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Culture-free identification of fast-growing cyanobacteria cells by Raman-activated gravity-driven encapsulation and sequencing

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Synthetic and Systems Biotechnology 2023年第4期8卷 708-715页

作者： Jinyu Cui rongze chen Huili Sun Yingyi Xue Zhidian Diao Jingyun Song Xiaohang Wang Jia Zhang chen Wang Bo Ma Jian Xu Guodong Luan Xuefeng Lu Key Laboratory of Biofuels Qingdao Institute of Bioenergy and Bioprocess TechnologyChinese Academy of SciencesNo.189 Songling RoadQingdao266101China Shandong Energy Institute Qingdao266101China Qingdao New Energy Shandong Laboratory Qingdao266101China College of Life Science University of Chinese Academy of SciencesBeijingChina Dalian National Laboratory for Clean Energy Dalian116023China Laboratory for Marine Biology and Biotechnology Qingdao National Laboratory for Marine Science and TechnologyQingdaoChina

By directly converting solar energy and carbon dioxide into biobased products,cyanobacteria are promising chassis for photosynthetic *** make cyanobacterial photosynthetic biosynthesis technology economically feasible on industrial scales,exploring and engineering cyanobacterial chassis and cell factories with fast growth rates and carbon fixation activities facing environmental stresses are of great *** simplify and accelerate the screening for fast-growing cyanobacteria strains,a method called Individual Cyanobacteria Vitality Tests and Screening(iCyanVS)was *** show that the ^(13)C incorporation ratio of carotenoids can be used to measure differences in cell growth and carbon fixation rates in individual cyanobacterial cells of distinct genotypes that differ in growth rates in bulk cultivations,thus greatly accelerating the process screening for fastest-growing *** feasibility of this approach is further demonstrated by phenotypically and then genotypically identifying individual cyanobacterial cells with higher salt tolerance from an artificial mutant library via Raman-activated gravity-driven encapsulation and ***,this method should find broad applications in growth rate or carbon intake rate based screening of cyanobacteria and other photosynthetic cell factories.

关键词： Cyanobacteria Single-cell Raman microspectroscopy Stable isotope probing Carotenoids Identification

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山西沁县方言古来母[i]韵字读卷舌元音的现象

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中国语文 2022年第1期 72-78,127页

作者：陈荣泽西藏民族大学文学院咸阳

山西沁县方言古来母[i]韵字读卷舌元音的现象颇具特色。古来母[i]韵字来自部分遇摄合口三等来母字和止摄开口三等来母字。部分遇合三来母字的白读音为[li],与止开三来母字合流,之后[i]在舌尖化之前就先行脱落,从而导致[l]成为自成音节的... 详细信息

山西沁县方言古来母[i]韵字读卷舌元音的现象颇具特色。古来母[i]韵字来自部分遇摄合口三等来母字和止摄开口三等来母字。部分遇合三来母字的白读音为[li],与止开三来母字合流,之后[i]在舌尖化之前就先行脱落,从而导致[l]成为自成音节的[■],后经过卷舌化的[■]阶段,最终演变为[■r],即[li]>[■]>[■>[■r]。在汉语北方方言止开三日母字读[■]/[■]的四种类型中,郑州、平顺、淄川三种类型的音变过程为[z]>[l]>[■]>[■],武乡型则为[z]>[l]>[■]。沁县方言中的卷舌元音是武乡型方言继续演变的结果,其音变过程是部分汉语北方方言形成卷舌元音的一条主要途径,在汉语方言[■r]音发展史中具有重要的类型学意义。

关键词：沁县方言古来母 [i]韵卷舌元音类型学意义

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Artificial intelligence-assisted automatic and index-based microbial single-cell sorting system for One-Cell-One-Tube

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mLife 2022年第4期1卷 448-459页

作者： Zhidian Diao Lingyan Kan Yilong Zhao Jingyun Song chen Wang Yang Liu Fengli Zhang Teng Xu rongze chen Xixian Wang Xiaoyan Jing Jian Xu Yuandong Li Bo Ma CAS Key Laboratory of Biofuels Shandong Key Laboratory of Energy GeneticsSingle-Cell CenterQingdao Institute of Bioenergy and Bioprocess TechnologyChinese Academy of SciencesQingdaoChina University of Chinese Academy of Sciences BeijingChina Shandong Energy Institute QingdaoChina Qingdao New Energy Shandong Laboratory QingdaoChina.5Qingdao Single-Cell Biotechnology Co.Ltd.QingdaoChina.

