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西北植物学报 2007年第9期27卷 739-739页

作者： Photographed and introduced by Cao rui

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Aggregation-induced emission （ALE） dye loaded polymer nanoparticles for gene silencing in pancreatic cancer and their in vitro and in vivo biocompatibility evaluation

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Nano Research 2015年第5期8卷 1563-1576页

作者： rui Hu Chengbin Yang Yucheng Wang Guimiao Lin Wei Qin Qingling Ouyang Wing-Cheung Law Quoc toan Nguyen Ho Sup Yoon Xiaomei Wang Ken-tye Yong Ben Zhong tang School of Electrical and Electronic Engineering Nanyang Technological University Singapore 639798 Singapore the Engineering Lab of Synthetic Biology and the Key Lab of Biomedical Engineering School of Medicine Shenzhen University Shenzhen 518060 China Department of Chemistry The Hang Kong University of Science and Technology Clear Water Bay Kowloon Hang Kong China Department of Industrial and Systems Engineering The Hang Kong Polytechnic University Hung Ham Kowloon Hang Kong China Division of Structural Biology & Biochemistry School of Biological Sciences Nanyang Technological University Singapore 539798 Singapore

We have developed aggregation-induced emission （AIE） dye loaded polymer nanoparticles with deep-red emission for siRNA delivery to pancreatic cancer cells. two US food and Drug Administration （fDA） approved surfactant polymers, Pluronics f127 and PEGylated phospholipid, were used to prepare the dye-loaded nanoparticle formulations and they can be used as nanovectors for gene silencing of mutant K-ras in pancreatic cancer cells. the successful transfection of siRNA by the developed nanovectors was confirmed by the fluorescent imaging and quantified through flow cytometry. Quantitative real time polymerase chain reaction （PCR） indicates that the expression of the mutant K-ras oncogene from the MiaPaCa-2 pancreatic cancer cells has been successfully suppressed. More importantly, our in vivo toxicity study has revealed that both the nanoparticle formulations are highly biocompatible in BALC/c mice. Overall, our results suggest that the AIE dye-loaded polymer nanoparticle formulations developed here are suitable for gene delivery and have high potential applications in translational medicine research.

关键词： aggregation-induced emission polymer nanoparticles gene silencing pancreatic cancer

A modified laser-induced choroidal neovascularization animal model with intravitreal oxidized low-density lipoprotein

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International Journal of Ophthalmology(English edition) 2020年第8期13卷 1187-1194页

作者： tong Wu Kuan-Rong Dang Ya-fen Wang Bao-Zhen Lyu Wen-Qin Xu Guo-rui Dou Jian Zhou Yan-Nian Hui Hong-Jun Du Department of Ophthalmology Xijing HospitalEye Institute of Chinese PLAFourth Military Medical UniversityXi’an 710032Shaanxi ProvinceChina Department of Anatomy and Histology and Embryology Xi’an Health SchoolXi’an 710032Shaanxi ProvinceChina the Orbital Disease Institute of the third Medical Center of PLA General Hospital Beijing100039China

AIM: to investigate whether intravitreal injection of oxidized low-density lipoprotein(OxLDL) can promote laserinduced choroidal neovascularization(CNV) formation in mice and the mechanism involved, thereby to develop a better animal ***: C57BL6/J mice were randomized into three groups. Immediately after CNV induction with 532 nm laser photocoagulation, 1.0 μL of OxLDL [100 μg/m L in phosphate-buffered saline(PBS)] was intravitreally injected, whereas PBS and the same volume low-density lipoprotein(LDL;100 μg/m L in PBS) were injected into the vitreous as controls. Angiogenic and inflammatory cytokines were measured by quantitative real-time polymerase chain reaction(q Rt-PCR) and Western blotting(WB) after 5 d, and CNV severity was analyzed by choroid flat mount and immunofluorescence staining after 1wk. In vitro, retinal pigment epithelial(RPE) cell line(ARPE19) were treated with OxLDL(LDL as control) for 8 h. Angiogenic and inflammatory cytokine levels were measured. A specific inhibitor of lectin-like oxidized low-density lipoprotein receptor 1(LOX1) was used to evaluate the role of LOX1 in this ***: At 7 d after intravitreal injection of 1 μL(100 μg/mL) OxLDL, t15-labeled OxLDL was mainly deposited around the CNV area, and the f4/80-labeled macrophages, the CD31-labeled vascular endothelial cells number and CNV area were increased. Meanwhile, WB and qR t-PCR results showed that vascular endothelial growth factor(VEGf), CC chemokine receptor 2(CCR2), interleukin-6(IL-6), IL-1β, and matrix metalloproteinase 9(MMP9) expressions were increased, which was supported by in vitro experiments in RPE cells. LOX1 inhibitors significantly reduced expressions of inflammatory factors IL-1β and VEGf. CONCLUSION: A modified laser-induced CNV animal model is established with intravitreal injection of 1 μL(100 μg/mL) of OxLDL at 7 d, which at least partially through LOX1. this animal model can be used as a simple model for studying the role of OxLDL in age-

