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Evaluation of pig oocyte in vitro maturation and fertilization using three gonadotropin-based hormonal compounds

Asian pacific Journal of Reproduction 2021年第2期10卷 90-97页

作者： rosario santiago-rodriguez Alma L.Alvarez-Guerrero Fernando Garcia-Gonzalez Alicia Alcantar-rodriguez Alfredo Medrano Autonomous University of Mexico State AmecamecaMexico Faculty of Sciences National Autonomous University of MexicoMexico National Autonomous University of Mexico FES Cuautitlan:Km 2.5 Carretera Cuautitlan-Teoloyucan54714 Cuautitlan IzcalliEstado de MexicoMexico

Objective:To evaluate the effect of human chorionic gonadotropins(hCG)and equine chorionic gonadotropins(eCG)on in vitro gilt oocyte maturation and embryonic development,using frozen semen for ***:Two independent experiments(6 replicates each)were carried out to evaluate gilt oocyte maturation,and fertilization and embryonic development by using ovaries from a local ***,712 oocytes were randomly distributed in four-well dishes to receive Novormon(eCG 5.0 IU),PG600(eCG 5.0 IU and hCG 2.5 IU),Chorulon(hCG 5.0 IU),or no *** were incubated with 5%CO2,95%air and saturation humidity at 39℃for 44 *** of the oocytes to metaphaseⅡwas assessed by using the aceto-orcein *** addition,741 oocytes were used and randomly distributed in four-well dishes,and then oocyte maturation was carried out as mentioned,but matured oocytes were washed and placed in fertilization medium with frozen-thawed *** were co-incubated for 7 h,and then washed and placed in development medium,and incubated for further 7 days,at which time embryonic development was *** and embryo development media were not supplemented with the studied ***:Novormon(eCG)and PG600(eCG+hCG)treatments significantly improved the percentages of metaphaseⅡoocytes compared to the control group(P<0.05).Furthermore,a significant increase was also observed in the young blastocyst stage between the control group and the PG600 treatment group(P<0.05).Conclusions:Hormonal products Novormon(eCG)and PG600(eCG+hCG)can obtain the highest percentages of in vitro maturation in gilt oocytes;however,this effect is not transferred to fertilization rates.

关键词： Chorionic gonadotropins Embryo Oocyte maturation In vitro fertilization

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Morphing wing flaps for large civil aircraft:Evolution of a smart technology across the Clean Sky program

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Chinese Journal of Aeronautics 2021年第7期34卷 13-28页

作者： rosario Pecora Department of Industrial Engineering Aerospace DivisionUniversity of Naples“Federico II”80125 NapoliItaly

Abstract Morphing wing structures are widely considered among the most promising technologies for the improvement of aerodynamic performances in large civil *** controlled adaptation of the wing shape to external operative conditions naturally enables the maximization of aircraft aerodynamic efficiency,with positive fallouts on the amount of fuel burned and pollutant *** benefits brought by morphing wings at aircraft level are accompanied by the criticalities of the enabling technologies,mainly involving weight penalties,overconsumption of electrical power,and safety *** attempt to solve such criticalities passes through the development of novel design approaches,ensuring the consolidation of reliable structural solutions that are adequately mature for certification and in-flight *** this work,the development phases of a multimodal camber morphing wing flap,tailored for large civil aircraft applications,are outlined with specific reference to the activities addressed by the author in the framework of the Clean Sky *** flap is morphed according to target shapes depending on aircraft flight conditions and defined to enhance high-lift performances during takeoff and landing,as well as wing aerodynamic efficiency during *** innovative system based on finger-like robotic ribs driven by electromechanical actuators is proposed as morphing-enabling technology;the maturation process of the device is then traced from the proof of concept to the consolidation of a true-scale demonstrator for pre-flight ground validation tests.A step-by-step approach involving the design and testing of intermediate demonstrators is then carried out to show the compliance of the adaptive system with industrial standards and safety *** technical issues encountered during the development of each intermediate demonstrator are critically analyzed,and justifications are provided for all the adopted engineering ***,the layout

关键词： Electro-mechanical actuators(EMA) Green regional aircraft Mechanical systems Morphing flap Morphing wing Smart aircraft Smart structures Variable camber airfoil

