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First report of polymicrobial necrotizing fasciitis caused by Eggerthia catenaformis and Finegoldia magna

World Journal of Emergency Medicine 2022年第4期13卷 326-329页

作者： Claudius Illg Jonas Kolbenschlag ruth christine schäfer Adrien Daigeler Sabrina Krauss Department of Hand Plastic and Reconstructive SurgeryBG Unfallklinik TuebingenEberhard Karls UniversityTuebingen 72076Germany

Dear editor,Necrotizing fasciitis is a relatively rare,rapidly advancing,infectious disease with high lethality.[1]It is characterized by subcutaneous and fascial tissue necrosis and can even extend to involve skin and muscle.[2]The initial spread along fascial planes with little visual evidence impedes the crucial early *** often report minor or major trauma preceding the infection,yet in some cases,the disease develops idiopathically.[3]Risk factors,including older age,diabetes,chronic renal failure,and obesity,predispose to impaired immunity and loss of physiological barriers.[2]

关键词： impaired immunity fas

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Characterization of the osteogenic potential of mesenchymal stem cells from human periodontal ligament based on cell surface markers

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International Journal of Oral Science 2015年第4期7卷 213-219页

作者： ruth Alvarez Hye-Lim Lee Cun-Yu Wang christine Hong Division of Oral Biology and Medicine School of DentistryUniversity of California at Los Angeles Section of Orthodontics School of DentistryUniversity of California at Los Angeles

Mesenchymal stem cell （MSC）-mediated therapy has been shown to be clinically effective in regenerating tissue defects. For improved regenerative therapy, it is critical to isolate homogenous populations of MSCs with high capacity to differentiate into appropriate tissues. The utilization of stem cell surface antigens provides a means to identify MSCs from various tissues. However, few surface markers that consistently isolate highly regenerative MSCs have been validated, making it challenging for routine clinical applications and making it all the more imperative to identify reliable surface markers. In this study, we used three surface marker combinations： CD51/CD140a, CD271, and STRO-1/CD146 for the isolation of homogenous populations of dental mesenchymal stem cells （DMSCs） from heterogeneous periodontal ligament cells （PDLCs）. Fluorescence-activated cell sorting analysis revealed that 24% of PDLCs were CD51＋/CD140a＋, 0.8% were CD271＋, and 2.4% were STRO-1＋/CD146＋. Sorted cell populations were further assessed for their multipotent properties by inducing osteogenic and chondrogenic differentiation. All three subsets of isolated DMSCs exhibited differentiation capacity into osteogenic and chondrogenic lineages but with varying degrees. CD271＋ DMSCs demonstrated the greatest osteogenic potential with strong induction of osteogenic markers such as DLX5, RUNX2, and BGLAP. Our study provides evidence that surface marker combinations used in this study are sufficient markers for the isolation of DMSCs from PDLCs. These results provide important insight into using specific surface markers for identifying homogenous populations of DMSCs for their improved utilization in regenerative medicine.

关键词： cell surface markers dental mesenchymal stem cells periodontal ligament

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维普期刊数据库

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Single CD271 marker isolates mesenchymal stem cells from human dental pulp

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International Journal of Oral Science 2015年第4期7卷 205-212页

作者： ruth Alvarez Hye-Lim Lee christine Hong Cun-Yu Wang Division of Oral Biology and Medicine School of DentistryUniversity of California at Los Angeles Section of Orthodontics School of DentistryUniversity of California at Los Angeles Department of Bioengineering Henry Samueli School of Engineering and Applied ScienceUniversity of California at Los Angeles

Mesenchymal stem cells （MSCs） are a promising tool in regenerative medicine due to their capacity to differentiate into multiple lineages. In addition to MSCs isolated from bone marrow （BMSCs）, adult MSCs are isolated from craniofacial tissues including dental pulp tissues （DPs） using various stem cell surface markers. However, there has been a lack of consensus on a set of surface makers that are reproducibly effective at isolating putative multipotent dental mesenchymal stem cel^s （~M^Cs）. II1 ~his stucly, we used clif^et（~nt combinations of surface markers （CD51/CD140a, CD271, and STRO-1/CD146） to isolate homogeneous populations of DMSCs from heterogeneous dental pulp cells （DPCs） obtained from DP and compared their capacity to undergo multilineage differentiation. Fluorescence-activated cell sorting revealed that 27.3% of DPCs were CD51＋/CD140a＋, 10.6% were CD271＋, and 0.3% were STRO-1＋/CD146＋. Under odontogenic conditions, all three subsets of isolated DMSCs exhibited differentiation capacity into odontogenic lineages. Among these isolated subsets of DMSCs, CD271＋ DMSCs demonstrated the greatest odontogenic potential. While all three combinations of surface markers in this study successfully isolated DMSCs from DPCs, the single CD271 marker presents the most effective stem cell surface marker for identification of DMSCs with high odontogenic potential. Isolated CD271＋ DMSCs could potentially be utilized for future clinical applications in dentistry and regenerative medicine.

关键词： dental mesenchymal stem cells odontogenic differentiation cell surface markers dental pulp fluorescence-activated cellsorting

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