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Genome-wide identification and characterization of basic helix-loop-helix transcription factors in Spodoptera litura upon pathogen infection

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Insect Science 2022年第4期29卷 977-992页

作者： Jie zhang Mengyao Zhu Qilin Li Ting Tang Liang Wen Jielai Zhong ruonan zhang Xiao-Qiang Yu Yuzhen Lu Guangdong Provincial Key Laboratory of Insect Developmental Biology and Applied Technology Institute of Insect Science and TechnologySchool of Life SciencesSouth China Normal UniversityGuangzhouChina Guangzhou Key Laboratory of Insect Development Regulation and Application Research Institute of Insect Science and TechnologySchool of Life SciencesSouth China Normal UniversityGuangzhouChina

Basic helix-loop-helix(bHLH)transcription factors play an important role in a wide range of metabolic and developmental processes in eukaryotes,and bHLH proteins also participate in immune responses,especially in ***,their roles in insects upon entomopathogen infection are *** this study,54 bHLH genes in 41 families were identified in a polyphagous pest,Spodoptera litura,including a new bHLH gene in group B,which is specifically present in Lepidoptera and was thus named *** conserved amino acids in the bHLH domain,structural architecture,and chromosomal distribution of bHLH genes in *** were *** bHLH genes in Plutella xylostella and Apis mellifera were also updated,and genome-wide comparison and phylogenetic analysis of bHLH members in 5 holometabolous insects were *** expression profiles of *** bHLH(SlbHLH)genes in 3 tissues at different developmental stages and their responses to *** nucleopolyhedrovirus(SpltNPV),Nomuraea rileyi(Nr),and Bacillus thuringiensis(Bt)infection were *** SlbHLHs in group B were expressed and differentially expressed during pathogen infections,and SlbHLHs tended to be downregulated in the midgut of *** larvae after *** *** study provides an overview of bHLH family members in *** and their responses to different pathogens used for pest *** findings on bHLH members may contribute to uncovering the mechanism of host-pathogen interaction.

关键词： basic helix-loop-helix entomopathogen infection pest biocontrol Spodoptera liura

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点翠工艺材料替换及其在皮革配饰领域的应用探索

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皮革科学与工程 2024年第5期34卷 92-98页

作者：姚云鹤张若男方束元驰濮瑞凝唐欣雨钟银银四川大学制革清洁技术国家工程实验室四川成都610065 四川大学皮革化学与工程教育部重点实验室四川成都610065 四川大学轻工科学与工程学院四川成都610065 四川大学艺术学院四川成都610065 四川大学建筑与环境学院四川成都610065 四川大学水利水电学院四川成都610065

点翠工艺是极具中华文化意蕴的非物质文化遗产,在古代高等级服饰中占有重要地位,但因其所用的翠羽材料不符合时代要求而濒临窘境。文章通过多种天然羽毛材料及纺织材料的替代试验,结合现代皮革配饰品特点进行设计转化与创新性应用。并... 详细信息

点翠工艺是极具中华文化意蕴的非物质文化遗产,在古代高等级服饰中占有重要地位,但因其所用的翠羽材料不符合时代要求而濒临窘境。文章通过多种天然羽毛材料及纺织材料的替代试验,结合现代皮革配饰品特点进行设计转化与创新性应用。并在此基础上提出以消费需求为引擎,彰显产品时尚属性,兼容相关非遗文化元素,赋予产品故事性等手段,为点翠工艺在皮革制品领域的生产性保护提供思路与借鉴。

关键词：点翠工艺皮革配饰材料设计

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Genome-wide identification and comparative analysis of Cry toxin receptor families in 7 insect species with a focus on Spodoptera litura

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Insect Science 2022年第3期29卷 783-800页

作者： Qilin Li Mengge Li Mengyao Zhu Jielai Zhong Liang Wen Jie zhang ruonan zhang Qiang Gao Xiao-Qiang Yu Yuzhen Lu Guangdong Provincial Key Laboratory of Insect Developmental Biology and Applied Technology Institute of Insect Science and TechnologySchool of Life SciencesSouth China Normal UniversityGuangzhou510631China College of Biology Hunan UniversityChangsha410082China

Cadherin,aminopeptidase N(APN)and alkaline phosphatase(ALP)have been characterized as Cry *** this study,comparative genomic analysis of the 3 receptor families was performed in 7 *** and APNs are divided into three and eight clades in phylogenetic trees,*** in clade 3 and APNs in clade 1 contain multiple paralogs within each species and most paralogs are located closely in *** melanogaster has expanded APNs in clade 5 and were lowly expressed in *** are divided into 16 clades;they may diverge before holometabolous insect speciation except for BtR and Cad89D-like *** insects from different orders containing BtR orthologs are sensitive to Cryl A or Cry3A,while five species without BtR are insensitive to both *** APNs in clade 1,several ALPs in clade 3,BtR and Cad89D-like genes were highly or moderately expressed in larval midgut of Spodoptera litura and the other six species,and several members in these clades have been identi-•fied as Cry *** of putative *** Cry receptors in the midgut after exposing to Bt toxins were also analyzed.

