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Hepatobiliary & Pancreatic Diseases International 2022年第2期21卷 202-204页

作者： Bao Dong rong-rong wu The First Affiliated Hospital of USTC Division of Life Sciences and MedicineUniversity of Science and Technology of ChinaHefei 230031China

To the Editor:Pancreatic cancer (PC) is one of the most deadly cancers due to the difficulty of its early *** patients have developed an unresectable PC when diagnosed [1].According to GLOBOCAN2018 estimates,PC ranked the 11th most common cancer in the world counting 458 918 new cases and causing 432 242 deaths(4.5%of all deaths caused by cancer) in 2018 [2].

关键词： patients cancer diabetes

Immunomodulative effects of mesenchymal stem cells derived from human embryonic stem cells in vivo and in vitro

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2011年第1期12卷 18-27页

作者： Zhou TAN Zhong-yuan SU rong-rong wu Bin GU Yu-kan LIU Xiao-li ZHAO Ming ZHANG Institute of Cell Biology and Genetics College of Life Sciences Zhejiang University Hangzhou 310058 China Institute of Developmental and Regenerative Biology College of Life and Environmental Sciences Hangzhou Normal University Hangzhou 310036 China

Objective: Human embryonic stem cells (hESCs) have recently been reported as an unlimited source of mesenchymal stem cells (MSCs).The present study not only provides an identical and clinically compliant MSC source derived from hESCs (hESC-MSCs),but also describes the immunomodulative effects of hESC-MSCs in vitro and in vivo for a carbon tetrachloride (CCl4)-induced liver inflammation ***: Undifferentiated hESCs were treated with Rho-associated kinase (ROCK) inhibitor and induced to fibroblast-looking *** cells were tested for their surface markers and multilineage differentiation *** more,we analyzed their immune characteristics by mixed lymphocyte reactions (MLRs) and animal ***: hESC-MSCs show a homogenous fibroblastic morphology that resembles bone marrow-derived MSCs (BM-MSCs).The cell markers and differentiation potential of hESC-MSCs are also similar to those of *** their original cells,hESC-MSCs possess poor immunogenicity and can survive and be engrafted into a xenogenic immunocompetent ***: The hESC-MSCs demonstrate strong inhibitory effects on lymphocyte proliferation in vitro and anti-inflammatory infiltration properties in *** study offers information essential to the applications of hESC-MSC-based therapies and evidence for the therapeutic mechanisms of action.

关键词： Human embryonic stem cells Mesenchymal stem cells Differentiation Immunomodulative effects

Tongxinluo promotes mesenchymal stem cell tube formation in vitro

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2011年第8期12卷 644-651页

作者： Xin-yang HU Wen-xia WANG Min-jia YU Xian-bao LIU rong-rong wu Feng GAO Xin HUANG Jiang CAO Xiao-jie XIE Jian-an WANG Cardiovascular Key Lab of Zhejiang Province the Second Affiliated Hospital School of Medicine Zhejiang University Hangzhou 310009 China Department of Cardiology the Second Affiliated Hospital School of Medicine Zhejiang University Hangzhou 310009 China

Objective： To study whether Tongxinluo （TXL） can induce angiogenesis in bone marrow mesenchymal stem cells （MSCs）, and to investigate the underlying mechanism. Methods： Bone marrow MSCs were obtained from male Sprague-Dawley rats. We established an angiogenesis model in vitro via matrigel experiment. MSCs were seeded on matrigel coated 24-well plates, and treated by TXL 50 and 100 mg/L. After 24 h, we observed the tube formations of MSCs in the matrigel. Cell migration ability was examined by wound scratch test and transwell assay. Expressions of vascular endothelial growth factor （VEGF）, fetal liver kinase-1 （FIk-1）, matrix metalloproteinase-2 （MMP-2）, MMP-9, and tissue inhibitor of metalloproteinase-2 （TIMP-2） were analyzed at the protein level by Western blot. Gelatin zymography assay was applied to investigating the MSC paracrine abilities of pro-MMP-2 and activated MMP-2. Results： TXL promoted MSC tube formation in matrigel. The ratio of TXL 100 mg/L treated-MSC tubular length was increased 3.04-fold compared to the control group （P〈0.05）. Scratch test and transwell assay showed that TXL could improve the cell migration ability of MSCs. Western blot experiments showed that TXL promoted MSC synthesis of MMP-2, but it had no influence on the expressions of MMP-9 and TIMP-2. This effect was confirmed by gelatin zymography assay, which showed that TXL increased MSC secretion of pro-MMP-2 and activated MMP-2. VEGF expression of TXL treated-MSCs was increased compared to the control group. The expression of FIk-1 was not different among the groups. Conclusions： This study demonstrates that TXL can promote the tube formation of MSCs, and the underlying mechanisms are associated with increased migration ability of MSCs and the up-regulation of MMP-2 and VEGF expressions.

