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Signal Transduction and Targeted Therapy 2021年第9期6卷 2870-2882页

作者： ruoxi xie Zijun Wu Fanxin Zeng Huawei Cai Dan Wang Lei Gu Hongyan Zhu Su Lui Gang Guo Bin Song Jinxing Li Min Wu Qiyong Gong Huaxi MR Research Center(HMRRC) Department of RadiologyFunctional and Molecular Imaging Key Laboratory of Sichuan ProvinceWest China HospitalSichuan UniversityChengduChina Research Unit of Psychoradiology Chinese Academy of Medical SciencesChengduChina Department of Clinic Medical Center Dazhou Central HospitalDazhouChina Laboratory of Clinical Nuclear Medicine Department of Nuclear MedicineWest China HospitalSichuan UniversityChengduChina State Key Laboratory of Biotherapy and Cancer Center West China HospitalSichuan Universityand Collaborative Innovation Center for BiotherapyChengduChina Department of Chemical Engineering Stanford UniversityStanfordCaliforniaUSA

Glioblastoma(GBM),one of the most common primary intracranial malignant tumours,is very difficult to be completely excised by surgery due to its irregular ***,we use an MRI/NIR fluorescence dual-modal imaging nanoprobe that includes superparamagnetic iron oxide nanoparticles(SPIONs)modified with indocyanine(Cy7)molecules and peptides(ANG orDANG)to locate malignant gliomas and guide accurate *** peptides/Cy7-SPIONs probes displayed excellent tumour-homing properties and barrier penetrating abilities in vitro.

关键词： tumour malignant surgery

Effect of Beam Current and Diameter on Electron Probe Microanalysis of Carbonate Minerals

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Journal of Earth Science 2019年第4期30卷 834-842页

作者： Xing Zhang Shuiyuan Yang He Zhao Shaoyong Jiang ruoxi Zhang Jing xie State Key Laboratory of Geological Processes and Mineral Resources China University of GeosciencesWuhan 430074China College of Earth Sciences Chengdu University of Technology Chengdu 610059China Faculty of Earth Resources and Collaborative Innovation Center for Scarce and Strategic Mineral Resources China University of GeosciencesWuhan 430074China State Key Laboratory for Mineral Deposits Research School of Earth Sciences and Engineering Nanjing UniversityNanjing 210093China Institute of Tibetan Plateau Research Chinese Academy of Sciences y Beijing 100085China

The effect of operating conditions on the time-dependent X-ray intensity variation is of great importance for the optimal EPMA conditions for accurate determinations of various elements in carbonate minerals. Beam diameters of 0, 1, 2, 5, 10, 15, and 20 μm, and beam currents of 3, 5, 10, 20,and 50 nA were tested. Ca, Mg, Zn, and Sr were found to be more sensitive to electron beam irradiation as compared to other elements, and small currents and large beam diameters minimized the timedependent X-ray intensity variations. We determined the optimal EPMA operating conditions for elements in carbonate: 10 μm and 5 nA for calcite;10 μm and 10 nA for dolomite;5 μm and 10 nA or 10 μm and 20 nA for strontianite;and 20 nA and 5 μm for other carbonate. Elements sensitive to electron beam irradiation should be determined first. In addition, silicate minerals are preferred as standards rather than carbonate minerals.

关键词： carbonate minerals:electron probe microanalysis characteristic X-ray time-dependent intensity beam current beam diameter

益气活血开窍组分中药对脑缺血后白质保护的网络药理学研究及药效学验证

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湖南中医药大学学报 2022年第6期42卷 941-949页

作者：朱秋燕曾鑫冰杨筱倩谢若曦李笑黄小平邓常清湖南中医药大学血管生物学实验室湖南长沙410208 湖南中医药大学中西医结合心脑疾病防治湖南省重点实验室湖南长沙410208 湖南中医药大学医学院湖南长沙410208

目的采用网络药理学方法,以髓鞘再生的主要功能细胞少突胶质前体细胞(oligodendrocyte precursor cells,OPCs)为靶点,预测益气活血开窍组分中药有效成分对脑缺血后白质损伤的促修复作用。方法从TCMSP、PubChem、SwissTargetPrediction... 详细信息