关键词： alkaline phosphatase aminopeptidase N Bacillus thuringiensis cadherin Cry toxin

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基于机器学习的煤矿瓦斯浓度预测技术

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陕西煤炭 2024年第3期43卷 88-91,144页

作者：徐平安张若楠周小雨赵琦琦平安煤炭开采工程技术研究院有限责任公司安徽淮南232000

煤矿发生瓦斯灾害前,往往伴随瓦斯浓度异常,准确判断瓦斯浓度是进行瓦斯突出预测、通风设计等工作的基础。通过机器学习的方法,将影响瓦斯浓度的多种因素进行综合计算,探索一种基于机器学习的多因素煤矿瓦斯浓度预测技术,通过构建模型... 详细信息

煤矿发生瓦斯灾害前,往往伴随瓦斯浓度异常,准确判断瓦斯浓度是进行瓦斯突出预测、通风设计等工作的基础。通过机器学习的方法,将影响瓦斯浓度的多种因素进行综合计算,探索一种基于机器学习的多因素煤矿瓦斯浓度预测技术,通过构建模型、训练模型、实际使用,计算得出预测值与实际值的误差,并结合实际生产矿井数据,进行验证计算,验证此种方法的可行性,将瓦斯预测技术由被动式变为主动式,为煤矿瓦斯浓度预测提供新思路,同时将大数据、深度学习等智能化技术引入至煤矿瓦斯治理中,具有广阔的应用前景。

关键词：机器学习煤矿瓦斯线性回归算法浓度预测

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基于大数据技术的采煤工作面瓦斯抽采数据分析

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陕西煤炭 2024年第4期43卷 66-69页

作者：徐平安张若楠周小雨赵琦琦平安煤炭开采工程技术研究院有限责任公司安徽淮南232000

煤矿采煤工作面在回采过程中,需要对煤层中赋存的瓦斯进行抽采,要先抽后采,保证采煤工作面回采过程中瓦斯治理安全可靠。为减少人工工作量、加强采煤工作面瓦斯治理能力,现引入大数据的技术理念,采用大数据的技术手段,将瓦斯抽采过程中... 详细信息

煤矿采煤工作面在回采过程中,需要对煤层中赋存的瓦斯进行抽采,要先抽后采,保证采煤工作面回采过程中瓦斯治理安全可靠。为减少人工工作量、加强采煤工作面瓦斯治理能力,现引入大数据的技术理念,采用大数据的技术手段,将瓦斯抽采过程中获得的基础数据代入算法模型,得到相应结果。通过对结果数据进行探索分析,获得新的数据分析手段,形成一种新的预警方式;能够在获取基础数据的同时,将需要的计算数据进行实时反馈;减少了日常瓦斯抽采分析中的人工工作量,大大提高了工作效率和安全保障能力。

关键词：瓦斯抽采数据分析数据清洗大数据技术数据展示

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Pd cluster decorated free standing flexible cathode for high performance Li-oxygen batteries

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Nano Research 2024年第4期17卷 2678-2686页

作者： Liang Guo Guoliang zhang ruonan Yang Dongmei zhang Xiuqi zhang Han Yu Xia Li Feng Dang Key Laboratory for Liquid-Solid Structure Evolution and Processing of Materials Ministry of EducationShandong UniversityJinan 250061China

As a promising candidate for the next generation energy storage system,rechargeable lithium-oxygen batteries(LOBs)still face substantial challenges caused by insulating discharge products that preclude their practical *** highly efficient cathode catalysts capable of facilitating formation/decomposition of discharge products is considered as an essential approach towards high performance ***,Pd decorated Te nanowires(Pd@Te NWs)were synthesized as advanced catalyst in LOBs to maximize Pd utilization and achieve synergistic effect,in which Pd clusters were uniformly grown on Te substrate though regulating the Pd:Te ***,Pd@Te nanowires assembled into an interpenetrating network-like structure by vacuum filtration and employed as flexible cathode,enabling LOBs achieved an ultralong 190 cycles stability and a superior specific capacity of 3.35 mAh·cm^(-2).Experimental studies and density functional theory(DFT)calculations reveal the excellent catalytic ability of Pd@Te and synergistic catalytic mechanism of Pd and Te,in which uniform electron distribution,extensive electron exchange,and large adsorption distance between Pd cluster and discharge products promote homogeneous adsorption/desorption of discharge products,while the high adsorption energy of Te substrate for Li species reduces the initial dynamical energy barrier during discharging *** current work provides viable strategy to design composite catalysts for flexible cathode of LOBs with synergistic catalytic effects.

