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High auxin stimulates callus through SDG8-mediated histone H3K36 methylation in Arabidopsis

Journal of Integrative Plant Biology 2022年第12期64卷 2425-2437页

作者： Jun Ma Qiang Li Lei Zhang Sen Cai Yuanyuan Liu Juncheng Lin rongfeng huang Yongqiang Yu Mingzhang Wen Tongda Xu College of Life Sciences Fujian Agriculture and Forestry UniversityFuzhou 350002China Plant Synthetic Biology Center FAFU-UCR Joint Center for Horticultural Biology and MetabolomicsHaixia Institute of Science and TechnologyFujian Agriculture and Forestry UniversityFuzhou 350002China Shanghai Center for Plant Stress Biology CAS Center for Excellence in Molecular Plant SciencesChinese Academy of SciencesShanghai 201602China Basic Forestry and Proteomics Research Center Haixia Institute of Science and TechnologyFujian Agricuture and Forestry UniversityFuzhou 350002China Frontiers Science Center for Synthetic Biology(Ministry of Education) Tianin UniversityTianjin 300072China

Callus induction,which results in fate transition in plant cells,is considered as the first and key step for plant regeneration.This process can be stimulated in different tissues by a callus-inducing medium(CIM),which contains a high concentration of phytohormone auxin.Although a few key regulators for callus induction have been identified,the multiple aspects of the regulatory mechanism driven by high levels of auxin still need further investigation.Here,we find that high auxin induces callus through a H3 K36 histone methylation-dependent mechanism,which requires the methyltransferase SET DOMAIN GROUP 8(SDG8).During callus induction,the increased auxin accumulates SDG8 expression through a TIR1/AFBs-based transcriptional regulation.SDG8 then deposits H3 K36 me3 modifications on the loci of callus-related genes,including a master regulator WOX5 and the cell proliferation-related genes,such as CYCB1.1.This epigenetic regulation in turn is required for the transcriptional activation of these genes during callus formation.These findings suggest that the massive transcriptional reprogramming for cell fate transition by auxin during callus formation requires epigenetic modifications including SDG8-mediated histone H3 K36 methylation.Our results provide insight into the coordination between auxin signaling and epigenetic regulation during fundamental processes in plant development.

关键词： auxin callus formation epigenetic regulation H3K36 methylation SDG8 TIR1/AFBs

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Circulating exosome-like vesicles of humans with nondiabetic obesity impaired isletβ-cell proliferation,which was associated with decreased Omentin-1 protein cargo

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Genes & Diseases 2022年第4期9卷 1099-1113页

作者： Qian Ge Xinxin Xie Xiangjun Chen rongfeng huang Cheng-Xue Rui Qianna Zhen Renzhi Hu Min Wu Xiaoqiu Xiao Xi Li Department of Endocrinology The First Affiliated Hospital of Chongqing Medical UniversityChongqing 400016PR China The Biology Science Institutes Chongqing Medical UniversityChongqing 400016PR China The Chongqing Key Laboratory of Translational Medicine in Major Metabolic Diseases The First Affiliated Hospital of Chongqing Medical UniversityChongqing 400016PR China de Duve Institute Catholic University of LouvainBrussels 1200Belgium

The regulation ofβ-cell mass in the status of nondiabetic obesity remains not well understood.We aimed to investigate the role of circulating exosome-like vesicles(ELVs)isolated from humans with simple obesity in the regulation of isletβ-cell mass.Between June 2017 and July 2019,81 subjects with simple obesity and 102 healthy volunteers with normal weight were recruited.ELVs were isolated by ultra-centrifugation.The proliferations ofβ-cells and islets were measured by 5-ethynl-2′-deoxyuridine(EdU).Protein components in ELVs were identified by Quantitative Proteomic Analysis and verified by Western blot and ELISA.The role of specific exosomal protein was analyzed by gain-of-function approach in ELVs released by 3T3-L1 preadipocytes.Circulating ELVs from subjects with simple obesity inhibitedβ-cell proliferation in vitro without affecting its apoptosis,secretion,and inflammation.The protein levels of Rictor and Omentin-1 were downregulated in circulating ELVs from subjects with simple obesity and associated with the obesity-linked pathologic conditions.The ELV-carried Omentin-1 and Omentin-1 protein per se were validated to increaseβ-cell proliferation and activate Akt signaling pathway.Moreover,Omentin-1 in ELVs was downregulated by insulin.The circulating ELVs may act as a negative regulator forβ-cell mass in nondiabetic obesity through inhibitingβ-cell proliferation.This effect was associated with downregulated Omentin-1 protein in ELVs.This newly identified ELV-carried protein could be a mediator linking insulin resistance to impairedβ-cell proliferation and a new potential target for increasingβ-cell mass in obesity and T2DM.

