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Genome-wide CRISPR screening reveals key genes and pathways associated with 20-hydroxyecdysone signal transduction in the silkworm(Bombyx mori)

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Insect Science 2024年第1期31卷 47-58页

作者： Hao Sun Jingya Chen ruolin wang Dan Liu Na Zhang Tong Zhang Ling Jia Sanyuan Ma Qingyou Xia State Key Laboratory of Silkworm Genome Biology Southwest UniversityChongqingChina Biological Science Research Center Southwest UniversityChongqingChina

Metamorphosis is a complex developmental process involving multiple pathways and a large number of genes that are regulated by juvenile hormone(JH)and 20-hydroxyecdysone(20E).Despite important progress in understanding various aspects of silkworm biology,the hormone signaling pathway in the silkworm remains poorly understood.Genome-wide screening using clustered regularly interspaced short palindromic repeats(CRISPR)/CRISPR-associated protein 9(Cas9)-based libraries has recently emerged as a novel method for analyzing genome function,enabling further research into essential genes,drug targets,and virus-host interaction.Previously,we constructed a genome-wide CRISPR/Cas9-based library of the silkworm(Bombyx mori)and successfully revealed the genes involved in biotic or abiotic stress factor responses.In this study,we used our silkworm CRISPR library and large-scale genome-wide screening to analyze the key genes in the silkworm 20E signaling pathway and their mechanisms of action.Functional annotation showed that 20E regulates key proteins in processes that mainly occur in the cytoplasm and nucleus.Pathway enrichment analysis showed that 20E can activate phosphorylation and may affect innate immunity,interfere with intracellular nutrition and energy metabolism,and eventually cause cell apoptosis.The screening results were experimentally validated by generating cells with knockout alleles of the relevant genes,which had increased tolerance to 20E.Our findings provide a panoramic overview of signaling in response to 20E in the silkworm,underscoring the utility of genome-wide CRISPR mutant libraries in deciphering hormone signaling pathways and the mechanisms that regulate metamorphosis in insects.

关键词： CRISPR/Cas9 CRISPR library screening ecdysone 20E signal transduction silkworm whole genome editing

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The impact of water quality on the formation of halogenated benzoquinones and the adsorption efficiency by activated carbon

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Journal of Environmental Sciences 2024年第1期135卷 693-702页

作者： Yongjing wang Song Gao Xinyu Yan Songtao wang ruolin Zhang Yan Zhou Lianhai Ren Cheng Li State Environmental Protection Key Laboratory of Food Chain Pollution Control Beijing Technology and Business UniversityBeijing 100048China Institute of Scientific and Technical Information of China Beijing 100038China Institute of Quality Standard and Testing Technology Beijing Academy of Agriculture and Forestry SciencesBeijing 100097China Key Laboratory of Cleaner Production and Integrated Resource Utilization of China National Light Industry Beijing Technology and Business UniversityBeijing 100048China

Halogenated benzoquinones(HBQs)could cause bladder cancer,but there were few related studies on the generation and control.In this study,the impact of different precursors,pH,bromide concentration,and algae-derived organic matters on the formation of HBQs and the removal efficiency by activated carbon were investigated.It was found that the chlorination of bisphenol A produced the most 2,6-dichloro-1,4-benzoquinone(2,6-DCBQ),reaching 14.86μg/L at 1 hr,followed by tyrosine,2-chlorophenol,P-hydroxybenzoic acid,trichlorophenol,and N-methylaniline.The production of 2,6-DCBQ increased first and then decreased from 0 to 36 hr(chlorination doses 0-20 mg/L),indicating that HBQs were unstable in water.Trihalomethanes(THMs)were detected during chlorination,and the concentration increased with prolongation of reaction time.2,6-DCBQ production decreased and 2,6-dibromo-1,4-benzoquinone(2,6-DBBQ)production increased with increment bromide concentration and the bromide promoted the formation of tribromomethane.The production of 2,6-DCBQ decreased with increase of pH,and the maximum production was 141.38μg/L at pH of 5.Microcystis aeruginosa,Chlorella algae cells,and intracellular organic matters(IOM)could be chlorinated as potential precursors for HBQs.The most amount of 2,6-DCBQ was generated from algae cells of Microcystis aeruginosa,followed by Chlorella algae cells,Microcystis aeruginosa IOM,and Chlorella IOM.This study compared the removal efficiency of HBQs by granular activated carbon(GAC)and columnar activated carbon(CAC)under different carbon doses and initial concentrations of HBQs.It was found that the removal efficiency by GAC(80.1%)was higher than that by CAC(51.8%),indicating that GAC has better control for HBQs.