目的采用网络药理学方法,以髓鞘再生的主要功能细胞少突胶质前体细胞(oligodendrocyte precursor cells,OPCs)为靶点,预测益气活血开窍组分中药有效成分对脑缺血后白质损伤的促修复作用。方法从TCMSP、PubChem、SwissTargetPrediction数据库中预测组分中药主要有效成分促进脑卒中后OPCs修复脑白质的作用靶点;整合GEO、GeneCards和CTD数据库中获得的脑卒中疾病靶基因,通过STRING绘制“中药组分-疾病”靶点PPI网络图;并进行GO及KEGG富集分析。同时,采用大鼠脑缺血再灌注模型,从神经功能和形态学评价益气活血开窍组分中药对脑缺血再灌注后脑白质损伤的促修复作用。结果网络药理学共获取33个疾病-药物共同潜在靶点。PPI网络及拓扑分析结果显示VEGFA、PTGS2、NTRK1、ESR2和MET等可能为核心作用靶点,富集分析主要涉及钙离子、PI3K-Akt、VEGF、Rap1、Ras、EGFR酪氨酸激酶抑制剂抗性和MAPK信号通路,KEGG富集分析GEO数据集差异基因结果显示,卒中5 d和15 d均涉及PI3K-Akt信号通路。药理学实验结果显示,益气活血开窍组分中药可改善脑缺血再灌注后大鼠神经功能障碍,减轻脑白质损伤和大鼠胼胝体、纹状体髓鞘脱失。结论益气活血开窍组分中药可改善脑缺血再灌注后白质损伤,机制可能与各有效成分通过多靶点、多途径促进髓鞘再生和白质修复有关。

关键词：网络药理学益气活血开窍组分中药脑缺血再灌注髓鞘再生

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化痰通络汤对脑缺血/再灌注损伤大鼠肠道菌群的影响

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湖南中医药大学学报 2023年第6期43卷 982-991页

作者：曾鑫冰唐三谢若曦贺骊文邓常清杨筱倩黄小平湖南中医药大学血管生物学实验室湖南长沙410208 湖南中医药大学中西医结合心脑疾病防治湖南省重点实验室湖南长沙410208 湖南中医药大学中西医结合病原生物学湖南省重点实验室湖南长沙410208

目的从肠道菌群探讨化痰通络汤抗脑缺血/再灌注(ischemia/reperfusion,I/R)损伤的作用机制。方法将30只SPF级雄性大鼠随机分为假手术组、模型组及化痰通络汤高剂量组(28.66 g/kg)、化痰通络汤中剂量组(14.33 g/kg)、化痰通络汤低剂量组(... 详细信息

目的从肠道菌群探讨化痰通络汤抗脑缺血/再灌注(ischemia/reperfusion,I/R)损伤的作用机制。方法将30只SPF级雄性大鼠随机分为假手术组、模型组及化痰通络汤高剂量组(28.66 g/kg)、化痰通络汤中剂量组(14.33 g/kg)、化痰通络汤低剂量组(7.16 g/kg)、阳性对照组(依达拉奉4 g/kg+丁酸梭菌5.0×108 cfu/mL),每组5只。采用大鼠局灶性脑I/R模型,给药组在造模前2 h灌胃给药,缺血2 h后进行再灌注,于再灌注后24 h后取脑组织和新鲜结肠粪便,氯化三苯四氮唑(triphenyl tetrazolium chloride,TTC)染色法测定脑梗死体积,16S rRNA测序检测结肠内容物肠道菌群变化。结果TTC染色显示,脑I/R后大鼠出现脑梗死灶,与模型组比较,化痰通络汤高、中、低剂量组均能显著减轻脑梗死体积,减少梗死率,且效应具有剂量依赖性(P<0.05,P<0.01)。Alpha和Beta多样性分析显示,与假手术组比较,模型组大鼠肠道菌群发生显著变化(P<0.05),与模型组比较,化痰通络汤中、高剂量组能提高大鼠肠道菌群丰富度和多样性,并能正向调节脑I/R损伤大鼠肠道菌群和优势菌种(P<0.05)。结论化痰通络汤可能通过改善肠道菌群失调、调节肠道微生物种类和数量发挥对损伤大鼠的脑保护作用。

关键词：脑缺血再灌注损伤脑梗死化痰通络汤肠道菌群 16S rRNA

Cucurbitacin B-induced G2/M cell cycle arrest of conjunctival melanoma cells mediated by GRP78-FOXM1-KIF20A pathway

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Acta Pharmaceutica Sinica B 2022年第10期12卷 3861-3876页

