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Involvement of NAC transcription factor NaNAC29 in Alternaria alternata resistance and leaf senescence in Nicotiana attenuata

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Plant Diversity 2021年第6期43卷 502-509页

作者： Lan Ma rongping li Luoyan Ma Na Song Zhen Xu Jinsong Wu Yunnan Key Laboratory for Wild Plant Resources Kunming Institute of BotanyChinese Academy of SciencesLanhei Road 132650201KunmingChina University of Chinese Academy of Science Beijing 100049China

NAC-liKE,ACTIVATED BY AP3/PI(NAP)is a NAC transcription factor regulating leaf senescence in Arabidopsis *** wild tobacco Nicotiana attenuata,a nuclear localized NAC transcription factor NaNAC29 was identified to be highly elicited after inoculation of Alternaria alternata,a notorious necrotic fungus on tobacco *** NaNAC29 possesses similar tertiary structure to NAP with 60%amino acid ***,it remains unknown the role of NaNAC29 in plant defense responses to *** and leaf senescence in *** this paper,Defensin-like protein 1(NaDLP1)was highly induced in *** after *** inoculation and bigger lesions were developed in NaDLP1-silenced ***,***-induced NaDLP1 was reduced by 76%in VIGS NaNAC29 plants and by 61%in JA deficient irAOC plants at 3 days post *** regulation of NaDLP1 expression by NaNAC29 was clearly independent on JA pathway,since exogenous methyl jasmonate treatment could not complement the induction levels of NaDLP1 in NaNAC29-silenced plants to the levels in WT ***,the expression of NaNAC29 was low expressed in young leaves but highly in senescent leaves and darktreated ***29-silenced plants,which were generated by virus-induced gene silencing(VIGS NaNAC29),showed delayed senescence *** addition,constitutive over-expression of NaNAC29 in *** could rescue the delayed-senescence phenotype of nap and caused precocious leaf senescence of wild-type Col-0 *** the data above demonstrate that NaNAC29 is a NAP homolog in *** participating in the defense responses to *** by regulation of a defensin protein NaDLP1 and promoting leaf senescence.

关键词： NaNAC29 Defensin-like protein 1 Tobacco Brown spot disease Aging

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Expression of IL-1α, IL-6, TGF-β, FasL and ZNF265 During Sertoli Cell Infection by Ureaplasma Urealyticum

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Cellular & Molecular Immunology 2009年第3期6卷 215-221页

作者： rongping li Yebin Xi Xiuzhi liu Guangjie Chen Baoguo Wang lihua Jiang Weiyi li Shanghai Jiao Tong University School of Medicine Department of ImmunoLogy Shanghai Institute of Immunology Shanghai 200025 China

To investigate immunoregulatory mechanisms of Sertoli cells in the testis in vitro and in vivo, we utilized our well-characterized Ureaplasma Urealyticum （UU）-induced model. We investigated the expressions of IL-1α, IL-6, TGF-β, FasL and ZNF265 at the first, second and third weeks post-infection. During recovery from inflammation and with the help of negative regulators TGF-β and FasL, the high levels of IL-1α and IL-6 expressions were observed in the early stages of the infection, and decreased gradually in the later weeks both in vitro and in vivo. The trend of varied expression of ZNF265 was similar to those of TGF-β and FasL in vitro and in vivo for Sertoli cells infected with UU.

关键词： ZNF265 TGF-β FasL Sertoli cell immunoregulation

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Effects of Optimized Root Water Uptake Parameterization Schemes on Water and Heat Flux Simulation in a Maize Agroecosystem

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Journal of Meteorological Research 2017年第2期31卷 363-377页

作者： Fu CAI Huiqing MING Na MI Yanbing XIE Yushu ZHANG rongping li Institute of Atmospheric Environment China Meteorological Administration Shenyang 110166 liaoning Province Meteorological Service Center Shenyang 110166 Institute of Meteorological Science of liaoning Province Shenyang 110166

