Involvement of NAC transcription factor NaNAC29 in Alternaria alternata resistance and leaf senescence in Nicotiana attenuata

作     者：Lan Ma Rongping Li Luoyan Ma Na Song Zhen Xu Jinsong Wu Lan Ma;Rongping Li;Luoyan Ma;Na Song;Zhen Xu;Jinsong Wu

作者机构：Yunnan Key Laboratory for Wild Plant ResourcesKunming Institute of BotanyChinese Academy of SciencesLanhei Road 132650201KunmingChina University of Chinese Academy of ScienceBeijing 100049China 

出 版 物：《Plant Diversity》 (植物多样性（英文版）)

年 卷 期：2021年第43卷第6期

页      面：502-509页

核心收录：

学科分类：0710[理学-生物学] 0830[工学-环境科学与工程（可授工学、理学、农学学位）] 09[农学] 0904[农学-植物保护] 0901[农学-作物学] 090401[农学-植物病理学] 0902[农学-园艺学] 090402[农学-农业昆虫与害虫防治] 0713[理学-生态学] 

基　　金：supported by CAS“Light of West China”Program and NSFC grant(No.31700231)to LM 

主　　题：NaNAC29 Defensin-like protein 1 Tobacco Brown spot disease Aging 

摘      要：NAC-LIKE,ACTIVATED BY AP3/PI(NAP)is a NAC transcription factor regulating leaf senescence in Arabidopsis *** wild tobacco Nicotiana attenuata,a nuclear localized NAC transcription factor NaNAC29 was identified to be highly elicited after inoculation of Alternaria alternata,a notorious necrotic fungus on tobacco *** NaNAC29 possesses similar tertiary structure to NAP with 60%amino acid ***,it remains unknown the role of NaNAC29 in plant defense responses to *** and leaf senescence in *** this paper,Defensin-like protein 1(NaDLP1)was highly induced in *** after *** inoculation and bigger lesions were developed in NaDLP1-silenced ***,***-induced NaDLP1 was reduced by 76%in VIGS NaNAC29 plants and by 61%in JA deficient irAOC plants at 3 days post *** regulation of NaDLP1 expression by NaNAC29 was clearly independent on JA pathway,since exogenous methyl jasmonate treatment could not complement the induction levels of NaDLP1 in NaNAC29-silenced plants to the levels in WT ***,the expression of NaNAC29 was low expressed in young leaves but highly in senescent leaves and darktreated ***29-silenced plants,which were generated by virus-induced gene silencing(VIGS NaNAC29),showed delayed senescence *** addition,constitutive over-expression of NaNAC29 in *** could rescue the delayed-senescence phenotype of nap and caused precocious leaf senescence of wild-type Col-0 *** the data above demonstrate that NaNAC29 is a NAP homolog in *** participating in the defense responses to *** by regulation of a defensin protein NaDLP1 and promoting leaf senescence.