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Overexpression of proto-oncogene bcl-2.in rheumatoid synovium

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Chinese Science Bulletin 1997年第24期42卷 2087-2090页

作者： LIANG Haiying, wang yongchao, CHEN Zhiqiang, CHEN Huanmian and JIANG Ming Division of Rheumatology, Peking Union Medical College Hospital, {2. 100073, china 2. Key laboratory of cell Prolifer ation and Regulation biology, {2. normal university, {2. 100875, china Division of Rheumatology Peking Union Medical College Hospital Beijing China Key laboratory of cell Proliferation and Regulation {2. Beijing Normal University Beijing China

RHEUMATOID arthritis (RA) is an autoimmune disease which progressively and systematicallyinvades the joints of bones. A severe chronic situation may lead to the damage of cartilages,bones and tendons and eventually handicap the patients. One of the main histopathological fea-tures of rheumatoid synovium is a marked increase of the thickness and the number of cells inthe synovial lining layer. It is well known that the synovial lining cells secrete many cell fac-

关键词： rheumatoid synovium synovial lining cells bcI-2 apoptosis.

Primary evidence for involvement of IP3 in heat-shock signal transduction in Arabidopsis

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cell Research 2006年第4期16卷 394-400页

作者： Hong Tao Liu~(1,2.*) Fei Gao~(1,2.*) Shu Juan Cui~2.Jin Long Han~(1,2. Da ye Sun~2.Ren Gang Zhou~1 ~1 Institute of Genetics and Physiology,Hebei Academy of Agricultural Sciences,Shijiazhuang 050051,china ~2.nstitute of Molecular {2. biology,Hebei {2. university,Shijiazhuang 050016,china Institute of Genetics and Physiology Hebei Academy of Agricultural Sciences Shijiazhuang China Institute of Molecular cell biology Hebei Normal University Shijiazhuang China

The role of inositol 1,4,5-trisphosphate （IP3） in transducing heat-shock （HS） signals was examined in *** whole-plant IP3level increased within 1 min of HS at 37℃.After 3 min of HS,the IP3level reached a maximum2.5 fold *** the transgenic Arabidopsis plants that have AtHsp18.2.lvmoter-β-glucuronidase （GUS） fusiongene,it was found that the level of GUS activity was up-regulated by the addition of caged IP3at both non-HS and HStemperatures and was down-regulated by the phospholipase C （PLC） inhibitors {1-[6-((17β-3-Methoxyestra-1,3,5（10）-trien-17-yl)amino)hexyl]-2.5-pyrrolidinedione} （U-7312.）.The intracellular-free calcium ion concentration ([Ca2.]i) increased during HS at 37℃ in suspension-cultured Arabi-dopsis cells expressing *** with U-7312. prevented the increase of [Ca2.]ito some *** provided primary evidence for the possible involvement of IP3in HS signal transduction in higher plants.

关键词： heat shock signal transduction IP3 PLC [Ca2.]i Arabidopsis

Apoptosis of human leukemic HL-60 cells induced to differentiate by treatment with RA or DMSO

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cell Research 1995年第2期5卷 181-186页

作者： SUN HONG yongchao wang(Key Laborutory of {2. Proliferation and Regulation biology, {2. normal university, {2. 100875, china.) Key laboratory of cell Proliferation and Regulation {2. Beijing Normal University Beijing China

In present study we studied the effect of all-trans retinoic acid(ATRA) and dimethylsulfoxide (DMSO) on the induction of apoptosis in HL-60 cell line. Based on morphological changes by Hochest 33342.staining and identification of internucleosomal DNA cleavage by gel electrophoresis, we observed aberrant nuclear chromatin eondensation and ladder-like pattern of DNA degradation. Using Flow Cytometric method, we found sub-G1 peak in RA-treated HL-60 cells starting 5 to 6 d after the initiation of the treatment. However, such an obvious apoptotic peak was not identified in DMSO-differentiated cells. Combining the research accomplished before, our study approves further that apoptosis could be a common mode of death of terminally differentiated HL-60 cells.