Identification,sorting,and sequencing of individual cells directly from in situ samples have great potential for in-depth analysis of the structure and function of *** this work,based on an artificial intelligence(AI)-assisted object detection model for cell phenotype screening and a cross-interface contact method for single-cell exporting,we developed an automatic and index-based system called EasySort AUTO,where individual microbial cells are sorted and then packaged in a microdroplet and automatically exported in a precisely indexed,“One-Cell-One-Tube”*** target cell is automatically identified based on an AI-assisted object detection model and then mobilized via an optical tweezer for ***,a crossinterface contact microfluidic printing method that we developed enables the automated transfer of cells from the chip to the tube,which leads to coupling with subsequent single-cell culture or *** efficiency of the system for single-cell printing is>93%.The throughput of the system for single-cell printing is~120 cells/***,>80%of single cells of both yeast and Escherichia coli are culturable,suggesting the superior preservation of cell viability during ***,AI-assisted object detection supports automated sorting of target cells with high accuracy from mixed yeast samples,which was validated by downstream single-cell proliferation *** automation,index maintenance,and vitality preservation of EasySort AUTO suggest its excellent application potential for single-cell sorting.

关键词： artificial intelligence One-Cell-One-Tube(OCOT) single-cell analysis single-cell printing single-cell sorting

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藏语地理分布格局的形成原因

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西藏研究 2016年第5期 108-113页

作者：陈荣泽西藏民族大学文学院陕西咸阳712082

藏语是藏族最主要的交际工具,其地理分布辽阔。藏语地理分布格局的形成主要与人口迁徙、民族融合和自然环境密切联系。吐蕃时期藏族向周边地区的大规模人口迁徙奠定了藏语地理分布格局的基础。在吐蕃政权及其以后的历史时期中,藏族与周... 详细信息

藏语是藏族最主要的交际工具,其地理分布辽阔。藏语地理分布格局的形成主要与人口迁徙、民族融合和自然环境密切联系。吐蕃时期藏族向周边地区的大规模人口迁徙奠定了藏语地理分布格局的基础。在吐蕃政权及其以后的历史时期中,藏族与周围的民族发生了民族融合,由民族融合引起的语言融合使藏语的地理分布格局日趋定型。青藏高原的自然环境从根本上决定了藏族文明的发展,从而也决定了藏语的地理分布格局。藏语的地理分布格局是多种因素在漫长的历史长河中相互作用下而逐渐形成的。

关键词：藏语地理分布人口迁徙民族融合自然环境

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Multiple routes toward engineering efficient cyanobacterial photosynthetic biomanufacturing technologies

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Green Carbon 2023年第2期1卷 210-226页

作者： Jinyu Cui Huili Sun rongze chen Jiahui Sun Guanlan Mo Guodong Luan Xuefeng Lu Key Laboratory of Biofuels Qingdao Institute of Bioenergy and Bioprocess TechnologyChinese Academy of SciencesNo.189 Songling RoadQingdao 266101China Shandong Energy Institute Qingdao 266101China Qingdao New Energy Shandong Laboratory Qingdao 266101China College of Life Science University of Chinese Academy of SciencesBeijing 100049China Dalian National Laboratory for Clean Energy Dalian 116023China Laboratory for Marine Biology and Biotechnology Qingdao National Laboratory for Marine Science and TechnologyQingdao 266237China

Developing efficient CO_(2)utilization technologies can alleviate the urgent pressure on energy and the ***,these technologies are crucial for achieving the goal of net zero *** are photoautotrophic microorganisms that are the main sources of primary productivity in the ***,the only prokaryotic microalgae,have also been considered as promising chassis for photosynthetic biosynthesis,directly converting solar energy and CO_(2)into various bio-based *** technological route is called photosynthetic biomanufacturing,and is advantageous to simultaneous carbon fixation and clean *** review focuses on development mode,application and suggests trends related to the further development of photosynthetic *** regard to the link between photosynthetic CO_(2)fixation and the production of desired metabolites,we summarized and compared three widely adopted strategies.“Screening to find”,screening a large number of high-quality cyanobacterial resources and analyzing their intracellular metabolites are of significance for screening novel cyanobacterial species with high-value chemicals and properties of industrial relevance.“Engineering to modify”,the emergence and application of synthetic biological tools and metabolic engineering strategies have enhanced the ability to modify different cyanobacterial species to reshape more carbon to flow toward synthetic tailored chemicals.“Stressing to activate”,through special culture conditions and strategies,combined with omics analysis techniques,silent metabolic pathways and functional modules are activated to induce the accumulation of high-value *** review provides valid and updated information to facilitate the development of photosynthetic biosynthesis route with carbon fixation and clean production,providing specific feasible solutions for net zero emissions.