关键词： β-Cell Exosome Obesity Proliferation Type 2 diabetes mellitus

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The chloroplast-localized protein LTA1 regulates tiller angle and yield of rice

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The Crop Journal 2022年第4期10卷 952-961页

作者： Xiaowu Pan Yongchao Li Haiwen Zhang Wenqiang Liu Zheng Dong Licheng Liu Sanxiong Liu Xinnian Sheng Jun Min rongfeng huang Xiaoxiang Li Rice Research Institute Hunan Academy of Agricultural SciencesChangsha 410125HunanChina Biotechnology Research Institute Chinese Academy of Agricultural SciencesBeijing 100081China

Plant architecture strongly influences rice grain yield.We report the cloning and characterization of the LTA1 gene,which simultaneously controls tiller angle and yield of rice.LTA1 encodes a chloroplastlocalized protein with a conserved YbaB DNA-binding domain,and is highly expressed in photosynthetic tissues including leaves and leaf sheaths.Disrupting the function of LTA1 leads to large tiller angle and yield reduction of rice.LTA1 affects the gravity response by mediating the distribution of endogenous auxin,thereby regulating the tiller angle.An lta1 mutant showed abnormal chloroplast development and decreased chlorophyll content and photosynthetic rate,in turn leading to reduction of rice yield.Our findings shed light on the genetic basis of tiller angle and provide a potential gene resource for the improvement of plant architecture and rice yield.

关键词： Rice Yield Tiller angle Gravitropism Chloroplast development

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Hippocampal overexpression of TREM2 ameliorates high fat diet induced cognitive impairment and modulates phenotypic polarization of the microglia

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Genes & Diseases 2022年第2期9卷 401-414页

作者： Min Wu Maolin Liao rongfeng huang Chunxiu Chen Tian Tian Hongying Wang Jiayu Li Jibin Li Yuxiang Sun Chaodong Wu Qifu Li Xiaoqiu Xiao Department of Endocrinology The First Affiliated Hospital of Chongqing Medical UniversityChongqing 400016PR China The Chongqing Key Laboratory of Translational Medicine in Major Metabolic Diseases The First Affiliated Hospital of Chongqing Medical UniversityChongqing 400016PR China Department of Nutrition and Food Hygiene School of Public Health and ManagementChongqing Medical UniversityChongqing 400016PR China Department of Neurology Affiliated Hospital of Guizhou Medical UniversityGuiyangGuizhou 550004PR China Department of Nutrition and Food Science Texas A&M UniversityCollege StationTX 77843USA

Type 2 diabetes mellitus(T2DM)and Alzheimer's disease(AD)share several common pathophysiological features.Rare variants of triggering receptor expressed on myeloid cells 2(TREM2)increase the risk of developing AD,suggesting the involvement of TREM2 and innate immunity in AD development.It is still unknown whether TREM2 is related to cognitive impairment in T2DM.Here,we investigated the effects of the hippocampal overexpression of TREM2 on cognitive in long-term high-fat diet(HFD)-fed mice.Male C57BL/6J mice were maintained on HFD for 50 weeks.TREM2 was overexpressed in the hippocampus 36 weeks after HFD feeding using adeno-associated virus vector(AAV)-mediated gene delivery.The results showed that the HFD feeding induced rapid and persistent weight gain,glucose intolerance and significant impairments in learning and memory.Compared with AAV-con,AAV-TREM2 significantly ameliorated cognitive impairment without altering body weight and glucose homeostasis in HFD mice.The overexpression of TREM2 upregulated the synaptic proteins spinophilin,PSD95 and synaptophysin,suggesting the improvement in synaptic transmission.Dendritic complexity and spine density in the CA1 region were rescued after TREM2 overexpression.Furthermore,TREM2 markedly increased the number of iba-1/Arg-1-positive microglia in the hippocampus,suppressed neuroinflammation and microglial activation.In sum,hippocampal TREM2 plays an important role in improving HFD-induced cognitive dysfunction and promoting microglial polarization towards the M2 anti-inflammatory phenotype.Our study also suggests that TREM2 might be a novel target for the intervention of obesity/diabetes-associated cognitive decline.

