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Multiplex ratiometric gold nanoprobes based on surface-enhanced Raman scattering enable accurate molecular detection and imaging of bladder cancer

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Nano Research 2022年第4期15卷 3487-3495页

作者： Xiao Liang Pu Zhang Minghai Ma Tao Yang Xiangwei Zhao Rui Zhang Minxuan Jing rundong song Lei Wang Jinhai Fan Department of Urology The First Affiliated HospitalXi’an Jiaotong UniversityXi’an 710061China Department of Thoracic Surgery Tangdu HospitalAir Force Medical UniversityXi’an 710038China Department of Urology Zhejiang Provincial People’s HospitalPeople’s Hospital of Hangzhou Medical CollegeHangzhou 310014China State Key Laboratory of Bioelectronics School of Biological Science and Medical EngineeringNational Demonstration Center for Experimental Biomedical Engineering EducationSoutheast UniversityNanjing 210096China

Recently,surface-enhanced Raman scattering(SERS)has been successfully used in the non-invasive detection of bladder tumor(BCa).The internal standard method was considered as an effective ratiometric strategy for calibrating signal fluctuation originated from the interference of measurement conditions and ***,it is still difficult to detect the target mRNA quantitatively using the current ratiometric SERS *** this study,we developed an internal reference based ratiometric SERS *** kinds of molecular beacons(MB)carrying Raman reporter molecules were anchored on sea-urchinlike Au nanoclusters(AuNCs).Thymidine kinase1(TK1)MBs with hexachlorofluorescein(HEX)were used to capture tumor biomarker TK1 mRNA,and glyceraldehyde 3-phosphate dehydrogenase(GAPDH)MBs with 5(6)-carboxyfluorescein(FAM)were used to offer internal standard *** internal reference GAPDH MB can reflect the consistent content of the GAPDH mRNA in single *** ratiometric method(I745/I645)can more accurately reflect the content of target mRNA in single *** ratiometric nanoprobes had excellent stability(coefficient of variation:0.3%),high sensitivity(detection limit:3.4 pM),high specificity(capable of single-base mismatch recognition)and ribozyme-resistant ***,the nanoprobes can effectively distinguish BCa cells from normal cells,and it was easy to contour the single BCa cell using the ratiometric *** combining asymmetric polymerase chain reaction(PCR)and ratiometric nanoprobes,it was easy to distinguish the SERS ratio(I_(745)/I_(645))as low concentration as 10-14 *** clinical detection in urine samples from patients with BCa confirmed its potential for early noninvasive diagnosis of BCa with the sensitivity of 80%and specificity of 100%,which is superior to the current urine cytological method.

关键词： surface-enhanced Raman scattering(SERS) internal reference bladder cancer non-invasive detection SERS imaging

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R11 peptides can promote the molecular imaging of spherical nucleic acids for bladder cancer margin identification

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Nano Research 2022年第3期15卷 2278-2287页

作者： Minghai Ma Pu Zhang Xiao Liang Daxiang Cui Qiuya Shao Haibao Zhang Mengzhao Zhang Tao Yang Lu Wang Nan Zhang Minxuan Jing Lu Zhang Weichao Dan rundong song Xi Liu Jiatao Hao Yuhang Chen Lijiang Gu Lei Wang Jinhai Fan Department of Urology The First Affiliated HospitalXi’an Jiaotong UniversityXi’an 710061China Department of Urology Zhejiang Provincial People’s HospitalPeople’s Hospital of Hangzhou Medical CollegeHangzhou 310014China Department of Thoracic Surgery Tangdu HospitalAir Force Medical UniversityXi’an 710038China Department of Bio-Nano-Science and Engineering National Key Laboratory of Nano/Micro Fabrication TechnologyInstitute of Micro-Nano Science and TechnologyShanghai Jiao Tong UniversityShanghai 200240China