关键词： Bone marrow mesenchymal stem cells Tongxinluo Angiogenesis Migration Tube formation

Pulmonary artery aneurysm protruding into the bronchus as an endobronchial mass:A case report

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World Journal of Clinical Cases 2023年第28期11卷 6792-6796页

作者： Min Li Wen-Ye Zhu rong-rong wu Le Wang Meng-Ting Mo Shi-Nan Liu Dong-Yi Zhu Zhuang Luo Department of Respiratory and Critical Care Medicine The First Affiliated Hospital of Kunming Medical UniversityKunming 650032Yunnan ProvinceChina Department of Pharmacy The First Affiliated Hospital of Kunming Medical UniversityKunming 650032Yunnan ProvinceChina Department of Radiology The First People’s Hospital of Yunnan Province/Affiliated Hospital of Kunming University of Science and TechnologyKunming 650034Yunnan ProvinceChina

BACKGROUND Pulmonary artery(PA)aneurysms are usually diagnosed radiographically and present as small or large lesions resembling inflammation or a neoplasm on chest *** has rarely been reported as an endobronchial *** SUMMARY We report the case of a 64-year-old man who presented with recurrent *** revealed a tumorous protrusion blocking the right middle lobe bronchus,which was confirmed to be a PA aneurysm using endobronchial ultrasound bronchoscopy and computed tomography *** Although endobronchial PA aneurysms are rare,bronchoscopists need to add this lesion to the list of endobronchial masses for which a biopsy is to be assiduously avoided.

关键词： Pulmonary artery aneurysm Endobronchial mass Endobronchial ultrasound bronchoscopy Computed tomography angiography Case report

Metabolism alternation of schisandra chinensis in carbon tetrachloride-intoxicated rats and patients with advanced hepatocellular carcinoma

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中国药理学与毒理学杂志 2018年第4期32卷 314-314页

作者： rong-rong wu Zhi-yong XIAO Xiao-rui ZHANG Feng LIU Wen-xia ZHOU Yong-xiang ZHANG State Key Laboratory of Toxicology and Medical Countermeasures Department of NeuroimmunopharmacologyInstitute of Pharmacology and Toxicology Department of Pharmacy 302 Hospital of People's Liberation Army

OBJECTIVE To study the pharmacokinetics change of schisandra chinensis under the pathological condition of liver dysfunction for safe and rational use of herbal *** The metabolism of four effective lignans from schisandra chinensis(SC),schisandrin,schisantherin A,deoxyshisandrin and γ-schisandrin was studied using microsomes from patients with advanced *** *** situ intestinal and hepatic perfusions were conducted to clarify the contributions from impairments of gut and liver on the pharmacokinetics of the four schisandra lignans in *** *** metabolism in rat and human liver microsomes and transport in Caco-2 monolayer cell model were studied to reveal the key factors for the in vivo disposition of the four *** When SC alcoholic extract was orally administrated to CCl4-intoxicated rat for a short term(4 d),the pharmacokinetics of four active SC lignans was significantly changed while its hepatoprotective effect was not obviously *** plasma concentrations of the four schisandra lignans were *** elevated compared with the *** Cmax,AUC and MRT were all increased or prolonged *** while parameter CLz/F was obviously reduced in rat pretreated with *** hepatic perfusion study and liver microsomes incubation,it was found that the hepatic metabolism of the four lignans was markedly decreased mainly due to the activity reduction of multiple CYP450 isoenzymes involved the metabolism,which,eventually,might lead to the alternation of their pharmacokinetic profiles in CCl4-intoxicated rats or patients with advanced hepatocellular *** The *** studies of SC components in pathological situation of liver dysfunction are expected to provide useful data for rational and safe application of SC preparations in clinic or further pharmacological and toxicological research.