作者： Jinlian Wei Xin Chen Yongyun Li ruoxi Li Keting Bao Liang Liao Yuqing xie Tiannuo Yang Jin Zhu Fei Mao Shuaishuai Ni Renbing Jia Xiaofang Xu Jian Li State Key Laboratory of Bioreactor Engineering Shanghai Frontiers Science Center of Optogenetic Techniques for Cell MetabolismFrontiers Science Center for Materiobiology and Dynamic ChemistryShanghai Key Laboratory of New Drug DesignSchool of PharmacyEast China University of Science and TechnologyShanghai 200237China Department of Ophthalmology Ninth People’s HospitalShanghai Jiao Tong University School of MedicineShanghai 200025China Cancer Institute Longhua HospitalShanghai University of Traditional Chinese MedicineShanghai 200032China Yunnan Key Laboratory of Screening and Research on Anti-pathogenic Plant Resources from West Yunnan College of PharmacyDali UniversityDali 671000China Clinical Medicine Scientific and Technical Innovation Center Shanghai Tenth People’s HospitalTongji University School of MedicineShanghai 200092China Key Laboratory of Tropical Biological Resources of Ministry of Education College of PharmacyHainan UniversityHaikou 570228China

Conjunctival melanoma(CM) is a rare and fatal malignant eye tumor. In this study, we deciphered a novel anti-CM mechanism of a natural tetracyclic compound named as cucurbitacin B(CuB). We found that CuB remarkably inhibited the proliferation of CM cells including CM-AS16,CRMM1, CRMM2 and CM2005.1, without toxicity to normal cells. CuB can also induce CM cells G2/M cell cycle arrest. RNA-seq screening identified KIF20A, a key downstream effector of FOXM1 pathway, was abolished by CuB treatment. Further target identification by activity-based protein profiling chemoproteomic approach revealed that GRP78 is a potential target of CuB. Several lines of evidence demonstrated that CuB interacted with GRP78 and bound with a Kdvalue of0.11 μmol/L. Furthermore, ATPase activity evaluation showed that CuB suppressed GRP78 both in human recombinant GRP78 protein and cellular lysates. Knockdown of the GRP78 gene significantly induced the downregulation of FOXM1 and related pathway proteins including KIF20A, underlying an interesting therapeutic perspective. Finally, CuB significantly inhibited tumor progression in NCG mice without causing obvious side effects in vivo. Taken together, our current work proved that GRP78-FOXM1-KIF20A as a promising pathway for CM therapy, and the traditional medicine CuB as a candidate drug to hinder this pathway.

关键词： Conjunctival melanoma Cucurbitacin B Activity-based protein profiling G2/M cell cycle GRP78 FOXM1 KIF20A Rare tumor

Gene expression signatures for phlegm-dampness constitution of Chinese Medicine

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Science China(Life Sciences) 2017年第1期60卷 105-107页

作者： Lingru Li Juan Feng Haiqiang Yao Lan xie Yu Chen Lingling Yang Shujuan Hou Shipeng Zhao Ranran Sun Yanling Wu Tongtong Bai Yingshuai Li ruoxi Yu Ji Wang Qi Wang Center for studies in Constitution Research of Traditional Chinese Medicine Beijing University of Chinese MedicineBeijing 100029China Medical Systems Biology Research Center School of MedicineTsinghua UniversityBeijing 100084China Nutrition Department China-Japan Friendship HospitalBeijing 100029China Preventive Treatment of Disease Department Zhengzhou Hospital of Traditional Chinese MedicineZhengzhou 450002China

Dear Editor, The term ＂individualized medicine＂ was first used in Bio- chemistry Individuality in 1956 by Williams （Williams et al., 1956）. With the emergence of the post-genome era in the 21 st century, individualized precision medicine has attracted increasing attention. In traditional Chinese medicine （TCM）, there is also concept of individualized medicine, which is known as the Chinese constitutional theory, proposed in the 1970s （Wang, 2012）. According to this theory, the human population is divided into nine constitutions, including one balanced constitution （Normality） and eight unbalanced constitutions （Yang-deficient, Yin-deficient, Qi-deficient,

关键词：中医学体质基因表达特征后基因组时代构成要件不平衡 Bio

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植被异质性样区真实性检验的优化采样策略

农业工程学报 2021年第8期37卷 177-186页

作者：李若溪周翔吕婷婷陶醉王锦谢富泰中国科学院空天信息创新研究院北京100101 中国科学院大学北京100049 中国科学院大学电子电气与通信工程学院北京100049