One of the critical problems in bladder cancer(BC)management is the local recurrence of ***,achieving the accurate delineation of tumor margins intraoperatively remains extremely difficult due to the lack of effective tumor margin recognition ***,survivin molecular beacon(MB)and R11 peptide-linked spherical nucleic acids(SNAs)were synthesized as nanoprobes(AuNP-MB@R11)for sensitive detection of BC *** properties proved that R11 peptides and survivin MB were successfully loaded onto the surface of ***-MB@R11 had good stability against nuclease activity and high sensitivity and specificity to detect survivin single strand DNA(ssDNA)in *** to cytology,R11 peptides could increase the BC targeting ability and membrane penetrability of ***,R11 peptides significantly promoted the disintegration of lysosomes and the release of SNAs to enhance fluorescence imaging *** RNA sequencing proved that some genes and pathways related to endocytosis and lysosomes were significantly regulated,such as AGPAT5,GPD1L,and *** orthotopic BC models and a clinical sample from a patient with BC,AuNP-MB@R11 showed a more legible cancerous fluorescence margin and offered remarkably improved detection compared to those achieved by SNAs.R11 peptide-linked SNAs present promising potential to identify BC margin,which may help to improve the R0 resection rate in surgery and improve patients’quality of life.

关键词： R11 peptides molecular beacons spherical nucleic acids tumor targeting lysosome escape bladder cancer

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产业结构调整背景下的河南省智慧园区建设研究

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创新科技 2019年第2期19卷 73-78页

作者：王金晶陈璇宋润东李展鹏河南省胸科医院河南郑州450003 河南财经政法大学公共管理学院河南郑州450046 郑州航空港兴港智慧城市有限公司河南郑州451162

随着建设智慧城市这一浪潮席卷全球,智慧园区这一概念也愈来愈受到社会各界的关注。本研究以理论上较为完善的智慧园区架构为标准,结合河南省智慧园区建设现状及调研成果,分析河南省智慧园区建设中的不合理之处,针对问题提出科学合理的... 详细信息

随着建设智慧城市这一浪潮席卷全球,智慧园区这一概念也愈来愈受到社会各界的关注。本研究以理论上较为完善的智慧园区架构为标准,结合河南省智慧园区建设现状及调研成果,分析河南省智慧园区建设中的不合理之处,针对问题提出科学合理的对策,以期提高智慧园区建设水平,落实国家信息化发展战略,拉动河南省经济发展。

关键词：智慧园区物联网信息化

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Shallow Genetic Structure of Pholis fangi in Bohai Sea and Yellow Sea Inferred from mt DNA Control Region

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Journal of Ocean University of China 2019年第4期18卷 947-952页

作者： GAO Tianxiang LI Lin FANG rundong LIU Guanghui WANG Lei XU Hanxiang song Na Fishery College Zhejiang Ocean University Zhoushan 316022 China Key Laboratory for Technology Research on Sustainable Utilization of Marine Fishery Resources Zhoushan 316022 China Marine Science and Engineering College Qingdao Agricultural University Qingdao 266109 China The Key Laboratory of Mariculture Ministry of Education Ocean University of China Qingdao 266003 China Oceans and Fisheries Bureau of Dongying District Dongying 257091 China

Pholis fangi is an ecologically important fish species inhabiting Chinese coastal waters of Yellow Sea and Bohai Sea. To investigate the genetic diversity and population genetic structure of P. fangi, a fragment of 487 bp in the first hypervariable region (HVR-1) of the mitochondrial DNA (mtDNA) control region was sequenced for 181 individuals collected from Bohai Sea and Yel- low Sea. A total of 18 polymorphic sites were detected, which defined 25 haplotypes. A moderate level of haplotype diversity (h = 0.7052) and a low level of nucleotide diversity (π= 0.0028) were detected. Both the phylogenetic tree and the haplotype network showed no significant genealogical structure difference among sampling locations. Pairwise FST comparison and hierarchical mo- lecular variance analysis (AMOVA) revealed that no significant genetic structure difference existed throughout the investigated re- gions, suggesting a high gene exchange among different populations. The results of neutrality test and mismatch distribution analysis indicated that a late Pleistocene population expansion (38000 127000 years ago) happened. Seasonal schooling migration may con- tribute to the genetically homogeneous population structure of the species.