关键词： HBQS DBPS Drinking water Algae derived organic matters Activated carbon

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Micro/nanoelectrode-based electrochemical methodology for single cell and organelle analysis

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Nano Research 2024年第1期17卷 196-206页

作者： Chuchu Xu De Yang Yuchan wang ruolin Liu Fan wang Zhongqun Tian Keke Hu Department of Chemistry College of Chemistry&Chemical EngineeringXiamen UniversityXiamen 361005China

Cells are the basic unit of life.Electrochemical analysis of single cells/organelles is essential for uncovering the molecular mechanisms of physiological and pathological processes that are difficult to elucidate on a larger scale.This paper provides an overview of the commonly used fabrication methods for micro/nanoelectrodes applied in the investigations of single cells/organelles as well as the corresponding electrochemical measurements over the last four years including extracellular measurement,combination of extra and intracellular measurement,intracellular reactive oxygen species and reactive nitrogen species(ROS/RNS)measurement,and isolated organelles measurement.

关键词： single cell/organelle micro/nanoelectrode electrochemical measurement chemical transmitters

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The First Verification Test of Space-Ground Collaborative Intelligence via Cloud-Native Satellites

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China Communications 2024年第4期21卷 208-217页

作者： wang Shangguang Zhang Qiyang Xing ruolin Qi Fei Xu Mengwei State Key Laboratory of Networking and Switching Beijing University of Posts and TelecommunicationsBeijing 100876China Star Network and Intelligent Computing Laboratory Beiyou Shenzhen InstituteShenzhen 518000China Huawei Technologies Co. LtdShenzhen 518129China

Recent advancements in satellite technologies and the declining cost of access to space have led to the emergence of large satellite constellations in Low Earth Orbit(LEO).However,these constellations often rely on bent-pipe architecture,resulting in high communication costs.Existing onboard inference architectures suffer from limitations in terms of low accuracy and inflexibility in the deployment and management of in-orbit applications.To address these challenges,we propose a cloud-native-based satellite design specifically tailored for Earth Observation tasks,enabling diverse computing paradigms.In this work,we present a case study of a satellite-ground collaborative inference system deployed in the Tiansuan constellation,demonstrating a remarkable 50%accuracy improvement and a substantial 90%data reduction.Our work sheds light on in-orbit energy,where in-orbit computing accounts for 17%of the total onboard energy consumption.Our approach represents a significant advancement of cloud-native satellite,aiming to enhance the accuracy of in-orbit computing while simultaneously reducing communication cost.

关键词： cloud-native satellite orbital edge computing satellite inference verification test

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My Bosom Friends From France

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China News Release 2024年第2期 18-21页

作者： Zheng ruolin the China Institute of Fudan University the Taihe Institute

I was often asked how I feel about France now that I left the country after spending over two decades in Paris.My usual responseis"It'salong story."Indeed,the French Republic has long been a country of importance to n... 详细信息

关键词： has France story

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My Bosom Friends From France(Ⅱ)

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中国新闻发布(英文版) 2024年第5期 65-68页

作者： Zheng ruolin

The civilizations of France and China are humanity's most creative treasures,whether viewed realistically or historically.And French friends serve as an important bridge through which we can gain insights into French civilization.I,as a Chinese national,have undeniably gained abundant cultural fruits from the many acquaintances I have made in France throughout my 20-year sojourn there.In my articles,I have shared these fruits with my fellow Chinese.Conversely,I have also relayed objective and positive information about China to my French friends,giving them a picture about the real China.