关键词：五味子肝功能治疗方法临床分析

Inhibiting ceramide synthase 5 expression in microglia decreases neuroinflammation after spinal cord injury

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Neural Regeneration Research 2025年第10期20卷 2955-2968页

作者： Wei Zhang Yubao Lu Ruoqi Shen Yingjie wu Chenrui Liu Xingxing Fang Liangming Zhang Bin Liu Limin rong Department of Spine Surgery The Third Affiliated HospitalSun Yat-sen UniversityGuangzhouGuangdong ProvinceChina National Medical Products Administration(NMPA)Key Laboratory for Quality Research and Evaluation of Cell Products GuangzhouGuangdong ProvinceChina Guangdong Provincial Center for Quality Control of Minimally Invasive Spine Surgery GuangzhouGuangdong ProvinceChina Guangdong Provincial Center for Engineering and Technology Research of Minimally Invasive Spine Surgery GuangzhouGuangdong ProvinceChina

Microglia,the resident monocyte of the central nervous system,play a crucial role in the response to spinal cord ***,the precise mechanism remains *** investigate the molecular mechanisms by which microglia regulate the neuroinflammatory response to spinal cord injury,we performed single-cell RNA sequencing dataset analysis,focusing on changes in microglial *** found that the MG1 subpopulation emerged in the acute/subacute phase of spinal cord injury and expressed genes related to cell pyroptosis,sphingomyelin metabolism,and neuroinflammation at high ***,we established a mouse model of contusive injury and performed intrathecal injection of siRNA and molecular inhibitors to validate the role of ceramide synthase 5 in the neuroinflammatory responses and pyroptosis after spinal cord ***,we established a PC12-BV2 cell co-culture system and found that ceramide synthase 5 and pyroptosis-associated proteins were highly expressed to induce the apoptosis of neuron *** ceramide synthase 5 expression in a mouse model of spinal cord injury effectively reduced ***,ceramide synthase 5-induced pyroptosis was dependent on activation of the NLRP3 signaling *** ceramide synthase 5 expression in microglia in vivo reduced neuronal apoptosis and promoted recovery of neurological ***2g7 formed a“bridge”between sphingolipid metabolism and ceramide synthase 5-mediated cell death by inhibiting the NLRP3 signaling ***,these findings suggest that inhibiting ceramide synthase 5 expression in microglia after spinal cord injury effectively suppressed microglial pyroptosis mediated by NLRP3,thereby exerting neuroprotective effects.

关键词： ceramide synthase 5 gasdermin D microglia neuroinflammation NLRP3 nuclear factor kappa B Pla2g7 pyroptosis sphingomyelin metabolism spinal cord injury

Direct and rapid detection of serum amino acid and monoamine neurotransmitters to assist the diagnosis of panic disorder

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Science China Chemistry 2025年第01期 377-384页

作者： Shiwen Liu Xuerui Wan Meng Zhao Jiaqi Wang Weilan wu Linlin You Yonggui Yuan Qian Xu rong Gao Key Laboratory of Environmental Medicine Engineering Ministry of Education School of Public Health Southeast University Key Laboratory of Modern Toxicology of Ministry of Education Department of Hygienic Analysis and Detection School of Public HealthNanjing Medical University Department of Psychosomatics and Psychiatry Zhongda Hospital School of Medicine Southeast University

The measurement of amino acid and monoamine neurotransmitters（NTs） in serum plays a central role in the identification and monitoring of clinical panic disorder（PD）. However, the strong polarity and chemical heterogeneity of NTs make direct extraction and rapid detection extremely challenging. Herein, we developed a strategy that integrated solid phase extraction based on magnetic Fe3O4coated with polydopamine（Fe3O4@PDA） and direct analysis of mass spectrometry in real time（DART-MS） for quantification of 10 NTs. We systematically investigated the optimal conditions of this strategy to achieve good accuracy and precision. Compared with existing magnetic solid phase extraction methods for NTs detection in biological samples, this strategy could adsorb more targets while having similar adsorption efficiencies, and the total process time was reduced by an average of 69.42%. We rapidly determined the metabolic abnormality of 6 types of NTs in the serum of PD patients and demonstrated their diagnostic accuracy. In addition, an outstanding feature of PD in which tryptophan was more metabolized by the KYN pathway and the 5-hydroxytryptamine pathway was inhibited could be elucidated by this strategy. Our results highlighted the potential ability of Fe3O4@PDA coupled with DART-MS to assist in the clinical diagnosis of PD.

