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RNA interference in insects:the link between antiviral defense and pest control

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Insect Science 2024年第1期31卷 2-12页

作者： jinzhi Niu ruoyu Chen jin-Jun Wang Key Laboratory of Entomology and Pest Control Engineering College of Plant ProtectionSouthwest UniversityChongqingChina Key Laboratory of Agricultural Biosafety and Green Production of Upper Yangtze River(Ministry of Education) Southwest UniversityChongqingChina

RNA interference(RNAi)is a form of gene silencing triggered by double-stranded RNA(dsRNA)that operates in all eukaryotic *** has been widely investigated in insects to determine the underlying molecular mechanism,to investigate its role in systemic antiviral defense,and to develop strategies for pest *** insect cells are infected by viruses,viral dsRNA signatures trigger a local RNAi response to block viral replication and generate virus-derived DNA that confers systemic ***-based insect pest control involves the application of exogenous dsRNA targeting genes essential for insect development or survival,but the efficacy of this approach has limited potency in many pests through a combination of rapid dsRNA degradation,inefficient dsRNA uptake/processing,and ineffective RNAi *** could be addressed by dsRNA screening and evaluation,focusing on dsRNA design and off-target management,as well as dsRNA production and *** review summarizes recent progress to determine the role of RNAi in antiviral defense and as a pest control strategy in insects,addressing gaps between our fundamental understanding of the RNAi mechanism and the exploitation of RNAi-based pest control strategies.

关键词： RNAi response systemic RNAi insecticidal dsRNA biological control off-targets

High-yield and rapid isolation of extracellular vesicles by flocculation via orbital acoustic trapping:FLOAT

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Microsystems & Nanoengineering 2024年第1期10卷 313-323页

作者： Joseph Rufo Peiran Zhang Zeyu Wang Yuyang Gu Kaichun Yang Joseph Rich Chuyi Chen ruoyu Zhong Ke jin Ye He Jianping Xia Ke Li Jiarong Wu Yingshi Ouyang Yoel Sadovsky Luke P.Lee Tony Jun Huang Department of Mechanical Engineering and Materials Science Duke UniversityDurhamNCUSA Department of Biomedical Engineering Duke UniversityDurhamNCUSA Department of Obstetrics Gynecologyand Reproductive SciencesMagee-Womens Research InstituteUniversity of PittsburghPittsburghPAUSA Department of Microbiology and Molecular Genetics School of MedicineUniversity of PittsburghPittsburghPAUSA Renal Division and Division of Engineering in Medicine Department of MedicineBrigham and Women’s HospitalHarvard Medical SchoolBostonMAUSA Department of Bioengineering Department of Electrical Engineering and Computer ScienceUniversity of CaliforniaBerkeleyBerkeleyCAUSA Department of Biophysics Institute of Quantum BiophysicsSungkyunkwan UniversitySuwonKorea

Extracellular vesicles(EVs)have been identified as promising biomarkers for the noninvasive diagnosis of various ***,challenges in separating EVs from soluble proteins have resulted in variable EV recovery rates and low ***,we report a high-yield(>90%)and rapid(<10 min)EV isolation method called FLocculation via Orbital Acoustic Trapping(FLOAT).The FLOAT approach utilizes an acoustofluidic droplet centrifuge to rotate and controllably heat liquid *** adding a thermoresponsive polymer flocculant,nanoparticles as small as 20 nm can be rapidly and selectively concentrated at the center of the *** demonstrate the ability of FLOAT to separate urinary EVs from the highly abundant Tamm-Horsfall protein,addressing a significant obstacle in the development of EV-based liquid *** to its high-yield nature,FLOAT reduces biofluid starting volume requirements by a factor of 100(from 20 mL to 200µL),demonstrating its promising potential in point-of-care diagnostics.

关键词： trapping utilize separating

SiOx/C-Ag nanosheets derived from Zintl phase CaSi_(2) via a facile redox reaction for high performance lithium storage

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Nano Research 2022年第1期15卷 395-400页

作者： Jie Xie Lin Sun Yanxiu Liu Xinguo Xi ruoyu Chen Zhong jin Jiangsu Province Cultivation Base for State Key Laboratory of Photovoltaic Science and Technology School of Petrochemical EngineeringChangzhou UniversityChangzhou 213164China School of Chemistry and Chemical Engineering Yancheng Institute of TechnologyYancheng 224051China Key Laboratory of Mesoscopic Chmistry of Ministry of Education(MOE) School of Chemistry and Chemical EngineeringNanjing UniversityNanjing 210023China

