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Putative zeatin O-glucosyltransferase OscZOG1 regulates root and shoot development and formation of agronomic traits in rice

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Journal of Integrative Plant Biology 2016年第7期58卷 627-641页

作者： Xiao-Ling Shang rong-rong Xie Hua Tian,Qing-Long Wang and fang-Qing Guo rong-rong Xie Hua Tian Qing-Long Wang fang-Qing Guo The National Key Laboratory of Plant Molecular Genetics and National Center of Plant Gene Research(shanghai) Institute of Plant Physiology&EcologyShanghai Institutes for Biological SciencesChinese Academy of SciencesShanghai 200032China.

As a ubiquitous reaction, glucosylation controls the bioactivity of cytokinins in plant growth and development. Here we show that genetic manipulation of zeatin-O- glucosylation regulates the formation of important agronomic traits in rice by manipulating the expression of OscZOG1 gene, encoding a putative zeatin O-glucosyltransferase. We found that OscZOG~ was preferentially expressed in shoot and root meristematic tissues and nascent organs. The growth of lateral roots was stimulated in the overexpression lines, but inhibited in RNA interference lines. In shoots, knockdown of OscZOG1 expression by RNA interference significantly im- proved tillering, panicle branching, grain number per panicle and seed size, which are important agronomic traits for grain yield. In contrast, constitutive expression of OscZOG1 leads to negative effects on the formation of the grain-yielding traits with a marked increase in the accumulation levels of cis-zeatin O-glucoside （cZOG） in the transgenic rice plants. In this study,our findings demonstrate the feasibility of improving the critical yield-determinant agronomic traits, including tiller number, panicle branches, total grain number per panicle and grain weight by downregulating the expression level of OscZOG1. Our results suggest that modulating the levels of cytokinin glucosylation can function as a fine-tuning switch in regulating the formation of agronomic traits in rice.

关键词： Cis-zeatin O-glucoside (cZOG) cytokinin glucosylation grain-yield traits lateral root panicle development rice

Phylogenetically clustering of rhizobia by genome structure:application to unclassified Rhizobium

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Journal of Environmental Sciences 2006年第3期18卷 530-536页

作者： ZHENG Jun-fang LIU Gui-rong LIU Shu-lin Department of Biological Sciences and Biotechnology Tsinghua University Beijing 100084 China Department of Microbiology Peking University Health Science Center Beijing 100083 China

Previous research reveals that the genome structures of rhizobial type strains and reference strains can reflect their phylogenetic relationships. In order to further explore the potential application of genome structure as a phylogenetic marker in rhizobial natural taxonomy, this study analyzed the genome structures of 29 unclassified nodule bacteria isolated from the root nodules of leguminous trees, Robinia sp., Dalbergia spp., and A lbizia spp. and 7 rhizobial reference strains by I-CeuI cleavage, then clustered these bacteria phylogenetically based on their genome structures and compared these clusters with those based on numerical taxonomy and 16S rDNA PCR-RFLP. Eleven phylogenetic clusters were obtained, The clusters were in large part consistent with those based on numerical taxonomy and 16S rDNA PCR-RFLP. Also there are inconsistent clusters based on the above three methods. But results are completely consistent with 16S rRNA clusters. This suggested that the genome structure clustering method can be used to lastly identify root nodule isolates and detect their phylogenetic relationships. The credibility and repeatability of the results, together with the simplicity and possibility to analyze a large number of strains in a short time of the method, indicates the broad potential application of genome structure as phylogenetic marker to categorize rhizobial isolates and should in the future facilitate biodiversity studies.

关键词： rhizobia genome structure phylogenetic analysis pulsed field gel electrophoresis I-CeuI

Simulation methodology development for rotating blade containment analysis

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Journal of Zhejiang University-Science A(Applied Physics & Engineering) 2012年第4期13卷 239-259页

作者： Qing HE Hai-jun XUAN Lian-fang LIAO Wei-rong HONG rong-ren WU High-Speed Rotating Machinery Laboratory Institute of Chemical MachineryZhejiang UniversityHangzhou 310027China Department of Automation Zhejiang UniversityHangzhou 310027China

An experimental and numerical investigation on the aeroengine blade/case containment analysis is presented. Blade out containment capability analysis is an essential step in the new aeroengine design, but containment tests are time-consuming and incur significant costs; thus, developing a short-period and low-cost numerical method is warranted. Using explicit nonlinear dynamic finite element analysis software, the present study numerically investigated the high-speed impact process for simulated blade containment tests which were carried out on high-speed spin testing facility. A number of simulations were conducted using finite element models with different mesh sizes and different values of both the contact penalty factor and the friction coefficient. Detailed comparisons between the experimental and numerical results reveal that the mesh size and the friction coefficient have a considerable impact on the results produced. It is shown that a finer mesh will predict lower containment capability of the case, which is closer to the test data. A larger value of the friction coefficient also predicts lower containment capability. However, the contact penalty factor has little effect on the simulation results if it is large enough to avoid false penetration.