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Efficient Contact between H_(2)O and N-Coordinate Ru Nanoparticles in Three-Dimensionally Ordered Macro/Mesoporous Carbon Boosting Alkaline HER

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Chinese Journal of Chemistry 2024年第2期42卷 164-170页

作者： Jiaqing Luo Chunyu Liu ruolin Zhang Yuanqing Sun Hua Xu Qingying Hu Manxue Zhang Jun Li Zhenxing Li Weiyu Song Yuechang Wei Jian Liu State Key Laboratory of Heavy Oil and Bejing Key Lab of Oil&Gas Optical Detection Technology China University of PetroleumBeijing 102249China Laboratory of Heavy Oil at Karamay Ching University of Petroleum-Beijing at KaramayKaramayXinjiang 83400China Basic Research Center for Energy Interdisciplinary College of ScienceChina University of PetroleumBeijing 102249China Petrochemical Research Institute PetroChina Company LimitedBeijing 102206China BeijingNationalLaboratoryfor Condensed Matter Physics Institute of PhysicsChinese Academy of SciencesBeijing 100190China

In this study,a novel approach is proposed to achieve the uniformly dispersed Ru nanoparticles with N coordination loaded on three-dimensionally ordered macro/mesoporous carbon(3DOMMC)through simultaneous pyrolysis of Ru^(3+)and cyanamide on 33DOMMC.In an alkaline medium,the synthesized catalysts exhibit exceptional hydrogen evolution reaction(HER)performance.Specificall,Ru-N/3DOMMC demonstrates a significantly low overpotential of 13.8 mV to achieve acrent density of 10 mA.cmzandit exhibits a mass activity 17.5 times higher than that of commercial Pt/C.The outstanding performance could be attributed to the ultrahigh Ru dispersion and more efficient contact between active sites and reactant,which derived from the large specific surface area and interconnective three-dimensionally macro/mesopores of 3DOMMC.

关键词： 3DOMMC Hydrogen evolution reaction Electrocatalysis Solid-liquid contact Energy conversion

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Effectiveness of Inactivated COVID-19 Vaccines Against Symptomatic,Pneumonia,and Severe Disease Caused by the Delta Variant:Real World Study and Evidence—China,2021

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China CDC weekly 2022年第4期4卷 57-65页

作者： Dan Wu Yanyang Zhang Lin Tang Fuzhen wang Ying Ye Chao Ma Hui Zheng Wenzhou Yu Lei Cao Yifan Song Abuduwaili Reyimu Xiaoxiao Zhang Haifeng wang Yifei Nie Mingxia Lu Muge Qi Jun Li ruolin wang Kaichao Yang Changshuang wang Lawrence Everett Rodewald Geroge Fu Gao Zhijie An Zundong Yin Chinese Center for Disease Control and Prevention BeijingChina Henan Provincial Disease Control and Prevention ZhengzhouHenanChina Akesu Prefectural Center for Disease Control and Prevention AkesuXinjiang Uygur Autonomous RegionChina China Field Epidemiology Training Program Chinese Center for Disease Control and PreventionBeijingChina

Summary What is already known about this topic?Effectiveness of China’s 2 inactivated vaccines(BBIBPCorV and CoronaVac)against pre-Delta severe acute respiratory syndrome coronavirus-2(SARS-CoV-2)variants ranged from 47%to over 90%,depending on the clinical endpoint,and with greater effectiveness against more severe coronavirus disease 2019(COVID-19).During an outbreak in Guangdong,inactivated vaccine effectiveness(VE)against the Delta variant was 70%for symptomatic infection and 100%for severe COVID-19.However,separate or combined VE estimates for the two inactivated vaccines against Delta are not available.What is added by this report?In an outbreak that started in a hospital,VEs of completed primary vaccination with inactivated COVID-19 vaccines against symptomatic COVID-19,COVID-19 pneumonia,and severe COVID-19 caused by the Delta variant were 51%,61%,and 82%.Completed primary vaccination reduced the risk of progressing from mild to moderate or severe COVID-19 by 74%.VE estimates for BBIBP-CorV and CoronaVac or combined vaccination were similar,and partial vaccination was ineffective.What are the implications for public health practice?Completed primary vaccination with either of the 2 inactivated COVID-19 vaccines reduces risk of symptomatic COVID-19,COVID-19 pneumonia,and severe COVID-19 caused by the Delta variant.Completion of the completed primary vaccination with two doses is necessary for protection from Delta.