As root water uptake（RWU）is an important link in the water and heat exchange between plants and ambient air,improving its parameterization is key to enhancing the performance of land surface model *** different types of RWU functions have been adopted in land surface models,there is no evidence as to which scheme most applicable to maize farmland *** on the 2007–09 data collected at the farmland ecosystem field station in Jinzhou,the RWU function in the Common Land Model（Co LM）was optimized with scheme options in light of factors determining whether roots absorb water from a certain soil layer（W_x）and whether the baseline cumulative root efficiency required for maximum plant transpiration（W_c）is *** sensibility of the parameters of the optimization scheme was investigated,and then the effects of the optimized RWU function on water and heat flux simulation were *** results indicate that the model simulation was not sensitive to W_x but was significantly impacted by W_*** the original model,soil humidity was somewhat underestimated for precipitation-free days;soil temperature was simulated with obvious interannual and seasonal differences and remarkable underestimations for the maize late-growth stage;and sensible and latent heat fluxes were overestimated and underestimated,respectively,for years with relatively less precipitation,and both were simulated with high accuracy for years with relatively more *** optimized RWU process resulted in a significant improvement of Co LM’s performance in simulating soil humidity,temperature,sensible heat,and latent heat,for dry *** conclusion,the optimized RWU scheme available for the Co LM model is applicable to the simulation of water and heat flux for maize farmland ecosystems in arid areas.

关键词： root water uptake land surface model water flux heat flux maize agroecosystem

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Carbon Budget Dynamics over a Rain-Fed Maize Agricultural Ecosystem in Northeast China and Its Regulation

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Open Journal of Ecology 2017年第6期7卷 377-391页

作者： rongping li Guangsheng Zhou li Zhou Yang Yang liaoning Institute of Meteorological Science Shenyang China Chinese Academy of Meteorological Sciences Beijing China Jinzhou Ecological and Agricultural Meteorology Center Jinzhou China

Based on the eddy-covariance observation data over rain-fed maize agricultural ecosystem during 2005-2011, the dynamics of net ecosystem CO2 exchange (NEE) and its control mechanism were analyzed in the present study. We found that the average carbon budget of non-growing season, growing season and annual were 153.16 - 202.03 g C/m2, −689.36 - −488.17 g C/m2, and −316.96 - −487.33 g C/m2, respectively. Maize carbon content of grain yield was −226.6 - −339.94 g C/m2, accounting for 55.4% of carbon budget in the growing season. From sowing to seven-leaf stage, the carbon budget of this ecosystem was characterized by carbon release, with the rate of 0.028 ±0.0056 mg CO2 m−2⋅s−1. From seven-leaf to mature stage, the carbon budget was characterized by carbon absorption, with the rate of −0.256 ±0.0693 mg CO2 m−2⋅s−1. The key meteorological factors affecting annual carbon budget included daily average temperature (R = −0.81, P = 0.03) and saturated vapor pressure deficit (R = −0.64, P = 0.12). At the same photosynthetically active radiation (PAR) level, CO2 assimilation rate was linearly correlated with leaf area index (P likely to be resulted from the extension of growing season. On the contrary, the carbon sink of rain-fed maize ecosystem in growing season might be decreased by extending the growing season ahead of the sowing date.

关键词： Rain-Fed Maize Carbon Budget Dynamics Regulation Mechanism Northeast China

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Investigation of ＂Four-Two-Three＂ System of Individualization Fostering Model -Taking College of Economics and Management of Hebei University of Science and Technology as an Example

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International English Education Research 2014年第3期 79-83页

作者： rongping li Yan Gao liqun Zhang College of Economics and Management Hebei University of Science and Technology Shijiazhuang Hebei China 050018

＂Four-Two-Three＂ training mode system, based on the social development needs of talent personalized, divides the training process of eight semesters in four years into ＂category training＂, ＂professional training＂ and ＂personalized training＂ and phased build talent training mode of ＂four disciplines platform ＋ two capability group lessons ＋ three development path＂. In this paper, we take College of Economics and Management, Hebei University of Science and Technology as an example, explained principles, fi＇amework and content, and measures of personnel training mode and other issues in details and made a smooth implementation of security mechanism in training mode.

