of age after the virus *** 2010,various mu-

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Screening of Host Proteins Interacting with PorcineEpidemic Diarrhea Virus (PEDV) N Protein by YeastTwo-hybrid System

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Animal Husbandry and Feed Science 2018年第4期10卷 267-271页

作者： Wang Zhongze Qin Cuili Kong Ning Zuo Yewen Wang Meng Zheng Hao Tong Wu Li Liwei Yu Hai Li Zhili shan tongling Tong Guangzhi Li Xue Henan University of Science and Technology Luoyang 471023 China shanghai Institute of Veterinary Medicine Chinese Academy of Agricultural Sciences Shanghai 200241 China 不详

[Objective] The paper was to obtain host proteins interacting with porcine epidemic diarrhea virus （PEDV） N protein. [Method] The re-combinant vector pGBKT7-N of PEDV N gene was constructed and used as the bait plasmid to screen the proteins interacting with N protein ofPEDV from the cDNA library of porcine alveolar macrophage （PAM） by yeast two-hybrid method. [Result] There was no toxicity and self activationof bait protein in yeast hybridization system, and six proteins （FTH1, LGALS3, CORO1C, SNRPG, KRTAP5-3, ZNF598） interacting with N proteinwere indentified. It was confirmed that LGALS3 and SNRPG had specific interaction with N protein by return experiment and co-immunoprecipitation（CoIP） test. [Conclusion] The study lays a foundation for further studying the function of PEDV N protein and the pathogenic mechanism of PEDV.

关键词： Porcine epidemic diarrhea virus (PEDV) Yeast two-hybrid N protein Protein interaction

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PABPC4 Inhibits Coronavirus Proliferation by Degrading the Nucleocapsid

PABPC4 Inhibits Coronavirus Proliferation by Degrading the N...

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中国微生物学会兽医微生物学专业委员会暨中国畜牧兽医学会生物制品学分会2021年学术论坛

作者： Yajuan Jiao Ning Kong Hua Wang Dage Sun Sujie Dong Xiaoyong Chen Hao Zheng Wu Tong Hai Yu Yaowei Huang Huan Wang Baokun Sui Ling Zhao Ying Liao Wen Zhang Guangzhi Tong tongling shan

Emerging coronaviruses(CoVs) can cause severe diseases in humans and animals,but none of the currently available drugs or vaccines can effectively control these *** anti-viral proteinsplay an important role in inhibiting viral ***,we report that the host protein PABPC4 broadly inhibits the replication of CoVs belonging to four genera of the Coronaviridae family by targeting the nucleocapsid(N) protein to the autophagosomes for ***4 recruited the E3 ubiquitin ligase MARCH8 to the N protein for *** N protein was recognized by the cargo receptor NDP52,which delivered it to the autolysosomes for degradation,resulting in impaired viral *** data demonstrate that PABPC4 suppresses CoVs by degrading the N protein via the selective autophagy *** study provides useful information for the development of anti-coronaviral therapies.

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Virome comparisons in wild-diseased and healthy captive giant pandas

Virome comparisons in wild-diseased and healthy captive gian...

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第十三届全国野生动物生态与资源保护学术研讨会暨第六届中国西部动物学学术研讨会

作者： Wen Zhang Shixing Yang tongling shan Rong Hou Zhijian Liu Wang Li Lianghua Guo Yan Wang Peng Chen Xiaochun Wang Feifei Feng Hua Wang Chao Chen Quan Shen Chenglin Zhou Xiuguo Hua Li Cui Xutao Deng Zhihe Zhang Dunwu Qi Eric Delwart Department of Microbiology School of Medicine Jiangsu University Sichuan Key Laboratory of Conservation Biology for Endangered Wildlife Chengdu Research Base of Giant Panda Breeding Department of Swine Infectious Disease Shanghai Veterinary Research Institute Chinese Academy of Agricultural Sciences Department of Laboratory Medicine Jiangsu Taizhou People's Hospital School of Agriculture and Biology Shanghai Jiaotong University Blood Systems Research Institute Department of Laboratory Medicine University of California San Francisco

The giant panda(Ailuropoda melanoleuca)is a vulnerable mammal herbivore living wild in central *** infections have become a potential threat to the health of these endangered animals,but limited information related to these infections is ***:Using a viral metagenomic approach,we surveyed viruses in the feces,nasopharyngeal secretions,blood,and different tissues from a wild giant panda that died from an unknown

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A型塞内卡病毒3D蛋白原核表达及多克隆抗体制备

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中国动物传染病学报 2024年第5期32卷 191-195页

作者：程群孙大格翟雪滢杨心雨叶晨倩叶曼青孔宁单同领上海市闵行区动物疫病预防控制中心上海201109 中国农业科学院上海兽医研究所上海200241 不详

A型塞内卡病毒(SVA)是一种无囊膜的单股正链小RNA病毒,是塞内卡病毒属的唯一成员,其感染后可引起仔猪的急性死亡和母猪水泡样病变。SVA编码的非结构蛋白3D在病毒增殖及拮抗宿主天然免疫反应中发挥着重要的作用。本研究旨在原核表达SVA 3... 详细信息

A型塞内卡病毒(SVA)是一种无囊膜的单股正链小RNA病毒,是塞内卡病毒属的唯一成员,其感染后可引起仔猪的急性死亡和母猪水泡样病变。SVA编码的非结构蛋白3D在病毒增殖及拮抗宿主天然免疫反应中发挥着重要的作用。本研究旨在原核表达SVA 3D蛋白,免疫BALB/c小鼠制备多克隆抗体。以SVA RNA为模板,通过RT-PCR试验扩增3D基因,并将3D基因克隆至原核表达载体pCold-Ⅰ中,构建重组质粒3D-pCold-Ⅰ。将重组质粒转化至大肠杆菌BL21(DE3)感受态细胞,经IPTG诱导表达3D蛋白,纯化该重组蛋白后免疫BALB/c小鼠制备SVA 3D蛋白的多克隆抗体。结果显示,原核表达的SVA 3D蛋白是一种可溶性蛋白,其分子质量约为55 kDa。经Western blot和IFA试验鉴定,制备的SVA 3D多克隆抗体具有良好的反应性,本研究为SVA的基础研究和应用研究提供了工具。

关键词： A型塞内卡病毒 3D蛋白原核表达多克隆抗体

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