关键词： Apoptosis HL-60 cell RA DMSO

A nonsynonymous SNP in human cytosolic sialidase in a small Asian population results in reduced enzyme activity: potential link with severe adverse reactions to oseltamivir

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cell Research 2007年第4期17卷 357-362页

作者： Chuan-Yun Li~(1,*) Quan Yu~(2.*) Zhi-Qiang ye~(1,*) Ying Sun~1 Quanyuan He~(1,3) Xiao-Mo Li~1 Wuxue Zhang~1 Jingchu Luo~1 Xiaocheng Gu~1 Xiaofeng Zheng~2.Liping Wei~1 ~1Center for Bioinformatics,~2.1} of Biochemistry and Molecular {2.,National {2. of Protein Engineering and Plant Genetic Engineering,College of Life Sciences,Peking university,beijing 100871,china ~3{2. of Biochemistry,College of Life Sciences,Hunan normal university,Changsha 410081,china Center for Bioinformatics National Laboratory of Protein Engineering and Plant Genetic Engineering College of Life Sciences Peking University Beijing China department of Biochemistry and Molecular biology National Laboratory of Protein Engineering and Plant Genetic Engineering College of Life Sciences Peking University Beijing China laboratory of Biochemistry College of Life Sciences Hunan Normal University Changsha China

The use of oseltamivir,widely stockpiled as one of the drugs for use in a possible avian influenza pandemic,has beenreported to be associated with neuropsychiatric disorders and severe skin reactions,primarily in *** we identi-fied a nonsynonymous SNP(single nucleotide polymorphism)in dbSNP database,R41 Q,near the enzymatic active siteof human cytosolic sialidase,a homologue of virus neuraminidase that is the target of *** SNP occurredin 9.2.% of Asian population and none of European and African American *** structural analyses and Kimeasurements using in vitro sialidase assays indicated that this SNP could increase the unintended binding affinity ofhuman sialidase to oseltamivir carboxylate,the active form of oseltamivir,thus reducing sialidase *** addition,this SNP itself results in an enzyme with an intrinsically lower sialidase activity,as shown by its increased Km anddecreased Vmax *** administration of oseltamivir to people with this SNP might further reduce theirsialidase *** note the similarity between the reported neuropsychiatric side effects of oseltamivir and the knownsymptoms of human sialidase-related *** propose that this Asian-enriched sialidase variation caused by theSNP,likely in homozygous form,may be associated with certain severe adverse reactions to oseltamivir.

关键词： Asia SNP neuraminidase inhibitor oseltamivir sialidase bioinformatics

Multi-endpoint assays reveal more severe toxicity induced by chloraminated effluent organic matter than chloraminated natural organic matter

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Journal of Environmental Sciences 2024年第1期135卷 310-317页

作者： Hai-Yan wang De-Xiu Wu ye Du Xiao-Tong Lv Qian-Yuan Wu State Key laboratory of Environmental Criteria and Risk Assessment Chinese Research Academy of Environmental SciencesBeijing 100012China Environmental Protection Key laboratory of Microorganism Application and Risk Control Guangdong Provincial Engineering Research Center for Urban Water Recycling and Environmental SafetyTsinghua Shenzhen International Graduate SchoolTsinghua UniversityShenzhen 518055China College of Architecture and Environment Sichuan UniversityChengdu 610000China

Disinfection by chloramination produces toxic byproducts and the difference in toxicity of reclaimed and drinking water treated by chloramination remains *** study investigated cytotoxic effects at the same concentrations of dissolved organic matter and showed that chloraminated effluent organic matter(EfOM)induced 1.7 times higher cytotoxicity than chloraminated natural organic matter(NOM)applied to simulate drinking *** EfOM induced more reactive nitrogen species than chloraminated NOM,and chloraminated EfOM and NOM induced similar and higher levels of reactive oxygen species than the negative control,***,intracellular macromolecule damage indicated by DNA/RNA damage marker 8–hydroxy-(deoxy)guanosine and the intracellular protein carbonyl concentration induced by chloraminated EfOM was higher and slightly more than that induced by chloraminated NOM,*** data were consistent with the effects on cell physiological *** cycle arrest mainly occurred in G2.phase by chloraminated EfOM and *** apoptotic cells,which could return to normal,increased upon exposure to high concentrations of chloraminated EfOM and ***,necrotic cells were significantly increased from 0.5%to 2.5%when the concentration increased from 2.-to 60-fold chloraminated EfOM,but were not obviously changed by chloraminated *** results indicated that the comprehensive intracellular changes induced by toxic substances in chloraminated EfOM were more irreversible and induced more cell death than chloraminated NOM.