关键词： "Four-Two-Three" System Personalized Training Mode

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Control Mechanisms and Simulation of <i>Populus simonii</i>Leaf Unfolding

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Journal of Geoscience and Environment Protection 2017年第8期5卷 41-55页

作者： rongping li Ting Wang Shoujun Sun Dongming liu Qi Zhang liaoning Institute of Meteorological Science Shenyang China Heilongjiang Institute of Meteorological Science Haerbin China

Populus simonii Carr., one of the main poplar tree species, is cultivated widely in Northeast and Northwest China in protection and timber forests. Plant phenology plays an important role in timber production by controlling the growing period (i.e., the period between the leaf unfolding and the leaf turning yellow). It is important to understand this control mechanism and to improve the accuracy of the simulation of leaf unfolding phenology for P. simonii in order to determine accurately the timber production of P. simonii plantations. In this study, based on phenological observation data from 10 agricultural meteorological stations in Heilongjiang Province, China, model simulation was employed to determine the control mechanism of leaf unfolding of P. simonii. Furthermore, the predicting effects of nine phenology-simulating models for P. simonii leaf unfolding were evaluated and the distribution characteristics of P. simonii leaf unfolding in China in 2015 were simulated. The results show that P. simonii leaf unfolding is sensitive to air temperature;consequently, climate warming could advance the P. simonii leaf unfolding process. The phenological model based on air temperature could be better suited for simulating P. simonii leaf unfolding, with 76.7% of the calibration data of absolute error being less than three days. The performance of the models based solely on forcing requirements was found superior to that of the models incorporating chilling. If it was imperative that the chilling threshold is reached, the south of the Yunnan, Guangdong, and Guangxi provinces would be unsuitable for planting P. simonii. In this regard, the phenology model based on the chilling threshold as necessary condition was indicated a more reasonable model for the distribution characteristics of P. simonii leaf unfolding.

关键词： Phenology Populus simonii Tree Distribution Leaf Unfolding Regional Cli-mate Warming

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CRISPR/Cas9基因编辑技术在食用菌中的应用现状

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生物工程学报 2024年第4期40卷 988-1001页

作者：李荣平李荣春云南菌视界生物科技有限公司云南省级(珍稀食用菌)企业工程技术中心昆明市级(食用菌)企业工程技术中心云南昆明650200 云南省张劲松专家工作站云南昆明650200 云南(昆明)张劲松药用真菌专家工作站云南昆明650200 云南农业大学食用菌研究所云南昆明650201

CRISPR/Cas9基因编辑系统是一项通用的基因修饰技术,是植物、动物、真菌以及微生物的功能基因和遗传育种研究的重要技术。本文介绍了该技术在食用真菌的基因研究和遗传育种中的应用现状,包括Cas9和sgRNA的递送和表达策略、遗传转化方法... 详细信息

CRISPR/Cas9基因编辑系统是一项通用的基因修饰技术,是植物、动物、真菌以及微生物的功能基因和遗传育种研究的重要技术。本文介绍了该技术在食用真菌的基因研究和遗传育种中的应用现状,包括Cas9和sgRNA的递送和表达策略、遗传转化方法、突变体筛选以及DNA双链断裂后的靶位点的修复策略。同时总结了该技术在食用菌中应用所面临的主要问题及其优化策略,并结合个人研究背景展望了其未来在食用菌研究中的应用价值。

