Increase in thermal stability of proteins adsorbed on biomass charcoal powder prepared from plant biomass wastes
Increase in thermal stability of proteins adsorbed on biomass charcoal powder prepared from plant biomass wastes作者机构：不详
出 版 物：《Journal of Biomedical Science and Engineering》 (生物医学工程（英文）)
年 卷 期：2011年第4卷第11期
页 面：692-698页
学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学]
主 题：Adsorption Biomass Charcoal Powder Lysozyme Refolding Remaining Activity Thermal Stability
摘 要：Thermal stability of lysozyme adsorbed on biomass charcoal powder (BCP), which was prepared from plant biomass wastes such as dumped adzuki bean, bamboo, and wood by pyrolysis without combustion under nitrogen atmosphere and comminution with a jet mill, was examined. Adsorbing lysozyme on BCP could sufficiently prevent proteins from denaturing and aggregating in an aqueous solution at high temperatures, and enhanced the refolding of thermally denatured proteins by cooling treatment. The remaining activities of lysozyme adsorbed on BCP of adzuki bean exhibited 51% by cooling treatment after the heat treatment at 90?C for 30 min, although that of native lysozyme was almost lost under the same experimental conditions. The thermostabilization effect of BCP on the remaining activity of adsorbed lysozyme was markedly dependent upon the kind of plant biomass wastes.
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