Immunomodulative effects of mesenchymal stem cells derived from human embryonic stem cells in vivo and in vitro

作     者：Zhou TAN Zhong-yuan SU Rong-rong WU Bin GU Yu-kan LIU Xiao-li ZHAO Ming ZHANG 

作者机构：Institute of Cell Biology and Genetics College of Life Sciences Zhejiang University Hangzhou 310058 China Institute of Developmental and Regenerative Biology College of Life and Environmental Sciences Hangzhou Normal University Hangzhou 310036 China 

出 版 物：《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》 (浙江大学学报（英文版）B辑（生物医学与生物技术）)

年 卷 期：2011年第12卷第1期

页      面：18-27页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 071009[理学-细胞生物学] 09[农学] 0901[农学-作物学] 090102[农学-作物遗传育种] 

基　　金：Project (No.2007CB947804) supported by the National Basic Research Program (973) of China 

主　　题：Human embryonic stem cells Mesenchymal stem cells Differentiation Immunomodulative effects 

摘      要：Objective: Human embryonic stem cells (hESCs) have recently been reported as an unlimited source of mesenchymal stem cells (MSCs).The present study not only provides an identical and clinically compliant MSC source derived from hESCs (hESC-MSCs),but also describes the immunomodulative effects of hESC-MSCs in vitro and in vivo for a carbon tetrachloride (CCl4)-induced liver inflammation ***: Undifferentiated hESCs were treated with Rho-associated kinase (ROCK) inhibitor and induced to fibroblast-looking *** cells were tested for their surface markers and multilineage differentiation *** more,we analyzed their immune characteristics by mixed lymphocyte reactions (MLRs) and animal ***: hESC-MSCs show a homogenous fibroblastic morphology that resembles bone marrow-derived MSCs (BM-MSCs).The cell markers and differentiation potential of hESC-MSCs are also similar to those of *** their original cells,hESC-MSCs possess poor immunogenicity and can survive and be engrafted into a xenogenic immunocompetent ***: The hESC-MSCs demonstrate strong inhibitory effects on lymphocyte proliferation in vitro and anti-inflammatory infiltration properties in *** study offers information essential to the applications of hESC-MSC-based therapies and evidence for the therapeutic mechanisms of action.