关键词： Aeroengine Blade containment Simulation methodology Mesh size Contact penalty factor Friction coefficient

Sniffing with mass spectrometry

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Science Bulletin 2018年第20期63卷 1351-1357页

作者： Miao-Miao Chen Hai-Feng su Ying Xie Li-fang He Shui-Chao Lin Mei-Lin Zhang Cheng Wang su-Yuan Xie rong-Bin Huang Lan-sun Zheng State Key Laboratory for Physical Chemistry of Solid surfaces Collaborative Innovation Center of Chemistry for Energy Materials Department of Chemistry College of Chemistry and Chemical Engineering Xiamen University School of Information Science and Engineering Xiamen University

Gaseous compounds are usually on-line detectable on sensors. The limitations of conventional sensors are suffering from incapability for exactly identifying multiple components as well as incompatibility to possible toxicants in every odor sample. Herein, we discuss an inlet modification to the laboratory standard mass spectrometer, inspired by the sensitive olfactory systems of animals, for direct sniffing,established by connecting a mini pump to the nebulizer gas tubing. The modified mass spectrometry method—sniffing-mass spectrometry(sniffing-MS)—can acquire detailed fingerprint spectra of mixed odors and shows high tolerance to toxicants. Furthermore, the method has a low limit of detection in the order of parts per trillion and is a ‘sampling-free' technique for analyzing various gaseous compounds simultaneously, thus offering versatility for smelling daily commodities, tracking diffusion, and locating position of odors. Sniffing-MS can mimic or even surpass the olfaction of animals and is applicable for analyzing gaseous/volatile compounds, especially those polar compounds, in a simple manner depending on the intrinsic molecular mass-to-charge ratio.

关键词： Ambient mass spectrometry Volatile compounds Gas source locating On-line monitoring Olfaction

Recursive Approximation of Complex Behaviours With IoT-Data Imperfections

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IEEE/CAA Journal of Automatica Sinica 2020年第3期7卷 656-667页

作者： Korkut Bekiroglu Seshadhri Srinivasan Ethan Png rong su Constantino Lagoa College of Engineering SUNY Polytechnic InstituteUtica 13503NYUSA Berkeley Education Alliance for Research in Singapore(BEARS) Singapore 138602Singapore School of Electrical and Electronic Engineering Nanyang Technological UniversitySingapore 639798Singapore IEEE Electrical Engineering Department of Pennsylvania State University ParkPA 16802 USA

This paper presents an approach to recursively estimate the simplest linear model that approximates the time-varying local behaviors from imperfect(noisy and incomplete) measurements in the internet of things(IoT) based distributed decision-making problems. We first show that the problem of finding the lowest order model for a multi-input single-output system is a cardinality(l0) optimization problem, known to be *** solve the problem a simpler approach is proposed which uses the recently developed atomic norm concept and the modified Frank-Wolfe(mFW) algorithm is introduced. Further, the paper computes the minimum data-rate required for computing the models with imperfect measurements. The proposed approach is illustrated on a building heating, ventilation, and air-conditioning(HVAC) control system that aims at optimizing energy consumption in commercial buildings using IoT devices in a distributed manner. The HVAC control application requires recursive thermal dynamical model updates due to frequently changing conditions and non-linear dynamics. We show that the method proposed in this paper can approximate such complex dynamics on single-board computers interfaced to sensors using unreliable communication channels. Real-time experiments on HVAC systems and simulation studies are used to illustrate the proposed method.

关键词： Adaptability distributed decision systems imperfect measurements internet of things(IoT) low order model identification

Excellent effects and possible mechanisms of action of a new antibody–drug conjugate against EGFR-positive triple-negative breast cancer

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Military Medical Research 2022年第4期9卷 419-431页

作者： Dan-Dan Zhou Wei-Qi Bai Xiao-Tian Zhai Li-Ping sun Yong-su Zhen Zhuo-rong Li Qing-fang Miao NHC Key Laboratory of Biotechnology of Antibiotics Institute of Medicinal BiotechnologyChinese Academy of Medical Sciences and Peking Union Medical CollegeNo.1 Tiantan XilBeijing 100050China Department of Organic Chemistry Institute of Medicinal BiotechnologyChinese Academy of Medical Sciences and Peking Union Medical CollegeNo.1 Tiantan XiliBeijing 100050China