关键词： flexible lithium-oxygen batteries Pd@Te synergistic catalysis cathode catalysts density functional theory(DFT)calculation

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BmMD-2A responds to 20-hydroxyecdysone and regulates Bombyx mori silkworm innate immunity in larva-to-pupa metamorphosis

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Insect Science 2023年第2期30卷 411-424页

作者： ruonan zhang Xue Chen Yuan Wang Xu Bai Qiong Yang Yangjin Zhong Xiao-Qiang Yu Fengliang Jin Wanying Yang Guangdong Laboratory for Lingnan Modern Agriculture Laboratory of Insect Molecular Biology and BiotechnologyGuangdong Provincial Key Laboratory of Agro-animal Genomics and Molecular BreedingCollege of Animal ScienceSouth China Agricultural UniversityGuangzhou510642China Guangdong Laboratory of Bio-Pesticide Creation and Application of Guangdong Province College of Plant ProtectionSouth China Agricultural UniversityGuangzhou510642China The Sericultural and Agri-Food Research Institute of the Guangdong Academy of Agricultural Sciences GuangzhouChina Guangdong Provincial Key Laboratory of Insect Developmental Biology and Applied Technology Institute of Insect Science and TechnologySchool of Life SciencesSouth China Normal UniversityGuangzhouChina

20E-hydroxyecdysone(20E)plays important roles in larval molting and metamorphosis in insects and is also involved in the insect innate immune *** metamorphosis is a highly successful strategy for environmental adaptation and is the most vulnerable stage during which the insect is susceptible to various pathogens.20E regulates a series of antimicrobial peptides(AMPs)through the immunodeficiency(IMD)pathway activation in Drosophila;nevertheless,whether other immune pathways are involved in 20E-regulated insect immunity is *** previous studies showed that BmMD-2A is a member of the MD-2-related lipid recognition(ML)family of proteins that are involved in the Bombyx mori innate immunity Toll signaling *** this study,we further demonstrate that BmMD-2A is also positively regulated by 20E,and the BmMD-2A neutralization experiment suggested that 20E activates some downstream immune effect factors,the AMP genes against Escherichia coli and Staphylococcus aureus,through the regulation of BmMD-2A in larval metamorphosis,implying that *** may use the Toll-ML signaling pathway to maintain innate immune balance in the larval-pupal metamorphosis stage,which is a different innate immunity pathway regulated by 20E compared to the IMD pathway in Drosophila.

关键词： AMPs BmMD-2A 20E innate immune metamorphosis

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Syntheses of new di-nuclear and tetra-nuclear lanthanide 9-anthracenecarboxylates:Magnetocaloric effect,slow magnetic relaxation and luminescent properties

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Journal of Rare Earths 2024年第6期42卷 1101-1109,I0004页

作者： Xing Liu ruonan Yang Lu zhang Jian Zhou Chunmei Huang Huahong Zou Zunhong Xiao Chongqing Key Laboratory of Inorganic Functional Materials College of ChemistryChongqing Normal UniversityChongqing401331China Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources(Ministry of Education of China) School of Chemistry and Chemical Engineering of Guangxi Normal UniversityGuilin541004China School of Chemistry and Materials Guizhou Normal UniversityGuiyang550025China