关键词： Diabetes Microglial polarization Neurodegeneration Neuroinflammation Obesity TREM2

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Ethylene Response Factor TERF1 Enhances Glucose Sensitivity in Tobacco through Activating the Expression of Sugar-related Genes

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Journal of Integrative Plant Biology 2009年第2期51卷 184-193页

作者： Ang Li Zhijin Zhang Xue-Chen Wang rongfeng huang National Laboratory of Plant Physiology and Biochemistry College of Biological Sciences China Agricultural University Beijing 100094 China Biotechnology Research Institute the Chinese Academy of Agricultural Sciences Beijing 100081 China National Key Facility of Crop Gene Resources and Genetic Improvement Beijing 100081 China National Center for Plant Gene Research (Beijing) Beijing 100081 China

Ethylene response factor （ERF） proteins are important plant-specific transcription factors. Increasing evidence shows that ERF proteins regulate plant pathogen resistance, abiotic stress response and plant development through interaction with different stress responsive pathways. Previously, we revealed that overexpression of TERF1 in tobacco activates a cluster gene expression through interacting with GCC box and dehydration responsive element （DRE）, resulting in enhanced sensitivity to abscisic acid （ABA） and tolerance to drought, and dark green leaves of mature plants, indicating that TERF1 participates in the integration of ethylene and osmotic responses. Here we further report that overexpression of TERF1 confers sugar response in tobacco. Analysis of the novel isolated tomato TERF1 promoter provides information indicating that there are many cis-acting elements, including sugar responsive elements （SURE） and W box, suggesting that TERF1 might be sugar inducible. This prediction is confirmed by results of reverse transcription-polymerase chain reaction amplification, indicating that transcripts of TERF1 are accumulated in tomato seedlings after application of glucose. Further investigation indicates that the expression of TERF1 in tobacco enhances sensitivity to glucose during seed germination, root and seedling development, showing a decrease of the fresh weight and root elongation under glucose treatment. Detailed investigations provide evidence that TERF1 interacts with the sugar responsive cis-acting element SURE and activates the expression of sugar response genes, establishing the transcriptional regulation of TERF1 in sugar response. Therefore, our results deepen our understanding of the glucose response mediated by the ERF protein TERF1 in tobacco.

关键词： Ethylene response factor protein TERF1 glucose sugar responsive gene tobacco

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Abscisic Acid Antagonizes Ethylene Production through the ABI4-Mediated Transcriptional Repression of ACS4 and ACS8 in Arabidopsis

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Molecular Plant 2016年第1期9卷 126-135页

作者： Zhijun Dong Yanwen Yu Shenghui Li Juan Wang Saijun Tang rongfeng huang College of Biological Sciences China Agricultural University Beijing 100193 China plant Molecular Biology Department Biotechnology Research Institute Chinese Academy of Agricultural Sciences Beijing 100081 China National Key Facility of Crop Gene Resources and Genetic Improvement Beijing 100081 China These authors contributed equally to this article

Increasing evidence has revealed that abscisic acid （ABA） negatively modulates ethylene biosynthesis, although the underlying mechanism remains unclear. To identify the factors involved, we conducted a screen for ABA-insensitive mutants with altered ethylene production in Arabidopsis. A dominant allele of ABI4, abi4-152, which produces a putative protein with a 16-amino-acid truncation at the C-terminus of ABI4, reduces ethylene production. By contrast, two recessive knockout alleles of ABI4, abi4-102 and abi4-103, result in increased ethylene evolution, indicating that ABI4 negatively regulates ethylene produc- tion. Further analyses showed that expression of the ethylene biosynthesis genes ACS4, ACSS, and AC02 was significantly decreased in abi4-152 but increased in the knockout mutants, with partial dependence on ABA. Chromatin immunoprecipitation-quantitative PCR assays showed that ABI4 directly binds the pro- moters of these ethylene biosynthesis genes and that ABA enhances this interaction. A fusion protein containing the truncated ABI4-152 peptide accumulated to higher levels than its full-length counterpart in transgenic plants, suggesting that ABI4 is destabilized by its C terminus. Therefore, our results demon- strate that ABA negatively regulates ethylene production through ABI4-mediated transcriptional repression of the ethylene biosynthesis genes ACS4 and ACS8 in Arabidopsis.