遥感反演植被产品的真实性检验是推动其在农业领域应用水平提升的重要保证,其中异质性植被样区的优化采样设计是真实性检验地面测量过程中的关键技术。该研究以遥感影像作为先验知识,通过K-means聚类分层选取初始样点,利用空间模拟退火... 详细信息

遥感反演植被产品的真实性检验是推动其在农业领域应用水平提升的重要保证,其中异质性植被样区的优化采样设计是真实性检验地面测量过程中的关键技术。该研究以遥感影像作为先验知识,通过K-means聚类分层选取初始样点,利用空间模拟退火算法规划最优采样方案,并采用同期地面实测数据进行检验。研究结果表明,空间模拟退火算法在样点与总体空间变异性的一致性、插值面的精度、插值点和实测点的相关性3个方面都明显优于传统采样方案,2块样区优化后的采样方案插值面与影像面的均方根误差分别为3.1026和2.9627,插值点与实测点的皮尔逊相关系数分别为0.601和0.757,表明空间模拟退火算法可以为真实性检验地面试验提供可靠的优化采样策略。

关键词：遥感算法采样真实性检验植被异质性样区

电子探针多道波谱仪同时测试同一个元素的方法:以石英中Al和Ti含量的测试为例

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高校地质学报 2021年第3期27卷 340-348页

作者：崔继强郭晟彬张若曦谢静杨水源中国地质大学(武汉)地质过程与矿产资源国家重点实验室武汉430074 中国科学院青藏高原研究所大陆碰撞与高原隆升重点实验室北京100101

电子探针显微分析是常用的原位分析矿物中各种主量元素含量的技术方法,但是对于微量元素的分析,提高测试精确度和准确度长期以来面临着各种挑战。常用的提高精确度方法有改变加速电压、加大电流和增加计数时间,但是这些方法容易使样品... 详细信息

电子探针显微分析是常用的原位分析矿物中各种主量元素含量的技术方法,但是对于微量元素的分析,提高测试精确度和准确度长期以来面临着各种挑战。常用的提高精确度方法有改变加速电压、加大电流和增加计数时间,但是这些方法容易使样品遭到破坏,发生元素迁移从而造成测试结果的不准确,增加计数时间还会降低测试效率。文章提出了一种利用电子探针多道波谱仪同时测试同一个元素的方法来提高待测元素特征X射线的强度值。该方法利用电子探针不同波谱仪同时测试同一个待测元素,通过测试标准样品中待测元素的总净计数和待测样品中待测元素的总净计数,结合标准样品中待测元素的浓度计算待测样品中待测元素的含量。该方法在同等测试条件下可以有效的提高待测元素特征X射线的强度值,从而提高了测试的精确度,降低了测试的检测限。文章以石英中Al和Ti含量的测试为例,验证了多道波谱仪同时测试同一个元素的方法在微量元素测试中的应用。测试Al元素时使用一个TAP晶体和一个TAPL晶体,测试Ti元素时使用三个PETL晶体,结合多点背景测试法准确测试了石英中Al和Ti的含量,在20 kV电压、500 nA束流和20μm束斑直径的条件下可将Al的检测限降低至2.6×10^(-6)(3σ),将Ti的检测限降低至2.1×10^(-6)(3σ),测试获得石英参考标样中Al和Ti的含量分别为163.8×10^(-6)±5.8×10^(-6)(3σ)和56.5×10^(-6)±2.0×10^(-6)(3σ),皆在参考值区间(Al:154×10^(-6)±15×10^(-6);Ti:57×10^(-6)±4×10^(-6))内,并且具有较好的长期稳定性。

关键词：电子探针微量元素多道波谱仪同时测试石英

Allele-defined genome reveals biallelic differentiation during cassava evolution

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Molecular Plant 2021年第6期14卷 851-854页