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Recent advances in bird sperm morphometric analysis and its role in male gamete characterization and reproduction technologies

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Asian Journal of Andrology 2016年第6期18卷 882-888页

作者： Julian santiago-Moreno Milagros Cristina Esteso Silvia Villaverde-Morcillo Adolfo Toledano-Diaz Cristina Castafio rosario Velazquez Antonio Lopez-Sebastian Agustin Lopez Goya Javier Gimeno Martinez Department of Animal Reproduction INIA 28040 Madrid Spain Veterinary Clinical and Research Services Fieb Foundation 28760 Tres Cantos Madrid Spain FAUNIA 28032 Madrid Spain

Postcopulatory sexual selection through sperm competition may be an important evolutionary force affecting many reproductive traits, including sperm morphometrics. Environmental factors such as pollutants, pesticides, and climate change may affect different sperm traits, and thus reproduction, in sensitive bird species. Many sperm-handling processes used in assisted reproductive techniques may also affect the size of sperm cells. The accurately measured dimensions of sperm cell structures （especially the head） can thus be used as indicators of environmental influences, in improving our understanding of reproductive and evolutionary strategies, and for optimizing assisted reproductive techniques （e.g., sperm cryopreservation） for use with birds. Computer-assisted sperm morphometry analysis （CASA-Morph） provides an accurate and reliable method for assessing sperm morphometry, reducing the problem of subjectivity associated with human visual assessment. Computerized systems have been standardized for use with semen from different mammalian species. Avian spermatozoa, however, are filiform, limiting their analysis with such systems, which were developed to examine the approximately spherical heads of mammalian sperm cells. To help overcome this, the standardization of staining techniques to be used in compute^-assessed light microscopical methods is a priority. The present review discusses these points and describes the sperm morphometric characteristics of several wild and domestic bird species.

关键词： avian computer-assisted assay morphometry spermatozoa

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Benefits Realization Management Framework to Ensure the Creation and Timely Delivery of Public Value in Uruguay

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Belt and Road Initiative Tax Journal 2022年第1期3卷 95-103页

作者： Maria del rosario rodriguez Barbieri Planning and Management Control Department Direccion General Impositiva Uruguay

The aim of this article is to share the experience in enhancing tax administration capacity building to manage benefits *** 2020 the Uruguayan Tax Administration implemented the Benefits Realization Management framework through the application of the DGI Business Case Preparation Methodology and the DGI Benefits Management General *** article describes the main characteristics of both methodologies,their implementation process,the results obtained,and the actions that have been planned to be carried out to consolidate such practices in the *** also analyzes the relationship between benefits realization,organizational strategy,portfolio management,and public value creation.

关键词： Benefit Realization Management Business Case Tax administration Public value

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The identification of wildlife-vehicle collision hotspots: Citizen science reveals spatial and temporal patterns

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Ecological Processes 2021年第1期10卷 99-111页

作者： Francesco Valerio Marco Basile rosario Balestrieri Mediterranean Institute for Agriculture Environment and DevelopmentInstituto de Investigação e Formação AvançadaUniversidade deÉvoraPólo da MitraAp.947006-554ÉvoraPortugal. UBC-Unidade de Biologia da Conservação Departamento de BiologiaUniversidade deÉvoraPólo da MitraAp.947002-554ÉvoraPortugal. Associazione per la Ricerca la Divulgazione e l’Educazione Ambientale–ARDEAVia Ventilabro80126 NapoliItaly. Chair of Wildlife Ecology and Management University of FreiburgTennenbacher Str.479106 FreiburgGermany. Stazione Zoologica Anton Dohrn Villa Comunale 180121 NaplesItaly.