As one of the high-capacity anodes in lithium-ion batteries(LIBs),silicon oxide(SiOx)has attracted wide attention due to its high theoretical capacity,low cost,and proper working ***,the huge volume change and the intrinsic poor conductivity of SiOx still hinder the practical *** to address the issues is the focus of current *** this work,firstly,hydrogen passivated Si nanosheets(Si6H6)were prepared from Zintl phase CaSi2,then,two-dimensional Ag nanoparticle modified SiOVC nanocomposite was prepared via a facile complex redox reaction between SieH6 and AgN03-aniline complexing *** this design,aniline was served as carbon sources,and Si6H6could be transformed to SiOx by AgN03 in mild solution *** obtained Ag modified SiOVC(SiOx/C-Ag)electrode exhibited high specific capacity(550 mAh·g^(-1)at 0.6 A·g^(-1)),superior rate,and cycling performance when served as anode for LIBs.

关键词： SiOx anode LIBs redox reaction two-dimensional structure solution synthesis

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基于知识蒸馏的步态识别方法

云南师范大学学报（自然科学版） 2024年第2期44卷 49-53页

作者：李若愚云利军金雪松杨彦辰程飞燕云南师范大学信息学院云南昆明650500 云南省教育厅计算机视觉与智能控制技术工程研究中心云南昆明650500 玉溪市第二人民医院信息网络中心云南玉溪653100

针对步态识别中网络模型复杂度高、参数量大、训练测试速度慢等问题,提出一种基于知识蒸馏的步态识别方法.通过知识蒸馏方法对ConvNext-KD模型进行训练,在不增加新训练数据集、模型复杂度和模型参数量的前提下,提高ConvNext-KD模型的识... 详细信息

针对步态识别中网络模型复杂度高、参数量大、训练测试速度慢等问题,提出一种基于知识蒸馏的步态识别方法.通过知识蒸馏方法对ConvNext-KD模型进行训练,在不增加新训练数据集、模型复杂度和模型参数量的前提下,提高ConvNext-KD模型的识别准确率.该方法在中科院CASIA-B和CASIA-C数据库中进行多次仿真实验.结果表明,ConvNext-KD模型在保持较小参数量和较低复杂度的同时,可以显著缩短训练测试的时长并取得较高识别准确率.

关键词：步态识别知识蒸馏 ConvNext

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BIM技术应用的法律问题:国外研究综述

土木建筑工程信息技术 2019年第6期11卷 36-41页

作者：刘枬张媛杰邓禾金若愚重庆交通大学经济与管理学院重庆400074 西南政法大学经济法学院重庆401120 英国布莱顿大学环境与工程学院布莱顿英国BN24GJ

随着信息化的演进,BIM技术正以其独特优势在工程领域迅速普及和推广。然而,作为一项发展中的新技术,BIM在实际应用中存在多方面的障碍,其中,法律问题引起了研究者和从业人员的重视,但目前在国内却没有相应的研究成果。因此,本文采用文... 详细信息

随着信息化的演进,BIM技术正以其独特优势在工程领域迅速普及和推广。然而,作为一项发展中的新技术,BIM在实际应用中存在多方面的障碍,其中,法律问题引起了研究者和从业人员的重视,但目前在国内却没有相应的研究成果。因此,本文采用文献综述的研究方法,从权属关系、责任和合同三个方面,梳理了国外研究BIM应用中出现的法律问题所取得的成果,为今后在中国语境下开展这一领域的研究和司法实践打下基础。

关键词：建筑信息化 BIM 法律问题

基于荆楚文化IP的文创产品设计应用研究——以“楚式鬲”角色及其衍生产品为例

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宝石和宝石学杂志（中英文） 2023年第1期25卷 57-65页

作者：金若雨张荣红武汉设计工程学院时尚设计学院湖北武汉430205 中国地质大学(武汉)珠宝学院湖北武汉430074

有着近3000年历史的荆楚文化,为湖北省文创产品开发提供了丰厚的物质财富和精神资源。现有荆楚文化文创产品市场存在经营模式较为单一保守、产品种类相对同质化、缺乏经典IP打造等问题,亟待设计师整合文物资源,深入发掘荆楚优秀文化资源... 详细信息