Lanthanide complex-based materials with magnetic and luminescent properties have received a great deal of attention,owing to their importantly potential applications in diverse *** this work,the 9-anthracenecarboxylic acid(9-Haca)as bridging ligand was applied in combination with optically activeπ-conjugated 2,4,6-tri(2-pyridyl)-1,3,5-triazine(tptz)to construct a series of new di-nuclear and tetranuclear lanthanide 9-anthracenecarboxylates[La_(2)(9-aca)_(6)(tptz)_(2)(μ-H_(2)O)]·0.25H_(2)O(1),[Ln_(2)(9-aca)_(6)(tptz)_(2)(H_(2)O)_(2)]·0.5AQ·CH_(3)COOH_(2)H_(2)O[Ln=Sm(2a),Eu(2b),Gd(2c),Tb(2d),Dy(2e),Tm(2f);AQ=9,10-anthracenedione]and[Ln_(4)(9-aca)_(10)(tptz)_(2)(CH3COO)2]·H_(2)O[Ln=Eu(3a),Gd(3b),Dy(3c)].1 displays a di-nuclear centrosymmetric moiety[La_(2)(9-aca)_(6)(tptz)_(2)(μ-H_(2)O)]built up from two complex[La(9-aca)_(2)(tptz)]groups bridged by one H_(2)O molecule and two syn-syn-μ2-η1:η1-9-aca-bridging ligands,while 2a-2f are isostructural and co mprise another di-nuclear centrosymmetric moieties[Ln_(2)(9-aca)_(6)(tptz)_(2)(H_(2)O)_(2)]based on two complex[Ln(9-aca)_(2)(tptz)(H_(2)O)]groups connected by two syn-syn-μ2-η1:η1-9-aca-bridging ligands,3a-3c are isostructural and show tetra-nuclear centro symmetric moieties[Ln4(9-aca)10(tptz)2(CH3COO)2]containing three types of coordination modes of *** present compounds offer the only examples of lanthanide 9-anthracenecarboxylates decorated by optically active *** investigations of 2a,2c-2f,and 3b-3c indicate that 3c is typical of slow relaxation of the magnetization,while 2c and 3b show significant magnetocaloric effect(MCE)with the value of-ΔSm of 5.26 J/(kg·K)at 4 K and 15.65 J/(kg·K)at 2 K(ΔH=7 T),*** luminescent properties were also studied and reveal that the characteristic luminescent properties of the 7r-conjugated aromatic ligands are introduced into magnetic lanthanide 9-anthracenecarboxylates,simultaneously exhibiting slow magnetic relaxation(or MCE)and luminescen

关键词： Lanthanide complexes Luminescence Magnetic properties Magnetocaloric effect Slow magnetic relaxation Rare earths

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基于大数据技术的智慧机场管理平台的设计与研究

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移动信息 2024年第3期46卷 194-196页

作者：张若楠成都双流国际机场股份有限公司成都610000

为提高机场运营效率、提升服务质量和保障航班安全,智慧机场管理平台应运而生。基于大数据技术的智慧机场管理平台利用先进的数据分析和处理技术,能实时监控和管理机场各项运营指标,实现智能化的运营决策和资源调配。文中旨在探讨基于... 详细信息

为提高机场运营效率、提升服务质量和保障航班安全,智慧机场管理平台应运而生。基于大数据技术的智慧机场管理平台利用先进的数据分析和处理技术,能实时监控和管理机场各项运营指标,实现智能化的运营决策和资源调配。文中旨在探讨基于大数据技术的智慧机场管理平台的设计与研究,通过对机场运营数据的采集、存储、分析和应用,提出了一种高效、智能的机场管理模式,以适应日益复杂的机场运营环境。

关键词：大数据技术智慧机场管理平台设计与研究

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The modulatory effect of oolong tea polyphenols on intestinal flora and hypothalamus gene expression in a circadian rhythm disturbance mouse model

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Food Science and Human Wellness 2024年第2期13卷 748-764页

作者： ruonan Yan Chi-Tang Ho Yanan Liu Shengnan Zhan Zufang Wu Xin zhang Department of Food Science and Engineering Ningbo UniversityNingbo 315211China Department of Food Science Rutgers UniversityNew BrunswickNew Jersey 08901USA

The interaction between host circadian rhythm and gut microbes through the gut-brain axis provides new clues for tea polyphenols to improve host *** present research showed that oolong tea polyphenols(OTP)improved the structural disorder of the intestinal flora caused by continuous darkness,thereby modulating the production of metabolites related to pyruvate metabolism,glycolysis/gluconeogenesis,and tryptophan metabolism to alleviate the steady-state *** fecal microbiota transplantation from the OTP group,the single-cell transcriptomic analysis revealed that OTP significantly increased the number of hypothalamus cell clusters,up-regulated the number of astrocytes and fibroblasts,and enhanced the expression of circadian rhythm genes Cry2,Per3,Bhlhe41,Nr1d1,Nr1d2,Dbp and Rorb in hypothalamic *** results confirmed that OTP can actively improve the intestinal environmental state as well as internal/peripheral circadian rhythm disorders and cognitive impairment,with potential prebiotic functional characteristics to notably contribute to host health.

关键词： Gut microbiota Circadian rhythm Oolong tea polyphenols Single-cell sequencing Metabolism

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