Linear infrastructures(e.g.,roads,railways,pipelines,and powerlines)pose a serious threat to wildlife,due to the risk of wildlife-vehicle collisions(roadkills).The placement of mitigation measures,such as crossing structures,should consider species’life cycles and ecological *** an assessment would require data collection over large areas,which may be possible by employing citizen *** this study,we aimed to identify spatiotemporal trends of roadkill occurrence using citizen science data from one of the most urbanized and biodiversityrich regions of *** trends were analyzed using generalized additive models,while landscape patterns were assessed by identifying significant thresholds over land cover gradients,related to increases in relative roadkill abundance,by employing threshold indicator taxa *** approach recorded a total of 529 roadkills,including 33 different species,comprising 13 mammal,10 bird,6 reptile,and 2 amphibian *** analysis indicated significant temporal trends for the red fox,the European hedgehog,the stone marten and the European badger,with peaks in roadkill occurrence between the winter and spring *** roadkill abundance increased mostly in landscapes with anthropogenic land cover classes,such as complex cultivations,orchards,or urban *** results allowed us to develop a map of potential roadkill risk that could assist in planning the placement of mitigation *** science contributions from highly populated areas allowed data collection over a large area and a dense road network,and also directly led to the evaluation of management decisional options.

关键词： Roadkill Land cover Road ecology Seasonal trend Spatial prioritization Urban wildlife

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Potential impact of genetically modified Lepidoptera-resistant Brassica napus in biodiversity hotspots： Sicily as a theoretical model

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Insect Science 2018年第4期25卷 562-580页

作者： Barbara Manachini Giuseppe Bazan rosario Schicchi Department of Agricultural Food and Forest Sciences University of Palermo Palermo Italy Department of Biological Chemical and Pharmaceutical Sciences and Technologies University of Palermo Palermo Italy

The general increase of the cultivation and trade of Bt transgenic plants resis- tant to Lepidoptera pests raises concerns regarding the conservation of animal and plant biodiversity. Demand for biofuels has increased the cultivation and importation of oilseed rape （Brassica napus L.）, including transgenic lines. In environmental risk assessments （ERAs） for its potential future cultivation as well as for food and feed uses, the impact on wild Brassicaeae relatives and on non-target Lepidoptera should be assessed. Here we consider the potential exposure of butterflies as results of possible cultivation or natural- ization of spilled seed in Sicily （Italy）. Diurnal Lepidoptera, which are pollinators, can be exposed directly to the insecticidal proteins as larvae （mainly of Pieridae） through the host and through the pollen that can deposit on other host plants. Adults can be exposed via pollen and nectar. The flight periods of butterflies were recorded, and they were found to overlap for about 90% of the flowering period ofB. napus for the majority of the species. In addition, B. napus has a high potential to hybridise with endemic taxa belonging to the B. oleracea group. This could lead to an exposure of non-target Lepidoptera if introgression of the Bt gene into a wild population happens. A rank of the risk for butterflies and wild relatives of oilseed rape is given. We conclude that, in environmental risk assessments, attention should be paid to plant-insect interaction especially in a biodiversity hotspot such as Sicily.

关键词： butterflies cross pollination non-target effect oilseed rape protected areas transgenic crop

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Use of two-surfactants mixtures to attain specific HLB values for assisted TPH-diesel biodegradation

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Journal of Environmental Sciences 2004年第6期16卷 950-956页

作者： Luis G. Torres Neftalí Rojas rosario Iturbe Instituto de Ingeniería Universidad Nacional Autó noma de México Coordinación de Ingeniería Ambiental Grupo Saneamiento de Suelos y Acuíferos Apartado Postal 70-472 Coyoacán 04510 México D.F. Mexico

In a surfactant assisted biodegradation process, the choice of surfactant(s) is of crucial importance. The question is: does the type of surfactant(i.e. chemical family) affect the biodegradation process at fixed hidrophillic-lypofillic balance(HLB) values? Microcosm assessments were developed using contaminated soil, with around of 5000 mg/kg of hydrocarbons as TPH-diesel. Mixtures of three nonionic surfactants were employed to get a wide range of specific HLB values. Tween20 and Span20 were mixed in the appropriate proportions to get HLB values between 8.6 and 16.7. Tween/Span60 mixtures reached HLB values between 4.7 and 14.9. Finally, Tween/Span80 combinations yielded HLB values between 4.3 and 15. TPH-diesel biodegradation was measured at the beginning, and after 8 weeks, as well as the FCU/gr soil, as a measure of microorganisms′ development during the biodegradation period. A second aim of this work was to assess the use of guar gum as a biodegradation enhancer instead of synthetic products. The conclusions of this work are that surfactant chemical family, and not only the HLB value clearly affects the assisted biodegradation rate. Surfactant's synergism was clearly observed. Regarding the use of guar gum, no biodegradation enhancement was observed for the three assessed concentrations, i.e., 2, 20, and 200 mg/kg, respectively. On the contrary, TPH-diesel removal was lower as the gum concentration increased. It is quite possible that guar gum was used as a microbial substrate.