关键词： Cytotoxicity Intracellular active nitrogen/oxygen Intracellular oxidative damage cell necrosis Reclaimed water

A novel aberrant splicing of G_(sa) transcript in human leukemia cell lines

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Chinese Science Bulletin 1999年第4期44卷 340-344页

作者： ye Qinong, YAO Xiao, wang Hengliang, ZHANG Shu, LIU Huaitian, SU Guofu, HUANG Cuifen and ZHOU Tingchong {2. Institute of Biotechnology, {2. 100071, china department of biology, Shaanxi normal College, Xi’an 710062. china {2. Medical university, School of Oncology, {2. 100036, china {2. Institute of Microbiology and Epidemiology, {2. 100071, china {2. Institute of Basic Medicine Sciences , {2. 100850, china beijing Institute of Biotechnology Beijing China department of biology Shaanxi Normal College Xi’an China School of Oncology Beijing Medical University Beijing China beijing Institute of Microbiology and Epidemiology Beijing China beijing Institute of Basic Medicine Sciences Beijing China

The α subunit of the stimulatory G protein, Gsα, is involved in the stimulation of adenylate cyclase pathway of signal transduction. The various Gsα mRNAs are generated either by alternative splicing or by using alternative promoter. A novel aberrant splicing of Gsα transcript in human leukemia cell lines is reported. The entire coding region of Gsα gene obtained, referred to as GsαLeu, was amplified by PCR with cDNA from Jurkat human leukemia cDNA library or the reverse-transcribed first strand cDNA from human leukemia cell line HL-60 as a template. The result of DNA sequencing indicated that,compared with originally reported Gsα, GsαLeu has two in-frame deletions, one in amino acid residues 2.—2.9 and the other in amino acid residues 2.4—2.0, suggesting that G（sαLeu） may play an important role in leukemia. The complete coding region of GsαLu was inserted downstream of the thrombin site of pGEX-2. fusion protein expression vector and expressed in the form of GST/G(

关键词： Gsa splicing leukemia cloning and expression.

Atomic Force Microscopy-based cell Nanostructure for Ligand-conjugated Quantum Dot Endocytosis

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Acta Biochimica et Biophysica Sinica 2006年第9期38卷 646-652页

作者： Yun-Long PAN~1* Ji-ye CAI~2.Li QIN~3 Hao wang~4 1 The First Affiliated Hospital of Jinan {2.,Guangzhou 510632.china 2.College of Life Science and Technology,Jinan {2.,Guangzhou 510632.china 3 department of Histology and Embryology,Medical College of Jinan {2.,Guangzhou 510632.china 4 Information and Optoelectronic Science and Engineering.South china normal {2.,Guangzhou 510631,china The First Affiliated Hospital of Jinan universityGuangzhou 510632 China College of Life Science and Technology Jinan UniversityGuangzhou 510632 China department of Histology and Embryology Medical College of Jinan UniversityGuangzhou 510632 China School for Information and Optoelectronic Science and Engineering South China Normal UniversityGuangzhou 510631 China

While it has been well demonstrated that quantum dots (QDs) play an important role inbiological labeling both in vitro and in vivo,there is no report describing the cellular nanostructure basis ofreceptor-mediated ***,nanostructure evolution responses to the endocytosis of transferrin(Tf)-conjugated QDs were characterized by atomic force microscopy (AFM).AFM-based nanostructureanalysis demonstrated that the Tf-conjugated QDs were specifically and tightly bound to the cell receptorsand the nanostructure evolution is highly correlated with the cell membrane receptor-mediated ***,confocal microscopic and flow cytometry results have demonstrated the specificity anddynamic property of Tf-QD binding and *** found that the internalization of Tf-QD is linearlyrelated to ***,while the nanoparticles on the cell membrane increased,the endocytosis was stillvery active,suggesting that QD nanoparticles did not interfere sterically with the binding and function ***,ligand-conjugated QDs are potentially useful in biological labeling of cells at a nanometerscale.