有着近3000年历史的荆楚文化,为湖北省文创产品开发提供了丰厚的物质财富和精神资源。现有荆楚文化文创产品市场存在经营模式较为单一保守、产品种类相对同质化、缺乏经典IP打造等问题,亟待设计师整合文物资源,深入发掘荆楚优秀文化资源,积极开展活化利用。IP作为具有强变现穿透能力、长变现周期的文化符号,对文创产品开发具有激发价值认同、精准定位用户群体、多维融合促使传播变现等作用。通过分析“楚式鬲”作为原型构建IP的可行性,从视觉符号提取和人格化设定等方面入手,确定“鬲鬲(Lili)”角色IP的视觉形象和背景故事,并从多维度开展线上数字衍生品和线下实体衍生品设计,探讨IP属性文创产品的设计应用方法,从而为充分发掘荆楚文化与产品设计的契合点提供新的思路,找到以荆楚文化特色作为创新源头进行设计的新的方法,借助IP这一载体激发消费者的文化认同感,实现文脉传承与弘扬的目标。

关键词：荆楚文化角色IP 楚式鬲文创产品设计

Research on the Response Characteristics of Solenoid Valve of the Air-jet Loom by Simulation

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Journal of Thermal Science 2013年第6期22卷 606-612页

作者： jin Yuzhen DENG ruoyu jin Yingzi HU Xudong Faculty of Mechanical Engineering & Automation Zhejiang Sci-Tech University

Solenoid valve is one of the executive parts of weft insertion control system. According to the response characteristics of the solenoid valve, an improved design becomes a necessity. Firstly, the numerical model was established after analyzing the solenoid valve during its start-up and shut-down. Comparing the simulation data with the practical data, it is verified that the numerical simulation model has a high feasibility. Secondly, excitation voltage and spring pre-compression were adjusted respectively, and the response rules after adjusting were investigated. The research of the study shows: the response time tends to be inverse proportional to the excitation voltage during start-up, and it becomes a constant value with the increase of the excitation voltage; the response time is proportional to the spring pre-compression when the solenoid valve starts up, it is inverse proportional to spring pre-compression when the solenoid valve shuts down. And the total response time is a constant value with the increase of the spring pre-compression. Therefore, the value of the excitation voltage and the spring pre-compression should be selected when the curve is becoming flatten. The results of the research can provide the reference to the further development of the solenoid valve.

关键词：数值模拟模型电磁阀响应特性喷气织机激励电压地球化学特征反应时间预压缩

人龋病牙髓中神经肽与抗原呈递细胞的动态研究

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天津医药 2004年第3期32卷 132-135页

作者：郝玉梅郝占国杨若愚靳立巾武警医学院附属医院五官科 300162 武警医学院训练部天津医科大学口腔医院

目的 :研究人龋病牙髓中降钙素基因相关肽 (Calicitoningene -relatedpeptide,CGRP)和树突状细胞(Dendriticcells,DC)、P物质 (SubstanceP,SP)和血管内皮细胞(Endothelialcells,EC)之间的空间变化特点 ,以探明牙髓病理状态下神经免疫间的相互作用。方法:采用免疫组化法观察龋病不同阶段牙髓中上述物质的变化 ,并对前、后两种物质分别进行双标免疫组化染色。结果:(1)4个指标的数量在不同组间比较差别有统计学意义 (P<0.05)。 (2)双标染色显示 :龋病牙髓中神经肽与抗原呈递细胞共聚集在龋坏相应冠髓的造牙本质细胞层下 ,CGRP接触或包绕树突状细胞 ;而SP随龋坏深入逐渐接近血管并与管壁接触。结论:龋源性刺激引起牙髓中CGRP、SP、DC。

关键词：牙髓神经肽动态研究龋齿免疫组织化学降钙素基因相关肽 P物质树突细胞

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四川省常见树种防火期凋落物燃烧性分析

防护林科技 2024年第3期 50-55页

作者：钟金朱宇颐覃方兴何晓成阮若玉贾娟崔兴雷四川省林业和草原调查规划院四川成都610081 龙门山生物多样性保护省级长期科研基地四川成都610081 四川省森林和草原防火监测中心四川成都610081 四川农业大学林学院国家林业草原西南森林与草原生态防火工程技术研究中心四川成都625014

为防火树种的选择提供理论依据,搜集四川省59种常见树种防火期的凋落物,对凋落物的载量、含水率、粗脂肪含量、燃点、热值、灰分进行测定,使用主成分分析评价不同树种凋落物的燃烧性。结果表明:不同树种凋落物载量和含水率差异显著。凋... 详细信息

为防火树种的选择提供理论依据,搜集四川省59种常见树种防火期的凋落物,对凋落物的载量、含水率、粗脂肪含量、燃点、热值、灰分进行测定,使用主成分分析评价不同树种凋落物的燃烧性。结果表明:不同树种凋落物载量和含水率差异显著。凋落物燃烧性较高的树种有高山松、柏木等;燃烧性较低的有黄葛树、杧果等。凋落物燃烧性低的树种可选为潜在防火树种。

关键词：四川燃烧性凋落物主成分分析防火树种

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