关键词： Pholis fangi control region of mtDNA genetic diversity genetic structure demographic history

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Identification,characterization,and verification of miR399 target gene in grape

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Horticultural Plant Journal 2024年第1期10卷 91-102页

作者： Maosong Pei Hainan Liu Tonglu Wei Huiying Jin Yihe Yu Mengting Ma Xiaomeng song rundong Dai Dalong Guo Henan Engineering Technology Research Center of Quality Regulation and Controlling of Horticultural Plants College of Horticulture and Plant ProtectionHenan University of Science and TechnologyLuoyangHenan 471023China

The microRNA miR399 plays an important role in phosphorus signal transduction pathways in ***,miR399 was shown to be closely associated with berry ripening in grape(Vitis vinifera).The objective of the present study was to elucidate the evolutionary characteristics of the miR399 gene family in grape and to verify the cleavage effect on the target *** miR399s were identified by miRNA sequencing and retrieval from the miRBase *** mature sequences and precursor sequences were subjected to phylogenetic analysis to reconstruct evolutionary trees,as well as secondary structure analysis of the precursor sequence,and prediction of target *** cisacting elements in the miR399 promoter were predicted and the cleavage effect of grape miR399b on its target genes was *** grape miR399 family comprised nine precursor sequences and nine mature *** precursor sequences formed a typical and stable stem—loop *** minimum folding free energy ranged from-55.70 kcal·mol^(-1)to-37.40 kcal·mol^(-1).Multiple sequence alignment revealed that the miR399 family was highly *** grape miR399 family was phylogenetically closely related to peach,apple,and citrus *** miR399s were predicted to target inorganic phosphate transporter 1—3,phospholipase D delta-like,and *** cleavage effect of grape miR399b on the target genes was verified by means of a dual-luciferase assay and 5’*** GUS staining showed that the promoter activity of miR399b was promoted by GA3treatment.

关键词： Grape miR399 Phosphorus metabolism Early ripening

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虚拟现实辅助的记忆宫殿法对大学生记忆力的影响

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中国医学教育技术 2021年第5期35卷 620-626页

作者：刘博豪宋润东赵荣辉李启鑫程广浩高嘉蔚 Andrea Gonsalves 李帆胡浩王涛西安交通大学基础医学院西安710061 西安交通大学人文学院西安710061 西安交通大学国际教育学院西安710061

记忆宫殿法是一种历史悠久、用途广泛的记忆技术,但是该法对使用者的空间想象力、创造力以及视觉化能力具有较高要求。该研究以大学生为研究对象,评估采用虚拟现实技术辅助的记忆宫殿法记忆序列词汇的效果。结果发现,虚拟现实辅助的记... 详细信息

记忆宫殿法是一种历史悠久、用途广泛的记忆技术,但是该法对使用者的空间想象力、创造力以及视觉化能力具有较高要求。该研究以大学生为研究对象,评估采用虚拟现实技术辅助的记忆宫殿法记忆序列词汇的效果。结果发现,虚拟现实辅助的记忆宫殿法提升了研究对象记忆序列词汇的成绩,而且采用该法每周训练2次、持续3周,或每周训练3次、持续2周均能使研究对象的记忆能力提升至最佳状态。

关键词：记忆宫殿虚拟现实记忆术

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A sufficient bit rate condition for stabilizing a scalar continuous-time nonlinear system with bounded processing delay

A sufficient bit rate condition for stabilizing a scalar con...

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第37届中国控制会议

作者： rundong Dou Jiayu Chen Feng Li song Wang Qiang Ling Dept.of Automation University of Science and Technology of China

This paper proposes a model-based event-triggered control strategy, which is utilized to achieve the input-to-state stability for a scalar continuous time nonlinear system. Due to the processing delay, the sampled feedback signal cannot be transmitted immediately, i.e., the transmission time instant of the feedback signal is not always equal to its sampling time *** gap between them measures how much information can be carried. A sufficient bit rate condition to stabilize a scalar continuous time nonlinear system is derived. The condition is only determined by the parameter of Lipschitz condition and the bound of processing delay. Compared with the periodic sampling control strategy, much less bits are used by the event-triggered control one for the particular case of stabilizing a scalar continuous time linear system. Simulations are done to verify the effectiveness of the stabilizing bit rate condition.

关键词： Networked control systems Nonlinear system Event-triggered control Input-to-state stability Bit rate condition

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混合定子铁心再制造电机应变数值仿真与转矩不平衡分析

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中国机械工程 2020年第9期31卷 1080-1088页

作者：宋守许胡孟成杜毅罗润东合肥工业大学机械工业绿色设计与制造重点实验室合肥230009 合肥工业大学机械工程学院合肥230009

提出将永磁电机的机壳、端盖、转子等零部件留用,定子铁心更换为由非晶合金叠片和硅钢叠片轴向混合叠压而成的混合定子铁心的永磁电机再制造方式。以一台混合定子铁心再制造电机为对象,分析了电机的空载气隙磁密轴向分布规律;计算了非... 详细信息