关键词： ABA ABI4 ethylene biosynthesis stress response transcriptional regulation

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2018-2022年南京市公众预防接种相关舆情特征分析

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中国公共卫生 2024年第3期40卷 367-370页

作者：黄琦敏梁亚琼周荣锋李媛媛南京市疾病预防控制中心江苏210003通信作者:烫亚琼1

目的分析南京市公众预防接种相关舆情特征。方法从南京12 345政务热线平台和南京12 320健康服务平台收集2018-2022年预防接种相关工单1 525件,收集个案的工单编号、诉求时间、诉求渠道、诉求内容的详细信息,将信息录入EXCEL 2010软件... 详细信息

目的分析南京市公众预防接种相关舆情特征。方法从南京12 345政务热线平台和南京12 320健康服务平台收集2018-2022年预防接种相关工单1 525件,收集个案的工单编号、诉求时间、诉求渠道、诉求内容的详细信息,将信息录入EXCEL 2010软件形成数据库后分类汇总分析。结果 2018-2022年,南京市疾病预防控制中心共受理预防接种相关工单1 525件,平均月工单量为25.42件。2018年工单件数位列第1的为7月47件,占比78.33%;2019年工单件数位列第1的为6月和11月各7件,分别占比14.29%;2020年工单件数位列第1的为2月11件,占比20.00%;2021年工单件数位列第1的为8月227件,占比32.24%;2022年工单件数位列第1的为12月165件,占比25.11%。诉求内容涉及单品种疫苗种类位列前3的为新冠病毒疫苗1 128件,人乳头瘤病毒(HPV)疫苗103件和狂犬疫苗72件。2018-2022年新上市的疫苗中,带状疱疹疫苗85.71%的诉求内容为接种门诊地点,接种时间,预约接种流程,接种需要携带材料。新冠病毒疫苗此类诉求内容占比47.52%。结论疫苗质量安全问题,重大突发事件导致免疫服务暂缓,大规模接种的疫苗免疫程序调整可导致预防接种相关舆情数量短期出现较大波动。新上市疫苗主要诉求内容为如何接种到疫苗。

关键词：预防接种 12345政务服务热线 12320卫生热线舆情

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“双碳”目标下皮革边角料的二次设计与应用研究

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中国皮革 2024年第3期53卷 24-26,32页

作者：黄立中郑荣锋成都东软学院四川成都611844 四川长虹电器股份有限公司四川绵阳621000

近年来,随着“双碳”战略的深入推进,传统制造行业需要不断推动整体的全绿转型,不断提升专业能力,在绿色发展的框架下,在低碳转型的途径上迈出新的步伐。本文对“双碳”目标下皮革边角料的二次设计与应用方面进行了探讨,助力皮革行业实... 详细信息

近年来,随着“双碳”战略的深入推进,传统制造行业需要不断推动整体的全绿转型,不断提升专业能力,在绿色发展的框架下,在低碳转型的途径上迈出新的步伐。本文对“双碳”目标下皮革边角料的二次设计与应用方面进行了探讨,助力皮革行业实现节能减排、清洁化生产和实现碳达峰、碳中和。

关键词：双碳皮革边角料设计和应用

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Knockdown of hepatocyte Perilipin-3 mitigates hepatic steatosis and steatohepatitis caused by hepatocyte CGI-58 deletion in mice

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Journal of Molecular Cell Biology 2022年第8期14卷 43-52页

作者： Xinyu Bao Xiaogen Ma rongfeng huang Jianghui Chen Haoran Xin Meiyu Zhou Lihua Li Shifei Tong Qian Zhang Guanghou Shui Fang Deng Liqing Yu Min-Dian Li Zhihui Zhang Department of Cardiovascular Medicine Center for Circadian Metabolism and Cardiovascular DiseaseSouthwest HospitalArmy Medical UniversityChongqing 400038China Department of Cardiology The Third Affiliated Hospital of Chongqing Medical UniversityChongqing 401120China Department of General Medicine Southwest HospitalArmy Medical UniversityChongqing 400038China State Key Laboratory of Molecular Developmental Biology Institute of Genetics and Developmental BiologyChinese Academy of SciencesBeĳing 100101China Department of Pathophysiology College of High Altitude Military MedicineArmy Medical UniversityChongqing 400038China Key Laboratory of Extreme Environmental Medicine Ministry of Education of ChinaChongqing 400038China Key Laboratory of High Altitude Medicine PLAChongqing 400038China Division of Endocrinology Diabetes and NutritionDepartment of MedicineUniversity of Maryland School of MedicineBaltimoreMD 21201USA