Background:Triple-negative breast cancer(TNBC)is the most aggressive subtype and occurs in approximately 15%–20%of diagnosed breast *** is characterized by its highly metastatic and recurrent features,as well as a lack of specific targets and targeted *** growth factor receptor(EGFR)is highly expressed in a variety of tumors,especially in ***004-VC-MMAE is a new EGFR-targeting antibody–drug conjugate produced by our *** study aimed to evaluate its antitumor activities against EGFR-positive TNBC and further studied its possible mechanism of antitumor ***:LR004-VC-MMAE was prepared by coupling a cytotoxic payload(MMAE)to an anti-EGFR antibody(LR004)via a linker,and the drug-to-antibody ratio(DAR)was analyzed by *** gene expression of EGFR in a series of breast cancer cell lines was assessed using a publicly available microarray dataset(GSE41313)and Western ***-MB-468 and MDA-MB-231 cells were treated with LR004-VC-MMAE(0,0.0066,0.066,0.66,6.6 nmol/L),and the inhibitory effects of LR004-VC-MMAE on cell proliferation were examined by CCK-8 and colony *** migration and invasion capacity of MDA-MB-468 and MDA-MB-231 cells were tested at different LR004-VCMMAE concentrations(2.5 and 5 nmol/L)with wound healing and Transwell invasion *** cytometric analysis and tumorsphere-forming assays were used to detect the killing effects of LR004-VC-MMAE on cancer stem cells(MDA-MB-468 and MDA-MB-231 cells).The mouse xenograft models were also used to evaluate the antitumor efficacy of LR004-VC-MMAE in ***,BALB/c nude mice were subcutaneously inoculated with MDA-MB-468 or MDAMB-231 *** they were randomly divided into 4 groups(n=6 per group)and treated with PBS,naked LR004(10 mg/kg),LR004-VC-MMAE(10 mg/kg),or doxorubicin,*** sizes and the body weights of mice were measured every 4 *** effects of LR004-VC-MMAE on apoptosis and cell cycle distribution were analyzed

关键词： Triple-negative breast cancer Epidermal growth factor receptor Antibody–drug conjugate Targeted therapy Antitumor effect

Effect of synbiotics on intestinal microflora and digestive enzyme activities in rats

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World Journal of Gastroenterology 2005年第47期11卷 7413-7417页

作者： suh-Ching Yang Ju-Yen Chen Huey-fang Shang Ting-Ying Cheng su Chen Tsou Jiun-rong Chen School of Nutrition and Health Sciences College of Public Health and NutritionTaipei Medical UniversityTaipeiTaiwanChina Department of Microbiology and Immunology School of MedicineTaipei Medical UniversityTaipeiTaiwanChina Viva Life Science/Westar Nutrition Costa MesaCAUnited States

AIM: To investigate the effect of synbiotics, i.e. probiotics and prebiotics mixture, on the gut microbial ecology and digestive enzyme activities in rats. METHODS: Forty-eight SD rats weighing about 280 g were used in this study. Rats were divided into three groups according to the contents of probiotics and prebiotics mixture in the feed as control, low and high dose groups. The duration of the experiment was 8 wk. REsuLTS: Compared with the control group, thefecal Lactobacillus and Bifidobacterium counts were significantly increased and the fecal Coliform organism counts were markedly reduced in the low and high dose groups. Concerning the digestive enzyme activity of jejunum, only lactase activity increased in low dose group. However, significant increase of lipase, lactase, sucrase, and isomaltase activities were observed in high dose ***: Intake of low and high dosages of probiotics and prebiotics mixture significantly improved the ecosystem of the intestinal tract by increasing the probiotics population and digestive enzyme activities in rats.

关键词： Synbiotics Intestinal microflora Digestive enzyme activity

Optimum Metallic-Bond Scheme: A Quantitative Analysis of Mass Spectra of Sodium Clusters

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Chinese Physics Letters 2001年第12期18卷 1575-1577页

作者： su Chang-rong LI Jia-Ming Department of Physics Center for Atomic and Molecular NanosciencesTsinghua UniversityBeijing 100084 Institute of Physics Chinese Academy of SciencesBeijing 100080

Based on the results of the optimum metallic-bond scheme for sodium clusters, we present a quantitative analysis of the detailed features of the mass spectra of sodium clusters. We find that, in the generation of sodium clusters with various abundances, the quasi-steady processes through adding or losing a sodium atom dominate. The quasi-steady processes through adding or losing a sodium dimer are also important to understand the detailed features of mass spectra for small clusters.

关键词： adding quasi spectra

Quasi-consistent fusion navigation algorithm for DSS

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Science China(Information Sciences) 2018年第1期61卷 187-203页

作者： Qiya su Yi HUANG Yanguang JIANG Haitao fang Key Laboratory of Systems and Control Academy of Mathematics and Systems ScienceChinese Academy of Sciences School of Mathematical Sciences University of Chinese Academy of Sciences Beijing Institute of Control and Electronics Technology

A fusion navigation algorithm for the distributed satellites system(DSS) utilizing relative range measurements is proposed in this paper. Based on the quasi-consistent extended Kalman filter(QCEKF), an on-line evaluation of the navigation precision can be provided by the fusion navigation algorithm. In addition,the upper bound for the estimation error obtained from the fusion navigation algorithm is lower than those with any groups of measurements, which indicates that the fusion navigation algorithm can automatically choose the suitable redundant measurements to improve the navigation precision. The simulations show the feasibility and effectiveness of the proposed fusion navigation algorithm.

关键词： quasi-consistent extended Kalman filter(QCEKF) extended Kalman filter(EKF) fusion algorithm navigation distributed satellites system(DSS)