关键词： age-related macular degeneration choroidal neovascularization oxidized low-density lipoprotein animal model

一类非局部(p,q)-Laplace方程非负解的存在性

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郑州大学学报（理学版） 2016年第2期48卷 5-10页

作者：李瑞山西大学数学科学学院山西太原030006

研究了R^N中一类带有非局部项的(p,q)-Laplace方程非负解的存在性.在f(x,t)满足一定条件下,得到能量泛函Cerami序列的有界性,结合变分法证明了非负解的存在性.

关键词： (p,q)-Laplace方程非局部项 Cerami条件非负解

LHCGR and ALMS1 defects likely cooperate in the development of polycystic ovary syndrome indicated by double-mutant mice

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Journal of Genetics and Genomics 2021年第5期48卷 384-395页

作者： Li Yu Lina Wang Wufan tao Wenxiang Zhang Shuanghao Yang Jian Wang Jia fei rui Peng Yiming Wu Xiumei Zhen Hong Shao Weiyue Gu Rong Li Bai-Lin Wu Hongyan Wang Institutes of Biomedical Sciences Fudan UniversityShanghai 200032China Children’s Hospital Institutes of Reproduction and DevelopmentFudan UniversityShanghai 201102China Department of Laboratory Medicine Zhongshan HospitalFudan UniversityShanghai 200032China Center for Reproductive Medicine Department of Obstetrics and GynecologyPeking University Third HospitalBeijing 100191China Boston Children's Hospital and NGS collaboration Harvard Medical SchoolBoston MA 02115USA National Clinical Research Center for Obstetrics and Gynecology Peking University Third HospitalBeijing 100191China Obstetrics and Gynecology Hospital NHC Key Laboratory of Reproduction Regulation(Shanghai Institute of Planned Parenthood Research)State Key Laboratory of Genetic Engineering at School of Life SciencesInstitute of Metabolism and Integrative BiologyFudan UniversityShanghai 200011China Institute of Developmental Biology&Molecular Medicine Fudan UniversityShanghai 200433China Reproductive Medicine Centre Anhui Medical UniversityHefei 230032China Chigene translational Medicine Research Center Beijing 100875China Shanghai Children’s Medical Center Shanghai Jiaotong UniversityShanghai 200127China Zhongke Genetics and Reproductive Medicine Institute Beijing 102600China

Polycystic ovary syndrome(PCOS)is a heterogeneous disorder with evidence of polygenetic components,and obesity may be a risk factor for hyperandrogen *** studies have shown that LHCGR is en riched in the ovary and LHCGR deficiency causes infertility without typical PCOS ***1 is implicated in obesity and hyperandrogenism,the common phenotypes among PCOS *** whole-exome sequencing of 22 PCOS families and targeted candidate gene sequencing of additional 65 sporadic PCOS patients,we identified potential causative mutations in LHCGR and ALMS1 in a sibling-pair PCOS family and three sporadic PCOS *** expression of LHCGRL638 P in granulosa-like tumor cell line(KGN)cells promoted cyclic adenosine monophosphate production and granulosa cell proliferation,indicating that LHCGRL638 P is an activating ***~(L642 P/L642 P)mice showed an irregular estrous cycle,reduced follicles with dynamic folliculogenesis,and increased testosterone(t),estradiol(E2),and ***~(+/L642 P)AIms1~(+/PB)mice displayed increased t and E2 but decreased late secondary and preovulatory *** showed that activating mutation of LHCGR likely plays important roles in the pathophysiology of PCOS involving abnormal reproductive physiology,whereas ALMS1 deficiency may promote anovulatory infertility via elevated androgens,suggesting that the disturbed LHCGR and ALMS1 cooperatively induce PCOS phenotypes,characterized as anovulation and hyperandrogenemia frequently observed in PCOS patients with obesity.