关键词： quantum dot endocytosis nanostructure atomic force microscopy

Self-shrinking supramolecular nanoparticles syndicate energy suppression and NIR-II mild photothermal amplification of mitochondrial oxidative stress for breast cancer therapy

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Nano Research 2024年第5期17卷 4314-4328页

作者： Hongmei Liu Jiming Xu Mengjie ye Hengbo Zhang Linlin Han Jingting wang Peng Xue Yuejun Kang Zhigang Xu Key laboratory of Luminescence Analysis and Molecular Sensing Ministry of EducationSchool of Materials and Energy&Chongqing Engineering Research Center for Micro-Nano Biomedical Materials and DevicesSouthwest UniversityChongqing 400715China Key laboratory of Laser Technology and Optoelectronic Functional Materials of Hainan Province College of Chemistry and Chemical EngineeringHainan Normal UniversityHaikou 571158China Yibin Academy of Southwest university Yibin 644000China

Photothermal therapy(PTT)may lead to healthy tissue damage,tumor metastasis,and recurrence,which makes mild photothermal therapy(mild PTT)stand ***,overcoming heat resistance,insufficient therapeutic effect,and poor photothermal conversion efficiency has become new ***,we report a dynamic supramolecular nanocarrier formed from amide-sericin and aldehyde-polyhydroxy glucan(denoted as SDA),the loose cavity of which can be filled by using the pharmaceutical combination of lonidamine(LND)and NIR-II photothermal agent of IR-1061,producing SDLI with a tighter inner hole,smaller and uniform particle size and excellent stability due to multiple pulling ***,the intricate internal network structure prevents the hydrophobic IR-1061 from forming aggregates in the small cavity,and the photothermal conversion efficiency(PCE)can reach 48.9%.At the acidic tumor microenvironment of pH 6.5,the controlled release of LND can solve the problem of heat resistance of NIR-II mild PTT and significantly improve the therapeutic effect of NIR-II mild ***,SDLI also shows a reasonable tumor inhibition rate,so the synergistic strategy of inhibiting tumor energy metabolism and NIR-II mild PTT to magnify mitochondrial oxidative stress,continuous cell stress state-induced immunogenic cell death to promote the induction of tumor apoptosis is proposed to achieve more effective cancer treatment.

关键词： self-shrinkage dynamic supramolecular nanoparticles tumor energy metabolism NIR-II mild photothermal therapy mitochondrial oxidative stress

Pan-cancer analysis of RNA 5-methylcytosine reader (ALYREF)

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Oncology Research 2024年第3期32卷 503-515页

作者： XING ye ZHOUTING TUO KAI CHEN RUICHENG WU JIE wang QINGXIN YU LUXIA ye AKIRA MIYAMOTO KOO HAN YOO CHI ZHANG WURAN WEI DENGXIONG LI DECHAO FENG Samuel Oschin Comprehensive Cancer Institute Department of MedicineDepartment of Biomedical SciencesCedars-Sinai Medical CenterLos AngelesCA90048USA department of Urology The Second Afiliated Hospital of Anhui Medical UniversityHefei230601China department of Urology Institute of UrologyWest China HospitalSichuan UniversityChengdu610041China department of Pathology Ningbo Diagnostic Pathology Center Ningbo315021China department of Public Research Platfom Taizhou Hospital of Zhejiang Province Afliated to Wenzhou Medical UniversityLinhai317000China department of Rehabilitation West Kyushu UniversityKanzaki shi842-8585Japan department of Urology Kyung Hee UniversitySeoul446701South Korea department of Rehabilitation The Afliated Hospital of Southwest Medical UniversityLuwzhou646000China

The incre asing interest in RNA modifications has signifcantly advanced epigenomic and epitranscriptomic *** study focuses on the immuno oncological impact of ALYREF in human cancer through a pan-cancer analysis,enhancing understanding of this gene's role in *** observed differential ALYREF expression between tumor and normal samples,correl ating strongly with prognosis in various cancers,particularly kidney renal papillary cell carcinoma(KIRP)and liver hepatocellular carcinoma(LIHC).ALYREF showed a negative correlation with most tumor-infitrating cells in lung squamous cell carcinoma(LUSC)and lymphoid neoplasm difuse large B-cell lymphoma(DLBC),while positive correlations were noted in IIHC,kidney chromophobe(KICH),mesothelioma(MESO),KIRP,pheochromocytoma and paraganglioma(PARD),and glioma(GBMLGG).Aditionally,ALYREF expression was closely associated with tumor heterogeneity,stemness indices,and a high mutation rate in TP53 across these *** conclusion,ALYREF may serve as an oncogenic biomarker in numerous cancers,meriting further research attention.

关键词： Pan-cancer RNA 5-methylcytosine ALYREF Immwno-oncological efects