关键词： vaccination doses vaccine

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磁性煤基复合催化剂/H_(2)O_(2)类芬顿体系高效降解喹啉

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煤炭学报 2024年第3期49卷 1657-1668页

作者：梁华根王安虎王圣程若霖陈浮中国矿业大学碳中和研究院江苏徐州221008 中国矿业大学材料与物理学院江苏徐州221008 国家能源集团科学技术研究院有限公司、低碳智能燃煤发电与超净排放全国重点实验室江苏南京210023 河海大学公共管理学院江苏南京211100

为实现煤炭资源利用最大化和煤化工废水中喹啉的高效去除,以煤化程度最低的褐煤为原料,经过酸洗和采用聚乙烯吡咯烷酮进行表面修饰,再通过负载普鲁士蓝类似物(CoFe-PBA)和高温煅烧,制备了CoFe@Coal-C复合材料,用作非均相类芬顿反应催化... 详细信息

为实现煤炭资源利用最大化和煤化工废水中喹啉的高效去除,以煤化程度最低的褐煤为原料,经过酸洗和采用聚乙烯吡咯烷酮进行表面修饰,再通过负载普鲁士蓝类似物(CoFe-PBA)和高温煅烧,制备了CoFe@Coal-C复合材料,用作非均相类芬顿反应催化剂,展现出优异的喹啉降解性能。结果表明,CoFe@Coal-C具有适当的比表面积和孔隙结构,为活性物种提供了传输通道,有利于电子扩散和相互作用。同时,CoFe@Coal-C还表现出典型的超顺磁特性,有利于从水溶液中分离和循环利用。考察了催化剂投加量、初始喹啉质量浓度、溶液pH、及水中共存物质对喹啉降解效率的影响,结果表明,在仅有H_(2)O_(2)存在和仅有催化剂存在的情况下,喹啉的去除率几乎可忽略不计,表明活性氧物种是导致喹啉质量浓度降低的主要因素,而催化剂是催化H_(2)O_(2)产生强氧化性活性氧物种的必要条件。在有H_(2)O_(2)存在、初始喹啉质量浓度为10.0 mg/L、催化剂投加量为0.6 g/L、pH为7的条件下,反应30 min后,CoFe@Coal-C复合材料对喹啉的降解效率达到99.2%。此外,相比纯CoFe合金和纯Coal-C,CoFe@Coal-C对喹啉的降解速率常数分别提高了1个和3个数量级,表明CoFe合金和Coal-C的协同作用是提高催化剂活性的关键。Cl^(-)、HCO_(3)^(-)、SO_(4)^(2-)和H_(2)PO_(4)^(-)等共存阴离子,以及初始pH对CoFe@Coal-C/H_(2)O_(2)催化喹啉降解的性能几乎没有影响。自由基淬灭实验和电子顺磁共振光谱表明·OH是主要活性物种,通过高效液相色谱-质谱联用仪检测了喹啉降解的中间产物,并推测了喹啉降解的可能路径。研究表明磁性CoFe@Coal-C催化材料对洗煤废水中喹啉等有机污染物的去除非常高效,具有广阔的应用前景,为洗煤废水的高效治理和资源循环利用提供科学依据。

关键词：褐煤喹啉类芬顿反应降解磁性材料

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边疆经济高质量发展重点、现实约束与突破路径

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经济纵横 2024年第2期 41-47页

作者：李光辉王若琳对外经济贸易大学国际经济研究院北京100105

解决边疆经济矛盾、突破边疆经济约束、探寻边疆经济新型发展之路,不仅是建设边疆地区、维护国家安全稳定的内在需要,也是第二个百年奋斗目标要求下构建新发展格局、深化区域协调发展和增强国家经济社会发展韧性的必然选择。基于边疆经... 详细信息

解决边疆经济矛盾、突破边疆经济约束、探寻边疆经济新型发展之路,不仅是建设边疆地区、维护国家安全稳定的内在需要,也是第二个百年奋斗目标要求下构建新发展格局、深化区域协调发展和增强国家经济社会发展韧性的必然选择。基于边疆经济的历史实践和现实形势,边疆经济高质量发展的重点集中在创新驱动与边疆创新动能培育、区域经济联动与区域经济一体化、以要素集聚促进经济集聚、沿边高水平开放与融入新发展格局。为突破外部冲击、动能切换、结构失衡和体制机制局囿四重约束,促进边疆经济高质量发展,应推进边疆地区全面协同创新,提升边疆经济综合竞争力;加快边疆地区同其他地区的深度融合,强化区域经济联动式发展;促进优质生产要素集中集聚,加快推进边疆产业转型升级;构建高水平对外开放形态,形成边疆对外开放新格局;完善边疆地区政策体系,全面保障边疆地区的开放发展。

关键词：边疆经济创新驱动区域联动高水平对外开放

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