关键词：

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低糖玫瑰花脯保质技术研究

食品研究与开发 2018年第14期39卷 202-205页

作者：黄飞何菁王荣顺吴荣书云南农业大学食品科学技术学院云南昆明650000

以低糖玫瑰花脯为研究对象,通过单因素试验筛选出适宜的降水分活度(water activity,Aw)剂,采用二次旋转正交建立降水分活度模型,筛选出降Aw剂组合。结果表明,适宜的降水分活度剂为氯化钠、丙二醇和丙三醇,玫瑰花脯降Aw数学模型:Y(ΔAw)=... 详细信息

以低糖玫瑰花脯为研究对象,通过单因素试验筛选出适宜的降水分活度(water activity,Aw)剂,采用二次旋转正交建立降水分活度模型,筛选出降Aw剂组合。结果表明,适宜的降水分活度剂为氯化钠、丙二醇和丙三醇,玫瑰花脯降Aw数学模型:Y(ΔAw)=0.080 94+0.016 87X1+0.016 45X2+0.008 77X3-0.008 33X22,寻优后降Aw剂组合为:氯化钠1.5%、丙二醇2.0%和丙三醇1.2%,采用该降Aw剂组合实际加工的玫瑰花脯的Aw值为0.663。

关键词：玫瑰花低糖果脯水分活度数学模型

初花期喷镧对UV-B辐射增强下紫花苜蓿光合及荧光特性的影响

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中国农业气象 2017年第4期38卷 230-239页

作者：王伟王岩梁变变赵天宏田荣荣武红艳沈阳农业大学农学院沈阳110866 西北师范大学地理与环境科学学院兰州730070

在温室大棚内采用样方法条播和叶面喷施技术,以紫花苜蓿(Medicago sativa)为试验材料,UV-B辐射增强设5%(T1)和10%(T2)2个梯度,镧(Lanthanum)喷施设30、60和90mg·L-1 3个浓度,以植株上方悬挂灯架但不安装紫外灯管且不喷施镧的处理为对照... 详细信息

在温室大棚内采用样方法条播和叶面喷施技术,以紫花苜蓿(Medicago sativa)为试验材料,UV-B辐射增强设5%(T1)和10%(T2)2个梯度,镧(Lanthanum)喷施设30、60和90mg·L-1 3个浓度,以植株上方悬挂灯架但不安装紫外灯管且不喷施镧的处理为对照(CK),研究UV-B辐射增强下喷施镧对紫花苜蓿光合及荧光特性的影响。结果表明:(1)在UV-B辐射增强条件下,紫花苜蓿叶片叶绿素a(Chla)、叶绿素b(Chlb)、净光合速率(A)、蒸腾速率(E)、气孔导度(gs)、胞间CO2浓度(Ci)、PSⅡ最大光化学量子产量(Fv/Fm)、光化学淬灭(q P)和非光化学淬灭(NPQ)均低于对照,表观光合电子传递效率(ETR)则高于对照(CK),说明UV-B辐射增强会降低紫花苜蓿叶片的光合特性。(2)喷施镧后,紫花苜蓿初花期、盛花期和成熟期叶片以上各指标均相应高于T_1和T_2处理,其中以在UV-B辐射增强5%条件下喷施30mg·L-1和UV-B辐射增强10%条件下喷施60mg·L^(-1)镧效果显著(P<0.05),说明一定浓度的镧可以缓解UV-B辐射增强对紫花苜蓿光合特性造成的伤害,叶片光合作用得以增强;随着生育期的推进,紫花苜蓿叶片可溶性糖和可溶性蛋白含量持续上升,说明UV-B辐射和镧的喷施对紫花苜蓿叶片糖和蛋白的合成有一定促进作用。

关键词：镧 UV-B 紫花苜蓿光合特性叶绿素荧光特性

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术后化疗对早期高危子宫内膜癌预后的影响

江苏医药 2017年第12期43卷 841-844页

作者：荣慧李荣吴强江苏省中医院妇科江苏省南京市210029 南京市妇幼保健院妇产科江苏省肿瘤医院妇瘤科

目的探讨术后辅助化疗对早期高危子宫内膜癌患者预后的影响。方法具有高危因素的早期子宫内膜癌患者213例,术后接受化疗的105例(化疗组),未接受化疗的108例(对照组)。比较两组患者的5年累积生存率。结果化疗组的5年累积生存率为94.2%,... 详细信息

目的探讨术后辅助化疗对早期高危子宫内膜癌患者预后的影响。方法具有高危因素的早期子宫内膜癌患者213例,术后接受化疗的105例(化疗组),未接受化疗的108例(对照组)。比较两组患者的5年累积生存率。结果化疗组的5年累积生存率为94.2%,高于对照组的84.4%(P<0.05)。分层分析显示,子宫内膜癌的病理类型、分期和分级、淋巴脉管间隙浸润和是否行术后辅助化疗是影响患者预后的独立危险因素(P<0.05)。结论术后辅助化疗可以改善具有高危因素的早期子宫内膜癌患者的预后。

关键词：子宫内膜癌化疗