关键词： LHCGR ALMS1 Anovulation Hyperandrogenemia PCOS

SARS-CoV-2 productively infects primary human immune system cells in vitro and in CoviD-19 patients

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Journal of Molecular Cell Biology 2022年第4期14卷 51-64页

作者： Marjorie C.Pontelli italo A.Castro Ronaldo B.Martins Leonardo La Serra flavio P.Veras Daniele C.Nascimento Camila M.Silva Ricardo S.Cardoso Roberta Rosales Rogério Gomes thais M.Lima Juliano P.Souza Brenda C.Vitti Diego B.Caetite Mikhael H.f.de Lima Spencer D.Stumpf Cassandra E.thompson Louis-Marie Bloyet Juliana E toller-Kawahisa Marcela C.Giannini Leticia P.Bonjorno Marial.f.Lopes Sabrina S.Batah Li Siyuan Rodrigo Luppino-Assad Sergio C.L.Almeida fabiola R.Oliveira Maira N.Benatti Lorena L.f.Pontes Rodrigo C.Santana fernando C.Vilar Maria Auxiliadora-Martins Pei-Yong Shi thiago M.Cunha Rodrigo t.Calado JoséC.Alves-filho Dario S.Zamboni Alexandre t.fabro Paulo Louzada-Junior Rene D.R.Oliveira Sean P.J.Whelan fernando Q.Cunha Eurico Arruda Virology Research Center Ribeirao Preto Medical SchoolUniversity of Sao Pauloibeirao PretoSao Paulo 14049-900.Brazil Center of Research in Inflammatory Diseases Ribeirao Preto Medical SchoolUniversity of Sao PauloRibeirao PretoSao Paulo 14049-900Brazil Department of Celland Molecular Biology Ribeirao Preto Medical SchoolUniversityof Sao PauloRibeirao PretoSao Paulo 14049-900 Brazil Regional Blood Centerof Ribeirao Preto Ribeirao Preto Medical ScholUniversity of Sao PauloRibeirao PretoSao Paulo 14049-900Brazil Department of Biochemistry&Molecular Biology the University of Texas Medical BranchGalvestonTX7755USA Divisions of Clinical lmmunology Infectious Diseases and Intensive Care UnitRibeirao Preto Medical Schooniversity of Sao PauloRibeirao PretoSao Paulo 14049-900Brazil Department of Pathology Ribeirao Preto Medical Scholniversity of Sao PauloRibeirao PretoSao Paulo 14049-900Brazil Department of Molecular Microbiology.Washington University in St.Louis Saint LouisMO 63110USA

the severe acute respiratory syndrome coronavirus 2(SARS-CovV-2)infection is associated with a hyperinflammatory state and lymphocytopenia,a hallmark that appears as both signature and prognosis of disease severity *** cytokine storm and a sustained inflammatory state are commonly associated with immune cell depletion,it is still unclear whether direct SARS-Cov-2 infection of immune cells could also play a role in this scenario by harboring viral *** found that monocytes,as well as both B and t lymphocytes,were susceptible to SARS-Cov-2 infection in vitro,accumulating double-stranded RNA consistent with viral RNA replication and ultimately leading to expressive t cell *** addition,flow cytometry and immunofluorescence analysis revealed that SARS-Cov-2 was frequently detected in monocytes and B lymphocytes from coronavirus disease 2019(CoVID-19)*** rates of SARS-Cov-2-infected monocytes in peripheral blood mononuclear cells from CoVID-19 patients increased over time from symptom onset,with SARS-CoV-2-positive monocytes,B cells,and CD4+t lymphocytes also detected in postmortem lung *** results indicated that SARS-CoV-2 infection of blood-circulating leukocytes in covID-19 patients might have important implications for disease pathogenesis and progression,immune dysfunction,and virus spread within the host.

关键词： SARS-CoV-2 CoVID-19 lymphocytopenia peripheral blood mononuclear cell(PBMC) lymphocytes monocytes apoptosis

Effect of Anisotropic Impurity Scattering in D-Wave Superconductors

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Chinese Physics Letters 2023年第5期40卷 83-87页

作者：王泽龙毛瑞盈王达王强华 National Laboratory of Solid State Microstructures&School of Physics Nanjing UniversityNanjing 210093China Collaborative Innovation Center of Advanced Microstructures Nanjing UniversityNanjing 210093China

In d_(x^(2)-y^(2))-wave superconductors,the effect of s-wave point disorder has been extensively studied in *** study the anisotropic disorder in the form of V_(kk')^(imp)=V_(if)_(kf)_(k')with f_(k)=cos(2θ)(θthe azimuthal angle of k),as proposed to be caused by apical oxygen vacancies in overdoped La-based cuprate films,under the Born *** disorder self-energy and d-wave pairing affect each other and must be solved simultaneously *** find that the self-energy is reduced at low frequencies and thus weakens the pair-breaking *** frequency dependence vanishes in the dirty limit for which the disorder is well described by a scattering rateΓ_(k)=Γ_(if)_(k)^(2).One consequence of the disorder effect is that the gap-to-t_c ratio 2Δ(0)/t_(c) is greatly enhanced by the d-wave disorder,much larger than the s-wave disorder and the clean BCS value ***,we generalize the d-wave scattering rate to a general formΓ_(θ)=Γ_α|θ-θ_(0)|^(α) around each nodal direction θ_(0).We find the density of statesρ(ω)-ρ(0)∝|ω|(ω^(2)) for allα≥1(αthe limit ofω→*** a result,the superfluid densityρ_(s) exhibits two and only two possible scaling behaviors:ρ_(s)(0)-ρ_(s)(t)∝t(t^(2))forα≥1(αthe low temperature limit.