关键词： CRISPR/Cas9 食用菌基因编辑 Cas9-RNP

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真空蒸发制备Zn(S,Se)薄膜及其性能研究

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太阳能学报 2006年第10期27卷 982-985页

作者：李蓉萍荣利霞内蒙古大学理工学院呼和浩特010021 中国科学院高能物理所北京100039

利用真空蒸发技术在ZnS粉末中掺入不同比例的Se粉末作为源材料,选择合适的工艺条件在玻璃衬底上获得了性能稳定的Zn(S,Se)薄膜,薄膜为纤锌矿结构,具有沿[002]晶向的择优取向。Zn(S,Se)薄膜为n型材料呈高阻状态,在可见光范围内具有良好... 详细信息

利用真空蒸发技术在ZnS粉末中掺入不同比例的Se粉末作为源材料,选择合适的工艺条件在玻璃衬底上获得了性能稳定的Zn(S,Se)薄膜,薄膜为纤锌矿结构,具有沿[002]晶向的择优取向。Zn(S,Se)薄膜为n型材料呈高阻状态,在可见光范围内具有良好的透过率。随Se成分的增加薄膜由无色透明变为橘黄色透明,吸收限向长波方向移动。

关键词： Zn(S,Se)薄膜薄膜制备真空蒸发性能研究

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用于牛粪液厌氧消化的推流式和完全混合式反应器性能研究

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农业工程学报 2007年第9期23卷 186-190页

作者：李荣平李秀金 Shulin Chen 北京化工大学环境科学与工程系北京100029 Department of Biological System Engineering Washington State University

对推流式反应器（PFR）和完全混合式反应器（CSTR）用于牛粪液厌氧消化的性能进行了比较研究.在中温35℃下,对进料浓度40、80和128 g/L的3种牛粪液分别进行了试验.结果表明,CSTR比PFR具有更好的去除VS（挥发性固体）和产生物气的性能,... 详细信息

对推流式反应器（PFR）和完全混合式反应器（CSTR）用于牛粪液厌氧消化的性能进行了比较研究.在中温35℃下,对进料浓度40、80和128 g/L的3种牛粪液分别进行了试验.结果表明,CSTR比PFR具有更好的去除VS（挥发性固体）和产生物气的性能,在进料浓度为40、80和128 g/L时,CSTR中VS去除率比PFR中分别提高了17.4%～21.5%,3.9%～21.5%和0.5%～5.3%.在最优进料浓度80 g/L下,CSTR获得了最高的容积产气率和单位VS产气量,比PFR中提高了9.8%～25.9%.研究认为,CSTR中的搅拌作用加强了微生物与物料之间物质的传递,提高了降解去除有机物和产生物气的消化效果.因此,在牛粪液厌氧消化时,推荐使用CSTR反应器.

关键词：牛粪液厌氧消化推流式反应器完全混合式反应器性能比较进料浓度

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基础设施PPP项目脆弱性评估方法

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系统工程理论与实践 2016年第3期36卷 613-622页

作者：季闯黄伟袁竞峰李启明李荣平东南大学土木工程学院南京210096 三江学院南京210012 首创南京投资管理有限公司南京210014

为获知一个PPP(public private partnership)项目暴露于外部环境不利影响的程度、抵抗干扰和自我调节恢复的能力,提出一种:PPP项目脆弱性评估方法.将PPP项目看作一个由功能、性能、可盈利性、可维护性、可运营性和可持续性6个维度组成... 详细信息

为获知一个PPP(public private partnership)项目暴露于外部环境不利影响的程度、抵抗干扰和自我调节恢复的能力,提出一种:PPP项目脆弱性评估方法.将PPP项目看作一个由功能、性能、可盈利性、可维护性、可运营性和可持续性6个维度组成的系统,引入可靠性理论,根据识别出的27个脆弱性影响因素与各维度之间的关系,推导出PPP项目系统可靠性函数,通过评价系统各维度失效程度确定系统的可靠度,计算结果反映了PPP项目脆弱性的大小.将PPP项目脆弱性评估模型应用于某大桥PPP项目脆弱性评估,结果显示该项目具有较大的脆弱性,应加强项目的盈利和可持续性方面的管理.

关键词：政府与社会资本合作(PPP) 脆弱性可靠性理论评估方法

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