关键词： Cyanobacteria Photosynthetic biomanufacturing Net zero emissions Metabolic engineering Stressing

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西安阎良谭家堡山东话语音的内部差异

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咸阳师范学院学报 2016年第1期31卷 48-53页

作者：陈荣泽西藏民族大学文学院陕西咸阳712082

西安阎良谭家堡是关中现存最大的山东方言岛,其居民主要来自今山东高密、昌邑一带。本文重点描写谭家堡山东话语音的内部差异,其内部差异主要表现在年龄上。其中,老中派保留源方言的特点较多,受陕西话的影响较小,中派保留源方言的特点... 详细信息

西安阎良谭家堡是关中现存最大的山东方言岛,其居民主要来自今山东高密、昌邑一带。本文重点描写谭家堡山东话语音的内部差异,其内部差异主要表现在年龄上。其中,老中派保留源方言的特点较多,受陕西话的影响较小,中派保留源方言的特点在逐渐减少,受陕西话的影响较大,青派则是源方言的好些语音特点已经消失,其语音有较为明显的普通话化倾向。

关键词：谭家堡山东话语音内部差异年龄差异

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Rapid, automated, and reliable antimicrobial susceptibility test from positive blood culture by CAST‐R

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mLife 2022年第3期1卷 329-340页

作者： Pengfei Zhu Lihui Ren Ying Zhu Jing Dai Huijie Liu Yuli Mao Yuandong Li Yuehui He Xiaoshan Zheng rongze chen Xiaoting Fu Lili Zhang Lijun Sun Yuanqi Zhu Yuetong Ji Bo Ma Yingchun Xu Jian Xu Qiwen Yang Single‐Cell Center CAS Key Laboratory of BiofuelsShandong Key Laboratory of Energy Genetics and Shandong Energy InstituteQingdao Institute of Bioenergy and Bioprocess TechnologyChinese Academy of SciencesQingdaoChina University of Chinese Academy of Sciences BeijingChina College of Information Science&Engineering Ocean University of ChinaQingdaoChina Department of Clinical Laboratory Peking Union Medical College HospitalPeking Union Medical CollegeChinese Academy of Medical SciencesBeijingChina Graduate School Peking Union Medical CollegeChinese Academy of Medical SciencesBeijingChina Department of Clinical Laboratory Affiliated Hospital of Qingdao UniversityQingdao UniversityQingdaoChina Qingdao Single‐Cell Biotechnology Co.Ltd.QingdaoChina The Bioland Laboratory GuangzhouChina

Antimicrobial susceptibility tests(ASTs)are pivotal in combating multidrug resistant pathogens,yet they can be time‐consuming,labor‐intensive,and *** the AST of tigecycline for sepsis as the main model,here we establish an automated system of Clinical Antimicrobials Susceptibility Test Ramanometry(CAST‐R),based on D2O‐probed Raman mi*** a liquid robot for sample pretreatment and a machine learning‐based control scheme for data acquisition and quality control,the 3‐h,automated CAST‐R process accelerates AST by>10‐fold,processes 96 paralleled antibiotic‐exposure reactions,and produces high‐quality Raman *** Expedited Minimal Inhibitory Concentration via Metabolic Activity is proposed as a quantitative and broadly applicable parameter for metabolism‐based AST,which shows 99%essential agreement and 93%categorical agreement with the broth microdilution method(BMD)when tested on 100 Acinetobacter baumannii *** tests on 26 clinically positive blood samples for eight antimicrobials,including tigecycline,meropenem,ceftazidime,ampicillin/sulbactam,oxacillin,clindamycin,vancomycin,and levofloxacin reveal 93%categorical agreement with BMD‐based *** automation,speed,reliability,and general applicability of CAST‐R suggest its potential utility for guiding the clinical administration of antimicrobials.

关键词： antimicrobial susceptibility test laboratory automation Raman sepsis tigecycline

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