关键词： effect limit breaking

太极拳运动对中老年人脑功能和有氧运动能力的影响

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中国康复理论与实践 2020年第6期26卷 637-642页

作者：姬瑞敏成都体育学院四川成都市610041

目的评价太极拳运动对中老年人有氧运动能力和脑认知功能的影响。方法2018年6月至2019年7月,将60例中老年人分为太极拳组(n=13)、冥想组(n=14)、有氧运动组(n=17)和久坐组(n=16),观察干预24周前后,各组体质量指数(BMI)、有氧运动能力以... 详细信息

目的评价太极拳运动对中老年人有氧运动能力和脑认知功能的影响。方法2018年6月至2019年7月,将60例中老年人分为太极拳组(n=13)、冥想组(n=14)、有氧运动组(n=17)和久坐组(n=16),观察干预24周前后,各组体质量指数(BMI)、有氧运动能力以及在执行认知任务过程中诱发的P300波形中P3b的振幅、潜伏期和P300反应时。结果干预后,各组BMI有非常高度显著性差异(f=7.568,Pt>3.039,Pt>2.152,Pt>2.667,Pf=0.419,P=0.740);太极拳组反应时短于其他三组,各组间无显著性差异(f=1.804,P>0.05)。结论太极拳运动可提高中老年人有氧运动能力,有利于改善认知功能。

关键词：太极拳有氧运动能力认知冥想

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锰锌铁氧体的低温合成及表征

材料工程 2016年第11期44卷 73-77页

作者：赵海涛王俏刘瑞萍马瑞廷沈阳理工大学材料科学与工程学院沈阳110159

采用溶胶-凝胶自燃烧法在低温下一步合成了纯相尖晶石结构的锰锌铁氧体(Mn0.5Zn0.5fe2O4)纳米颗粒。其结构、形貌和热分解过程分别采用X射线衍射仪(XRD)、扫描电镜(SEM)和tG-DSC分析仪进行了表征。结果表明:在pH=7.0、柠檬酸与金属离子... 详细信息

采用溶胶-凝胶自燃烧法在低温下一步合成了纯相尖晶石结构的锰锌铁氧体(Mn0.5Zn0.5fe2O4)纳米颗粒。其结构、形貌和热分解过程分别采用X射线衍射仪(XRD)、扫描电镜(SEM)和tG-DSC分析仪进行了表征。结果表明:在pH=7.0、柠檬酸与金属离子摩尔比为1∶1和柠檬酸的浓度为0.7mol/L的条件下,金属的硝酸盐和柠檬酸形成的干凝胶可通过自燃烧过程一步合成出平均粒径约为60nm的纯相Mn0.5Zn0.5fe2O4铁氧体纳米颗粒。经过400℃煅烧后,颗粒粒径增大,衍射峰变窄,强度增加,晶型更趋于完整。

关键词：锰锌铁氧体溶胶-凝胶自燃烧纳米颗粒

一类非线性三阶三点边值问题正解的存在性、不存在性及多解性

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山东大学学报（理学版） 2021年第12期56卷 52-58,66页

作者：张瑞燕西北师范大学数学与统计学院甘肃兰州730070

考虑一类非线性三阶三点边值问题{u^(m)(t)+λf(t,u(t))=0,t∈[0,1],u(0)=u′(0)=0,u′(1)=αu′(η)正解的存在性、不存在性以及多解性,其中λ>0是一个参数,0<η<1,1<α<1/η,f:[0,1]×[0,∞)→(0,∞)是一个连续函数。主要定理的证明... 详细信息

考虑一类非线性三阶三点边值问题{u^(m)(t)+λf(t,u(t))=0,t∈[0,1],u(0)=u′(0)=0,u′(1)=αu′(η)正解的存在性、不存在性以及多解性,其中λ>0是一个参数,0f:[0,1]×[0,∞)→(0,∞)是一个连续函数。主要定理的证明基于不动点指数理论、L(ray-Schauder度以及上下解方法。

关键词：三阶多解性存在性不动点指数