作者： Wei Hu Changmian Ji Haitao shi Zhe Liang Zehong Ding Jianqiu Ye Wenjun Ou Gang Zhou Weiwei Tie Yan Yan Jinghao Yang Xiaoying Yang Yunxie Wei Zhiqiang Jin Jianghui xie Ming Peng Wenquan Wang Anping Guo Biyu Xu Jianchun Guo Songbi Chen Liming Ma Mingcheng Wang Yang Zhou Xiaolong Li ruoxi Li Siyi Guo Xinhui Xiao Zhongqing Wan Feifei An Jie Zhang Qingyun Leng Yin Li Ray Ming Kaimian Li Sanya Research Institute of Chinese Academy of Tropical Agricultural Sciences SanyaHainanChina Hainan Key Laboratory for Biosafety Monitoring and Molecular Breeding in Off-Season Reproduction Regions Key Laboratory of Biology and Genetic Resources of Tropical CropsInstitute of Tropical Bioscience and BiotechnologyChinese Academy of Tropical Agricultural SciencesHaikouHainanChina Hainan Key Laboratory for Protection and Utilization of Tropical Bioresources Hainan Institute for Tropical Agricultural ResourcesChinese Academy of Tropical Agricultural SciencesHaikouHainanChina Biomarker Technologies Corporation BeijingChina Hainan Key Laboratory for Sustainable Utilization of Tropical Bioresources College of Tropical CropsHainan UniversityHaikouHainanChina Biotechnology Research Institute Chinese Academy of Agricultural SciencesBeijingChina Tropical Crops Genetic Resources Institute Chinese Academy of Tropical Agricultural SciencesHaikouHainanChina College of Food Science and Technology Qilu University of Technology(Shandong Academy of Sciences)JinanChina Fu Foundation School of Engineering and Applied Science Columbia UniversityNew YorkUSA State Key Laboratory of Crop Stress Adaptation and Improvement School of Life SciencesHenan UniversityKaifengChina The Genetic Engineering International Cooperation Base of Chinese Ministry of Science and Technology the Key Laboratory of Molecular Biophysics of Chinese Ministry of EducationCollege of Life Science and TechnologyHuazhong University of Science&TechnologyWuhanChina FAFU and UIUC-SIB Joint Center for Genomics and Biotechnology Fujian Provincial Key Laboratory of Haixia Applied Plant Systems BiologyKey Laboratory of GeneticsBreeding and Multiple Utilization of CropsMinistry of EducationFujian Agriculture and Forestry UniversityFuzhouChina Department of Plant Biology University of Illinois at Urbana-ChampaignUrbanaILUSA

Dear Editor,Generation of heterozygous genomes by hybridization between or within species can help maintain plant diversity and serve as a potential source of new species(Baek et al.,2018).Moreover,genomic heterozygosity is associated with genomic coadaptation,developmental stability,and *** definition of alleles in haplotypes is necessary to precisely characterize allelic variation controlling agriculturally important traits(Shi et al.,2019).Currently,most released genomes have mosaic assembly of haplotypes due to random selection or collapse of alleles during genome assembly(Shi et al.,2019),which masked allelic variation and functional differentiation of divergent alleles in heterozygous species.

关键词： maintain evolution assembly

BoBs技术在复杂先天性心脏病遗传学检测和病因研究中的应用

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昆明理工大学学报（自然科学版） 2021年第6期46卷 75-83页

作者：樊若溪王瑜谢明星杨必成朱宝生云南省第一人民医院儿科云南昆明650032 国家卫生健康委员会西部优生重点实验室云南昆明650032 湖北医药学院附属襄阳市第一人民医院湖北襄阳441000 华中科技大学同济医学院附属协和医院湖北武汉430022

探讨细菌人工染色体标记微球阵列技术(BoBs)在揭示不明原因的复杂先天性心脏病遗传学病因中的应用价值,建立针对常见染色体微缺失/微重复综合征的快速临床检测技术策略.共采集了2014年4月-2018年11月间经超声心动图检查临床确诊的复杂... 详细信息

探讨细菌人工染色体标记微球阵列技术(BoBs)在揭示不明原因的复杂先天性心脏病遗传学病因中的应用价值,建立针对常见染色体微缺失/微重复综合征的快速临床检测技术策略.共采集了2014年4月-2018年11月间经超声心动图检查临床确诊的复杂先心病病例样本77例,使用产前BoBs技术分别检测患者在13、18、21、X和Y染色体的非整倍体变异,以及9个区域的微重复与微缺失变异,结果共检出染色体异常的阳性标本17例.其中染色体数目异常11例,包括13三体综合征1例,18三体综合征5例,21三体综合征3例,性染色体异常2例;以及染色体微缺失/微重复综合征6例,包括Miller-Dieker综合征合并Williams-Beuren综合征1例,Langer-Giedion综合征2例,DiGeorge微缺失综合征3例.产前BoBs技术作为一项高通量检测技术,用于特异性检测复杂先心病患者在13、18、21、X、Y染色体非整倍体变异和9个综合征区域的微重复与微缺失,具有获取结果快速、准确、简便的显著优势,为阐明复杂先心病的发病机制和临床干预提供了有效手段.

关键词：先天性心脏病 BACs-on-Beads技术染色体异常微缺失综合征微重复综合征

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