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Immobilization of Lysozyme on Biomass Charcoal Powder Derived from Plant Biomass Wastes

Journal of Biomaterials and Nanobiotechnology 2012年第4期3卷 446-451页

作者： Hidetaka Noritomi Reona Ishiyama ryotaro kai Daiki Iwai Masahiko Tanaka Satoru Kato Department of Applied Chemistry Tokyo Metropolitan University Tokyo Japan EEN Co. Ltd. Tokyo Japan

Biomass charcoal powder (BCP) was used as a carrier matrix for immobilization of chicken egg white lysozyme. BCP was derived from plant biomass wastes such as dumped adzuki beans by pyrolysis without combustion under nitrogen atmosphere and grinding with a jet mill. The amount of lysozyme immobilized on BCP of adzuki beans by adsorption was 11 μmol/g (0.16 g/g) at pH 7.0. The optimum pH values for free and immobilized lysozyme activities were 6.8 and 7.2, respectively. The optimum temperature for both free and immobilized lysozyme activities was 25℃. The half-life of immobilized lysozyme exhibited 1.8-fold compared to that of free lysozyme at 5℃. Moreover, the half life of immobilized lysozyme was 7 times greater than that of lysozyme at 90℃.

关键词： Immobilization Biomass Charcoal Powder Lysozyme Activity Stability

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Thermostability of lysozyme immobilized on biomass charcoal powder

Thermostability of lysozyme immobilized on biomass charcoal ...

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The 12th Japan-China-Korea Joint Symposium on Enzyme Engineering

作者： Hidetaka Noritomi ryotaro kai Masahiko Tanaka Satoru Kato Department of Applied Chemistry Tokyo Metropolitan University

＜正＞Introduction Proteins are biomolecules of great importance in the medical,pharmaceutical,and food fields,since they exhibit their outstanding biological activities under mild ***,most of proteins dissolved in an aqueous solution are immediately denatured and inactivated at high *** particular,an aggregation of denatured proteins becomes the major problem because of the fast irreversible

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Increase in thermal stability of proteins adsorbed on biomass charcoal powder prepared from plant biomass wastes

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Journal of Biomedical Science and Engineering 2011年第11期4卷 692-698页

作者： Hidetaka Noritomi ryotaro kai Daiki Iwai Hirotaka Tanaka Reo Kamiya Masahiko Tanaka Kohichiroh Muneki Satoru Kato 不详

Thermal stability of lysozyme adsorbed on biomass charcoal powder (BCP), which was prepared from plant biomass wastes such as dumped adzuki bean, bamboo, and wood by pyrolysis without combustion under nitrogen atmosphere and comminution with a jet mill, was examined. Adsorbing lysozyme on BCP could sufficiently prevent proteins from denaturing and aggregating in an aqueous solution at high temperatures, and enhanced the refolding of thermally denatured proteins by cooling treatment. The remaining activities of lysozyme adsorbed on BCP of adzuki bean exhibited 51% by cooling treatment after the heat treatment at 90?C for 30 min, although that of native lysozyme was almost lost under the same experimental conditions. The thermostabilization effect of BCP on the remaining activity of adsorbed lysozyme was markedly dependent upon the kind of plant biomass wastes.

关键词： Adsorption Biomass Charcoal Powder Lysozyme Refolding Remaining Activity Thermal Stability

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