提出将永磁电机的机壳、端盖、转子等零部件留用,定子铁心更换为由非晶合金叠片和硅钢叠片轴向混合叠压而成的混合定子铁心的永磁电机再制造方式。以一台混合定子铁心再制造电机为对象,分析了电机的空载气隙磁密轴向分布规律;计算了非晶合金定子和硅钢定子对应的径向力密度,应用电磁机械耦合模型对再制造电机应变进行数值分析;研究了再制造电机单位电磁转矩轴向分布规律,给出了再制造电机电磁转矩计算公式,发现了非晶合金定子和硅钢定子对应电磁转矩不相等引起的转矩不平衡现象,分析了转矩不平衡对转子铁心的影响。制作了再制造电机并进行了振动试验,试验结果证明了分析的正确性。

关键词：混合定子铁心再制造电机电磁转矩转矩不平衡应变数值仿真

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一种采用2级反向传播神经网络的输电线路故障测距方法

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西安交通大学学报 2019年第9期53卷 61-69页

作者：焦在滨宋新尧李炳绪吴润东西安交通大学电气工程学院西安710049 西安交通大学陕西省智能电网重点实验室西安710049 国网安徽省电力有限公司经济技术研究院合肥230022

针对输电线路的故障测距中过渡电阻及电气量测量误差影响测距精度的问题,提出了一种采用2级反向传播(BP)神经网络的输电线路故障测距方法。通过分析双端电气量随过渡电阻的变化情况,确定了双端电气量变化规律的区域特性,提出了基于过渡... 详细信息

针对输电线路的故障测距中过渡电阻及电气量测量误差影响测距精度的问题,提出了一种采用2级反向传播(BP)神经网络的输电线路故障测距方法。通过分析双端电气量随过渡电阻的变化情况,确定了双端电气量变化规律的区域特性,提出了基于过渡电阻分区后在不同区域分别进行精确定位的研究思路。利用第1级网络对双端电气量进行数据融合,将故障场景分为低阻故障和高阻故障,再利用第2级网络中的低阻故障测距网络和高阻故障测距网络分别对低阻故障和高阻故障的双端电气量进行数据融合,计算出精确的故障位置。对训练方法进行改进,在测量电气量中加入高斯白噪声信号来模拟含互感器误差的样本,将无误差样本和含误差样本组成的重复样本对作为训练样本,使训练后的BP神经网络对随机测量误差具备一定的适应能力。电磁暂态仿真结果表明:所提方法不受过渡电阻影响,在高阻故障情况下测距结果的最大误差仍然低于1%,且对随机误差具有较好的适应性,在输入电气量存在一定测量误差的情况下测距结果的最大误差低于2.5%,具有良好的应用前景。

关键词：输电线路故障测距 2级反向传播神经网络过渡电阻测量误差

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混凝土多轴等幅疲劳性能研究

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建筑材料学报 2020年第2期23卷 297-303页

作者：赵东拂赵润东常陆华宋玉普韩箫北京建筑大学土木与交通工程学院北京100044 军事科学院国防工程研究院北京100091 大连理工大学海岸与近海工程国家重点实验室辽宁大连116024 北京市市政专业设计院股份公司北京100037

利用大型多轴疲劳试验机,进行了双轴拉-压、三轴拉-压-压等幅循环荷载作用下混凝土的疲劳性能研究.分析了不同侧压比、不同应力水平下混凝土的多轴等幅疲劳破坏形态、疲劳强度以及残余应变的变化规律,得到了应力水平-疲劳寿命(S-N)曲线... 详细信息

利用大型多轴疲劳试验机,进行了双轴拉-压、三轴拉-压-压等幅循环荷载作用下混凝土的疲劳性能研究.分析了不同侧压比、不同应力水平下混凝土的多轴等幅疲劳破坏形态、疲劳强度以及残余应变的变化规律,得到了应力水平-疲劳寿命(S-N)曲线及其表达式.结果表明:在等幅疲劳荷载下,混凝土多轴疲劳破坏形态与侧压比无关;残余应变呈三阶段发展规律,受侧压比影响较大,几乎与应力水平无关.定义相对残余应变为损伤变量,建立了损伤演变方程,为混凝土多轴等幅疲劳试验研究及疲劳损伤评价提供参考.

关键词：混凝土双轴拉-压疲劳三轴拉-压-压疲劳疲劳强度疲劳损伤等幅

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