关键词： aged soils enhanced bioremediation HLB mixtures Tween Span surfactants

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Lyapunov stability of competitive cells dynamics in tumor mechanobiology

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Acta Mechanica Sinica 2021年第2期37卷 244-263页

作者： Angelo rosario Carotenuto Arsenio Cutolo Stefania Palumbo Massimiliano Fraldi Department of Structures for Engineering and Architecture University of Napoli Federico IINapoliItaly

Poromechanics plays a key role in modelling hard and soft tissue behaviours,by providing a thermodynamic framework in which chemo-mechanical mutual interactions among fluid and solid constituents can be consistently rooted,at different scale *** this context,how different biological species(including cells,extra-cellular components and chemical metabolites)interplay within complex environments is studied for characterizing the mechanobiology of tumor growth,governed by intra-tumoral residual stresses that initiate mechanotransductive processes deregulating normal tissue homeostasis and leading to tissue *** the coupling between tumor poroelasticity and interspecific competitive dynamics has recently highlighted how microscopic cells and environment interactions influence growth-associated stresses and tumor pathophysiology,the nonlinear interlacing among biochemical factors and mechanics somehow hindered the possibility of gaining qualitative insights into cells *** by this,in the present work we recover the linear poroelasticity in order to benefit of a reduced complexity,so first deriving the well-known Lyapunov stability criterion from the thermodynamic dissipation principle and then analysing the stability of the mechanical competition among cells fighting for common space and resources during cancer growth and *** the end,the linear poroelastic model enriched by interspecific dynamics is also exploited to show how growth anisotropy can alter the stress field in spherical tumor masses,by thus indirectly affecting cell mechano-sensing.

关键词： Tumor growth Poroelasticity Cells competition Volterra-Lotka dynamics Stability

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Inflammatory bowel disease in pediatric and adolescent patients:A biomolecular and histopathological review

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World Journal of Gastroenterology 2014年第30期20卷 10262-10278页

作者： Luciana Rigoli rosario Alberto Caruso Department of Pediatrics University of MessinaI-98125 MessinaItaly Department of Human Pathology University of MessinaI-98125 MessinaItaly

Crohn’s disease (CD) and ulcerative colitis (UC) are the two main forms of inflammatory bowel disease (IBD) with both overlapping and distinct clinical, pathological and biomolecular features. It has been suggested that pediatric IBD is a distinct disease entity, with probably different disease *** aim of this study is to review and summarize the evolution of the current concept of pediatric IBD. The results of this review reinforce the idea that pediatric CD and UC may be further classified in various clinicopathologic entities. For clinicians and pathologists convenience, practical algorithms for the distinction of the various subphenotypes of pediatric IBD are also provided.

关键词： Pediatric inflammatory bowel disease Crohn's disease Ulcerative disease Histopathology Molecular biology

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Mitochondrial DNA alterations in the progression of gastric carcinomas:Unexplored issues and future research needs

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World Journal of Gastroenterology 2014年第43期20卷 16159-16166页

作者： Luciana Rigoli rosario Alberto Caruso Department of Pediatrics School of MedicineUniversity of Messina98122 MessinaItaly Department of Human Pathology School of MedicineUniversity of Messina98122 MessinaItaly

Gastric cancer is the second most frequent cause of cancer death *** infected with Helicobacter pylori(***)are at increased risk of gastric *** induces genomic instability in both nuclear and mitochondrial(mt)DNA of gastric epithelial *** in mt DNA represent an early event during gastric tumorigenesis,and thus may serve as potential biomarkers for early detection and prognosis in gastric *** review article summarizes the mt DNA mutations that have been reported in gastric carcinomas and their precancerous *** research topics,such as the role of mt DNA alterations in an alternative pathway of gastric carcinogenesis,are identified and directions for future research are suggested.

关键词： Gastric carcinoma Precancerous lesions Histopathology mtDNA Molecular biology

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