Comparative gene identification-58(CGI-58),also known asα/βhydrolase domain containing 5,is the co-activator of adipose triglyceride lipase that hydrolyzes triglycerides stored in the cytosolic lipid droplets.Mutations in CGI-58 gene cause Chanarin-Dorfman syndrome(CDS),an autosomal recessive neutral lipid storage disease with ichthyosis.The liver pathology of CDS manifests as steatosis and steatohepatitis,which currently has no effective treatments.Perilipin-3(Plin3)is a member of the Perilipin-ADRP-TIP47 protein family that is essential for lipid droplet biogenesis.The objective of this study was to test a hypothesis that deletion of a major lipid droplet protein alleviates fatty liver pathogenesis caused by CGI-58 deficiency in hepatocytes.Adult CGI-58-floxed mice were injected with adeno-associated vectors simultaneously expressing the Cre recombinase and microRNA against Plin3 under the control of a hepatocyte-specific promoter,followed by high-fat diet feeding for 6 weeks.Liver and blood samples were then collected from these animals for histological and biochemical analysis.Plin3 knockdown in hepatocytes prevented steatosis,steatohepatitis,and necroptosis caused by hepatocyte CGI-58 deficiency.Our work is the first to show that inhibiting Plin3 in hepatocytes is sufficient to mitigate hepatocyte CGI-58 deficiency-induced hepatic steatosis and steatohepatitis in mice.

关键词： lipid droplet lipolysis rare human disease nonalcoholic fatty liver disease nonalcoholic steatohepatitis Chanarin-Dorfman syndrome

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牦牛FABP3基因克隆及组织表达谱分析

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西北农林科技大学学报（自然科学版） 2024年第1期52卷 1-8页

作者：戴荣凤黄纯扎老路建卫唐月琴梁春年中国农业科学院兰州畜牧与兽药研究所甘肃省牦牛繁育工程重点实验室甘肃兰州730050 农业农村部青藏高原畜禽遗传资源育种重点实验室甘肃兰州730050 合作市佐盖多玛乡畜牧站甘肃甘南747000 合作市农畜产品质量安全检测检验中心甘肃甘南747000

【目的】分析牦牛心肌脂肪酸结合蛋白3(FABP3)基因的结构和功能,并检测其在成年牦牛和胎牛中的表达水平,为探究该基因在牦牛育种中的生物学功能提供理论参考。【方法】以美仁牦牛的心脏组织为试验材料,PCR扩增FABP3基因,对得到的编码区... 详细信息

【目的】分析牦牛心肌脂肪酸结合蛋白3(FABP3)基因的结构和功能,并检测其在成年牦牛和胎牛中的表达水平,为探究该基因在牦牛育种中的生物学功能提供理论参考。【方法】以美仁牦牛的心脏组织为试验材料,PCR扩增FABP3基因,对得到的编码区序列(CDS)进行生物信息学分析;利用实时荧光定量PCR(RT-qPCR),检测FABP3基因在成年牦牛和胎牛心脏、肝脏、脾脏、肺脏、肾脏及肌肉组织的表达水平。【结果】牦牛FABP3基因编码区序列长度为402 bp,编码133个氨基酸;蛋白质的高级结构主要是β-转角和延伸链;牦牛FABP3蛋白不存在跨膜区域和信号肽,属于具有一定亲水性的稳定蛋白;牦牛FABP3基因CDS区核苷酸及其编码氨基酸序列的同源性分析显示,FABP3基因在牛属动物中具有一定的保守性;系统进化树分析表明,牦牛与野牦牛和瘤牛的亲缘关系最近,与鸡的亲缘关系最远。RT-qPCR结果显示,FABP3基因在成年牦牛和胎牛的心脏、肝脏、脾脏、肺脏、肾脏及肌肉组织中均有表达,但在胎牛肝脏、脾脏、肾脏和肌肉中的表达水平显著或极显著高于成年牦牛,在成年牦牛肺脏中的表达水平极显著高于胎牛。【结论】克隆了牦牛FABP3基因,探究了FABP3基因在牦牛中的组织表达规律,为进一步研究该基因在牦牛脂肪沉积中的作用提供了基础数据。

关键词：美仁牦牛 FABP3 基因克隆生物信息学组织表达谱

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